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Ilse Cleenwerck - One of the best experts on this subject based on the ideXlab platform.
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Gluconacetobacter dulcium sp nov a novel Gluconacetobacter species from sugar rich environments
International Journal of Systematic and Evolutionary Microbiology, 2020Co-Authors: Atena Sadat Sombolestani, Ilse Cleenwerck, Luc De Vuyst, Margo Cnockaert, Wim Borremans, Anneleen D Wieme, Yvan Moutia, Stijn Spaepen, Peter VandammeAbstract:A phylogenomic analysis based on 107 single-copy core genes revealed that three strains from sugar-rich environments, i.e. LMG 1728T, LMG 1731 and LMG 22058, represented a single, novel Gluconacetobacter lineage with Gluconacetobacter liquefaciens as nearest validly named neighbour. OrthoANIu and digital DNA-DNA hybridization analyses among these strains and Gluconacetobacter type strains confirmed that the three strains represented a novel Gluconacetobacter species. Biochemical characteristics and MALDI-TOF mass spectra allowed differentiation of this novel species from the type strains of G. liquefaciens and other closely related Gluconacetobacter species. We therefore propose to classify strains LMG 1728T, LMG 1731 and LMG 22058 in the novel species Gluconacetobacter dulcium sp. nov., with LMG 1728T (=CECT 30142T) as the type strain.
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Gluconacetobacter medellinensis sp nov cellulose and non cellulose producing acetic acid bacteria isolated from vinegar
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Cristina Castro, Ilse Cleenwerck, Janja Trcek, Robin Zuluaga, Paul De Vos, Gloria Caro, Ricardo Aguirre, Jeanluc Putaux, Piedad GananAbstract:The phylogenetic position of a cellulose-producing acetic acid bacterium, strain ID13488, isolated from commercially available Colombian homemade fruit vinegar, was investigated. Analyses using nearly complete 16S rRNA gene sequences, nearly complete 16S-23S rRNA gene internal transcribed spacer (ITS) sequences, as well as concatenated partial sequences of the housekeeping genes dnaK, groEL and rpoB, allocated the micro-organism to the genus Gluconacetobacter, and more precisely to the Gluconacetobacter xylinus group. Moreover, the data suggested that the micro-organism belongs to a novel species in this genus, together with LMG 1693(T), a non-cellulose-producing strain isolated from vinegar by Kondo and previously classified as a strain of Gluconacetobacter xylinus. DNA-DNA hybridizations confirmed this finding, revealing a DNA-DNA relatedness value of 81 % between strains ID13488 and LMG 1693(T), and values <70 % between strain LMG 1693(T) and the type strains of the closest phylogenetic neighbours. Additionally, the classification of strains ID13488 and LMG 1693(T) into a single novel species was supported by amplified fragment length polymorphism (AFLP) and (GTG)5-PCR DNA fingerprinting data, as well as by phenotypic data. Strains ID13488 and LMG 1693(T) could be differentiated from closely related species of the genus Gluconacetobacter by their ability to produce 2- and 5-keto-d-gluconic acid from d-glucose, their ability to produce acid from sucrose, but not from 1-propanol, and their ability to grow on 3 % ethanol in the absence of acetic acid and on ethanol, d-ribose, d-xylose, sucrose, sorbitol, d-mannitol and d-gluconate as carbon sources. The DNA G+C content of strains ID13488 and LMG 1693(T) was 58.0 and 60.7 mol%, respectively. The major ubiquinone of LMG 1693(T) was Q-10. Taken together these data indicate that strains ID13488 and LMG 1693(T) represent a novel species of the genus Gluconacetobacter for which the name Gluconacetobacter medellinensis sp. nov. is proposed. The type strain is LMG 1693(T) ( = NBRC 3288(T) = Kondo 51(T)).
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Gluconacetobacter maltaceti sp. nov., a novel vinegar producing acetic acid bacterium.
Systematic and applied microbiology, 2012Co-Authors: Nina Slapšak, Ilse Cleenwerck, Paul De Vos, Janja TrcekAbstract:Abstract Comparison of HaeIII- and HpaII-restriction profiles of PCR-amplified 16S-23S rDNA ITS regions of Gluconacetobacter sp. LMG 1529T and SKU 1109 with restriction profiles of reference strains of acetic acid bacteria described by Trcek and Teuber [34] revealed the same but unique restriction profiles for LMG 1529T and SKU 1109. Further analyses of nearly complete 16S rRNA gene sequences, nearly complete 16S-23S rDNA ITS sequences, as well as concatenated partial sequences of the housekeeping genes dnaK, groEL and rpoB, allocated both strains to a single phylogenetic cluster well separated from the other species of the genus Gluconacetobacter. DNA–DNA hybridizations confirmed their novel species identity by 73% DNA–DNA relatedness between both strains, and values below the species level ( d -gluconic acid from d -glucose, their ability to use d -mannitol, d -gluconate and glycerol as carbon source and form acid from d -fructose, and their ability to grow without acetic acid. The major fatty acid of LMG 1529T and SKU 1109 is C18:1ω7c (60.2–64.8%). The DNA G + C content of LMG 1529T and SKU 1109 is 62.5 and 63.3 mol% respectively. The name Gluconacetobacter maltaceti sp. nov. is proposed. The type strain is LMG 1529T (= NBRC 14815T = NCIMB 8752T).
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Gluconacetobacter medellinensis sp. nov., cellulose- and non-cellulose-producing acetic acid bacteria isolated from vinegar.
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Cristina Castro, Ilse Cleenwerck, Janja Trcek, Robin Zuluaga, Paul De Vos, Gloria Caro, Ricardo Aguirre, Jeanluc Putaux, Piedad GananAbstract:The phylogenetic position of a cellulose-producing acetic acid bacterium, strain ID13488, isolated from commercially available Colombian homemade fruit vinegar, was investigated. Analyses using nearly complete 16S rRNA gene sequences, nearly complete 16S–23S rRNA gene internal transcribed spacer (ITS) sequences, as well as concatenated partial sequences of the housekeeping genes dnaK, groEL and rpoB, allocated the micro-organism to the genus Gluconacetobacter, and more precisely to the Gluconacetobacter xylinus group. Moreover, the data suggested that the micro-organism belongs to a novel species in this genus, together with LMG 1693T, a non-cellulose-producing strain isolated from vinegar by Kondo and previously classified as a strain of Gluconacetobacter xylinus. DNA–DNA hybridizations confirmed this finding, revealing a DNA–DNA relatedness value of 81 % between strains ID13488 and LMG 1693T, and values
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Phylogeny and differentiation of species of the genus Gluconacetobacter and related taxa based on multilocus sequence analyses of housekeeping genes and reclassification of Acetobacter xylinus subsp. sucrofermentans as Gluconacetobacter sucrofermenta
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Ilse Cleenwerck, Luc De VuystAbstract:Three housekeeping genes (dnaK, groEL and rpoB) of strains belonging to the genus Gluconacetobacter (37 strains) or related taxa (38 strains) were sequenced. Reference strains of the 15 species of the genus Gluconacetobacter were included. Phylogenetic trees generated using these gene sequences confirmed the existence of two phylogenetic groups within the genus Gluconacetobacter. These groups clustered separately in trees constructed using concatenated sequences of the three genes, indicating that the genus Gluconacetobacter should not remain a single genus and should be split, as suggested previously. Multilocus sequence analysis (MLSA) of the three housekeeping genes also proved useful for species differentiation in the family Acetobacteraceae. It also suggested that Gluconacetobacter xylinus LMG 18788, better known as the type and only strain of Acetobacter xylinus subsp. sucrofermentans, represents a distinct species in the genus Gluconacetobacter, and is not a true G. xylinus strain. In previous studies, this strain showed less than 70 % DNA relatedness to the type strains of G. xylinus and Gluconacetobacter nataicola, the phylogenetically nearest relatives, and could be distinguished from them phenotypically. Additionally, AFLP and (GTG)5-PCR DNA fingerprinting data supported its reclassification within a distinct species. The name Gluconacetobacter sucrofermentans (Toyosaki et al. 1996) sp. nov., comb. nov. is proposed.
Tai Uchimura - One of the best experts on this subject based on the ideXlab platform.
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Gluconacetobacter kakiaceti sp. nov., an acetic acid bacterium isolated from a traditional Japanese fruit vinegar
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Takao Iino, Kazuo Komagata, Rei Suzuki, Naoto Tanaka, Yoshimasa Kosako, Moriya Ohkuma, Tai UchimuraAbstract:Two novel acetic acid bacteria, strains G5-1T and I5-1, were isolated from traditional kaki vinegar (produced from fruits of kaki, Diospyros kaki Thunb.), collected in Kumamoto Prefecture, Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains G5-1T and I5-1 formed a distinct subline in the genus Gluconacetobacter and were closely related to Gluconacetobacter swingsii DST GL01T (99.3 % 16S rRNA gene sequence similarity). The isolates showed 96–100 % DNA–DNA relatedness with each other, but <53 % DNA–DNA relatedness with closely related members of the genus Gluconacetobacter . The isolates could be distinguished from closely related members of the genus Gluconacetobacter by not producing 2- and 5-ketogluconic acids from glucose, producing cellulose, growing without acetic acid and with 30 % (w/v) d-glucose, and producing acid from sugars and alcohols. Furthermore, the genomic DNA G+C contents of strains G5-1T and I5-1 were a little higher than those of their closest phylogenetic neighbours. On the basis of the phenotypic characteristics and phylogenetic position, strains G5-1T and I5-1 are assigned to a novel species, for which the name Gluconacetobacter kakiaceti sp. nov. is proposed; the type strain is G5-1T ( = JCM 25156T = NRIC 0798T = LMG 26206T).
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Gluconacetobacter kakiaceti sp. nov., an acetic acid bacterium isolated from a traditional Japanese fruit vinegar.
International journal of systematic and evolutionary microbiology, 2011Co-Authors: Takao Iino, Kazuo Komagata, Rei Suzuki, Naoto Tanaka, Yoshimasa Kosako, Moriya Ohkuma, Tai UchimuraAbstract:Two novel acetic acid bacteria, strains G5-1(T) and I5-1, were isolated from traditional kaki vinegar (produced from fruits of kaki, Diospyros kaki Thunb.), collected in Kumamoto Prefecture, Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains G5-1(T) and I5-1 formed a distinct subline in the genus Gluconacetobacter and were closely related to Gluconacetobacter swingsii DST GL01(T) (99.3% 16S rRNA gene sequence similarity). The isolates showed 96-100% DNA-DNA relatedness with each other, but
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Reclassification of Gluconacetobacter hansenii strains and proposals of Gluconacetobacter saccharivorans sp. nov. and Gluconacetobacter nataicola sp. nov.
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Puspita Lisdiyanti, Tai Uchimura, Richard R. Navarro, Kazuo KomagataAbstract:Ten strains previously assigned to Acetobacter hansenii (=Gluconacetobacter hansenii), Acetobacter pasteurianus LMG 1584 and eight reference strains of the genus Gluconacetobacter were reclassified by 16S rRNA gene sequencing, DNA-DNA similarity, DNA base composition and phenotypic characteristics. The A. hansenii strains and A. pasteurianus LMG 1584 were included in the cluster of acetic acid bacteria (family Acetobacteraceae) by 16S rRNA gene sequences. Further, they were separated into seven distinct groups by DNA-DNA similarity. DNA-DNA similarity group I was identified as G. hansenii. DNA-DNA similarity group II was retained as Gluconacetobacter sp., because DNA-DNA similarity between the strain and Gluconacetobacter entanii LTH 4560(T) could not be determined. This was due to a lack of availability of the type strain from any source. DNA-DNA similarity group III was regarded as a novel species, for which the name Gluconacetobacter saccharivorans sp. nov. (type strain, LMG 1582(T)=NRIC 0614(T)) is proposed. DNA-DNA similarity group IV included the type strains of Gluconacetobacter oboediens and Gluconacetobacter intermedius, and three A. hansenii strains. This group was identified as G. oboediens because high values of DNA-DNA similarity were obtained between the type strains and G. oboediens has priority over G. intermedius. DNA-DNA similarity group V was identified as Gluconacetobacter europaeus. DNA-DNA similarity group VI was regarded as a novel species, for which the name Gluconacetobacter nataicola sp. nov. (type strain, LMG 1536(T)=NRIC 0616(T)) is proposed. DNA-DNA similarity group VII was reclassified as Gluconacetobacter xylinus. The description of G. hansenii is emended.
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Kozakia baliensis gen. nov., sp. nov., a novel acetic acid bacterium in the alpha-proteobacteria.
International Journal of Systematic and Evolutionary Microbiology, 2002Co-Authors: Puspita Lisdiyanti, Susono Saono, Tai Uchimura, Hiroko Kawasaki, Yuzo Yamada, Tatsuji Seki, Yantyati Widyastuti, Kazuo KomagataAbstract:Four bacterial strains were isolated from palm brown sugar and ragi collected in Bali and Yogyakarta, Indonesia, by an enrichment culture approach for acetic acid bacteria. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four isolates constituted a cluster separate from the genera Acetobacter, Gluconobacter, Acidomonas, Gluconacetobacter and Asaia with a high bootstrap value in a phylogenetic tree. The isolates had high values of DNA-DNA similarity (78-100%) between one another and low values of the similarity (7-25%) to the type strains of Acetobacter aceti, Gluconobacter oxydans, Gluconacetobacter liquefaciens and Asaia bogorensis. The DNA base composition of the isolates ranged from 56.8 to 57.2 mol% G+C with a range of 0-4 mol%. The major quinone was Q-10. The isolates oxidized acetate and lactate to carbon dioxide and water, but the activity was weak, as with strains of Asaia bogorensis. The isolates differed from Asaia bogorensis strains in phenotypic characteristics. The name Kozakia baliensis gen. nov., sp. nov., is proposed for the four isolates. Strain Yo-3T (= NRIC 0488T = JCM 11301T = IFO 16664T = DSM 14400T) was isolated from palm brown sugar collected in Bali, Indonesia, and was designated as the type strain.
Kazuo Komagata - One of the best experts on this subject based on the ideXlab platform.
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Gluconacetobacter kakiaceti sp. nov., an acetic acid bacterium isolated from a traditional Japanese fruit vinegar
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Takao Iino, Kazuo Komagata, Rei Suzuki, Naoto Tanaka, Yoshimasa Kosako, Moriya Ohkuma, Tai UchimuraAbstract:Two novel acetic acid bacteria, strains G5-1T and I5-1, were isolated from traditional kaki vinegar (produced from fruits of kaki, Diospyros kaki Thunb.), collected in Kumamoto Prefecture, Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains G5-1T and I5-1 formed a distinct subline in the genus Gluconacetobacter and were closely related to Gluconacetobacter swingsii DST GL01T (99.3 % 16S rRNA gene sequence similarity). The isolates showed 96–100 % DNA–DNA relatedness with each other, but <53 % DNA–DNA relatedness with closely related members of the genus Gluconacetobacter . The isolates could be distinguished from closely related members of the genus Gluconacetobacter by not producing 2- and 5-ketogluconic acids from glucose, producing cellulose, growing without acetic acid and with 30 % (w/v) d-glucose, and producing acid from sugars and alcohols. Furthermore, the genomic DNA G+C contents of strains G5-1T and I5-1 were a little higher than those of their closest phylogenetic neighbours. On the basis of the phenotypic characteristics and phylogenetic position, strains G5-1T and I5-1 are assigned to a novel species, for which the name Gluconacetobacter kakiaceti sp. nov. is proposed; the type strain is G5-1T ( = JCM 25156T = NRIC 0798T = LMG 26206T).
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Gluconacetobacter kakiaceti sp. nov., an acetic acid bacterium isolated from a traditional Japanese fruit vinegar.
International journal of systematic and evolutionary microbiology, 2011Co-Authors: Takao Iino, Kazuo Komagata, Rei Suzuki, Naoto Tanaka, Yoshimasa Kosako, Moriya Ohkuma, Tai UchimuraAbstract:Two novel acetic acid bacteria, strains G5-1(T) and I5-1, were isolated from traditional kaki vinegar (produced from fruits of kaki, Diospyros kaki Thunb.), collected in Kumamoto Prefecture, Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains G5-1(T) and I5-1 formed a distinct subline in the genus Gluconacetobacter and were closely related to Gluconacetobacter swingsii DST GL01(T) (99.3% 16S rRNA gene sequence similarity). The isolates showed 96-100% DNA-DNA relatedness with each other, but
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Reclassification of Gluconacetobacter hansenii strains and proposals of Gluconacetobacter saccharivorans sp. nov. and Gluconacetobacter nataicola sp. nov.
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Puspita Lisdiyanti, Tai Uchimura, Richard R. Navarro, Kazuo KomagataAbstract:Ten strains previously assigned to Acetobacter hansenii (=Gluconacetobacter hansenii), Acetobacter pasteurianus LMG 1584 and eight reference strains of the genus Gluconacetobacter were reclassified by 16S rRNA gene sequencing, DNA-DNA similarity, DNA base composition and phenotypic characteristics. The A. hansenii strains and A. pasteurianus LMG 1584 were included in the cluster of acetic acid bacteria (family Acetobacteraceae) by 16S rRNA gene sequences. Further, they were separated into seven distinct groups by DNA-DNA similarity. DNA-DNA similarity group I was identified as G. hansenii. DNA-DNA similarity group II was retained as Gluconacetobacter sp., because DNA-DNA similarity between the strain and Gluconacetobacter entanii LTH 4560(T) could not be determined. This was due to a lack of availability of the type strain from any source. DNA-DNA similarity group III was regarded as a novel species, for which the name Gluconacetobacter saccharivorans sp. nov. (type strain, LMG 1582(T)=NRIC 0614(T)) is proposed. DNA-DNA similarity group IV included the type strains of Gluconacetobacter oboediens and Gluconacetobacter intermedius, and three A. hansenii strains. This group was identified as G. oboediens because high values of DNA-DNA similarity were obtained between the type strains and G. oboediens has priority over G. intermedius. DNA-DNA similarity group V was identified as Gluconacetobacter europaeus. DNA-DNA similarity group VI was regarded as a novel species, for which the name Gluconacetobacter nataicola sp. nov. (type strain, LMG 1536(T)=NRIC 0616(T)) is proposed. DNA-DNA similarity group VII was reclassified as Gluconacetobacter xylinus. The description of G. hansenii is emended.
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Kozakia baliensis gen. nov., sp. nov., a novel acetic acid bacterium in the alpha-proteobacteria.
International Journal of Systematic and Evolutionary Microbiology, 2002Co-Authors: Puspita Lisdiyanti, Susono Saono, Tai Uchimura, Hiroko Kawasaki, Yuzo Yamada, Tatsuji Seki, Yantyati Widyastuti, Kazuo KomagataAbstract:Four bacterial strains were isolated from palm brown sugar and ragi collected in Bali and Yogyakarta, Indonesia, by an enrichment culture approach for acetic acid bacteria. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four isolates constituted a cluster separate from the genera Acetobacter, Gluconobacter, Acidomonas, Gluconacetobacter and Asaia with a high bootstrap value in a phylogenetic tree. The isolates had high values of DNA-DNA similarity (78-100%) between one another and low values of the similarity (7-25%) to the type strains of Acetobacter aceti, Gluconobacter oxydans, Gluconacetobacter liquefaciens and Asaia bogorensis. The DNA base composition of the isolates ranged from 56.8 to 57.2 mol% G+C with a range of 0-4 mol%. The major quinone was Q-10. The isolates oxidized acetate and lactate to carbon dioxide and water, but the activity was weak, as with strains of Asaia bogorensis. The isolates differed from Asaia bogorensis strains in phenotypic characteristics. The name Kozakia baliensis gen. nov., sp. nov., is proposed for the four isolates. Strain Yo-3T (= NRIC 0488T = JCM 11301T = IFO 16664T = DSM 14400T) was isolated from palm brown sugar collected in Bali, Indonesia, and was designated as the type strain.
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Differentiation of Gluconacetobacter liquefaciens and Gluconacetobacter xylinus on the basis of DNA base composition, DNA relatedness, and oxidation products from glucose.
The Journal of general and applied microbiology, 1999Co-Authors: Richard R. Navarro, Kazuo KomagataAbstract:Gluconacetobacter liquefaciens and Gluconacetobacter xylinus share very similar phenotypic characteristics. They are differentiated by the production of a reddish-brown water-soluble pigment of the former and cellulose production of the latter. However, the loss of the two distinguishing features questions the separate standings of the two species. The DNA base composition and the DNA relatedness of strains of the two species, including other established species of acetic acid bacteria, were determined. G. liquefaciens strains had the higher guanine-plus-cytosine content (G+C content) in DNA, ranging from 63.5 to 66.9 mol%, and G. xylinus had the lower range, from 59.4 to 63.2 mol%. DNA hybridization revealed a low level of DNA similarity between the two species. G. liquefaciens strains produced 2,5-diketogluconic acid and pyrones from glucose, and G. xylinus strains produced 5-ketogluconic acid. From these results, it is unequivocal that G. liquefaciens is a distinct species from G. xylinus.
Lindsay I. Sly - One of the best experts on this subject based on the ideXlab platform.
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Design, development, and use of molecular primers and probes for the detection of Gluconacetobacter species in the pink sugarcane mealybug.
Microbial ecology, 2005Co-Authors: I. H. Franke-whittle, Michael G. O'shea, Graham J. Leonard, Lindsay I. SlyAbstract:Molecular tools for the species-specific detection of Gluconacetobacter sacchari, Gluconacetobacter diazotrophicus, and Gluconacetobacter liquefaciens from the pink sugarcane mealybug (PSMB) Saccharicoccus sacchari Cockerell (Homiptera: Pseudococcidae) were developed and used in polymerase chain reactions (PCR) and in fluorescence in situ hybridizations (FISH) to better understand the microbial diversity and the numerical significance of the acetic acid bacteria in the PSMB microenvironment. The presence of these species in the PSMB occurred over a wide range of sites, but not in all sites in sugarcane-growing areas of Queensland, Australia, and was variable over time. Molecular probes for use in FISH were also designed for the three acetic acid bacterial species, and shown to be specific only for the target species. Use of these probes in FISH of “squashed” whole mealybugs indicated that these acetic acid bacteria species represent only a small proportion of the microbial population of the PSMB. Despite the detection of Glac. sacchari, Glac. diazotrophicus, and Glac. liquefaciens by PCR from different mealybugs isolated at various times and from various sugarcane-growing areas in Queensland, Australia, these bacteria do not appear to be significant commensals in the PSMB environment.
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Molecular investigation of the microbial populations of the pink sugarcane mealybug, Saccharicoccus sacchari
Annals of Microbiology, 2004Co-Authors: I. H. Franke-whittle, Michael G. O'shea, Graham J. Leonard, Lindsay I. SlyAbstract:In an attempt to better understand the microbial diversity and endosymbiotic microbiota of the pink sugarcane mealybug (PSMB) Saccharicoccus sacchari Cockerell (Homoptera: Pseudococcidae), culture-independent approaches, namely PCR, a 16S rDNA clone library, and temperature gradient gel electrophoresis (TGGE) were used. Previous work has indicated that the acetic acid bacteria Gluconacetobacter sacchari, Gluconacetobacter diazotrophicus, and Gluconacetobacter liquefaciens represent only a small proportion of the microbial community of the PSMB. These findings were supported in this study by TGGE, where no bands representing G. sacchari, G. diazotrophicus, and G. liquefaciens on the acrylamide gel could be observed following electrophoresis, and by a 16S rDNA clone library study, where no clones with the sequence of an acetic acid bacterium were found. Instead, TGGE revealed that the mealybug microbial community was dominated by beta- and gamma-Proteobacteria. The dominant band in TGGE gels found in a majority of the mealybug samples was most similar, according to BLAST analysis, to the beta-symbiont of the craw mealybug Antonina crawii and to Candidatus Tremblaya princeps, an endosymbiont from the mealybug Paracoccus nothofagicola. The sequences of other dominant bands were identified as gamma-Proteobacteria, and were most closely related to uncultured bacterial clones obtained from soil samples. Mealybugs collected from different areas in Queensland, Australia, were found to produce similar TGGE profiles, although there were a few exceptions. A 16S rDNA clone library based on DNA extracted from a mealybug collected from sugarcane in the Burdekin region in Queensland, Australia, indicated very low levels of diversity among mealybug microbial populations. All sequenced clones were most closely related to the same members of the gamma-Proteobacteria, according to BLAST analysis.
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DESCRIPTION OF Gluconacetobacter SACCHARI SP. NOV., A NEW SPECIES OF ACETIC ACID BACTERIUM ISOLATED FROM THE LEAF SHEATH OF SUGAR CANE AND FROM THE PINK SUGAR-CANE MEALY BUG
International Journal of Systematic and Evolutionary Microbiology, 1999Co-Authors: Ingrid H. Franke, Graham J. Leonard, Mark Fegan, Chris Hayward, Erko Stackebrandt, Lindsay I. SlyAbstract:A new species of the genus Gluconacetobacter, for which the name Gluconacetobacter sacchari sp. nov. is proposed, was isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bug, Saccharicoccus sacchari, found on sugar cane growing in Queensland and northern New South Wales, Australia. The nearest phylogenetic relatives in the α-subclass of the Proteobacteria are Gluconacetobacter liquefaciens and Gluconacetobacter diazotrophicus, which have 98·8–99·3% and 97·9–98·5% 16S rDNA sequence similarity, respectively, to members of Gluconacetobacter sacchari. On the basis of the phylogenetic positioning of the strains, DNA reassociation studies, phenotypic tests and the presence of the Q10 ubiquinone, this new species was assigned to the genus Gluconacetobacter. No single phenotypic characteristic is unique to the species, but the species can be differentiated phenotypically from closely related members of the acetic acid bacteria by growth in the presence of 0·01% malachite green, growth on 30% glucose, an inability to fix nitrogen and an inability to grow with the L-amino acids asparagine, glycine, glutamine, threonine and tryptophan when D-mannitol was supplied as the sole carbon and energy source. The type strain of this species is strain SRI 1794T (=DSM 12717T).
Marta Marzotto - One of the best experts on this subject based on the ideXlab platform.
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Description of Gluconacetobacter swingsii sp. nov. and Gluconacetobacter rhaeticus sp. nov., isolated from Italian apple fruit
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Franco Dellaglio, Danielle Janssens, Ilse Cleenwerck, Giovanna E. Felis, Katrien Engelbeen, Marta MarzottoAbstract:Two Gram-negative, rod-shaped, non-spore-forming bacteria (DST GL01T and DST GL02T) were isolated from apple fruit juice in the region of the Italian Alps. On the basis of 16S rRNA gene sequence similarities, strains DST GL01T and DST GL02T were shown to belong to the α-subclass of the Proteobacteria, and, in particular, to the genus Gluconacetobacter, in the Gluconacetobacter xylinus branch (98·5–100 %). Chemotaxonomic data (major ubiquinone, Q10; predominant fatty acid, C18 : 1ω7c , accounting for approximately 50 % of the fatty acid content) support the affiliation of both strains to the genus Gluconacetobacter. The results of DNA–DNA hybridizations, together with physiological and biochemical data, allowed genotypic and phenotypic differentiation between strains DST GL01T and DST GL02T and from the 11 validly published Gluconacetobacter species. They therefore represent two new species, for which the names Gluconacetobacter swingsii sp. nov. and Gluconacetobacter rhaeticus sp. nov. are proposed, with the type strains DST GL01T (=LMG 22125T=DSM 16373T) and DST GL02T (=LMG 22126T=DSM 16663T), respectively.
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Description of Gluconacetobacter swingsii sp. nov. and Gluconacetobacter rhaeticus sp. nov., isolated from Italian apple fruit.
International journal of systematic and evolutionary microbiology, 2005Co-Authors: Franco Dellaglio, Danielle Janssens, Ilse Cleenwerck, Giovanna E. Felis, Katrien Engelbeen, Marta MarzottoAbstract:Two Gram-negative, rod-shaped, non-spore-forming bacteria (DST GL01T and DST GL02T) were isolated from apple fruit juice in the region of the Italian Alps. On the basis of 16S rRNA gene sequence similarities, strains DST GL01T and DST GL02T were shown to belong to the alpha-subclass of the Proteobacteria, and, in particular, to the genus Gluconacetobacter, in the Gluconacetobacter xylinus branch (98.5-100 %). Chemotaxonomic data (major ubiquinone, Q10; predominant fatty acid, C(18 : 1omega7c), accounting for approximately 50 % of the fatty acid content) support the affiliation of both strains to the genus Gluconacetobacter. The results of DNA-DNA hybridizations, together with physiological and biochemical data, allowed genotypic and phenotypic differentiation between strains DST GL01T and DST GL02T and from the 11 validly published Gluconacetobacter species. They therefore represent two new species, for which the names Gluconacetobacter swingsii sp. nov. and Gluconacetobacter rhaeticus sp. nov. are proposed, with the type strains DST GL01T (=LMG 22125T=DSM 16373T) and DST GL02T (=LMG 22126T=DSM 16663T), respectively.