The Experts below are selected from a list of 51 Experts worldwide ranked by ideXlab platform
Reina Bendayan - One of the best experts on this subject based on the ideXlab platform.
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Expression of the ATP-binding Cassette Membrane Transporter, ABCG2, in Human and Rodent Brain Microvessel Endothelial and Glial Cell Culture Systems
Pharmaceutical Research, 2007Co-Authors: Karlo Babakhanian, Manisha Ramaswamy, Alexandre Prat, Karolina Wosik, Reina BendayanAbstract:Purpose The function of ABCG2 (BCRP), a member of the ATP-binding cassette (ABC) superfamily of membrane-associated drug transporters, at the blood-brain barrier remains highly controversial. This project investigates the functional expression of endogenous ABCG2 in Cultures of human and rodent brain Cellular compartments. Materials and Methods RT-PCR, western blot and fluorescent immunocytochemical analyses were performed on ABCG2-overexpressing human breast cancer (MCF-MX100) Cells, human and rat brain microvessel endothelial (HBEC and RBE4, respectively), and rat Glial Cells. Results RT-PCR analysis detected ABCG2 mRNA in all the Cell Culture systems. Western blot analysis with anti-ABCG2 monoclonal BXP-21 antibody detected a robust band at ∼72 kDa in the ABCG2-overexpressing MCF-MX100 Cell line, whereas low expression was found in human and rat brain Cell systems. Immunofluorescence microscopy detected predominant plasma membrane localization of ABCG2 in MCF-MX100 Cells but weak signal in all brain Cellular compartments. In the presence of ABCG2 inhibitors, the accumulation of ^3H-mitoxantrone and pheophorbide A, two established ABCG2 substrates, was significantly increased in MCF-MX100 Cells but not in the human and rodent brain Cell Culture systems. Conclusions Our data show low endogenous ABCG2 protein expression, localization and activity in Cultures of human and rat brain microvessel endothelial and Glial Cells.
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expression of the atp binding cassette membrane transporter abcg2 in human and rodent brain microvessel endothelial and Glial Cell Culture systems
Pharmaceutical Research, 2007Co-Authors: Karlo Babakhanian, Manisha Ramaswamy, Alexandre Prat, Karolina Wosik, Reina BendayanAbstract:Purpose The function of ABCG2 (BCRP), a member of the ATP-binding cassette (ABC) superfamily of membrane-associated drug transporters, at the blood-brain barrier remains highly controversial. This project investigates the functional expression of endogenous ABCG2 in Cultures of human and rodent brain Cellular compartments.
Karlo Babakhanian - One of the best experts on this subject based on the ideXlab platform.
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Expression of the ATP-binding Cassette Membrane Transporter, ABCG2, in Human and Rodent Brain Microvessel Endothelial and Glial Cell Culture Systems
Pharmaceutical Research, 2007Co-Authors: Karlo Babakhanian, Manisha Ramaswamy, Alexandre Prat, Karolina Wosik, Reina BendayanAbstract:Purpose The function of ABCG2 (BCRP), a member of the ATP-binding cassette (ABC) superfamily of membrane-associated drug transporters, at the blood-brain barrier remains highly controversial. This project investigates the functional expression of endogenous ABCG2 in Cultures of human and rodent brain Cellular compartments. Materials and Methods RT-PCR, western blot and fluorescent immunocytochemical analyses were performed on ABCG2-overexpressing human breast cancer (MCF-MX100) Cells, human and rat brain microvessel endothelial (HBEC and RBE4, respectively), and rat Glial Cells. Results RT-PCR analysis detected ABCG2 mRNA in all the Cell Culture systems. Western blot analysis with anti-ABCG2 monoclonal BXP-21 antibody detected a robust band at ∼72 kDa in the ABCG2-overexpressing MCF-MX100 Cell line, whereas low expression was found in human and rat brain Cell systems. Immunofluorescence microscopy detected predominant plasma membrane localization of ABCG2 in MCF-MX100 Cells but weak signal in all brain Cellular compartments. In the presence of ABCG2 inhibitors, the accumulation of ^3H-mitoxantrone and pheophorbide A, two established ABCG2 substrates, was significantly increased in MCF-MX100 Cells but not in the human and rodent brain Cell Culture systems. Conclusions Our data show low endogenous ABCG2 protein expression, localization and activity in Cultures of human and rat brain microvessel endothelial and Glial Cells.
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expression of the atp binding cassette membrane transporter abcg2 in human and rodent brain microvessel endothelial and Glial Cell Culture systems
Pharmaceutical Research, 2007Co-Authors: Karlo Babakhanian, Manisha Ramaswamy, Alexandre Prat, Karolina Wosik, Reina BendayanAbstract:Purpose The function of ABCG2 (BCRP), a member of the ATP-binding cassette (ABC) superfamily of membrane-associated drug transporters, at the blood-brain barrier remains highly controversial. This project investigates the functional expression of endogenous ABCG2 in Cultures of human and rodent brain Cellular compartments.
E. Obuchowicz - One of the best experts on this subject based on the ideXlab platform.
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Moclobemide exerts anti-inflammatory effect in lipopolysaccharide-activated primary mixed Glial Cell Culture
Naunyn-Schmiedeberg's Archives of Pharmacology, 2010Co-Authors: A. M. Bielecka, M. Paul-samojedny, E. ObuchowiczAbstract:An increasing body of evidence indicates that Glial activation and neuroinflammation play an important role in the pathogenesis of psychiatric and neurodegenerative diseases. Activated Glial Cells secrete various cytokines that influence neurotransmission, hypothalamus–pituitary–adrenal axis activity, neuronal plasticity and neurogenesis. It has been suggested that alterations in cytokine networks are involved in the mechanism of action of antidepressant drugs. Until now, only a few studies demonstrated that some tricyclic antidepressants and selective serotonin reuptake inhibitors reduced production of pro-inflammatory cytokines in brain glia Cells. We have investigated for the first time whether the antidepressant, moclobemide (a reversible selective inhibitor of monoamine oxidase-A) has an influence on pro-inflammatory cytokines [interleukin (IL)-1β and tumor necrosis factor (TNF)-α] and anti-inflammatory cytokine (IL-10) in primary rat mixed Glial Cell Cultures stimulated by lipopolysaccharide (LPS). Our results showed that moclobemide used in a wide range of concentrations diminished LPS-stimulated IL-1β and TNF-α mRNAs expression in Cellular extracts and remarkably reduced the levels of both pro-inflammatory cytokines in Culture medium. In opposite to this, the drug had no influence on IL-10 mRNA and slightly reduced IL-10 concentration. Moreover, moclobemide decreased LPS-stimulated translocation of NFκB p65 subunit into Cellular nuclei. These results suggest that moclobemide exerts anti-inflammatory effect in the central nervous system because it affects the balance between pro- and anti-inflammatory cytokines (IL-1β, TNF-α/IL-10) in primary mixed Glial Cell Cultures.
Manisha Ramaswamy - One of the best experts on this subject based on the ideXlab platform.
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Expression of the ATP-binding Cassette Membrane Transporter, ABCG2, in Human and Rodent Brain Microvessel Endothelial and Glial Cell Culture Systems
Pharmaceutical Research, 2007Co-Authors: Karlo Babakhanian, Manisha Ramaswamy, Alexandre Prat, Karolina Wosik, Reina BendayanAbstract:Purpose The function of ABCG2 (BCRP), a member of the ATP-binding cassette (ABC) superfamily of membrane-associated drug transporters, at the blood-brain barrier remains highly controversial. This project investigates the functional expression of endogenous ABCG2 in Cultures of human and rodent brain Cellular compartments. Materials and Methods RT-PCR, western blot and fluorescent immunocytochemical analyses were performed on ABCG2-overexpressing human breast cancer (MCF-MX100) Cells, human and rat brain microvessel endothelial (HBEC and RBE4, respectively), and rat Glial Cells. Results RT-PCR analysis detected ABCG2 mRNA in all the Cell Culture systems. Western blot analysis with anti-ABCG2 monoclonal BXP-21 antibody detected a robust band at ∼72 kDa in the ABCG2-overexpressing MCF-MX100 Cell line, whereas low expression was found in human and rat brain Cell systems. Immunofluorescence microscopy detected predominant plasma membrane localization of ABCG2 in MCF-MX100 Cells but weak signal in all brain Cellular compartments. In the presence of ABCG2 inhibitors, the accumulation of ^3H-mitoxantrone and pheophorbide A, two established ABCG2 substrates, was significantly increased in MCF-MX100 Cells but not in the human and rodent brain Cell Culture systems. Conclusions Our data show low endogenous ABCG2 protein expression, localization and activity in Cultures of human and rat brain microvessel endothelial and Glial Cells.
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expression of the atp binding cassette membrane transporter abcg2 in human and rodent brain microvessel endothelial and Glial Cell Culture systems
Pharmaceutical Research, 2007Co-Authors: Karlo Babakhanian, Manisha Ramaswamy, Alexandre Prat, Karolina Wosik, Reina BendayanAbstract:Purpose The function of ABCG2 (BCRP), a member of the ATP-binding cassette (ABC) superfamily of membrane-associated drug transporters, at the blood-brain barrier remains highly controversial. This project investigates the functional expression of endogenous ABCG2 in Cultures of human and rodent brain Cellular compartments.
Alexandre Prat - One of the best experts on this subject based on the ideXlab platform.
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Expression of the ATP-binding Cassette Membrane Transporter, ABCG2, in Human and Rodent Brain Microvessel Endothelial and Glial Cell Culture Systems
Pharmaceutical Research, 2007Co-Authors: Karlo Babakhanian, Manisha Ramaswamy, Alexandre Prat, Karolina Wosik, Reina BendayanAbstract:Purpose The function of ABCG2 (BCRP), a member of the ATP-binding cassette (ABC) superfamily of membrane-associated drug transporters, at the blood-brain barrier remains highly controversial. This project investigates the functional expression of endogenous ABCG2 in Cultures of human and rodent brain Cellular compartments. Materials and Methods RT-PCR, western blot and fluorescent immunocytochemical analyses were performed on ABCG2-overexpressing human breast cancer (MCF-MX100) Cells, human and rat brain microvessel endothelial (HBEC and RBE4, respectively), and rat Glial Cells. Results RT-PCR analysis detected ABCG2 mRNA in all the Cell Culture systems. Western blot analysis with anti-ABCG2 monoclonal BXP-21 antibody detected a robust band at ∼72 kDa in the ABCG2-overexpressing MCF-MX100 Cell line, whereas low expression was found in human and rat brain Cell systems. Immunofluorescence microscopy detected predominant plasma membrane localization of ABCG2 in MCF-MX100 Cells but weak signal in all brain Cellular compartments. In the presence of ABCG2 inhibitors, the accumulation of ^3H-mitoxantrone and pheophorbide A, two established ABCG2 substrates, was significantly increased in MCF-MX100 Cells but not in the human and rodent brain Cell Culture systems. Conclusions Our data show low endogenous ABCG2 protein expression, localization and activity in Cultures of human and rat brain microvessel endothelial and Glial Cells.
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expression of the atp binding cassette membrane transporter abcg2 in human and rodent brain microvessel endothelial and Glial Cell Culture systems
Pharmaceutical Research, 2007Co-Authors: Karlo Babakhanian, Manisha Ramaswamy, Alexandre Prat, Karolina Wosik, Reina BendayanAbstract:Purpose The function of ABCG2 (BCRP), a member of the ATP-binding cassette (ABC) superfamily of membrane-associated drug transporters, at the blood-brain barrier remains highly controversial. This project investigates the functional expression of endogenous ABCG2 in Cultures of human and rodent brain Cellular compartments.