The Experts below are selected from a list of 2325 Experts worldwide ranked by ideXlab platform

Keiji Maruoka - One of the best experts on this subject based on the ideXlab platform.

Maciej Serda - One of the best experts on this subject based on the ideXlab platform.

  • a 60 fullerene nanoconjugate with gemcitabine synthesis biophysical properties and biological evaluation for treating pancreatic cancer
    Cancer Nanotechnology, 2020
    Co-Authors: Pawel Nalepa, Robert Gawecki, Grzegorz Szewczyk, Katarzyna Balin, Mateusz Dulski, Mieczyslaw Sajewicz, Anna Mrozekwilczkiewicz, Robert Musiol, Jaroslaw Polanski, Maciej Serda
    Abstract:

    The first-line chemotherapy drug that is used to treat pancreatic ductal adenocarcinoma is gemcitabine. Unfortunately, its effectiveness is hampered by its chemo-resistance, low vascularization and drug biodistribution limitations in the tumor microenvironment. Novel nanotherapeutics must be developed in order to improve the prognosis for patients with pancreatic cancer. We developed a synthetic methodology for obtaining a water-soluble nanoconjugate of a [60]fullerene-Glycine Derivative with the FDA-approved drug gemcitabine (nanoC60GEM). The proposed synthetic protocol enables a highly water-soluble [60]fullerene-Glycine Derivative (6) to be obtained, which was next successfully conjugated with gemcitabine using the EDCI/NHS carbodiimide protocol. The desired nanoconjugate was characterized using mass spectrometry and DLS, IR and XPS techniques. The photogeneration of singlet oxygen and the superoxide anion radical were studied by measuring 1O2 near-infrared luminescence at 1270 nm, followed by spin trapping of the DMPO adducts by EPR spectroscopy. The biological assays that were performed indicate that there is an inhibition of the cell cycle in the S phase and the induction of apoptosis by nanoC60GEM. In this paper, we present a robust approach for synthesizing a highly water-soluble [60]fullerene nanoconjugate with gemcitabine. The performed biological assays on pancreatic cancer cell lines demonstrated cytotoxic effects of nanoC60GEM, which were enhanced by the generation of reactive oxygen species after blue LED irradiation of synthesized fullerene nanomaterial.

  • A [60]fullerene nanoconjugate with gemcitabine: synthesis, biophysical properties and biological evaluation for treating pancreatic cancer
    Cancer Nanotechnology, 2020
    Co-Authors: Paweł Nalepa, Robert Gawecki, Grzegorz Szewczyk, Katarzyna Balin, Mateusz Dulski, Mieczyslaw Sajewicz, Jaroslaw Polanski, Anna Mrozek-wilczkiewicz, Robert Musioł, Maciej Serda
    Abstract:

    Background The first-line chemotherapy drug that is used to treat pancreatic ductal adenocarcinoma is gemcitabine. Unfortunately, its effectiveness is hampered by its chemo-resistance, low vascularization and drug biodistribution limitations in the tumor microenvironment. Novel nanotherapeutics must be developed in order to improve the prognosis for patients with pancreatic cancer. Results We developed a synthetic methodology for obtaining a water-soluble nanoconjugate of a [60]fullerene-Glycine Derivative with the FDA-approved drug gemcitabine ( nanoC _ 60 GEM) . The proposed synthetic protocol enables a highly water-soluble [60]fullerene-Glycine Derivative (6) to be obtained, which was next successfully conjugated with gemcitabine using the EDCI/NHS carbodiimide protocol. The desired nanoconjugate was characterized using mass spectrometry and DLS, IR and XPS techniques. The photogeneration of singlet oxygen and the superoxide anion radical were studied by measuring ^1O_2 near-infrared luminescence at 1270 nm, followed by spin trapping of the DMPO adducts by EPR spectroscopy. The biological assays that were performed indicate that there is an inhibition of the cell cycle in the S phase and the induction of apoptosis by nanoC_60GEM. Conclusion In this paper, we present a robust approach for synthesizing a highly water-soluble [60]fullerene nanoconjugate with gemcitabine. The performed biological assays on pancreatic cancer cell lines demonstrated cytotoxic effects of nanoC_60GEM, which were enhanced by the generation of reactive oxygen species after blue LED irradiation of synthesized fullerene nanomaterial.

Seiji Shirakawa - One of the best experts on this subject based on the ideXlab platform.

Hweihsien Chen - One of the best experts on this subject based on the ideXlab platform.

  • effects of sarcosine and n n dimethylGlycine on nmda receptor mediated excitatory field potentials
    Journal of Biomedical Science, 2017
    Co-Authors: Yishu Tu, Yufeng J Tseng, Minghuan Chan, Hweihsien Chen
    Abstract:

    Sarcosine, a Glycine transporter type 1 inhibitor and an N-methyl-D-aspartate (NMDA) receptor co-agonist at the Glycine binding site, potentiates NMDA receptor function. Structurally similar to sarcosine, N,N-dimethylGlycine (DMG) is also N-methyl Glycine-Derivative amino acid and commonly used as a dietary supplement. The present study compared the effects of sarcosine and DMG on NMDA receptor-mediated excitatory field potentials (EFPs) in mouse medial prefrontal cortex brain slices using a multi-electrode array system. Glycine, sarcosine and DMG alone did not alter the NMDA receptor-mediated EFPs, but in combination with glutamate, Glycine and its N-methyl Derivatives significantly increased the frequency and amplitude of EFPs. The enhancing effects of Glycine analogs in combination with glutamate on EFPs were remarkably reduced by the Glycine binding site antagonist 7-chlorokynurenate (7-CK). However, DMG, but not sarcosine, reduced the frequency and amplitude of EFPs elicited by co-application of glutamate plus Glycine. D-cycloserine, a partial agonist at the Glycine binding site on NMDA receptors, affected EFPs in a similar manner to DMG. Furthermore, DMG, but not sarcosine, reduced the frequencies and amplitudes of EFPs elicited by glutamate plus D-serine, another endogenous ligand for Glycine binding site. These findings suggest that sarcosine acts as a full agonist, yet DMG is a partial agonist at Glycine binding site of NMDA receptors. The molecular docking analysis indicated that the interactions of Glycine, sarcosine, and DMG to NMDA receptors are highly similar, supporting that the Glycine binding site of NMDA receptors is a critical target site for sarcosine and DMG.

Jaroslaw Polanski - One of the best experts on this subject based on the ideXlab platform.

  • a 60 fullerene nanoconjugate with gemcitabine synthesis biophysical properties and biological evaluation for treating pancreatic cancer
    Cancer Nanotechnology, 2020
    Co-Authors: Pawel Nalepa, Robert Gawecki, Grzegorz Szewczyk, Katarzyna Balin, Mateusz Dulski, Mieczyslaw Sajewicz, Anna Mrozekwilczkiewicz, Robert Musiol, Jaroslaw Polanski, Maciej Serda
    Abstract:

    The first-line chemotherapy drug that is used to treat pancreatic ductal adenocarcinoma is gemcitabine. Unfortunately, its effectiveness is hampered by its chemo-resistance, low vascularization and drug biodistribution limitations in the tumor microenvironment. Novel nanotherapeutics must be developed in order to improve the prognosis for patients with pancreatic cancer. We developed a synthetic methodology for obtaining a water-soluble nanoconjugate of a [60]fullerene-Glycine Derivative with the FDA-approved drug gemcitabine (nanoC60GEM). The proposed synthetic protocol enables a highly water-soluble [60]fullerene-Glycine Derivative (6) to be obtained, which was next successfully conjugated with gemcitabine using the EDCI/NHS carbodiimide protocol. The desired nanoconjugate was characterized using mass spectrometry and DLS, IR and XPS techniques. The photogeneration of singlet oxygen and the superoxide anion radical were studied by measuring 1O2 near-infrared luminescence at 1270 nm, followed by spin trapping of the DMPO adducts by EPR spectroscopy. The biological assays that were performed indicate that there is an inhibition of the cell cycle in the S phase and the induction of apoptosis by nanoC60GEM. In this paper, we present a robust approach for synthesizing a highly water-soluble [60]fullerene nanoconjugate with gemcitabine. The performed biological assays on pancreatic cancer cell lines demonstrated cytotoxic effects of nanoC60GEM, which were enhanced by the generation of reactive oxygen species after blue LED irradiation of synthesized fullerene nanomaterial.

  • A [60]fullerene nanoconjugate with gemcitabine: synthesis, biophysical properties and biological evaluation for treating pancreatic cancer
    Cancer Nanotechnology, 2020
    Co-Authors: Paweł Nalepa, Robert Gawecki, Grzegorz Szewczyk, Katarzyna Balin, Mateusz Dulski, Mieczyslaw Sajewicz, Jaroslaw Polanski, Anna Mrozek-wilczkiewicz, Robert Musioł, Maciej Serda
    Abstract:

    Background The first-line chemotherapy drug that is used to treat pancreatic ductal adenocarcinoma is gemcitabine. Unfortunately, its effectiveness is hampered by its chemo-resistance, low vascularization and drug biodistribution limitations in the tumor microenvironment. Novel nanotherapeutics must be developed in order to improve the prognosis for patients with pancreatic cancer. Results We developed a synthetic methodology for obtaining a water-soluble nanoconjugate of a [60]fullerene-Glycine Derivative with the FDA-approved drug gemcitabine ( nanoC _ 60 GEM) . The proposed synthetic protocol enables a highly water-soluble [60]fullerene-Glycine Derivative (6) to be obtained, which was next successfully conjugated with gemcitabine using the EDCI/NHS carbodiimide protocol. The desired nanoconjugate was characterized using mass spectrometry and DLS, IR and XPS techniques. The photogeneration of singlet oxygen and the superoxide anion radical were studied by measuring ^1O_2 near-infrared luminescence at 1270 nm, followed by spin trapping of the DMPO adducts by EPR spectroscopy. The biological assays that were performed indicate that there is an inhibition of the cell cycle in the S phase and the induction of apoptosis by nanoC_60GEM. Conclusion In this paper, we present a robust approach for synthesizing a highly water-soluble [60]fullerene nanoconjugate with gemcitabine. The performed biological assays on pancreatic cancer cell lines demonstrated cytotoxic effects of nanoC_60GEM, which were enhanced by the generation of reactive oxygen species after blue LED irradiation of synthesized fullerene nanomaterial.