The Experts below are selected from a list of 7887 Experts worldwide ranked by ideXlab platform

Marjorie J. Mcshane - One of the best experts on this subject based on the ideXlab platform.

  • "Smart tattoo" Glucose Biosensors and effect of coencapsulated anti-inflammatory agents
    Journal of Diabetes Science and Technology, 2011
    Co-Authors: Rohit Srivastava, Ayesha Chaudhary, Rahul Dev Jayant, Marjorie J. Mcshane
    Abstract:

    Minimally invasive Glucose Biosensors with increased functional longevity form one of the most promising techniques for continuous Glucose monitoring. In the present study, we developed a novel nanoengineered microsphere formulation comprising alginate microsphere Glucose sensors and anti-inflammatory-drug-loaded alginate microspheres.

  • smart tattoo Glucose Biosensors and effect of coencapsulated anti inflammatory agents
    Journal of diabetes science and technology, 2011
    Co-Authors: Rohit Srivastava, Ayesha Chaudhary, Rahul Dev Jayant, Marjorie J. Mcshane
    Abstract:

    Background: Minimally invasive Glucose Biosensors with increased functional longevity form one of the most promising techniques for continuous Glucose monitoring. In the present study, we developed a novel nanoengineered microsphere formulation comprising alginate microsphere Glucose sensors and anti-inflammatory-drug-loaded alginate microspheres. Methods: The formulation was prepared and characterized for size, shape, in vitro drug release, biocompatibility, and in vivo acceptability. Glucose oxidase (GOx)- and Apo-GOx-based Glucose sensors were prepared and characterized. Sensing was performed both in distilled water and simulated interstitial body fluid. Layer -by-layer self-assembly techniques were used for preventing drug and sensing chemistry release. Finally, in vivo studies, involving histopathologic examination of subcutaneous tissue surrounding the implanted sensors using Sprague–Dawley rats, were performed to test the suppression of inflammation and fibrosis associated with Glucose sensor implantation. Results: The drug formulation showed 100% drug release with in 30 days with zero-order release kinetics. The GOx-based sensors showed good enzyme retention and enzyme activity over a period of 1 month. Apo-GOx-based visible and near-infrared sensors showed good sensitivity and analytical response range of 0–50 mM Glucose, with linear range up to 12 mM Glucose concentration. In vitro cell line studies proved biocompatibility of the material used. Finally, both anti-inflammatory drugs were successful in controlling the implant–tissue interface by suppressing inflammation at the implant site. Conclusion: The incorporation of anti-inflammatory drug with Glucose Biosensors shows promise in improving sensor biocompatibility, thereby suggesting potential application of alginate microspheres as “smart tattoo” Glucose sensors with increased functional longevity.

Joseph Wang - One of the best experts on this subject based on the ideXlab platform.

  • zwitterionic poly carboxybetaine hydrogels for Glucose Biosensors in complex media
    Biosensors and Bioelectronics, 2011
    Co-Authors: Wei Yang, Joseph Wang, Louisa R Carr, Shaoyi Jiang
    Abstract:

    Zwitterionic hydrogels based on poly(carboxybetaine) methacrylate (polyCBMA) were developed to protect implantable electrochemical Glucose Biosensors from biofouling in complex media. To enhance the linearity and sensitivity of the sensing profile, both physical and chemical adsorption methods were developed. Results show that Glucose sensors coated with polyCBMA hydrogels via the chemical method achieve very high sensitivity and good linearity in response to Glucose in PBS, 10%, 50%, and 100% human blood serum. Essentially identical Glucose signals were observed even after prolonged exposure to blood samples for over 12 days. The excellent performance of polyCBMA hydrogel coating offers great promise for designing biocompatible implantable Glucose Biosensors in biological medium.

  • thermally stable improved first generation Glucose Biosensors based on nafion Glucose oxidase modified heated electrodes
    Electrochemistry Communications, 2009
    Co-Authors: Tafeng Tseng, Yangli Yang, Minchieh Chuang, Michal Galik, Gerduwe Flechsig, Joseph Wang
    Abstract:

    We illustrate how the use of heated electrodes enhances the performance of Glucose Biosensors based on amperometric detection of the Glucose-oxidase generated hydrogen peroxide. Nafion is shown to be an excellent matrix to protect Glucose-oxidase from thermal inactivation during the heating pulses. The influence of the electrode temperature upon the amperometric response is examined. Temperature pulse amperometry (TPA) has been used to obtain convenient peak-shaped analytical signals. Surprisingly, up to 67.5 °C, the activity of Nafion-entrapped Glucose-oxidase is greatly enhanced (24-fold) by accelerated kinetics rather than decreased by thermal inactivation. Amperometric signals even at elevated temperatures are stable upon prolonged operation involving repetitive measurements. The linear calibration range is significantly extended.

  • electrochemical Glucose Biosensors
    Chemical Reviews, 2008
    Co-Authors: Joseph Wang
    Abstract:

    First-generation Glucose Biosensors relied on the use of the natural oxygen cosubstrate and the production and detection of hydrogen peroxide and were much simpler, especially when miniaturized sensors are concerned. More sophisticated bioelectronic systems for enhancing the electrical response, based on patterned monolayer or multilayer assemblies and organized enzyme networks on solid electrodes, have been developed for contacting GOx with the electrode support. Electrochemical Biosensors are well suited for satisfying the needs of personal (home) Glucose testing, and the majority of personal blood Glucose meters are based on disposable (screen-printed) enzyme electrode test strips, which are mass produced by the thick film (screen-printing) microfabrication technology. In the counter and an additional “baseline” working electrode, various membranes (mesh) are incorporated into the test strips along with surfactants, to provide a uniform sample coverage. Such devices offer considerable promise for obtaining the desired clinical information in a simpler, user-friendly, faster, and cheaper manner compared to traditional assays. Continuous ex-vivo monitoring of blood Glucose was proposed in 1974 and the majority of Glucose sensors used for in-vivo applications are based on the GOx-catalyzed oxidation of Glucose by oxygen. The major factors that play a role in the development of clinically accurate in-vivo Glucose sensors include issues related to biocompatibility, miniaturization, long-term stability of the enzyme and transducer, oxygen deficit, short stabilization times, in-vivo calibration, baseline drift, safety, and convenience.

  • Electrochemical Glucose Biosensors
    Electrochemical Sensors Biosensors and their Biomedical Applications, 2008
    Co-Authors: Joseph Wang
    Abstract:

    First-generation Glucose Biosensors relied on the use of the natural oxygen cosubstrate and the production and detection of hydrogen peroxide and were much simpler, especially when miniaturized sensors are concerned. More sophisticated bioelectronic systems for enhancing the electrical response, based on patterned monolayer or multilayer assemblies and organized enzyme networks on solid electrodes, have been developed for contacting GOx with the electrode support. Electrochemical Biosensors are well suited for satisfying the needs of personal (home) Glucose testing, and the majority of personal blood Glucose meters are based on disposable (screen-printed) enzyme electrode test strips, which are mass produced by the thick film (screen-printing) microfabrication technology. In the counter and an additional "baseline" working electrode, various membranes (mesh) are incorporated into the test strips along with surfactants, to provide a uniform sample coverage. Such devices offer considerable promise for obtaining the desired clinical information in a simpler, user-friendly, faster, and cheaper manner compared to traditional assays. Continuous ex-vivo monitoring of blood Glucose was proposed in 1974 and the majority of Glucose sensors used for in-vivo applications are based on the GOx-catalyzed oxidation of Glucose by oxygen. The major factors that play a role in the development of clinically accurate in-vivo Glucose sensors include issues related to biocompatibility, miniaturization, long-term stability of the enzyme and transducer, oxygen deficit, short stabilization times, in-vivo calibration, baseline drift, safety, and convenience. © 2008 Elsevier Inc. All rights reserved.

  • Glucose Biosensors 40 years of advances and challenges
    Sensors Update, 2001
    Co-Authors: Joseph Wang
    Abstract:

    Forty years have passed since Clark and Lyons proposed the concept of Glucose enzyme electrodes. Excellent economic prospects and fascinating potential for basic research have led to many sensor designs and detection principles for the biosensing of Glucose. Indeed, the entire field of Biosensors can trace its origin to this Glucose enzyme electrode. This review examines the history of electrochemical Glucose Biosensors, discusses their current status and assesses future prospects in connection primarily to the control and management of diabetes.

Junwei Di - One of the best experts on this subject based on the ideXlab platform.

Rohit Srivastava - One of the best experts on this subject based on the ideXlab platform.

  • "Smart tattoo" Glucose Biosensors and effect of coencapsulated anti-inflammatory agents
    Journal of Diabetes Science and Technology, 2011
    Co-Authors: Rohit Srivastava, Ayesha Chaudhary, Rahul Dev Jayant, Marjorie J. Mcshane
    Abstract:

    Minimally invasive Glucose Biosensors with increased functional longevity form one of the most promising techniques for continuous Glucose monitoring. In the present study, we developed a novel nanoengineered microsphere formulation comprising alginate microsphere Glucose sensors and anti-inflammatory-drug-loaded alginate microspheres.

  • smart tattoo Glucose Biosensors and effect of coencapsulated anti inflammatory agents
    Journal of diabetes science and technology, 2011
    Co-Authors: Rohit Srivastava, Ayesha Chaudhary, Rahul Dev Jayant, Marjorie J. Mcshane
    Abstract:

    Background: Minimally invasive Glucose Biosensors with increased functional longevity form one of the most promising techniques for continuous Glucose monitoring. In the present study, we developed a novel nanoengineered microsphere formulation comprising alginate microsphere Glucose sensors and anti-inflammatory-drug-loaded alginate microspheres. Methods: The formulation was prepared and characterized for size, shape, in vitro drug release, biocompatibility, and in vivo acceptability. Glucose oxidase (GOx)- and Apo-GOx-based Glucose sensors were prepared and characterized. Sensing was performed both in distilled water and simulated interstitial body fluid. Layer -by-layer self-assembly techniques were used for preventing drug and sensing chemistry release. Finally, in vivo studies, involving histopathologic examination of subcutaneous tissue surrounding the implanted sensors using Sprague–Dawley rats, were performed to test the suppression of inflammation and fibrosis associated with Glucose sensor implantation. Results: The drug formulation showed 100% drug release with in 30 days with zero-order release kinetics. The GOx-based sensors showed good enzyme retention and enzyme activity over a period of 1 month. Apo-GOx-based visible and near-infrared sensors showed good sensitivity and analytical response range of 0–50 mM Glucose, with linear range up to 12 mM Glucose concentration. In vitro cell line studies proved biocompatibility of the material used. Finally, both anti-inflammatory drugs were successful in controlling the implant–tissue interface by suppressing inflammation at the implant site. Conclusion: The incorporation of anti-inflammatory drug with Glucose Biosensors shows promise in improving sensor biocompatibility, thereby suggesting potential application of alginate microspheres as “smart tattoo” Glucose sensors with increased functional longevity.

Ming Gu - One of the best experts on this subject based on the ideXlab platform.