The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform
Hiroshi Sentsui - One of the best experts on this subject based on the ideXlab platform.
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Serological survey of arthropod-borne Viruses among wild boars in Japan.
Journal of Veterinary Medical Science, 2009Co-Authors: Iku Sugiyama, Eisuke Shimizu, Kazuhiko Suzuki, Sadao Nogami, Yasuo Miura, Hiroshi SentsuiAbstract:A total of 90 blood samples were collected from wild boars in the Kyushu region of Japan, and a seroepidemiological survey for 7 arthropod-borne Viruses was performed using hemagglutination inhibition tests. The individual seropositive rates for each Virus were 52.2% for Japanese encephalitis Virus (JEV), 47.8% for Getah Virus (GETV), 13.3% for Akabane Virus, 10.0% for Aino Virus and less than 5% for Bluetongue Virus, Chuzan Virus and Ibaraki Virus. The results indicated that among the 7 Viruses, JEV and GETV infections were prevalent among the wild boars and that the animals were involved in the natural transmission cycle of JEV and GETV in Japan. It is necessary to consider the participation of wild animals for the control of arthropod-borne Virus infections.
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Genomic analysis of some Japanese isolates of Getah Virus.
Veterinary microbiology, 2001Co-Authors: Sabenzia N Wekesa, Yasuo Inoshima, Kenji Murakami, Hiroshi SentsuiAbstract:Using the reverse transcription-polymerase chain reaction (RT-PCR) and direct sequencing, capsid protein and non-structural protein 1 (nsP1) regions of Sagiyama Virus and eight Getah Virus strains were analysed. The Viruses were isolated from Malaysia and various areas of Japan over a period of 30 years. Based on the available published sequence data, oligonucleotide primers were designed for RT-PCR and the sequences were determined. Our findings showed that though there were differences in the nucleotide sequences in the nsP1 region, there was 100% amino acid homology. On the other hand, in the capsid region, the nucleotide differences caused a major difference in the amino acid sequence. Therefore, the difference in the capsid region is one of the useful markers in the genetic classification between Sagiyama Virus and strains of Getah Virus, and might be responsible for the serological difference in complement fixation test. The genomic differences among the Getah Virus strains are due to time factor rather than geographical distribution.
Kazuo Suzuki - One of the best experts on this subject based on the ideXlab platform.
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Getah Virus epizootic among wild boars in Japan around 2012
Archives of Virology, 2018Co-Authors: Ryusei Kuwata, Hiroshi Shimoda, Thanmaporn Phichitraslip, Noppadol Prasertsincharoen, Keita Noguchi, Kenzo Yonemitsu, Shohei Minami, Ngo Thuy Bao Tran, Ai Takano, Kazuo SuzukiAbstract:In 2014, an outbreak of Getah Virus (GETV) infection occurred in Japan in a horse population that was inoculated with a vaccine against GETV. In this study, we investigated the seroprevalence of GETV infection among wild boars in Japan. Interestingly, the highest rate of anti-GETV-positive wild boars was observed in 2013, which gradually decreased during 2014–2016. The results suggested that GETV spread among wild boars around 2012, resulting in the 2014 outbreak.
Takashi Kondo - One of the best experts on this subject based on the ideXlab platform.
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Complete Genome Sequences of Getah Virus Strains Isolated from Horses in 2016 in Japan.
Genome announcements, 2017Co-Authors: Manabu Nemoto, Hiroshi Bannai, Koji Tsujimura, Takashi Yamanaka, Akihiro Ochi, Hiroshi Kokado, Hidekazu Niwa, Satoshi Murakami, Takashi KondoAbstract:ABSTRACT Getah Virus is mosquito-borne and causes disease in horses and pigs. We sequenced and analyzed the complete genomes of three strains isolated from horses in Ibaraki Prefecture, eastern Japan, in 2016. They were almost identical to the genomes of strains recently isolated from horses, pigs, and mosquitoes in Japan.
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geospatial and temporal associations of Getah Virus circulation among pigs and horses around the perimeter of outbreaks in japanese racehorses in 2014 and 2015
BMC Veterinary Research, 2017Co-Authors: Hiroshi Bannai, Koji Tsujimura, Manabu Nemoto, Takashi Yamanaka, Hidekazu Niwa, Satoshi Murakami, Takashi KondoAbstract:We studied a recent epizootic of Getah Virus infection among pigs in the southern part of Ibaraki Prefecture and the northern part of Chiba Prefecture, Japan, focusing on its possible association with outbreaks in racehorses in 2014 and 2015. The genomic sequence of a Getah Virus strain from an infected pig was analyzed to evaluate the degree of identity with the strains from horses. Sera were collected from pigs from September to December 2012 to 2015 in south Ibaraki (380 pigs in 29 batches), and from September to December 2010 to 2015 in north Chiba (538 pigs in 104 batches). They were examined by using a Virus-neutralizing test for Getah Virus. Seropositivity rates in 2012–2013 in south Ibaraki and 2010–2012 in north Chiba ranged from 0% to 1.6%. In south Ibaraki, seropositivity rates in 2014 (28.8%) and 2015 (65.0%) were significantly higher than those in the previous years (P < 0.01); 4/5 batches had positive sera in 2014 and 7/7 in 2015. In north Chiba, seropositivity rates in 2013 (14.1%), 2014 (17.8%), and 2015 (48.0%) were significantly higher than those in the previous years (P < 0.01); 6/27 batches had positive sera in 2013, 3/9 in 2014, and 5/5 in 2015. Complete genome analysis revealed that the Virus isolated from an infected pig had 99.89% to 99.94% nucleotide identity to the strains isolated from horses during the outbreaks in 2014 and 2015. Serological surveillance of Getah Virus in pigs revealed that the Virus was circulating in south Ibaraki and north Chiba in 2014 and 2015; this was concomitant with the outbreaks in racehorses. The Getah Virus strain isolated from a pig was closely related to the ones from horses during the 2014 and 2015 outbreaks. To our knowledge, this is the first convincing case of simultaneous circulation of Getah Virus both among pigs and horses in specific areas.
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Geospatial and temporal associations of Getah Virus circulation among pigs and horses around the perimeter of outbreaks in Japanese racehorses in 2014 and 2015
BMC Veterinary Research, 2017Co-Authors: Hiroshi Bannai, Koji Tsujimura, Manabu Nemoto, Takashi Yamanaka, Hidekazu Niwa, Satoshi Murakami, Takashi KondoAbstract:Background We studied a recent epizootic of Getah Virus infection among pigs in the southern part of Ibaraki Prefecture and the northern part of Chiba Prefecture, Japan, focusing on its possible association with outbreaks in racehorses in 2014 and 2015. The genomic sequence of a Getah Virus strain from an infected pig was analyzed to evaluate the degree of identity with the strains from horses. Results Sera were collected from pigs from September to December 2012 to 2015 in south Ibaraki (380 pigs in 29 batches), and from September to December 2010 to 2015 in north Chiba (538 pigs in 104 batches). They were examined by using a Virus-neutralizing test for Getah Virus. Seropositivity rates in 2012–2013 in south Ibaraki and 2010–2012 in north Chiba ranged from 0% to 1.6%. In south Ibaraki, seropositivity rates in 2014 (28.8%) and 2015 (65.0%) were significantly higher than those in the previous years ( P
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Geospatial and temporal associations of Getah Virus circulation among pigs and horses around the perimeter of outbreaks in Japanese racehorses in 2014 and 2015
BMC veterinary research, 2017Co-Authors: Hiroshi Bannai, Koji Tsujimura, Manabu Nemoto, Takashi Yamanaka, Hidekazu Niwa, Satoshi Murakami, Takashi KondoAbstract:We studied a recent epizootic of Getah Virus infection among pigs in the southern part of Ibaraki Prefecture and the northern part of Chiba Prefecture, Japan, focusing on its possible association with outbreaks in racehorses in 2014 and 2015. The genomic sequence of a Getah Virus strain from an infected pig was analyzed to evaluate the degree of identity with the strains from horses. Sera were collected from pigs from September to December 2012 to 2015 in south Ibaraki (380 pigs in 29 batches), and from September to December 2010 to 2015 in north Chiba (538 pigs in 104 batches). They were examined by using a Virus-neutralizing test for Getah Virus. Seropositivity rates in 2012–2013 in south Ibaraki and 2010–2012 in north Chiba ranged from 0% to 1.6%. In south Ibaraki, seropositivity rates in 2014 (28.8%) and 2015 (65.0%) were significantly higher than those in the previous years (P
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a 2015 outbreak of Getah Virus infection occurring among japanese racehorses sequentially to an outbreak in 2014 at the same site
BMC Veterinary Research, 2016Co-Authors: Hiroshi Bannai, Koji Tsujimura, Manabu Nemoto, Takashi Yamanaka, Akihiro Ochi, Takashi KondoAbstract:Background As we reported previously, Getah Virus infection occurred in horses at the Miho training center of the Japan Racing Association in 2014. This was the first outbreak after a 31-year absence in Japan. Here, we report a recurrent outbreak of Getah Virus infection in 2015, sequential to the 2014 one at the same site, and we summarize its epizootiological aspects to estimate the risk of further outbreaks in upcoming years.
Lei Cao - One of the best experts on this subject based on the ideXlab platform.
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Serological Survey of Getah Virus in Domestic Animals in Yunnan Province, China
Vector borne and zoonotic diseases (Larchmont N.Y.), 2018Co-Authors: Xiaofang Guo, Lihua Wang, Wenwen Lei, Xiaoyan Gao, Lei CaoAbstract:Abstract We detected neutralizing antibodies of Getah Virus (GETV) in serum specimens of domestic animals collected from Yunnan Province in China. Antibodies were detected in serum specimens of chicken, duck, dairy cattle, pig, and beef cattle. The positive rate of antibodies in pig and beef cattle was high (46–72%), with titers of 1:640–1:2560. These results suggest that there may be a large number of host animals for GETV in the local area. It is important to improve the monitoring of the incidence of GETV infection in domestic animals, in particular among pigs and beef cattle, by surveillance for animal illness and testing of sick animals.
Guodong Liang - One of the best experts on this subject based on the ideXlab platform.
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emerging of japanese encephalitis Virus and Getah Virus from specimen of mosquitoes in inner mongolia autonomous region
Chinese journal of epidemiology, 2020Co-Authors: Rui Cheng, Huanyu Wang, Xiaowei Nan, Na Fan, Lin Zhang, Wenwen Lei, Guodong LiangAbstract:Objective To investigate the types and distribution of blood-sucking insects and arboViruses in Inner Mongolia autonomous region, and provide basic data for the prevention of arboVirus transmitted disease. Methods Blood-sucking insects were collected by lamp trapping method in nature. Mosquito samples were classified according to morphologic characteristics and then stored at liquid nitrogen. Viruses were isolated in cell culture and characterized, using molecular biological methods. Results A total of 24 240 mosquitoes and 17 110 aphids were collected from 2 sites of 5 counties (Flags) in Inner Mongolia in 2014 and during 2017-2018. Among them, Japanese encephalitis Virus gene was detected in Culex pipiens pallens, and 4 Virus strains isolates which could be stably passaged. The isolates were identified as Getah Virus and densonucleosis Virus by molecular biology identification. Phylogenetic analysis on the E2 gene of the Getah Virus (NMDK1813-1) showed that it belonged to the same evolutionary branch of the Gansu isolates (GS10-2) and having six common amino acid variation sites. Conclusions The emergence of Japanese encephalitis Virus and Getah Virus from specimen of mosquitoes in Inner Mongolia indicated the new challenges on the prevention and control of arboVirus and related diseases. The results pf this study provided basic data for the prevention and control stretagies of arboVirus transmitted diseases in Inner Mongolia. Key words: Japanese encephalitis; Getah Virus; ArboVirus
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First isolation and identification of Getah Virus SC1210 in Sichuan
Chinese Journal of Clinical Hepatology, 2017Co-Authors: Ming Pan, Guodong Liang, Xingyu Zhou, Shihua Lin, Xue-cheng Liu, Dan-lin Chen, Yi-ou Cao, Jiake ZhangAbstract:Objective To study the genome molecular characteristics of Getah Virus (SC1210) which isolated in Sichuan province in 2012. Methods Reverse transcriptase polymerase chain reaction (RT-PCR) was used to identify the isolate and the genome was sequenced by the second Ion Torrent PGM. Computer softwares, including Mega Align and Mega 6, were used to analyze the nucleotide and deduced amino acid sequence, and draw phylogenetic trees. Results SC1210 was identified as Getah Virus. The full genome sequence was 11 690nt, the nucleotide and amino acid homology of the full sequence with other strains were 99.2%-99.7% and 96.5%-99.4%.The capsid protein of SC1210 consisting of 804 nucleotides, encoding 268 amino acids and the full-length of E2 protein, had 1 266 nucleotides, encoding 422 amino acids. The nucleotide homology of the capsid protein and the E2 protein with other strains were 94.9%-99.2% and 94.6%-99.6%, and the amino acid were 97%-99.6% and 97.1%-99.5%. The 3′ UTR of the Virus included 402 nucleotides and there were three repeat sequence elements and 19 nucleotides conservation sequence. Conclusions The first GETV isolate SC1210 in Sichuan province has a closer relationship with Yunnan strain YN040 and a far genetic relationship with MM2021. Key words: Getah Virus; Capsid gene; E2 protein gene; 3′ untranslated region; Nucleotide sequence
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Isolation and identification of mosquito-borne arboViruses in Yuncheng city, Shanxi province, 2012
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi, 2015Co-Authors: Yayun Zheng, Yuxi Cao, Jingxia Cheng, J Y Zhao, Pei-fang Dai, Xiang-sheng Kong, Guodong LiangAbstract:Objective To investigate the species and distribution of mosquitoes and mosquito-borne arboViruses in Yuncheng city of Shanxi province, China. Methods Mosquito samples were collected in 19 collection sites from Linyi county and Yongji city in Yuncheng city, in August, 2012. After identification and classification, all the specimens were homogenized and centrifuged to acquire supernatant before being inoculated to both C6/36 and BHK21 cells for viral isolation. Positive isolates were identified with arboVirus species-specific primers under RT-PCR, for further sequencing and phylogenetic analysis. Results A total of 10 455 mosquitoes of 7 species in 4 genuese were collected. The predominant mosquito species in Linyi county was Culex pipens pallens(91.96%,3 911/4 253) ,but the one in Yongji city was Culex tritaeniorhynchus (72.85%,4 518/6 202). A total of 23 strains of Viruses were isolated from the mosquito pools. 15 strains from Culex tritaeniorhynchus and Culex pipens pallens were identified as genotype Ⅰ Japanese encephalitis Virus (JEV). Four strains from Culex pipens pallens were identified as Culex flaviVirus (CxFV). Three strains from Culex pipens pallens were identified as Culex pipiens pallens densoVirus (CppDNV). One strain from Armigeres subalbatus and Aedes albopictus was identified as Getah Virus (GETV). Conclusion Four kinds of arboViruses were isolated from the mosquito pools, including GETV and CxFV, which were isolated and documented in Shanxi province for the first time. In the city of Yuncheng, Culex tritaeniorhynchus had been the predominant species and major vector for transmitting JEV. Genotype Ⅰ JEV remained the major JEV circulating in the local natural environment. Key words: Mosquito-borne arboViruses; Japanese encephalitis Virus; Getah Virus; Culex flaviVirus
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Partial genome molecular characteristics of Getah Virus newly isolated in China
Chinese journal of microbiology and immunology, 2010Co-Authors: Wei-xin Chen, Huanyu Wang, Hong-yue Jiang, Lihua Wang, Zhi-yu Wang, Guodong LiangAbstract:Objective To study the genome molecular characteristics of Getah Virus(DY0824)which isolated in Shandong province,2008 by molecular biology methods.Methods Reverse transcriptasepolymerase chain reaction(RT-PCR)was used to amplify the structural gene and 3'UTR fragments then the RT-PCR products were inserted into PGEM-T easy to be sequenced.Computer software was used to analyze the nucleotide and deduced amino acid sequence,and draw phylogenetic trees,including Clustal X1.83 and MegaAlign and Mega4.Results The capsid protein of DY0824 consists of 804 nucleotides,encoding 268 amino acids and the full-length of E2 protein is 1266 nucleotides,encoding 422 amino acids.The nucleotide homology of the capsid protein and the E2 protein with other strains were 95.4%-99.9%and 94.8%-99.5%,and the amino acid were 97.4%-100%and 97.6%-100%.The 3'UTR of the Virus include 401 nucleotides and there are three repeat sequence elements.Conclusion Compared with the prototype Virus,the Getah Virus isolated in Shandong province had 7 amino acid differences in capsid protein genes and 10 amino acid differences in E protein genes.The 3'UTR region had multi-nucleotide changes. Key words: Getah Virus; Capsid gene; E2 protein gene; 3' untranslated region; Nucleotide sequence
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complete sequence characterization of isolates of Getah Virus genus alphaVirus family togaviridae from china
Journal of General Virology, 2008Co-Authors: Yougang Zhai, Huanyu Wang, Xiaohong Sun, Huanqin Wang, Houssam Attoui, Qing Tang, Guodong LiangAbstract:Ten Virus isolates belonging to species Getah Virus (GETV) have been obtained during surveys for arboViruses in China since 1964. Seven of these isolates (YN0540, YN0542, SH05-6, SH05-15, SH05-16, SH05-17 and GS10-2) were obtained during the current study. The full-length sequences of three Chinese isolates (M1, isolated in 1964; HB0234, isolated in 2002; YN0540, isolated in 2005) were determined. The full-length sequences of these isolates were respectively 11 696, 11 686 and 11 690 nt, and showed more than 97 % intraspecies identity. Deletions were found in the capsid protein of strain M1 and non-structural protein nsP3 of strain HB0234. The E2 gene and 3′ UTR of all ten isolates were also characterized. The E2 gene of the Chinese GETV isolates showed nucleotide sequence identities of 98–100 % when compared with other GETV isolates. In the 3′ UTR of the Chinese isolates, an insertion of 10 consecutive adenine residues (nt 189–198) appeared in strain M1, and 9 or 3 consecutive adenines were found towards the 3′ end of the third RES in strains SH05-6 and SH05-15, respectively. The 3′ UTRs of the Chinese isolates showed a deletion between positions 45 and 54 and nucleotide transitions at positions 43, 64 and 148. Sequence and phylogenetic analyses showed that there was a relatively high degree of conservation among GETV isolates. The isolation of GETV from various provinces in China and also in Russia and Mongolia (including regions of the northern tundra) are an indication of changes in the world distribution of this re-emerging Virus.