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Anna Wanahita - One of the best experts on this subject based on the ideXlab platform.
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diagnostic value of microscopic examination of Gram stained sputum and sputum cultures in patients with bacteremic pneumococcal pneumonia
Clinical Infectious Diseases, 2004Co-Authors: Daniel M Musher, Roberto Montoya, Anna WanahitaAbstract:Clinicians continue to question the usefulness of microscopic examination of Gram-stained sputum specimens ("Gram Staining") and sputum culture for diagnosis of pneumonia. We analyzed the sensitivity of these techniques in 105 patients with pneumococcal pneumonia proven by blood culture. Gram Staining revealed Gram-positive cocci in pairs and chains, and culture yielded pneumococci in only 31% and 44% of all cases, respectively. However, sputum specimens were never submitted for examination in 31 cases; in 16 others, the specimen was inadequate and a culture was not done. Excluding these cases, the sensitivities of Gram Staining and culture were 57% and 79%, respectively. If patients receiving antibiotics for >24 h had been excluded, Gram Staining would have suggested pneumococci in 63%, and culture results would have been positive in 86%. Sensitivity increased in inverse proportion to the duration of antibiotic therapy (P 80% of cases of pneumococcal pneumonia.
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diagnostic value of microscopic examination of Gram stained sputum and sputum cultures in patients with bacteremic pneumococcal pneumonia
Clinical Infectious Diseases, 2004Co-Authors: Daniel M Musher, Roberto Montoya, Anna WanahitaAbstract:Clinicians continue to question the usefulness of microscopic examination of Gram-stained sputum specimens (“Gram Staining”) and sputum culture for diagnosis of pneumonia. We analyzed the sensitivity of these techniques in 105 patients with pneumococcal pneumonia proven by blood culture. Gram Staining revealed Gram-positive cocci in pairs and chains, and culture yielded pneumococci in only 31% and 44% of all cases, respectively. However, sputum specimens were never submitted for examination in 31 cases; in 16 others, the specimen was inadequate and a culture was not done. Excluding these cases, the sensitivities of Gram Staining and culture were 57% and 79%, respectively. If patients receiving antibiotics for 124 h had been excluded, Gram Staining would have suggested pneumococci in 63%, and culture results would have been positive in 86%. Sensitivity increased in inverse proportion to the duration of antibiotic therapy ( ). Microscopic P ! .05 examination of sputum samples before antibiotics were administered and performance of culture within 24 h of receipt of such treatment yielded the correct diagnosis in 180% of cases of pneumococcal pneumonia.
Daniel M Musher - One of the best experts on this subject based on the ideXlab platform.
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diagnostic value of microscopic examination of Gram stained sputum and sputum cultures in patients with bacteremic pneumococcal pneumonia
Clinical Infectious Diseases, 2004Co-Authors: Daniel M Musher, Roberto Montoya, Anna WanahitaAbstract:Clinicians continue to question the usefulness of microscopic examination of Gram-stained sputum specimens ("Gram Staining") and sputum culture for diagnosis of pneumonia. We analyzed the sensitivity of these techniques in 105 patients with pneumococcal pneumonia proven by blood culture. Gram Staining revealed Gram-positive cocci in pairs and chains, and culture yielded pneumococci in only 31% and 44% of all cases, respectively. However, sputum specimens were never submitted for examination in 31 cases; in 16 others, the specimen was inadequate and a culture was not done. Excluding these cases, the sensitivities of Gram Staining and culture were 57% and 79%, respectively. If patients receiving antibiotics for >24 h had been excluded, Gram Staining would have suggested pneumococci in 63%, and culture results would have been positive in 86%. Sensitivity increased in inverse proportion to the duration of antibiotic therapy (P 80% of cases of pneumococcal pneumonia.
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diagnostic value of microscopic examination of Gram stained sputum and sputum cultures in patients with bacteremic pneumococcal pneumonia
Clinical Infectious Diseases, 2004Co-Authors: Daniel M Musher, Roberto Montoya, Anna WanahitaAbstract:Clinicians continue to question the usefulness of microscopic examination of Gram-stained sputum specimens (“Gram Staining”) and sputum culture for diagnosis of pneumonia. We analyzed the sensitivity of these techniques in 105 patients with pneumococcal pneumonia proven by blood culture. Gram Staining revealed Gram-positive cocci in pairs and chains, and culture yielded pneumococci in only 31% and 44% of all cases, respectively. However, sputum specimens were never submitted for examination in 31 cases; in 16 others, the specimen was inadequate and a culture was not done. Excluding these cases, the sensitivities of Gram Staining and culture were 57% and 79%, respectively. If patients receiving antibiotics for 124 h had been excluded, Gram Staining would have suggested pneumococci in 63%, and culture results would have been positive in 86%. Sensitivity increased in inverse proportion to the duration of antibiotic therapy ( ). Microscopic P ! .05 examination of sputum samples before antibiotics were administered and performance of culture within 24 h of receipt of such treatment yielded the correct diagnosis in 180% of cases of pneumococcal pneumonia.
Marc Lecuit - One of the best experts on this subject based on the ideXlab platform.
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disseminated spiroplasma apis infection in patient with agammaglobulinemia france
Emerging Infectious Diseases, 2018Co-Authors: Nicolas Etienne, Laurent Bret, Cecile Le Brun, Herve Lecuyer, Josquin Moraly, Agnes Ferroni, Marc Lecuit, Fanny Lanternier, Olivier HermineAbstract:: We report a disseminated infection caused by Spiroplasma apis, a honeybee pathogen, in a patient in France who had X-linked agammaglobulinemia. Identification was challenging because initial bacterial cultures and direct examination by Gram Staining were negative. Unexplained sepsis in patients with agammaglobulinemia warrants specific investigation to identify fastidious bacteria such as Spiroplasma spp.
Tsuneaki Kenzaka - One of the best experts on this subject based on the ideXlab platform.
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bacteriological testing and recurrence prevention efforts in the diagnosis and treatment of nursing and healthcare associated pneumonia and aspiration pneumonia a questionnaire survey of hospitals across japan
Respiratory investigation, 2018Co-Authors: Tsuneaki Kenzaka, Ayako Kumabe, Koki Kosami, Yasufumi Matsuoka, Kensuke Minami, Daisuke Ninomiya, Ayako Noda, Shinsuke YahataAbstract:Abstract Background Clinical practice guidelines for nursing- and healthcare-associated pneumonia (NHCAP) were developed for pneumonia caused by drug-resistant bacteria and pneumonia in elderly patients, particularly aspiration pneumonia. The identification of pathogenic bacteria and implementation of efforts to prevent the recurrence of aspiration pneumonia are very important in clinical practice. This study examined the extent to which clinicians have established bacteriological testing and recurrence prevention efforts for NHCAP and aspiration pneumonia. Methods Questionnaire surveys were mailed to the heads of internal medicine and respiratory medicine departments at 2490 Japanese hospitals. The questionnaire evaluated bacteriological testing for NHCAP or aspiration pneumonia and prevention of the recurrence of aspiration pneumonia. Results A total of 350 hospitals responded. These hospitals were grouped on the basis of whether a pulmonologist provided medical care for aspiration pneumonia and whether the hospital employed an infectious disease specialist. For hospitals in which pulmonologists treated aspiration pneumonia, the response rates for "is done in nearly all cases" were 70.0%, 84.7%, 31.6%, and 48.9% for sputum Gram Staining, sputum culture tests, blood culture tests, and pneumococcal vaccination, respectively. In hospitals that employed an infectious disease specialist, the response rates for "is done in nearly all cases" were 72.8% and 41.3% for sputum Gram Staining and blood culture tests, respectively. Recurrence prevention for aspiration pneumonia (other than pneumococcal vaccination) was not actively implemented. Conclusions Sputum Gram Staining, sputum culture tests, and other bacteriological tests were implemented quite actively. However, physicians who treat aspiration pneumonia should implement efforts to prevent pneumonia recurrence more actively.
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Gram Staining of blood cultures for clostridium perfringens
QJM: An International Journal of Medicine, 2016Co-Authors: Tsuneaki Kenzaka, Takeshi UeshimoAbstract:A 75-year-old man with chronic heart failure and chronic obstructive pulmonary disease visited our emergency outpatient unit because of fever of 38 °C and dyspnea. His vital signs were as follows: blood pressure 150/68 mmHg; pulse 103 beats/min, regular; respiratory rate 26 breaths/min; temperature 38.6 °C; and SpO2 90% (without supplemental oxygen). A systolic murmur (Levine scale 2/6) was heard at the apex. Coarse crackles were auscultated over the lung bases bilaterally. There were no other abnormal physical findings. Blood tests showed a white blood cell count of 12.8 × 109/L and C-reactive protein of …
Isabel Sanfeliu - One of the best experts on this subject based on the ideXlab platform.
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direct identification of ruminococcus gnavus by matrix assisted laser desorption ionization time of flight mass spectrometry maldi tof ms on a positive anaerobic blood culture bottle
Anaerobe, 2018Co-Authors: Dionisia Fontanals, Alex Larruzea, Isabel SanfeliuAbstract:Abstract We report a case of bloodstream infection with the anaerobic bacterium Ruminococcus gnavus (R. gnavus), associated with intestinal perforation in a patient undergoing chemotherapy for multiple myeloma and cancer of the sigmoid colon. Gram Staining of positive anaerobic blood cultures revealed both diplococci and short chains of Gram-positive cocci. MALDI-TOF MS done directly on the blood culture bottle identified the bacterium as R. gnavus, and 16S rRNA gene sequencing confirmed the identification.
