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Hong-won Suh - One of the best experts on this subject based on the ideXlab platform.

  • the role of spinally located dopamine d 2 receptors in the regulation of the blood Glucose Level in mice
    Pharmacological Reports, 2020
    Co-Authors: Jaeseung Hong, Yun-beom Sim, Jinghui Feng, Jinkoo Lee, Heejung Lee, Jungseok Park, Hong-won Suh
    Abstract:

    The possible role of dopamine D2 receptors located in the spinal cord in the regulation of the blood Glucose Level have not been investigated before. In the present study, the effect of D2 receptor agonist and antagonist administered intrathecal (it) injection on the blood Glucose Level were examined in the Institute of Cancer Research (ICR) mice. We found that it injection with carmoxirole (D2 receptor agonist) caused an elevation of the blood Glucose Level in a dose-dependent manner. Carmoxirole-induced increase of the blood Glucose was significantly attenuated by L-741,626 (D2 receptor antagonist). Previously, we indicated that intrathecal (it) treatment with 0.1 μg/5 μl pertussis toxin (PTX, a Gi/Go inhibitor) produces a hypoglycemic effect in ICR in a long-term manner. In the present study, it pretreatment with PTX for 6 days almost abolished the hyperglycemic effect induced by carmoxirole. The plasma insulin Level was elevated by carmoxirole, and L-741,626 or PTX pretreatment reduced carmoxirole-induced increment of the insulin Level. In addition, the plasma corticosterone Level was increased by carmoxirole but it pretreatment with L-741,626 or PTX did not affect carmoxirole-induced increment of the corticosterone Level. Our results suggest that D2 receptors located in the spinal cord play an important role in the elevation of the blood Glucose Level. Spinally located inhibitory G-proteins appear to be involved in hyperglycemic effect induced by carmoxirole.

  • The modulatory roles of oxyntomodulin and glucagon-like peptide 1 administered spinally in the regulation of the blood Glucose Level
    Neurochemical Journal, 2017
    Co-Authors: Yun-beom Sim, Soo-hyun Park, Jae-ryeong Lee, Sung-su Kim, Jun-sub Jung, Naveen Sharma, Hong-won Suh
    Abstract:

    Oxyntomodulin is known as a regulator of the blood Glucose homeostasis and food intake. In the present study, the possible roles of oxyntomodulin receptor (GCGR/GLP-1) located in the spinal cord for the regulation of the blood Glucose Level was investigated in ICR mice. We found in the present study that intrathecal (i.t.) injection with oxyntomodulin (from 1 to 10 μg) or glucagon-like peptide-1 (GLP-1; from 1 to 10 μg) alone did not affect the blood Glucose Level. However, i.t. administration with oxyntomodulin, but not GLP-1, caused a reduction of the blood Glucose Level in D-Glucose-fed model. The oxyntomodulininduced reduction of the blood Glucose Level in D-Glucose-fed model is followed by reduction of insulin Level. Furthermore, the i.t. co-administration of oxyntomodulin with GLP-1 caused a profound up-regulation of the blood Glucose Level. The profound hyperglycemic effect shown by co-treatment with oxyntomodulin and GLP-1 caused a profound up-regulation of plasma insulin Level. Our results suggest that the activation of oxyntomodulin system present in the spinal cord may play significant modulatory roles in the reduction of the blood Glucose Level in D-Glucose-fed model. Furthermore, GLP-1 located in the spinal cord shows a synergistic interaction with oxyntomodulin for producing hyperglycemia.

  • effect of histamine receptors agonists or antagonists administered intracerebroventricularly and intrathecally on the blood Glucose Level in immobilization stress model
    Animal Cells and Systems, 2015
    Co-Authors: Naveen Sharma, Soo-hyun Park, Jae-ryeong Lee, Yun-beom Sim, Sung-su Kim, Jun-sub Jung, Hong-won Suh
    Abstract:

    Effects of histamine receptors agonists or antagonists administered centrally (supraspinally or spinally) on immobilization stress-induced blood Glucose Levels were studied in Institute for Cancer Research mice. Mice were pretreated intracerebroventricularly (i.c.v.) or intrathecally (i.t.) with histamine (H1, H2, H3 and H4) receptor agonists or antagonists 10 min prior to immobilization stress (IMO) stimulation for 30 min. We found that the increased blood Glucose Level induced by IMO was attenuated by the i.c.v. pretreatment with α-methylhistamine (H3 agonist). However, H3 antagonist, H1, H2 and H4 receptor agonists and antagonists did not affect the blood Glucose Level. Also, i.t. pretreatment with α-methylhistamine or VUF 8430 attenuated the increased blood Glucose Level induced by IMO. In addition, cetirizine and carcinine enhanced the increased blood Glucose Level induced by IMO. However, the blood Glucose Level was not affected by H1 and H2 receptor agonists, H2 and H4 receptor antagonists. Cortico...

  • the modulatory role of spinally located histamine receptors in the regulation of the blood Glucose Level in d Glucose fed mice
    The Korean Journal of Physiology and Pharmacology, 2014
    Co-Authors: Yun-beom Sim, Soo-hyun Park, Sung-su Kim, Jun-sub Jung, Chea-ha Kim, Su-jin Kim, Ohk-hyun Ryu, Moon-gi Choi, Sumin Lim, Hong-won Suh
    Abstract:

    The possible roles of spinal histamine receptors in the regulation of the blood Glucose Level were studied in ICR mice. Mice were intrathecally (i.t.) treated with histamine 1 (H1) receptor agonist (2-pyridylethylamine) or antagonist (cetirizine), histamine 2 (H2) receptor agonist (dimaprit) or antagonist (ranitidine), histamine 3 (H3) receptor agonist (α-methylhistamine) or antagonist (carcinine) and histamine 4 (H4) receptor agonist (VUF 8430) or antagonist (JNJ 7777120), and the blood Glucose Level was measured at 30, 60 and 120 min after i.t. administration. The i.t. injection with α-methylhistamine, but not carcinine slightly caused an elevation of the blood Glucose Level. In addition, histamine H1, H2, and H4 receptor agonists and antagonists did not affect the blood Glucose Level. In D-Glucose-fed model, i.t. pretreatment with cetirizine enhanced the blood Glucose Level, whereas 2-pyridylethylamine did not affect. The i.t. pretreatment with dimaprit, but not ranitidine, enhanced the blood Glucose Level in D-Glucose-fed model. In addition, α-methylhistamine, but not carcinine, slightly but significantly enhanced the blood Glucose Level D-Glucose-fed model. Finally, i.t. pretreatment with JNJ 7777120, but not VUF 8430, slightly but significantly increased the blood Glucose Level. Although histamine receptors themselves located at the spinal cord do not exert any effect on the regulation of the blood Glucose Level, our results suggest that the activation of spinal histamine H2 receptors and the blockade of spinal histamine H1 or H3 receptors may play modulatory roles for up-regulation and down-regulation, respectively, of the blood Glucose Level in D-Glucose fed model.

  • Effect of GABA Receptor Agonists or Antagonists Injected Spinally on the Blood Glucose Level in Mice
    Neurochemical research, 2013
    Co-Authors: Yun-beom Sim, Soo-hyun Park, Sung-su Kim, Jun-sub Jung, Yu-jung Kang, Chea-ha Kim, Su-jin Kim, Ohk-hyun Ryu, Moon-gi Choi, Hong-won Suh
    Abstract:

    The possible roles of gamma-amino butyric acid (GABA) receptors located in the spinal cord for the regulation of the blood Glucose Level were studied in ICR mice. We found in the present study that intrathecal (i.t.) injection with baclofen (a GABAB receptor agonist; 1-10 μg/5 μl) or bicuculline (a GABAA receptor antagonist; 1-10 μg/5 μl) caused an elevation of the blood Glucose Level in a dose-dependent manner. The hyperglycemic effect induced by baclofen was more pronounced than that induced by bicuculline. However, muscimol (a GABAA receptor agonist; 1-5 μg/5 μl) or phaclofen (a GABAB receptor antagonist; 5-10 μg/5 μl) administered i.t. did not affect the blood Glucose Level. Baclofen-induced elevation of the blood Glucose was dose-dependently attenuated by phaclofen. Furthermore, i.t. pretreatment with pertussis toxin (PTX; 0.05 or 0.1 μg/5 μl) for 6 days dose-dependently reduced the hyperglycemic effect induced by baclofen. Our results suggest that GABAB receptors located in the spinal cord play important roles for the elevation of the blood Glucose Level. Spinally located PTX-sensitive G-proteins appear to be involved in hyperglycemic effect induced by baclofen. Furthermore, inactivation of GABAA receptors located in the spinal cord appears to be responsible for tonic up-regulation of the blood Glucose Level.

Jun-sub Jung - One of the best experts on this subject based on the ideXlab platform.

  • The modulatory roles of oxyntomodulin and glucagon-like peptide 1 administered spinally in the regulation of the blood Glucose Level
    Neurochemical Journal, 2017
    Co-Authors: Yun-beom Sim, Soo-hyun Park, Jae-ryeong Lee, Sung-su Kim, Jun-sub Jung, Naveen Sharma, Hong-won Suh
    Abstract:

    Oxyntomodulin is known as a regulator of the blood Glucose homeostasis and food intake. In the present study, the possible roles of oxyntomodulin receptor (GCGR/GLP-1) located in the spinal cord for the regulation of the blood Glucose Level was investigated in ICR mice. We found in the present study that intrathecal (i.t.) injection with oxyntomodulin (from 1 to 10 μg) or glucagon-like peptide-1 (GLP-1; from 1 to 10 μg) alone did not affect the blood Glucose Level. However, i.t. administration with oxyntomodulin, but not GLP-1, caused a reduction of the blood Glucose Level in D-Glucose-fed model. The oxyntomodulininduced reduction of the blood Glucose Level in D-Glucose-fed model is followed by reduction of insulin Level. Furthermore, the i.t. co-administration of oxyntomodulin with GLP-1 caused a profound up-regulation of the blood Glucose Level. The profound hyperglycemic effect shown by co-treatment with oxyntomodulin and GLP-1 caused a profound up-regulation of plasma insulin Level. Our results suggest that the activation of oxyntomodulin system present in the spinal cord may play significant modulatory roles in the reduction of the blood Glucose Level in D-Glucose-fed model. Furthermore, GLP-1 located in the spinal cord shows a synergistic interaction with oxyntomodulin for producing hyperglycemia.

  • effect of histamine receptors agonists or antagonists administered intracerebroventricularly and intrathecally on the blood Glucose Level in immobilization stress model
    Animal Cells and Systems, 2015
    Co-Authors: Naveen Sharma, Soo-hyun Park, Jae-ryeong Lee, Yun-beom Sim, Sung-su Kim, Jun-sub Jung, Hong-won Suh
    Abstract:

    Effects of histamine receptors agonists or antagonists administered centrally (supraspinally or spinally) on immobilization stress-induced blood Glucose Levels were studied in Institute for Cancer Research mice. Mice were pretreated intracerebroventricularly (i.c.v.) or intrathecally (i.t.) with histamine (H1, H2, H3 and H4) receptor agonists or antagonists 10 min prior to immobilization stress (IMO) stimulation for 30 min. We found that the increased blood Glucose Level induced by IMO was attenuated by the i.c.v. pretreatment with α-methylhistamine (H3 agonist). However, H3 antagonist, H1, H2 and H4 receptor agonists and antagonists did not affect the blood Glucose Level. Also, i.t. pretreatment with α-methylhistamine or VUF 8430 attenuated the increased blood Glucose Level induced by IMO. In addition, cetirizine and carcinine enhanced the increased blood Glucose Level induced by IMO. However, the blood Glucose Level was not affected by H1 and H2 receptor agonists, H2 and H4 receptor antagonists. Cortico...

  • the modulatory role of alpha melanocyte stimulating hormone administered spinally in the regulation of blood Glucose Level in d Glucose fed and restraint stress mouse models
    Neuropeptides, 2014
    Co-Authors: Soo-hyun Park, Jun-sub Jung
    Abstract:

    Abstract Alpha-melanocyte stimulating hormone (α-MSH) is known as a regulator of the blood Glucose homeostasis and food intake. In the present study, the possible roles of α-MSH located in the spinal cord in the regulation of the blood Glucose Level were investigated in d -Glucose-fed and immobilization stress (IMO) mouse models. We found in the present study that intrathecal (i.t.) injection with α-MSH alone did not affect the blood Glucose Level. However, i.t. administration with α-MSH reduced the blood Glucose Level in d -Glucose-fed model. The plasma insulin Level was increased in d -Glucose-fed model and was further increased by α-MSH, whereas α-MSH did not affect plasma corticosterone Level in d -Glucose-fed model. In addition, i.t. administration with glucagon alone enhanced blood Glucose Level and, i.t. injection with glucagon also increased the blood Glucose Level in d -Glucose-fed model. In contrasted to results observed in d -Glucose-fed model, i.t. treatment with α-MSH caused enhancement of the blood Glucose Level in IMO model. The plasma insulin Level was increased in IMO model. The increased plasma insulin Level by IMO was reduced by i.t. treatment with α-MSH, whereas i.t. pretreatment with α-MSH did not affect plasma corticosterone Level in IMO model. Taken together, although spinally located α-MSH itself does not alter the blood Glucose Level, our results suggest that the activation of α-MSH system located in the spinal cord play important modulatory roles for the reduction of the blood Glucose Level in d -Glucose fed model whereas α-MSH is responsible for the up-regulation of the blood Glucose Level in IMO model. The enhancement of insulin release may be responsible for modulatory action of α-MSH in down-regulation of the blood Glucose in d -Glucose fed model whereas reduction of insulin release may be responsible for modulatory action of α-MSH in up-regulation of the blood Glucose in IMO model.

  • the modulatory role of spinally located histamine receptors in the regulation of the blood Glucose Level in d Glucose fed mice
    The Korean Journal of Physiology and Pharmacology, 2014
    Co-Authors: Yun-beom Sim, Soo-hyun Park, Sung-su Kim, Jun-sub Jung, Chea-ha Kim, Su-jin Kim, Ohk-hyun Ryu, Moon-gi Choi, Sumin Lim, Hong-won Suh
    Abstract:

    The possible roles of spinal histamine receptors in the regulation of the blood Glucose Level were studied in ICR mice. Mice were intrathecally (i.t.) treated with histamine 1 (H1) receptor agonist (2-pyridylethylamine) or antagonist (cetirizine), histamine 2 (H2) receptor agonist (dimaprit) or antagonist (ranitidine), histamine 3 (H3) receptor agonist (α-methylhistamine) or antagonist (carcinine) and histamine 4 (H4) receptor agonist (VUF 8430) or antagonist (JNJ 7777120), and the blood Glucose Level was measured at 30, 60 and 120 min after i.t. administration. The i.t. injection with α-methylhistamine, but not carcinine slightly caused an elevation of the blood Glucose Level. In addition, histamine H1, H2, and H4 receptor agonists and antagonists did not affect the blood Glucose Level. In D-Glucose-fed model, i.t. pretreatment with cetirizine enhanced the blood Glucose Level, whereas 2-pyridylethylamine did not affect. The i.t. pretreatment with dimaprit, but not ranitidine, enhanced the blood Glucose Level in D-Glucose-fed model. In addition, α-methylhistamine, but not carcinine, slightly but significantly enhanced the blood Glucose Level D-Glucose-fed model. Finally, i.t. pretreatment with JNJ 7777120, but not VUF 8430, slightly but significantly increased the blood Glucose Level. Although histamine receptors themselves located at the spinal cord do not exert any effect on the regulation of the blood Glucose Level, our results suggest that the activation of spinal histamine H2 receptors and the blockade of spinal histamine H1 or H3 receptors may play modulatory roles for up-regulation and down-regulation, respectively, of the blood Glucose Level in D-Glucose fed model.

  • Effect of GABA Receptor Agonists or Antagonists Injected Spinally on the Blood Glucose Level in Mice
    Neurochemical research, 2013
    Co-Authors: Yun-beom Sim, Soo-hyun Park, Sung-su Kim, Jun-sub Jung, Yu-jung Kang, Chea-ha Kim, Su-jin Kim, Ohk-hyun Ryu, Moon-gi Choi, Hong-won Suh
    Abstract:

    The possible roles of gamma-amino butyric acid (GABA) receptors located in the spinal cord for the regulation of the blood Glucose Level were studied in ICR mice. We found in the present study that intrathecal (i.t.) injection with baclofen (a GABAB receptor agonist; 1-10 μg/5 μl) or bicuculline (a GABAA receptor antagonist; 1-10 μg/5 μl) caused an elevation of the blood Glucose Level in a dose-dependent manner. The hyperglycemic effect induced by baclofen was more pronounced than that induced by bicuculline. However, muscimol (a GABAA receptor agonist; 1-5 μg/5 μl) or phaclofen (a GABAB receptor antagonist; 5-10 μg/5 μl) administered i.t. did not affect the blood Glucose Level. Baclofen-induced elevation of the blood Glucose was dose-dependently attenuated by phaclofen. Furthermore, i.t. pretreatment with pertussis toxin (PTX; 0.05 or 0.1 μg/5 μl) for 6 days dose-dependently reduced the hyperglycemic effect induced by baclofen. Our results suggest that GABAB receptors located in the spinal cord play important roles for the elevation of the blood Glucose Level. Spinally located PTX-sensitive G-proteins appear to be involved in hyperglycemic effect induced by baclofen. Furthermore, inactivation of GABAA receptors located in the spinal cord appears to be responsible for tonic up-regulation of the blood Glucose Level.

Yun-beom Sim - One of the best experts on this subject based on the ideXlab platform.

  • the role of spinally located dopamine d 2 receptors in the regulation of the blood Glucose Level in mice
    Pharmacological Reports, 2020
    Co-Authors: Jaeseung Hong, Yun-beom Sim, Jinghui Feng, Jinkoo Lee, Heejung Lee, Jungseok Park, Hong-won Suh
    Abstract:

    The possible role of dopamine D2 receptors located in the spinal cord in the regulation of the blood Glucose Level have not been investigated before. In the present study, the effect of D2 receptor agonist and antagonist administered intrathecal (it) injection on the blood Glucose Level were examined in the Institute of Cancer Research (ICR) mice. We found that it injection with carmoxirole (D2 receptor agonist) caused an elevation of the blood Glucose Level in a dose-dependent manner. Carmoxirole-induced increase of the blood Glucose was significantly attenuated by L-741,626 (D2 receptor antagonist). Previously, we indicated that intrathecal (it) treatment with 0.1 μg/5 μl pertussis toxin (PTX, a Gi/Go inhibitor) produces a hypoglycemic effect in ICR in a long-term manner. In the present study, it pretreatment with PTX for 6 days almost abolished the hyperglycemic effect induced by carmoxirole. The plasma insulin Level was elevated by carmoxirole, and L-741,626 or PTX pretreatment reduced carmoxirole-induced increment of the insulin Level. In addition, the plasma corticosterone Level was increased by carmoxirole but it pretreatment with L-741,626 or PTX did not affect carmoxirole-induced increment of the corticosterone Level. Our results suggest that D2 receptors located in the spinal cord play an important role in the elevation of the blood Glucose Level. Spinally located inhibitory G-proteins appear to be involved in hyperglycemic effect induced by carmoxirole.

  • The modulatory roles of oxyntomodulin and glucagon-like peptide 1 administered spinally in the regulation of the blood Glucose Level
    Neurochemical Journal, 2017
    Co-Authors: Yun-beom Sim, Soo-hyun Park, Jae-ryeong Lee, Sung-su Kim, Jun-sub Jung, Naveen Sharma, Hong-won Suh
    Abstract:

    Oxyntomodulin is known as a regulator of the blood Glucose homeostasis and food intake. In the present study, the possible roles of oxyntomodulin receptor (GCGR/GLP-1) located in the spinal cord for the regulation of the blood Glucose Level was investigated in ICR mice. We found in the present study that intrathecal (i.t.) injection with oxyntomodulin (from 1 to 10 μg) or glucagon-like peptide-1 (GLP-1; from 1 to 10 μg) alone did not affect the blood Glucose Level. However, i.t. administration with oxyntomodulin, but not GLP-1, caused a reduction of the blood Glucose Level in D-Glucose-fed model. The oxyntomodulininduced reduction of the blood Glucose Level in D-Glucose-fed model is followed by reduction of insulin Level. Furthermore, the i.t. co-administration of oxyntomodulin with GLP-1 caused a profound up-regulation of the blood Glucose Level. The profound hyperglycemic effect shown by co-treatment with oxyntomodulin and GLP-1 caused a profound up-regulation of plasma insulin Level. Our results suggest that the activation of oxyntomodulin system present in the spinal cord may play significant modulatory roles in the reduction of the blood Glucose Level in D-Glucose-fed model. Furthermore, GLP-1 located in the spinal cord shows a synergistic interaction with oxyntomodulin for producing hyperglycemia.

  • effect of histamine receptors agonists or antagonists administered intracerebroventricularly and intrathecally on the blood Glucose Level in immobilization stress model
    Animal Cells and Systems, 2015
    Co-Authors: Naveen Sharma, Soo-hyun Park, Jae-ryeong Lee, Yun-beom Sim, Sung-su Kim, Jun-sub Jung, Hong-won Suh
    Abstract:

    Effects of histamine receptors agonists or antagonists administered centrally (supraspinally or spinally) on immobilization stress-induced blood Glucose Levels were studied in Institute for Cancer Research mice. Mice were pretreated intracerebroventricularly (i.c.v.) or intrathecally (i.t.) with histamine (H1, H2, H3 and H4) receptor agonists or antagonists 10 min prior to immobilization stress (IMO) stimulation for 30 min. We found that the increased blood Glucose Level induced by IMO was attenuated by the i.c.v. pretreatment with α-methylhistamine (H3 agonist). However, H3 antagonist, H1, H2 and H4 receptor agonists and antagonists did not affect the blood Glucose Level. Also, i.t. pretreatment with α-methylhistamine or VUF 8430 attenuated the increased blood Glucose Level induced by IMO. In addition, cetirizine and carcinine enhanced the increased blood Glucose Level induced by IMO. However, the blood Glucose Level was not affected by H1 and H2 receptor agonists, H2 and H4 receptor antagonists. Cortico...

  • the modulatory role of spinally located histamine receptors in the regulation of the blood Glucose Level in d Glucose fed mice
    The Korean Journal of Physiology and Pharmacology, 2014
    Co-Authors: Yun-beom Sim, Soo-hyun Park, Sung-su Kim, Jun-sub Jung, Chea-ha Kim, Su-jin Kim, Ohk-hyun Ryu, Moon-gi Choi, Sumin Lim, Hong-won Suh
    Abstract:

    The possible roles of spinal histamine receptors in the regulation of the blood Glucose Level were studied in ICR mice. Mice were intrathecally (i.t.) treated with histamine 1 (H1) receptor agonist (2-pyridylethylamine) or antagonist (cetirizine), histamine 2 (H2) receptor agonist (dimaprit) or antagonist (ranitidine), histamine 3 (H3) receptor agonist (α-methylhistamine) or antagonist (carcinine) and histamine 4 (H4) receptor agonist (VUF 8430) or antagonist (JNJ 7777120), and the blood Glucose Level was measured at 30, 60 and 120 min after i.t. administration. The i.t. injection with α-methylhistamine, but not carcinine slightly caused an elevation of the blood Glucose Level. In addition, histamine H1, H2, and H4 receptor agonists and antagonists did not affect the blood Glucose Level. In D-Glucose-fed model, i.t. pretreatment with cetirizine enhanced the blood Glucose Level, whereas 2-pyridylethylamine did not affect. The i.t. pretreatment with dimaprit, but not ranitidine, enhanced the blood Glucose Level in D-Glucose-fed model. In addition, α-methylhistamine, but not carcinine, slightly but significantly enhanced the blood Glucose Level D-Glucose-fed model. Finally, i.t. pretreatment with JNJ 7777120, but not VUF 8430, slightly but significantly increased the blood Glucose Level. Although histamine receptors themselves located at the spinal cord do not exert any effect on the regulation of the blood Glucose Level, our results suggest that the activation of spinal histamine H2 receptors and the blockade of spinal histamine H1 or H3 receptors may play modulatory roles for up-regulation and down-regulation, respectively, of the blood Glucose Level in D-Glucose fed model.

  • Effect of GABA Receptor Agonists or Antagonists Injected Spinally on the Blood Glucose Level in Mice
    Neurochemical research, 2013
    Co-Authors: Yun-beom Sim, Soo-hyun Park, Sung-su Kim, Jun-sub Jung, Yu-jung Kang, Chea-ha Kim, Su-jin Kim, Ohk-hyun Ryu, Moon-gi Choi, Hong-won Suh
    Abstract:

    The possible roles of gamma-amino butyric acid (GABA) receptors located in the spinal cord for the regulation of the blood Glucose Level were studied in ICR mice. We found in the present study that intrathecal (i.t.) injection with baclofen (a GABAB receptor agonist; 1-10 μg/5 μl) or bicuculline (a GABAA receptor antagonist; 1-10 μg/5 μl) caused an elevation of the blood Glucose Level in a dose-dependent manner. The hyperglycemic effect induced by baclofen was more pronounced than that induced by bicuculline. However, muscimol (a GABAA receptor agonist; 1-5 μg/5 μl) or phaclofen (a GABAB receptor antagonist; 5-10 μg/5 μl) administered i.t. did not affect the blood Glucose Level. Baclofen-induced elevation of the blood Glucose was dose-dependently attenuated by phaclofen. Furthermore, i.t. pretreatment with pertussis toxin (PTX; 0.05 or 0.1 μg/5 μl) for 6 days dose-dependently reduced the hyperglycemic effect induced by baclofen. Our results suggest that GABAB receptors located in the spinal cord play important roles for the elevation of the blood Glucose Level. Spinally located PTX-sensitive G-proteins appear to be involved in hyperglycemic effect induced by baclofen. Furthermore, inactivation of GABAA receptors located in the spinal cord appears to be responsible for tonic up-regulation of the blood Glucose Level.

Soo-hyun Park - One of the best experts on this subject based on the ideXlab platform.

  • The modulatory roles of oxyntomodulin and glucagon-like peptide 1 administered spinally in the regulation of the blood Glucose Level
    Neurochemical Journal, 2017
    Co-Authors: Yun-beom Sim, Soo-hyun Park, Jae-ryeong Lee, Sung-su Kim, Jun-sub Jung, Naveen Sharma, Hong-won Suh
    Abstract:

    Oxyntomodulin is known as a regulator of the blood Glucose homeostasis and food intake. In the present study, the possible roles of oxyntomodulin receptor (GCGR/GLP-1) located in the spinal cord for the regulation of the blood Glucose Level was investigated in ICR mice. We found in the present study that intrathecal (i.t.) injection with oxyntomodulin (from 1 to 10 μg) or glucagon-like peptide-1 (GLP-1; from 1 to 10 μg) alone did not affect the blood Glucose Level. However, i.t. administration with oxyntomodulin, but not GLP-1, caused a reduction of the blood Glucose Level in D-Glucose-fed model. The oxyntomodulininduced reduction of the blood Glucose Level in D-Glucose-fed model is followed by reduction of insulin Level. Furthermore, the i.t. co-administration of oxyntomodulin with GLP-1 caused a profound up-regulation of the blood Glucose Level. The profound hyperglycemic effect shown by co-treatment with oxyntomodulin and GLP-1 caused a profound up-regulation of plasma insulin Level. Our results suggest that the activation of oxyntomodulin system present in the spinal cord may play significant modulatory roles in the reduction of the blood Glucose Level in D-Glucose-fed model. Furthermore, GLP-1 located in the spinal cord shows a synergistic interaction with oxyntomodulin for producing hyperglycemia.

  • effect of histamine receptors agonists or antagonists administered intracerebroventricularly and intrathecally on the blood Glucose Level in immobilization stress model
    Animal Cells and Systems, 2015
    Co-Authors: Naveen Sharma, Soo-hyun Park, Jae-ryeong Lee, Yun-beom Sim, Sung-su Kim, Jun-sub Jung, Hong-won Suh
    Abstract:

    Effects of histamine receptors agonists or antagonists administered centrally (supraspinally or spinally) on immobilization stress-induced blood Glucose Levels were studied in Institute for Cancer Research mice. Mice were pretreated intracerebroventricularly (i.c.v.) or intrathecally (i.t.) with histamine (H1, H2, H3 and H4) receptor agonists or antagonists 10 min prior to immobilization stress (IMO) stimulation for 30 min. We found that the increased blood Glucose Level induced by IMO was attenuated by the i.c.v. pretreatment with α-methylhistamine (H3 agonist). However, H3 antagonist, H1, H2 and H4 receptor agonists and antagonists did not affect the blood Glucose Level. Also, i.t. pretreatment with α-methylhistamine or VUF 8430 attenuated the increased blood Glucose Level induced by IMO. In addition, cetirizine and carcinine enhanced the increased blood Glucose Level induced by IMO. However, the blood Glucose Level was not affected by H1 and H2 receptor agonists, H2 and H4 receptor antagonists. Cortico...

  • the modulatory role of alpha melanocyte stimulating hormone administered spinally in the regulation of blood Glucose Level in d Glucose fed and restraint stress mouse models
    Neuropeptides, 2014
    Co-Authors: Soo-hyun Park, Jun-sub Jung
    Abstract:

    Abstract Alpha-melanocyte stimulating hormone (α-MSH) is known as a regulator of the blood Glucose homeostasis and food intake. In the present study, the possible roles of α-MSH located in the spinal cord in the regulation of the blood Glucose Level were investigated in d -Glucose-fed and immobilization stress (IMO) mouse models. We found in the present study that intrathecal (i.t.) injection with α-MSH alone did not affect the blood Glucose Level. However, i.t. administration with α-MSH reduced the blood Glucose Level in d -Glucose-fed model. The plasma insulin Level was increased in d -Glucose-fed model and was further increased by α-MSH, whereas α-MSH did not affect plasma corticosterone Level in d -Glucose-fed model. In addition, i.t. administration with glucagon alone enhanced blood Glucose Level and, i.t. injection with glucagon also increased the blood Glucose Level in d -Glucose-fed model. In contrasted to results observed in d -Glucose-fed model, i.t. treatment with α-MSH caused enhancement of the blood Glucose Level in IMO model. The plasma insulin Level was increased in IMO model. The increased plasma insulin Level by IMO was reduced by i.t. treatment with α-MSH, whereas i.t. pretreatment with α-MSH did not affect plasma corticosterone Level in IMO model. Taken together, although spinally located α-MSH itself does not alter the blood Glucose Level, our results suggest that the activation of α-MSH system located in the spinal cord play important modulatory roles for the reduction of the blood Glucose Level in d -Glucose fed model whereas α-MSH is responsible for the up-regulation of the blood Glucose Level in IMO model. The enhancement of insulin release may be responsible for modulatory action of α-MSH in down-regulation of the blood Glucose in d -Glucose fed model whereas reduction of insulin release may be responsible for modulatory action of α-MSH in up-regulation of the blood Glucose in IMO model.

  • the modulatory role of spinally located histamine receptors in the regulation of the blood Glucose Level in d Glucose fed mice
    The Korean Journal of Physiology and Pharmacology, 2014
    Co-Authors: Yun-beom Sim, Soo-hyun Park, Sung-su Kim, Jun-sub Jung, Chea-ha Kim, Su-jin Kim, Ohk-hyun Ryu, Moon-gi Choi, Sumin Lim, Hong-won Suh
    Abstract:

    The possible roles of spinal histamine receptors in the regulation of the blood Glucose Level were studied in ICR mice. Mice were intrathecally (i.t.) treated with histamine 1 (H1) receptor agonist (2-pyridylethylamine) or antagonist (cetirizine), histamine 2 (H2) receptor agonist (dimaprit) or antagonist (ranitidine), histamine 3 (H3) receptor agonist (α-methylhistamine) or antagonist (carcinine) and histamine 4 (H4) receptor agonist (VUF 8430) or antagonist (JNJ 7777120), and the blood Glucose Level was measured at 30, 60 and 120 min after i.t. administration. The i.t. injection with α-methylhistamine, but not carcinine slightly caused an elevation of the blood Glucose Level. In addition, histamine H1, H2, and H4 receptor agonists and antagonists did not affect the blood Glucose Level. In D-Glucose-fed model, i.t. pretreatment with cetirizine enhanced the blood Glucose Level, whereas 2-pyridylethylamine did not affect. The i.t. pretreatment with dimaprit, but not ranitidine, enhanced the blood Glucose Level in D-Glucose-fed model. In addition, α-methylhistamine, but not carcinine, slightly but significantly enhanced the blood Glucose Level D-Glucose-fed model. Finally, i.t. pretreatment with JNJ 7777120, but not VUF 8430, slightly but significantly increased the blood Glucose Level. Although histamine receptors themselves located at the spinal cord do not exert any effect on the regulation of the blood Glucose Level, our results suggest that the activation of spinal histamine H2 receptors and the blockade of spinal histamine H1 or H3 receptors may play modulatory roles for up-regulation and down-regulation, respectively, of the blood Glucose Level in D-Glucose fed model.

  • Effect of GABA Receptor Agonists or Antagonists Injected Spinally on the Blood Glucose Level in Mice
    Neurochemical research, 2013
    Co-Authors: Yun-beom Sim, Soo-hyun Park, Sung-su Kim, Jun-sub Jung, Yu-jung Kang, Chea-ha Kim, Su-jin Kim, Ohk-hyun Ryu, Moon-gi Choi, Hong-won Suh
    Abstract:

    The possible roles of gamma-amino butyric acid (GABA) receptors located in the spinal cord for the regulation of the blood Glucose Level were studied in ICR mice. We found in the present study that intrathecal (i.t.) injection with baclofen (a GABAB receptor agonist; 1-10 μg/5 μl) or bicuculline (a GABAA receptor antagonist; 1-10 μg/5 μl) caused an elevation of the blood Glucose Level in a dose-dependent manner. The hyperglycemic effect induced by baclofen was more pronounced than that induced by bicuculline. However, muscimol (a GABAA receptor agonist; 1-5 μg/5 μl) or phaclofen (a GABAB receptor antagonist; 5-10 μg/5 μl) administered i.t. did not affect the blood Glucose Level. Baclofen-induced elevation of the blood Glucose was dose-dependently attenuated by phaclofen. Furthermore, i.t. pretreatment with pertussis toxin (PTX; 0.05 or 0.1 μg/5 μl) for 6 days dose-dependently reduced the hyperglycemic effect induced by baclofen. Our results suggest that GABAB receptors located in the spinal cord play important roles for the elevation of the blood Glucose Level. Spinally located PTX-sensitive G-proteins appear to be involved in hyperglycemic effect induced by baclofen. Furthermore, inactivation of GABAA receptors located in the spinal cord appears to be responsible for tonic up-regulation of the blood Glucose Level.

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  • effect of luffa aegyptiaca seeds and carissa edulis leaves extracts on blood Glucose Level of normal and streptozotocin diabetic rats
    Journal of Ethnopharmacology, 1996
    Co-Authors: Fathy K Elfiky, Mohamed A Aboukaram, Elham A Afify
    Abstract:

    The present study investigates the effect of oral administration of the ethanolic extracts of Luffa aegyptiaca (seeds) and Carissa edulis (leaves) on blood Glucose Levels both in normal and streptozotocin (STZ) diabetic rats. Treatment with both extracts significantly reduced the blood Glucose Level in STZ diabetic rats during the first three hours of treatment. L. aegyptiaca extract decreased blood Glucose Level with a potency similar to that of the biguanide, metformin. The total glycaemic areas were 589.61 ± 45.62 mg/dl/3 h and 660.38 ± 64.44 mg/dl/3 h for L. aegyptiaca and metformin, respectively, vs. 816.73 ± 43.21 mg/dl/3 h for the control (P < 0.05). On the other hand, in normal rats, both treatments produced insignificant changes in blood Glucose Levels compared to glibenclamide treatment.