The Experts below are selected from a list of 7245 Experts worldwide ranked by ideXlab platform
Karim Fawzy M Elsayed - One of the best experts on this subject based on the ideXlab platform.
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isolation and characterisation of human Gingival Margin derived stro 1 macs and macs cell populations
International Journal of Oral Science, 2015Co-Authors: Sebastian Paris, Christian Graetz, Neemat Kassem, Mohamed Mekhemar, Hendrick Ungefroren, Fred Fändrich, Karim Fawzy M Elsayed, Christof DörferAbstract:Recently, Gingival Margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo. As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS+) and STRO-1-negative (MACS−) cell populations from the human free Gingival Margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS+ and MACS− cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS+ and MACS− cell fractions showed plastic adherence. MACS+ cells, in contrast to MACS− cells, showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs (P<0.01). More than 95% of MACS+ cells expressed CD105, CD90 and CD73; lacked the haematopoietic markers CD45, CD34 and CD14, and expressed STRO-1 and CD146/MUC18. MACS− cells showed a different surface marker expression profile, with almost no expression of CD14 or STRO-1, and more than 95% of these cells expressed CD73, CD90 and CD146/MUC18, as well as the haematopoietic markers CD34 and CD45 and CD105. MACS+ cells could be differentiated along osteoblastic, adipocytic and chondroblastic lineages. In contrast, MACS− cells demonstrated slight osteogenic potential. Unstimulated MACS+ cells showed significantly higher expression of collagen I (P<0.05) and collagen III (P<0.01), whereas MACS− cells demonstrated higher expression of osteonectin (P<0.05; Mann–Whitney). The present study is the first to compare Gingival MACS+ and MACS− cell populations demonstrating that MACS+ cells, in contrast to MACS− cells, harbour stem/progenitor cell characteristics. This study also validates the effectiveness of the STRO-1/MACS+ technique for the isolation of Gingival stem/progenitor cells. Human free Gingival Margin-derived STRO-1/MACS+ cells are a unique renewable source of multipotent stem/progenitor cells.
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periodontal regeneration employing Gingival Margin derived stem progenitor cells in conjunction with il 1ra hydrogel synthetic extracellular matrix
Journal of Clinical Periodontology, 2015Co-Authors: Karim Fawzy M Elsayed, Stephan T Becker, Mohamed Mekhemar, Benedicta E Beckbroichsitter, Telse Bahr, Marwa Hegab, Jan Receveur, Carola Heneweer, Joerg WiltfangAbstract:AIM: This study investigated the periodontal regenerative potential of Gingival Margin-derived stem/progenitor cells (G-MSCs) in conjunction with IL-1ra-releasing hyaluronic acid synthetic extracellular matrix (HA-sECM). MATERIALS AND METHODS: Periodontal defects were induced at four sites in eight miniature pigs in the premolar/molar area (-4 weeks). Autologus G-MSCs were isolated from the free Gingival Margin and magnetically sorted, using anti-STRO-1 antibodies. Colony formation and multilineage differentiation potential were tested. The G-MSCs were expanded and incorporated into IL-1ra-loaded/unloaded HA-sECM. Within every miniature pig, four periodontal defects were randomly treated with IL-1ra/G-MSCs/HA-sECM (test group), G-MSCs/HA-sECM (positive-control), scaling and root planing (SRP; negative control-1) or left untreated (no-treatment group; negative control 2). Differences in clinical attachment level (ΔCAL), probing depth (ΔPD), Gingival recession (ΔGR), radiographic defect volume (ΔRDV), and changes in bleeding on probing (BOP) between baseline and 16 weeks post-transplantation, as well as periodontal attachment level (PAL), junctional epithelium length (JE), connective tissue adhesion (CTA), cementum regeneration (CR) and bone regeneration (BR) at 16 weeks post-transplantation were evaluated. RESULTS: Isolated G-MSCs showed stem/progenitor cell characteristics. IL-1ra loaded and unloaded G-MSCs/HA-sECM showed higher ΔCAL, ΔPD, ΔGR, PAL, CR and BR as well as a lower JE compared to their negative controls and improved BOP. CONCLUSION: G-MSCs in conjunction with IL-1ra-loaded/unloaded HA-sECM show a significant periodontal regenerative potential.
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periodontal regeneration employing Gingival Margin derived stem progenitor cells an animal study
Journal of Clinical Periodontology, 2012Co-Authors: Karim Fawzy M Elsayed, Sebastian Paris, Stephan T Becker, Matthias Neuschl, Wiebke De Buhr, Sonja Salzer, Andreas Wulff, Mahmoud I Elrefai, Mona S Darhous, Manal ElmasryAbstract:Aim This study investigated the periodontal regenerative potential of Gingival Margin-derived multipotent postnatal stem/progenitor cells. Material and Methods Periodontal defects were induced at six sites in eight miniature pigs in the premolar/molar area (−4 weeks). Autologous cells isolated from the Gingival Margin were magnetically sorted using STRO-1 antibodies and characterized flow cytometrically for the expression of CD14, CD31, CD34, CD45, CD117 and STRO-1 surface markers. Colony formation and multilineage differentiation potential were tested. The cells were expanded and loaded on deproteinized bovine cancellous bone (DBCB) and Collagen scaffolds. Within every miniature pig, six periodontal defects were randomly treated with loaded-DBCB (test group 1), unloaded-DBCB (control group 1), loaded-Collagen scaffolds (test group 2), unloaded-Collagen scaffolds (control group 1), scaling and root planing (negative control 1) or left untreated (negative control 2). Differences in clinical attachment level (ΔCAL), probing depth (ΔPD), Gingival recession (ΔGR) and radiographic defect volume (ΔRDV) between baseline and 12 weeks, as well as histological attachment level (HAL), junctional epithelium length (JE) and connective tissue adhesion (CTA) after 12 weeks were evaluated. Results Isolated cells showed stem/progenitor cell characteristics. Cell-loaded scaffolds showed higher ΔCAL, ΔPD, ΔGR, HAL and lower JE and CTA compared with unloaded scaffolds and negative controls. The sort of scaffold had no significant influence on the measured outcomes. Conclusion Gingival Margin-derived stem/progenitor cells show significant periodontal regenerative potential.
Christof Dörfer - One of the best experts on this subject based on the ideXlab platform.
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tlr expression profile of human Gingival Margin derived stem progenitor cells
Medicina Oral Patologia Oral Y Cirugia Bucal, 2016Co-Authors: Karim Fawzyelsayed, Mohamed Mekhemar, Sabine Adamklages, Dietrich Kabelitz, Christof DörferAbstract:Background: Gingival Margin-derived stem/progenitor cells (G-MSCs) show remarkable periodontal regenerative potential in vivo. During regeneration, G-MSCs may interact with their inflammatory environment via toll-likereceptors (TLRs). The present study aimed to depict the G-MSCs TLRs expression profile. Material and Methods: Cells were isolated from free Gingival Margins, STRO-1-immunomagnetically sorted and seeded to obtain single colony forming units (CFUs). G-MSCs were characterized for CD14, CD34, CD45, CD73, CD90, CD105, CD146 and STRO-1 expression, and for multilineage differentiation potential. Following G-MSCs’ incubation in basic or inflammatory medium (IL-1β, IFN-γ, IFN-α, TNF-α) a TLR expression profile was generated. Results: G-MSCs showed all stem/progenitor cells’ characteristics. In basic medium G-MSCs expressed TLRs 1, 2, 3, 4, 5, 6, 7, and 10. The inflammatory medium significantly up-regulated TLRs 1, 2, 4, 5, 7 and 10 and diminished TLR 6 (p≤0.05, Wilcoxon-Signed-Ranks-Test). Conclusions: The current study describes for the first time the distinctive TLRs expression profile of G-MSCs under uninflamed and inflamed conditions.
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isolation and characterisation of human Gingival Margin derived stro 1 macs and macs cell populations
International Journal of Oral Science, 2015Co-Authors: Sebastian Paris, Christian Graetz, Neemat Kassem, Mohamed Mekhemar, Hendrick Ungefroren, Fred Fändrich, Karim Fawzy M Elsayed, Christof DörferAbstract:Recently, Gingival Margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo. As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS+) and STRO-1-negative (MACS−) cell populations from the human free Gingival Margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS+ and MACS− cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS+ and MACS− cell fractions showed plastic adherence. MACS+ cells, in contrast to MACS− cells, showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs (P<0.01). More than 95% of MACS+ cells expressed CD105, CD90 and CD73; lacked the haematopoietic markers CD45, CD34 and CD14, and expressed STRO-1 and CD146/MUC18. MACS− cells showed a different surface marker expression profile, with almost no expression of CD14 or STRO-1, and more than 95% of these cells expressed CD73, CD90 and CD146/MUC18, as well as the haematopoietic markers CD34 and CD45 and CD105. MACS+ cells could be differentiated along osteoblastic, adipocytic and chondroblastic lineages. In contrast, MACS− cells demonstrated slight osteogenic potential. Unstimulated MACS+ cells showed significantly higher expression of collagen I (P<0.05) and collagen III (P<0.01), whereas MACS− cells demonstrated higher expression of osteonectin (P<0.05; Mann–Whitney). The present study is the first to compare Gingival MACS+ and MACS− cell populations demonstrating that MACS+ cells, in contrast to MACS− cells, harbour stem/progenitor cell characteristics. This study also validates the effectiveness of the STRO-1/MACS+ technique for the isolation of Gingival stem/progenitor cells. Human free Gingival Margin-derived STRO-1/MACS+ cells are a unique renewable source of multipotent stem/progenitor cells.
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Isolation and characterisation of human Gingival Margin-derived STRO-1/MACS + and MACS − cell populations
International Journal of Oral Science, 2015Co-Authors: Karim M Fawzy El-sayed, Sebastian Paris, Christian Graetz, Neemat Kassem, Mohamed Mekhemar, Hendrick Ungefroren, Fred Fändrich, Christof DörferAbstract:Recently, Gingival Margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo. As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS+) and STRO-1-negative (MACS−) cell populations from the human free Gingival Margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS+ and MACS− cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS+ and MACS− cell fractions showed plastic adherence. MACS+ cells, in contrast to MACS− cells, showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs (P
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Isolation and characterisation of human Gingival Margin-derived STRO-1/MACS^+ and MACS^− cell populations
International Journal of Oral Science, 2015Co-Authors: Karim M Fawzy El-sayed, Sebastian Paris, Christian Graetz, Neemat Kassem, Mohamed Mekhemar, Hendrick Ungefroren, Fred Fändrich, Christof DörferAbstract:Recently, Gingival Margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo . As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS^+) and STRO-1-negative (MACS^−) cell populations from the human free Gingival Margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS^+ and MACS^− cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS^+ and MACS^− cell fractions showed plastic adherence. MACS^+ cells, in contrast to MACS^− cells, showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs ( P
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Periodontal regeneration employing Gingival Margin‐derived stem/progenitor cells in conjunction with IL‐1ra‐hydrogel synthetic extracellular matrix
Journal of Clinical Periodontology, 2015Co-Authors: Karim M Fawzy El-sayed, Mohamed Mekhemar, Stephan T Becker, Telse Bahr, Marwa Hegab, Jan Receveur, Carola Heneweer, Joerg Wiltfang, Benedicta E. Beck-broichsitter, Christof DörferAbstract:AIM: This study investigated the periodontal regenerative potential of Gingival Margin-derived stem/progenitor cells (G-MSCs) in conjunction with IL-1ra-releasing hyaluronic acid synthetic extracellular matrix (HA-sECM). MATERIALS AND METHODS: Periodontal defects were induced at four sites in eight miniature pigs in the premolar/molar area (-4 weeks). Autologus G-MSCs were isolated from the free Gingival Margin and magnetically sorted, using anti-STRO-1 antibodies. Colony formation and multilineage differentiation potential were tested. The G-MSCs were expanded and incorporated into IL-1ra-loaded/unloaded HA-sECM. Within every miniature pig, four periodontal defects were randomly treated with IL-1ra/G-MSCs/HA-sECM (test group), G-MSCs/HA-sECM (positive-control), scaling and root planing (SRP; negative control-1) or left untreated (no-treatment group; negative control 2). Differences in clinical attachment level (ΔCAL), probing depth (ΔPD), Gingival recession (ΔGR), radiographic defect volume (ΔRDV), and changes in bleeding on probing (BOP) between baseline and 16 weeks post-transplantation, as well as periodontal attachment level (PAL), junctional epithelium length (JE), connective tissue adhesion (CTA), cementum regeneration (CR) and bone regeneration (BR) at 16 weeks post-transplantation were evaluated. RESULTS: Isolated G-MSCs showed stem/progenitor cell characteristics. IL-1ra loaded and unloaded G-MSCs/HA-sECM showed higher ΔCAL, ΔPD, ΔGR, PAL, CR and BR as well as a lower JE compared to their negative controls and improved BOP. CONCLUSION: G-MSCs in conjunction with IL-1ra-loaded/unloaded HA-sECM show a significant periodontal regenerative potential.
Sebastian Paris - One of the best experts on this subject based on the ideXlab platform.
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Isolation and characterisation of human Gingival Margin-derived STRO-1/MACS + and MACS − cell populations
International Journal of Oral Science, 2015Co-Authors: Karim M Fawzy El-sayed, Sebastian Paris, Christian Graetz, Neemat Kassem, Mohamed Mekhemar, Hendrick Ungefroren, Fred Fändrich, Christof DörferAbstract:Recently, Gingival Margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo. As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS+) and STRO-1-negative (MACS−) cell populations from the human free Gingival Margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS+ and MACS− cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS+ and MACS− cell fractions showed plastic adherence. MACS+ cells, in contrast to MACS− cells, showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs (P
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isolation and characterisation of human Gingival Margin derived stro 1 macs and macs cell populations
International Journal of Oral Science, 2015Co-Authors: Sebastian Paris, Christian Graetz, Neemat Kassem, Mohamed Mekhemar, Hendrick Ungefroren, Fred Fändrich, Karim Fawzy M Elsayed, Christof DörferAbstract:Recently, Gingival Margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo. As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS+) and STRO-1-negative (MACS−) cell populations from the human free Gingival Margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS+ and MACS− cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS+ and MACS− cell fractions showed plastic adherence. MACS+ cells, in contrast to MACS− cells, showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs (P<0.01). More than 95% of MACS+ cells expressed CD105, CD90 and CD73; lacked the haematopoietic markers CD45, CD34 and CD14, and expressed STRO-1 and CD146/MUC18. MACS− cells showed a different surface marker expression profile, with almost no expression of CD14 or STRO-1, and more than 95% of these cells expressed CD73, CD90 and CD146/MUC18, as well as the haematopoietic markers CD34 and CD45 and CD105. MACS+ cells could be differentiated along osteoblastic, adipocytic and chondroblastic lineages. In contrast, MACS− cells demonstrated slight osteogenic potential. Unstimulated MACS+ cells showed significantly higher expression of collagen I (P<0.05) and collagen III (P<0.01), whereas MACS− cells demonstrated higher expression of osteonectin (P<0.05; Mann–Whitney). The present study is the first to compare Gingival MACS+ and MACS− cell populations demonstrating that MACS+ cells, in contrast to MACS− cells, harbour stem/progenitor cell characteristics. This study also validates the effectiveness of the STRO-1/MACS+ technique for the isolation of Gingival stem/progenitor cells. Human free Gingival Margin-derived STRO-1/MACS+ cells are a unique renewable source of multipotent stem/progenitor cells.
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Isolation and characterisation of human Gingival Margin-derived STRO-1/MACS^+ and MACS^− cell populations
International Journal of Oral Science, 2015Co-Authors: Karim M Fawzy El-sayed, Sebastian Paris, Christian Graetz, Neemat Kassem, Mohamed Mekhemar, Hendrick Ungefroren, Fred Fändrich, Christof DörferAbstract:Recently, Gingival Margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo . As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS^+) and STRO-1-negative (MACS^−) cell populations from the human free Gingival Margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS^+ and MACS^− cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS^+ and MACS^− cell fractions showed plastic adherence. MACS^+ cells, in contrast to MACS^− cells, showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs ( P
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periodontal regeneration employing Gingival Margin derived stem progenitor cells an animal study
Journal of Clinical Periodontology, 2012Co-Authors: Karim Fawzy M Elsayed, Sebastian Paris, Stephan T Becker, Matthias Neuschl, Wiebke De Buhr, Sonja Salzer, Andreas Wulff, Mahmoud I Elrefai, Mona S Darhous, Manal ElmasryAbstract:Aim This study investigated the periodontal regenerative potential of Gingival Margin-derived multipotent postnatal stem/progenitor cells. Material and Methods Periodontal defects were induced at six sites in eight miniature pigs in the premolar/molar area (−4 weeks). Autologous cells isolated from the Gingival Margin were magnetically sorted using STRO-1 antibodies and characterized flow cytometrically for the expression of CD14, CD31, CD34, CD45, CD117 and STRO-1 surface markers. Colony formation and multilineage differentiation potential were tested. The cells were expanded and loaded on deproteinized bovine cancellous bone (DBCB) and Collagen scaffolds. Within every miniature pig, six periodontal defects were randomly treated with loaded-DBCB (test group 1), unloaded-DBCB (control group 1), loaded-Collagen scaffolds (test group 2), unloaded-Collagen scaffolds (control group 1), scaling and root planing (negative control 1) or left untreated (negative control 2). Differences in clinical attachment level (ΔCAL), probing depth (ΔPD), Gingival recession (ΔGR) and radiographic defect volume (ΔRDV) between baseline and 12 weeks, as well as histological attachment level (HAL), junctional epithelium length (JE) and connective tissue adhesion (CTA) after 12 weeks were evaluated. Results Isolated cells showed stem/progenitor cell characteristics. Cell-loaded scaffolds showed higher ΔCAL, ΔPD, ΔGR, HAL and lower JE and CTA compared with unloaded scaffolds and negative controls. The sort of scaffold had no significant influence on the measured outcomes. Conclusion Gingival Margin-derived stem/progenitor cells show significant periodontal regenerative potential.
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Periodontal regeneration employing Gingival Margin-derived stem/progenitor cells: an animal study.
Journal of Clinical Periodontology, 2012Co-Authors: Karim M Fawzy El-sayed, Sebastian Paris, Stephan T Becker, Matthias Neuschl, Wiebke De Buhr, Sonja Salzer, Andreas Wulff, Mona S Darhous, Mahmoud I. El-refai, Manal El-masryAbstract:Aim This study investigated the periodontal regenerative potential of Gingival Margin-derived multipotent postnatal stem/progenitor cells. Material and Methods Periodontal defects were induced at six sites in eight miniature pigs in the premolar/molar area (−4 weeks). Autologous cells isolated from the Gingival Margin were magnetically sorted using STRO-1 antibodies and characterized flow cytometrically for the expression of CD14, CD31, CD34, CD45, CD117 and STRO-1 surface markers. Colony formation and multilineage differentiation potential were tested. The cells were expanded and loaded on deproteinized bovine cancellous bone (DBCB) and Collagen scaffolds. Within every miniature pig, six periodontal defects were randomly treated with loaded-DBCB (test group 1), unloaded-DBCB (control group 1), loaded-Collagen scaffolds (test group 2), unloaded-Collagen scaffolds (control group 1), scaling and root planing (negative control 1) or left untreated (negative control 2). Differences in clinical attachment level (ΔCAL), probing depth (ΔPD), Gingival recession (ΔGR) and radiographic defect volume (ΔRDV) between baseline and 12 weeks, as well as histological attachment level (HAL), junctional epithelium length (JE) and connective tissue adhesion (CTA) after 12 weeks were evaluated. Results Isolated cells showed stem/progenitor cell characteristics. Cell-loaded scaffolds showed higher ΔCAL, ΔPD, ΔGR, HAL and lower JE and CTA compared with unloaded scaffolds and negative controls. The sort of scaffold had no significant influence on the measured outcomes. Conclusion Gingival Margin-derived stem/progenitor cells show significant periodontal regenerative potential.
Mohamed Mekhemar - One of the best experts on this subject based on the ideXlab platform.
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isolation and characterisation of human Gingival Margin derived stro 1 macs and macs cell populations
International Journal of Oral Science, 2015Co-Authors: Sebastian Paris, Christian Graetz, Neemat Kassem, Mohamed Mekhemar, Hendrick Ungefroren, Fred Fändrich, Karim Fawzy M Elsayed, Christof DörferAbstract:Recently, Gingival Margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo. As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS+) and STRO-1-negative (MACS−) cell populations from the human free Gingival Margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS+ and MACS− cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS+ and MACS− cell fractions showed plastic adherence. MACS+ cells, in contrast to MACS− cells, showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs (P<0.01). More than 95% of MACS+ cells expressed CD105, CD90 and CD73; lacked the haematopoietic markers CD45, CD34 and CD14, and expressed STRO-1 and CD146/MUC18. MACS− cells showed a different surface marker expression profile, with almost no expression of CD14 or STRO-1, and more than 95% of these cells expressed CD73, CD90 and CD146/MUC18, as well as the haematopoietic markers CD34 and CD45 and CD105. MACS+ cells could be differentiated along osteoblastic, adipocytic and chondroblastic lineages. In contrast, MACS− cells demonstrated slight osteogenic potential. Unstimulated MACS+ cells showed significantly higher expression of collagen I (P<0.05) and collagen III (P<0.01), whereas MACS− cells demonstrated higher expression of osteonectin (P<0.05; Mann–Whitney). The present study is the first to compare Gingival MACS+ and MACS− cell populations demonstrating that MACS+ cells, in contrast to MACS− cells, harbour stem/progenitor cell characteristics. This study also validates the effectiveness of the STRO-1/MACS+ technique for the isolation of Gingival stem/progenitor cells. Human free Gingival Margin-derived STRO-1/MACS+ cells are a unique renewable source of multipotent stem/progenitor cells.
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Isolation and characterisation of human Gingival Margin-derived STRO-1/MACS + and MACS − cell populations
International Journal of Oral Science, 2015Co-Authors: Karim M Fawzy El-sayed, Sebastian Paris, Christian Graetz, Neemat Kassem, Mohamed Mekhemar, Hendrick Ungefroren, Fred Fändrich, Christof DörferAbstract:Recently, Gingival Margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo. As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS+) and STRO-1-negative (MACS−) cell populations from the human free Gingival Margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS+ and MACS− cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS+ and MACS− cell fractions showed plastic adherence. MACS+ cells, in contrast to MACS− cells, showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs (P
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Isolation and characterisation of human Gingival Margin-derived STRO-1/MACS^+ and MACS^− cell populations
International Journal of Oral Science, 2015Co-Authors: Karim M Fawzy El-sayed, Sebastian Paris, Christian Graetz, Neemat Kassem, Mohamed Mekhemar, Hendrick Ungefroren, Fred Fändrich, Christof DörferAbstract:Recently, Gingival Margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo . As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS^+) and STRO-1-negative (MACS^−) cell populations from the human free Gingival Margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS^+ and MACS^− cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS^+ and MACS^− cell fractions showed plastic adherence. MACS^+ cells, in contrast to MACS^− cells, showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs ( P
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Periodontal regeneration employing Gingival Margin‐derived stem/progenitor cells in conjunction with IL‐1ra‐hydrogel synthetic extracellular matrix
Journal of Clinical Periodontology, 2015Co-Authors: Karim M Fawzy El-sayed, Mohamed Mekhemar, Stephan T Becker, Telse Bahr, Marwa Hegab, Jan Receveur, Carola Heneweer, Joerg Wiltfang, Benedicta E. Beck-broichsitter, Christof DörferAbstract:AIM: This study investigated the periodontal regenerative potential of Gingival Margin-derived stem/progenitor cells (G-MSCs) in conjunction with IL-1ra-releasing hyaluronic acid synthetic extracellular matrix (HA-sECM). MATERIALS AND METHODS: Periodontal defects were induced at four sites in eight miniature pigs in the premolar/molar area (-4 weeks). Autologus G-MSCs were isolated from the free Gingival Margin and magnetically sorted, using anti-STRO-1 antibodies. Colony formation and multilineage differentiation potential were tested. The G-MSCs were expanded and incorporated into IL-1ra-loaded/unloaded HA-sECM. Within every miniature pig, four periodontal defects were randomly treated with IL-1ra/G-MSCs/HA-sECM (test group), G-MSCs/HA-sECM (positive-control), scaling and root planing (SRP; negative control-1) or left untreated (no-treatment group; negative control 2). Differences in clinical attachment level (ΔCAL), probing depth (ΔPD), Gingival recession (ΔGR), radiographic defect volume (ΔRDV), and changes in bleeding on probing (BOP) between baseline and 16 weeks post-transplantation, as well as periodontal attachment level (PAL), junctional epithelium length (JE), connective tissue adhesion (CTA), cementum regeneration (CR) and bone regeneration (BR) at 16 weeks post-transplantation were evaluated. RESULTS: Isolated G-MSCs showed stem/progenitor cell characteristics. IL-1ra loaded and unloaded G-MSCs/HA-sECM showed higher ΔCAL, ΔPD, ΔGR, PAL, CR and BR as well as a lower JE compared to their negative controls and improved BOP. CONCLUSION: G-MSCs in conjunction with IL-1ra-loaded/unloaded HA-sECM show a significant periodontal regenerative potential.
Karim M Fawzy El-sayed - One of the best experts on this subject based on the ideXlab platform.
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Isolation and characterisation of human Gingival Margin-derived STRO-1/MACS + and MACS − cell populations
International Journal of Oral Science, 2015Co-Authors: Karim M Fawzy El-sayed, Sebastian Paris, Christian Graetz, Neemat Kassem, Mohamed Mekhemar, Hendrick Ungefroren, Fred Fändrich, Christof DörferAbstract:Recently, Gingival Margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo. As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS+) and STRO-1-negative (MACS−) cell populations from the human free Gingival Margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS+ and MACS− cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS+ and MACS− cell fractions showed plastic adherence. MACS+ cells, in contrast to MACS− cells, showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs (P
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Isolation and characterisation of human Gingival Margin-derived STRO-1/MACS^+ and MACS^− cell populations
International Journal of Oral Science, 2015Co-Authors: Karim M Fawzy El-sayed, Sebastian Paris, Christian Graetz, Neemat Kassem, Mohamed Mekhemar, Hendrick Ungefroren, Fred Fändrich, Christof DörferAbstract:Recently, Gingival Margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo . As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive (MACS^+) and STRO-1-negative (MACS^−) cell populations from the human free Gingival Margin. Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies. Subsequently, the MACS^+ and MACS^− cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. Mineralisation marker expression was examined using real-time polymerase chain reaction (PCR). MACS^+ and MACS^− cell fractions showed plastic adherence. MACS^+ cells, in contrast to MACS^− cells, showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs ( P
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Periodontal regeneration employing Gingival Margin‐derived stem/progenitor cells in conjunction with IL‐1ra‐hydrogel synthetic extracellular matrix
Journal of Clinical Periodontology, 2015Co-Authors: Karim M Fawzy El-sayed, Mohamed Mekhemar, Stephan T Becker, Telse Bahr, Marwa Hegab, Jan Receveur, Carola Heneweer, Joerg Wiltfang, Benedicta E. Beck-broichsitter, Christof DörferAbstract:AIM: This study investigated the periodontal regenerative potential of Gingival Margin-derived stem/progenitor cells (G-MSCs) in conjunction with IL-1ra-releasing hyaluronic acid synthetic extracellular matrix (HA-sECM). MATERIALS AND METHODS: Periodontal defects were induced at four sites in eight miniature pigs in the premolar/molar area (-4 weeks). Autologus G-MSCs were isolated from the free Gingival Margin and magnetically sorted, using anti-STRO-1 antibodies. Colony formation and multilineage differentiation potential were tested. The G-MSCs were expanded and incorporated into IL-1ra-loaded/unloaded HA-sECM. Within every miniature pig, four periodontal defects were randomly treated with IL-1ra/G-MSCs/HA-sECM (test group), G-MSCs/HA-sECM (positive-control), scaling and root planing (SRP; negative control-1) or left untreated (no-treatment group; negative control 2). Differences in clinical attachment level (ΔCAL), probing depth (ΔPD), Gingival recession (ΔGR), radiographic defect volume (ΔRDV), and changes in bleeding on probing (BOP) between baseline and 16 weeks post-transplantation, as well as periodontal attachment level (PAL), junctional epithelium length (JE), connective tissue adhesion (CTA), cementum regeneration (CR) and bone regeneration (BR) at 16 weeks post-transplantation were evaluated. RESULTS: Isolated G-MSCs showed stem/progenitor cell characteristics. IL-1ra loaded and unloaded G-MSCs/HA-sECM showed higher ΔCAL, ΔPD, ΔGR, PAL, CR and BR as well as a lower JE compared to their negative controls and improved BOP. CONCLUSION: G-MSCs in conjunction with IL-1ra-loaded/unloaded HA-sECM show a significant periodontal regenerative potential.
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Periodontal regeneration employing Gingival Margin-derived stem/progenitor cells: an animal study.
Journal of Clinical Periodontology, 2012Co-Authors: Karim M Fawzy El-sayed, Sebastian Paris, Stephan T Becker, Matthias Neuschl, Wiebke De Buhr, Sonja Salzer, Andreas Wulff, Mona S Darhous, Mahmoud I. El-refai, Manal El-masryAbstract:Aim This study investigated the periodontal regenerative potential of Gingival Margin-derived multipotent postnatal stem/progenitor cells. Material and Methods Periodontal defects were induced at six sites in eight miniature pigs in the premolar/molar area (−4 weeks). Autologous cells isolated from the Gingival Margin were magnetically sorted using STRO-1 antibodies and characterized flow cytometrically for the expression of CD14, CD31, CD34, CD45, CD117 and STRO-1 surface markers. Colony formation and multilineage differentiation potential were tested. The cells were expanded and loaded on deproteinized bovine cancellous bone (DBCB) and Collagen scaffolds. Within every miniature pig, six periodontal defects were randomly treated with loaded-DBCB (test group 1), unloaded-DBCB (control group 1), loaded-Collagen scaffolds (test group 2), unloaded-Collagen scaffolds (control group 1), scaling and root planing (negative control 1) or left untreated (negative control 2). Differences in clinical attachment level (ΔCAL), probing depth (ΔPD), Gingival recession (ΔGR) and radiographic defect volume (ΔRDV) between baseline and 12 weeks, as well as histological attachment level (HAL), junctional epithelium length (JE) and connective tissue adhesion (CTA) after 12 weeks were evaluated. Results Isolated cells showed stem/progenitor cell characteristics. Cell-loaded scaffolds showed higher ΔCAL, ΔPD, ΔGR, HAL and lower JE and CTA compared with unloaded scaffolds and negative controls. The sort of scaffold had no significant influence on the measured outcomes. Conclusion Gingival Margin-derived stem/progenitor cells show significant periodontal regenerative potential.