The Experts below are selected from a list of 276 Experts worldwide ranked by ideXlab platform
Katarzyna Winnicka - One of the best experts on this subject based on the ideXlab platform.
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Novel Spray Dried Glycerol 2-Phosphate Cross-Linked Chitosan Microparticulate Vaginal Delivery System—Development, Characterization and Cytotoxicity Studies
Marine drugs, 2016Co-Authors: Emilia Szymańska, Marta Szekalska, Robert Czarnomysy, Zoran Lavrič, Stane Srčič, Wojciech Miltyk, Katarzyna WinnickaAbstract:Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using Glycerol 2-Phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of Glycerol 2-Phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the Glycerol 2-Phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.
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novel spray dried Glycerol 2 Phosphate cross linked chitosan microparticulate vaginal delivery system development characterization and cytotoxicity studies
Marine Drugs, 2016Co-Authors: Emilia Szymańska, Marta Szekalska, Robert Czarnomysy, Zoran Lavrič, Stane Srčič, Wojciech Miltyk, Katarzyna WinnickaAbstract:Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using Glycerol 2-Phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of Glycerol 2-Phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the Glycerol 2-Phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.
Emilia Szymańska - One of the best experts on this subject based on the ideXlab platform.
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Novel Spray Dried Glycerol 2-Phosphate Cross-Linked Chitosan Microparticulate Vaginal Delivery System—Development, Characterization and Cytotoxicity Studies
Marine drugs, 2016Co-Authors: Emilia Szymańska, Marta Szekalska, Robert Czarnomysy, Zoran Lavrič, Stane Srčič, Wojciech Miltyk, Katarzyna WinnickaAbstract:Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using Glycerol 2-Phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of Glycerol 2-Phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the Glycerol 2-Phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.
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novel spray dried Glycerol 2 Phosphate cross linked chitosan microparticulate vaginal delivery system development characterization and cytotoxicity studies
Marine Drugs, 2016Co-Authors: Emilia Szymańska, Marta Szekalska, Robert Czarnomysy, Zoran Lavrič, Stane Srčič, Wojciech Miltyk, Katarzyna WinnickaAbstract:Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using Glycerol 2-Phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of Glycerol 2-Phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the Glycerol 2-Phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.
Lynne E. Macaskie - One of the best experts on this subject based on the ideXlab platform.
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Eu3+ Sequestration by Biogenic Nano-Hydroxyapatite Synthesized at Neutral and Alkaline pH
Geomicrobiology Journal, 2017Co-Authors: Rajkumar Gangappa, Adam Farrier, Lynne E. MacaskieAbstract:ABSTRACTBiogenic hydroxyapatite (bio-HA) has the potential for radionuclide capture and remediation of metal-contaminated environments. Biosynthesis of bio-HA was achieved via the phosphatase activity of a Serratia sp. supplemented with various concentrations of CaCl2 and Glycerol 2-Phosphate (G2P) provided at pH 7.0 or 8.6. Presence of hydroxyapatite (HA) was confirmed in the samples by X-ray powder diffraction analysis. When provided with limiting (1 mM) G2P and excess (5 mM) Ca2+ at pH 8.6, monohydrocalcite was found. This, and bio-HA with less (1 mM) Ca2+ accumulated Eu(III) to ∼31% and 20% of the biomineral mass, respectively, as compared to 50% of the mineral mass accumulated by commercial HA. Optimally, with bio-HA made at initial pH 7.0 from 2 mM Ca2+ and 5 mM G2P, Eu(III) accumulated to ∼74% of the weight of bio-HA, which was equal to the mass of the HA mineral component of the biomaterial. The implications with respect to potential bio-HA-barrier development in situ or as a remediation strategy ...
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Hydroxyapatite Biosynthesis by a Serratia sp. and Application of Nanoscale Bio-HA in the Recovery of Strontium and Europium
Geomicrobiology Journal, 2015Co-Authors: Rajkumar Gangappa, Ping Yong, Sarah Singh, I.p. Mikheenko, Angela J. Murray, Lynne E. MacaskieAbstract:ABSTRACTA Serratia sp. expresses a high level of acid phosphatase when grown continuously under carbon limitation. In the presence of CaCl2, biosynthesis of nanocrystalline hydroxyapatite (bio-HA) was achieved by utilizing Phosphate released via enzymatic cleavage of an applied substrate (Glycerol 2-Phosphate: G2P). Hydroxyapatite crystals were identified by energy dispersive X-ray emission (EDX) and selected area diffraction (SAD). X-ray powder diffraction (XRD) analysis gave a mean crystallite size of ∼21–32 nm, with the smallest crystals (21–24 nm) obtained using 1 mM Ca2+ and 1 mM G2P. The uptake of Eu3+ and Sr2+ by bio-HA made by continuously pregrown cells (0.42 mg/mg and 0.043 mg/mg respectively) was ∼20% greater for Sr2+ than was previously reported for bio-HA material of size ∼40 nm made by batch-pregrown cells, while the corresponding uptake of Eu3+ was increased by > 1.8-fold. This was attributed to the localization of Eu (III) at grain boundaries by reference to previous work and highlights th...
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Biorecovery of Rare Earth Elements: Potential Application for Mine Water Remediation
Advanced Materials Research, 2015Co-Authors: Angela J. Murray, Sarah Singh, Mark Robert Tolley, Lynne E. MacaskieAbstract:Rare earth elements (REEs) are highly valuable due to the complex nature of their extraction from primary and secondary sources. A key feature is that REEs often co-occur with uranium and thorium which, being radioactive, increase the hazard and complexity of REE recovery. A bioprocess which utilizes enzymatically-generated inorganic Phosphate to precipitate REEs from solution as their Phosphate biominerals is highly effective in the recovery of REEs, effecting rapid recovery onto immobilized bacterial biofilm at high flow-through rates. This also bioprecipitates U and Th. The metal recovery process requires addition of an organic Phosphate substrate, e.g. Glycerol 2-Phosphate (G2P), the cleavage of which provides the inorganic Phosphate source for REE biomineralization. G2P is expensive, precluding its large scale use, but early work using uranium showed that tributyl Phosphate (TBP) can be used as an alternative Phosphate donor molecule. The potential for substitution of G2P by TBP for biorecovery of neodymium is described and a new approach is proposed for enhancing the metal selectivity for REEs against uranium.
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A Study of Biofilm and Non-Line-of-Sight Bio-Hydroxyapatite Coatings Using a Serratia sp.
Advanced Materials Research, 2009Co-Authors: Ping Yong, Marion Paterson-beedle, W. Liu, Zhibing Zhang, D.a. Beauregard, Michael L. Johns, Lynne E. MacaskieAbstract:This study describes biofilm formation as a non line-of-sight coating method on support materials such as polyurethane foam, porous glass, polypropylene (PP) and titanium alloy, using a Serratia sp., which can manufacture extracellular nanoscale scaffolded hydroxyapatite (HA) crystals via enzymatic cleavage of Glycerol 2-Phosphate (G2P) in the presence of CaCl2. Various microscopies and non-invasive magnetic resonance imaging were used to visualize the biofilm coating on the support surface. A novel micromanipulation technique was used to estimate the adhesive strength of native and HA-mineralized biofilms. The biofilm with HA was up to forty times stronger than that without HA. A coating of nano-HA (> 80 m) onto a biofilm-Ti disc was achieved.
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Phosphotransferase activity of acid phosphatases of a Citrobacter sp.
FEMS Microbiology Letters, 2006Co-Authors: B.c. Jeong, H.-w Kim, Lynne E. MacaskieAbstract:The acid phosphatase of an atypical Citrobacter sp. was purified in two isoforms, designated CPI and CPII, which had different Km values for Glycerol 1-Phosphate and Glycerol 2-Phosphate The enzyme was not inhibited by the end-product Glycerol. Enzyme activity was increased in the presence of Phosphate acceptor molecules having free hydroxyl groups (Glycerol, methanol, ethanol). 31P-nuclear magnetic resonance spectroscopy indicated transfer of the liberated Phosphate onto the alcohol, with the de novo production of (e.g.) Glycerol 1-Phosphate by enzyme supplemented with phosphomonoester substrate and Glycerol.
Marta Szekalska - One of the best experts on this subject based on the ideXlab platform.
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Novel Spray Dried Glycerol 2-Phosphate Cross-Linked Chitosan Microparticulate Vaginal Delivery System—Development, Characterization and Cytotoxicity Studies
Marine drugs, 2016Co-Authors: Emilia Szymańska, Marta Szekalska, Robert Czarnomysy, Zoran Lavrič, Stane Srčič, Wojciech Miltyk, Katarzyna WinnickaAbstract:Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using Glycerol 2-Phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of Glycerol 2-Phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the Glycerol 2-Phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.
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novel spray dried Glycerol 2 Phosphate cross linked chitosan microparticulate vaginal delivery system development characterization and cytotoxicity studies
Marine Drugs, 2016Co-Authors: Emilia Szymańska, Marta Szekalska, Robert Czarnomysy, Zoran Lavrič, Stane Srčič, Wojciech Miltyk, Katarzyna WinnickaAbstract:Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using Glycerol 2-Phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of Glycerol 2-Phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the Glycerol 2-Phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.
Robert Czarnomysy - One of the best experts on this subject based on the ideXlab platform.
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Novel Spray Dried Glycerol 2-Phosphate Cross-Linked Chitosan Microparticulate Vaginal Delivery System—Development, Characterization and Cytotoxicity Studies
Marine drugs, 2016Co-Authors: Emilia Szymańska, Marta Szekalska, Robert Czarnomysy, Zoran Lavrič, Stane Srčič, Wojciech Miltyk, Katarzyna WinnickaAbstract:Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using Glycerol 2-Phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of Glycerol 2-Phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the Glycerol 2-Phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.
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novel spray dried Glycerol 2 Phosphate cross linked chitosan microparticulate vaginal delivery system development characterization and cytotoxicity studies
Marine Drugs, 2016Co-Authors: Emilia Szymańska, Marta Szekalska, Robert Czarnomysy, Zoran Lavrič, Stane Srčič, Wojciech Miltyk, Katarzyna WinnickaAbstract:Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using Glycerol 2-Phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of Glycerol 2-Phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the Glycerol 2-Phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.