The Experts below are selected from a list of 129726 Experts worldwide ranked by ideXlab platform
Shinya Nagamatsu - One of the best experts on this subject based on the ideXlab platform.
-
imaging exocytosis of single insulin secretory Granules with evanescent wave microscopy distinct behavior of granule motion in biphasic insulin release
Journal of Biological Chemistry, 2002Co-Authors: Mica Oharaimaizumi, Yoko Nakamichi, Toshiaki Tanaka, Hitoshi Ishida, Shinya NagamatsuAbstract:To study insulin exocytosis by monitoring the single insulin secretory granule motion, evanescent wave microscopy was used to quantitatively analyze the final stage of insulin exocytosis with biphasic release. Green fluorescent protein-tagged insulin transfected in MIN6 beta cells was packed in insulin secretory Granules, which appeared to preferentially dock to the plasma membrane. Upon fusion evoked by secretagogues, evanescent wave microscopy revealed that fluorescence of green fluorescent protein-tagged insulin brightened, spread (within 300 ms), and then vanished. Under KCl stimulation, which represents the 1st phase of release, the successive fusion events were seen mostly from previously docked Granules for the first minute, followed by the recruitment of new Granules to the plasmalemmal docking sites. Stimulation with glucose, in contrast, caused the fusion events from previously docked Granules for the first 120 s, thereafter a continuous fusion (2nd phase of release) was observed over 10 min mostly from newly recruited Granules that progressively accumulated on the plasma membrane. Thus, our data revealed the distinct behavior of the insulin granule motion during the 1st and 2nd phase of release.
-
imaging exocytosis of single insulin secretory Granules with evanescent wave microscopy distinct behavior of granule motion in biphasic insulin release
Journal of Biological Chemistry, 2002Co-Authors: Mica Oharaimaizumi, Yoko Nakamichi, Toshiaki Tanaka, Hitoshi Ishida, Shinya NagamatsuAbstract:To study insulin exocytosis by monitoring the single insulin secretory granule motion, evanescent wave microscopy was used to quantitatively analyze the final stage of insulin exocytosis with biphasic release. Green fluorescent protein-tagged insulin transfected in MIN6 β cells was packed in insulin secretory Granules, which appeared to preferentially dock to the plasma membrane. Upon fusion evoked by secretagogues, evanescent wave microscopy revealed that fluorescence of green fluorescent protein-tagged insulin brightened, spread (within 300 ms), and then vanished. Under KCl stimulation, which represents the 1st phase of release, the successive fusion events were seen mostly from previously docked Granules for the first minute, followed by the recruitment of new Granules to the plasmalemmal docking sites. Stimulation with glucose, in contrast, caused the fusion events from previously docked Granules for the first 120 s, thereafter a continuous fusion (2nd phase of release) was observed over 10 min mostly from newly recruited Granules that progressively accumulated on the plasma membrane. Thus, our data revealed the distinct behavior of the insulin granule motion during the 1st and 2nd phase of release.
Mica Oharaimaizumi - One of the best experts on this subject based on the ideXlab platform.
-
imaging exocytosis of single insulin secretory Granules with evanescent wave microscopy distinct behavior of granule motion in biphasic insulin release
Journal of Biological Chemistry, 2002Co-Authors: Mica Oharaimaizumi, Yoko Nakamichi, Toshiaki Tanaka, Hitoshi Ishida, Shinya NagamatsuAbstract:To study insulin exocytosis by monitoring the single insulin secretory granule motion, evanescent wave microscopy was used to quantitatively analyze the final stage of insulin exocytosis with biphasic release. Green fluorescent protein-tagged insulin transfected in MIN6 beta cells was packed in insulin secretory Granules, which appeared to preferentially dock to the plasma membrane. Upon fusion evoked by secretagogues, evanescent wave microscopy revealed that fluorescence of green fluorescent protein-tagged insulin brightened, spread (within 300 ms), and then vanished. Under KCl stimulation, which represents the 1st phase of release, the successive fusion events were seen mostly from previously docked Granules for the first minute, followed by the recruitment of new Granules to the plasmalemmal docking sites. Stimulation with glucose, in contrast, caused the fusion events from previously docked Granules for the first 120 s, thereafter a continuous fusion (2nd phase of release) was observed over 10 min mostly from newly recruited Granules that progressively accumulated on the plasma membrane. Thus, our data revealed the distinct behavior of the insulin granule motion during the 1st and 2nd phase of release.
-
imaging exocytosis of single insulin secretory Granules with evanescent wave microscopy distinct behavior of granule motion in biphasic insulin release
Journal of Biological Chemistry, 2002Co-Authors: Mica Oharaimaizumi, Yoko Nakamichi, Toshiaki Tanaka, Hitoshi Ishida, Shinya NagamatsuAbstract:To study insulin exocytosis by monitoring the single insulin secretory granule motion, evanescent wave microscopy was used to quantitatively analyze the final stage of insulin exocytosis with biphasic release. Green fluorescent protein-tagged insulin transfected in MIN6 β cells was packed in insulin secretory Granules, which appeared to preferentially dock to the plasma membrane. Upon fusion evoked by secretagogues, evanescent wave microscopy revealed that fluorescence of green fluorescent protein-tagged insulin brightened, spread (within 300 ms), and then vanished. Under KCl stimulation, which represents the 1st phase of release, the successive fusion events were seen mostly from previously docked Granules for the first minute, followed by the recruitment of new Granules to the plasmalemmal docking sites. Stimulation with glucose, in contrast, caused the fusion events from previously docked Granules for the first 120 s, thereafter a continuous fusion (2nd phase of release) was observed over 10 min mostly from newly recruited Granules that progressively accumulated on the plasma membrane. Thus, our data revealed the distinct behavior of the insulin granule motion during the 1st and 2nd phase of release.
Garth J. S. Cooper - One of the best experts on this subject based on the ideXlab platform.
-
Proteins Associated with Immunopurified Granules from a Model Pancreatic Islet β-Cell System: Proteomic Snapshot of an Endocrine Secretory Granule
Journal of proteome research, 2009Co-Authors: Anthony J. R. Hickey, Joshua Bradley, Gretchen L. Skea, Martin Middleditch, Christina M. Buchanan, Anthony R. J. Phillips, Garth J. S. CooperAbstract:beta-Cell Granules contain proteins involved in fuel regulation, which when altered, contribute to metabolic disorders including diabetes mellitus. We analyzed proteins present in purified Granules from the INS-1E beta-cell model. Fifty-one component proteins were identified by LC-MS/MS including hormones, granins, protein processing components, cellular trafficking components, enzymes implicated in cellular metabolism and chaperone proteins. These findings may increase understanding of granule secretion and the processes leading to protein aggregation and beta-cell death in type-2 diabetes.
-
proteins associated with immunopurified Granules from a model pancreatic islet beta cell system proteomic snapshot of an endocrine secretory granule
Journal of Proteome Research, 2009Co-Authors: Anthony J. R. Hickey, Joshua Bradley, Gretchen L. Skea, Martin Middleditch, Christina M. Buchanan, Anthony R. J. Phillips, Garth J. S. CooperAbstract:beta-Cell Granules contain proteins involved in fuel regulation, which when altered, contribute to metabolic disorders including diabetes mellitus. We analyzed proteins present in purified Granules from the INS-1E beta-cell model. Fifty-one component proteins were identified by LC-MS/MS including hormones, granins, protein processing components, cellular trafficking components, enzymes implicated in cellular metabolism and chaperone proteins. These findings may increase understanding of granule secretion and the processes leading to protein aggregation and beta-cell death in type-2 diabetes.
Hitoshi Ishida - One of the best experts on this subject based on the ideXlab platform.
-
imaging exocytosis of single insulin secretory Granules with evanescent wave microscopy distinct behavior of granule motion in biphasic insulin release
Journal of Biological Chemistry, 2002Co-Authors: Mica Oharaimaizumi, Yoko Nakamichi, Toshiaki Tanaka, Hitoshi Ishida, Shinya NagamatsuAbstract:To study insulin exocytosis by monitoring the single insulin secretory granule motion, evanescent wave microscopy was used to quantitatively analyze the final stage of insulin exocytosis with biphasic release. Green fluorescent protein-tagged insulin transfected in MIN6 beta cells was packed in insulin secretory Granules, which appeared to preferentially dock to the plasma membrane. Upon fusion evoked by secretagogues, evanescent wave microscopy revealed that fluorescence of green fluorescent protein-tagged insulin brightened, spread (within 300 ms), and then vanished. Under KCl stimulation, which represents the 1st phase of release, the successive fusion events were seen mostly from previously docked Granules for the first minute, followed by the recruitment of new Granules to the plasmalemmal docking sites. Stimulation with glucose, in contrast, caused the fusion events from previously docked Granules for the first 120 s, thereafter a continuous fusion (2nd phase of release) was observed over 10 min mostly from newly recruited Granules that progressively accumulated on the plasma membrane. Thus, our data revealed the distinct behavior of the insulin granule motion during the 1st and 2nd phase of release.
-
imaging exocytosis of single insulin secretory Granules with evanescent wave microscopy distinct behavior of granule motion in biphasic insulin release
Journal of Biological Chemistry, 2002Co-Authors: Mica Oharaimaizumi, Yoko Nakamichi, Toshiaki Tanaka, Hitoshi Ishida, Shinya NagamatsuAbstract:To study insulin exocytosis by monitoring the single insulin secretory granule motion, evanescent wave microscopy was used to quantitatively analyze the final stage of insulin exocytosis with biphasic release. Green fluorescent protein-tagged insulin transfected in MIN6 β cells was packed in insulin secretory Granules, which appeared to preferentially dock to the plasma membrane. Upon fusion evoked by secretagogues, evanescent wave microscopy revealed that fluorescence of green fluorescent protein-tagged insulin brightened, spread (within 300 ms), and then vanished. Under KCl stimulation, which represents the 1st phase of release, the successive fusion events were seen mostly from previously docked Granules for the first minute, followed by the recruitment of new Granules to the plasmalemmal docking sites. Stimulation with glucose, in contrast, caused the fusion events from previously docked Granules for the first 120 s, thereafter a continuous fusion (2nd phase of release) was observed over 10 min mostly from newly recruited Granules that progressively accumulated on the plasma membrane. Thus, our data revealed the distinct behavior of the insulin granule motion during the 1st and 2nd phase of release.
Toshiaki Tanaka - One of the best experts on this subject based on the ideXlab platform.
-
imaging exocytosis of single insulin secretory Granules with evanescent wave microscopy distinct behavior of granule motion in biphasic insulin release
Journal of Biological Chemistry, 2002Co-Authors: Mica Oharaimaizumi, Yoko Nakamichi, Toshiaki Tanaka, Hitoshi Ishida, Shinya NagamatsuAbstract:To study insulin exocytosis by monitoring the single insulin secretory granule motion, evanescent wave microscopy was used to quantitatively analyze the final stage of insulin exocytosis with biphasic release. Green fluorescent protein-tagged insulin transfected in MIN6 beta cells was packed in insulin secretory Granules, which appeared to preferentially dock to the plasma membrane. Upon fusion evoked by secretagogues, evanescent wave microscopy revealed that fluorescence of green fluorescent protein-tagged insulin brightened, spread (within 300 ms), and then vanished. Under KCl stimulation, which represents the 1st phase of release, the successive fusion events were seen mostly from previously docked Granules for the first minute, followed by the recruitment of new Granules to the plasmalemmal docking sites. Stimulation with glucose, in contrast, caused the fusion events from previously docked Granules for the first 120 s, thereafter a continuous fusion (2nd phase of release) was observed over 10 min mostly from newly recruited Granules that progressively accumulated on the plasma membrane. Thus, our data revealed the distinct behavior of the insulin granule motion during the 1st and 2nd phase of release.
-
imaging exocytosis of single insulin secretory Granules with evanescent wave microscopy distinct behavior of granule motion in biphasic insulin release
Journal of Biological Chemistry, 2002Co-Authors: Mica Oharaimaizumi, Yoko Nakamichi, Toshiaki Tanaka, Hitoshi Ishida, Shinya NagamatsuAbstract:To study insulin exocytosis by monitoring the single insulin secretory granule motion, evanescent wave microscopy was used to quantitatively analyze the final stage of insulin exocytosis with biphasic release. Green fluorescent protein-tagged insulin transfected in MIN6 β cells was packed in insulin secretory Granules, which appeared to preferentially dock to the plasma membrane. Upon fusion evoked by secretagogues, evanescent wave microscopy revealed that fluorescence of green fluorescent protein-tagged insulin brightened, spread (within 300 ms), and then vanished. Under KCl stimulation, which represents the 1st phase of release, the successive fusion events were seen mostly from previously docked Granules for the first minute, followed by the recruitment of new Granules to the plasmalemmal docking sites. Stimulation with glucose, in contrast, caused the fusion events from previously docked Granules for the first 120 s, thereafter a continuous fusion (2nd phase of release) was observed over 10 min mostly from newly recruited Granules that progressively accumulated on the plasma membrane. Thus, our data revealed the distinct behavior of the insulin granule motion during the 1st and 2nd phase of release.
