The Experts below are selected from a list of 1341 Experts worldwide ranked by ideXlab platform
Nese Imeryuz - One of the best experts on this subject based on the ideXlab platform.
-
Treatment of Hermansky-Pudlak syndrome Associated Granulomatous Colitis with anti-TNF agents: case series and review of literature.
European journal of gastroenterology & hepatology, 2019Co-Authors: Coskun Ozer Demirtas, Yesim Ozen Alahdab, Haluk Tarik Kani, Ozlen Atug, Nese ImeryuzAbstract:Hermansky-Pudlak syndrome is a rare syndrome characterized by bleeding diathesis due to platelet dysfunction, oculocutaneous albinism and other systemic involvements. Granulomatous Colitis may occur in the disease course and have similarities with Crohn's disease. Herein, we present four cases with Hermansky-Pudlak syndrome associated Colitis with the longest follow-up period having various responses to different anti-TNF agents. Four patients with Hermansky-Pudlak syndrome Colitis were started on anti-TNF agents between 2008 and 2013. After a mean follow-up period of 7.5 years, two of four patients exhibited a significant improvement in symptoms, whereas the other two experienced undesirable disease course. Although having many similarities with Crohn's disease; Hermansky-Pudlak syndrome Colitis appears to have lower anti-TNF response rates. Pathophysiological differences need to be enlightened to find more appropriate therapeutic targets for Hermansky-Pudlak syndrome Colitis.
Hiroshi Takemori - One of the best experts on this subject based on the ideXlab platform.
-
Simple chronic Colitis model using hypopigmented mice with a Hermansky-Pudlak syndrome 5 gene mutation.
Pigment cell & melanoma research, 2016Co-Authors: Yumi Itoh, Yasuo Nagaoka, Yoshio Katakura, Hidehisa Kawahara, Hiroshi TakemoriAbstract:Pigmentation in mammals is important for protection of skin and eyes from ultraviolet radiation. Dysregulation of pigmentation is often associated with other conditions that are not directly linked to pigmentation. Here, we isolated spontaneously occurring hypopigmented mice that occasionally experienced severe diarrhea during lactation. Treatment of these mice with dextran sulfate sodium salt, a conventional method to induce acute Colitis, caused chronic diarrhea with Granulomatous Colitis. Gene mapping and sequencing revealed that the mice had a nonsense mutation in the Hermansky-Pudlak syndrome (Hps)5 gene. As some HPS patients can develop Granulomatous Colitis, the simple induction of chronic Colitis in spontaneously mutated Hps5-deficient mice may become an invaluable model for exploring treatment options in patients with HPS as well as other patients with inflammatory bowel disease.
F Daum - One of the best experts on this subject based on the ideXlab platform.
-
Hermansky-Pudlak syndrome with Granulomatous Colitis in children
The Journal of pediatrics, 1991Co-Authors: Robby Mahadeo, James Markowitz, Stanley E. Fisher, F DaumAbstract:in the lungs in cirrhosis of the liver and lung spider nevi. N Engl J Med 1966;274:291-8. 5. Davis HH, Schwartz D J, LeFrak SS, Susman H, Schainker BA. Alveolar-capillary oxygen disequilibrium in hepatic cirrhosis. Chest 1978;73:507-11. 6. Genovesi MG, Tierney DF, Taplan GV, Eisenberg H. An intravenous radionuclide method to evaluate hypoxemia caused by abnormal alveolar vessels: limitations of conventional techniques. Am Rev Respir Dis 1976;l 14:59-65. 7. Hind CRK, Wong CM. Detection of pulmonary arteriovenous fistulae in patient with cirrhosis by contrast 2D echocardiography. Gut 1981;22:1042-5. 8. Shub C, Tajik A J, Seward JB, Dines DE. Detecting intrapulmonary right-to-left shunt with contrast echocardiography: observations in a patient with diffuse pulmonary arteriovenous fistulas. Mayo Clin Proc 1976;51:81-4. 9. Van Thiel DH, Schade RR, Gavaler JB, et al. Medical aspects of liver transplantation. Hepatology 1984;4(suppl):79-83.
Kenneth W. Simpson - One of the best experts on this subject based on the ideXlab platform.
-
Escherichia coli-associated Granulomatous Colitis in dogs treated according to antimicrobial susceptibility profiling.
Journal of veterinary internal medicine, 2020Co-Authors: Alison C Manchester, Belgin Dogan, Yongli Guo, Kenneth W. SimpsonAbstract:Eradication of intramucosal Escherichia coli correlates with remission of periodic acid-Schiff-positive E coli-associated Granulomatous Colitis (GC). Treatment failures attributed to multidrug resistant (MDR) bacteria necessitate alternative approaches. Determine clinical outcome of E coli-associated GC in dogs treated based on antimicrobial susceptibility profiling and characterize E coli phylogeny and resistance mechanisms. Twenty Boxers and 4 French Bulldogs with E coli-associated GC. Culture, antimicrobial susceptibility profiling, and molecular characterization of E coli were performed and response to treatment was evaluated. Initial biopsy sample culture yielded fluoroquinolone-sensitive (FQ-S) E coli from 9/24 dogs and fluoroquinolone-resistant (FQ-R) E coli from 15/24. All but 1 FQ-R E coli were MDR with susceptibility to macrophage-penetrating antimicrobials restricted to carbapenems in 13/15 dogs. Of 22/24 treated based on susceptibility profiling, 8/9 FQ-S dogs had complete initial clinical response (CR) during fluoroquinolone (FQ) treatment, whereas 9/13 FQ-R dogs had complete or partial response (PR) during meropenem or doxycycline treatment. In 5/9 FQ-S and 12/13 FQ-R dogs with follow-up ≥3 months, CR was sustained in 5/5 FQ-S (median, 25 months; range, 4-46) whereas 6/12 FQ-R had long-term CR (median, 59 months; range 15-102), 4/12 PR (median, 19 months; range, 5-65), and 2/12 had no response (NR). Four dogs with long-term follow-up died within 4 years of diagnosis, including 2 euthanized for refractory Colitis. Escherichia coli were genetically diverse. Fluoroquinolone resistance was associated with mutations in gyrA and parC, with plasmid-mediated resistance less common. Antimicrobial treatment guided by susceptibility profiling was associated with positive long-term outcomes in >80% of cases. Fluoroquinolone-resistance was widespread and not clonal. Further study is required to optimize treatment for dogs with MDR E coli-associated GC. © 2020 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC. on behalf of the American College of Veterinary Internal Medicine.
-
escherichia coli associated Granulomatous Colitis in dogs treated according to antimicrobial susceptibility profiling
Journal of Veterinary Internal Medicine, 2020Co-Authors: Alison C Manchester, Belgin Dogan, Yongli Guo, Kenneth W. SimpsonAbstract:Background Eradication of intramucosal Escherichia coli correlates with remission of periodic acid-Schiff-positive E coli-associated Granulomatous Colitis (GC). Treatment failures attributed to multidrug resistant (MDR) bacteria necessitate alternative approaches. Hypothesis/objectives Determine clinical outcome of E coli-associated GC in dogs treated based on antimicrobial susceptibility profiling and characterize E coli phylogeny and resistance mechanisms. Animals Twenty Boxers and 4 French Bulldogs with E coli-associated GC. Methods Culture, antimicrobial susceptibility profiling, and molecular characterization of E coli were performed and response to treatment was evaluated. Results Initial biopsy sample culture yielded fluoroquinolone-sensitive (FQ-S) E coli from 9/24 dogs and fluoroquinolone-resistant (FQ-R) E coli from 15/24. All but 1 FQ-R E coli were MDR with susceptibility to macrophage-penetrating antimicrobials restricted to carbapenems in 13/15 dogs. Of 22/24 treated based on susceptibility profiling, 8/9 FQ-S dogs had complete initial clinical response (CR) during fluoroquinolone (FQ) treatment, whereas 9/13 FQ-R dogs had complete or partial response (PR) during meropenem or doxycycline treatment. In 5/9 FQ-S and 12/13 FQ-R dogs with follow-up ≥3 months, CR was sustained in 5/5 FQ-S (median, 25 months; range, 4-46) whereas 6/12 FQ-R had long-term CR (median, 59 months; range 15-102), 4/12 PR (median, 19 months; range, 5-65), and 2/12 had no response (NR). Four dogs with long-term follow-up died within 4 years of diagnosis, including 2 euthanized for refractory Colitis. Escherichia coli were genetically diverse. Fluoroquinolone resistance was associated with mutations in gyrA and parC, with plasmid-mediated resistance less common. Conclusions and clinical importance Antimicrobial treatment guided by susceptibility profiling was associated with positive long-term outcomes in >80% of cases. Fluoroquinolone-resistance was widespread and not clonal. Further study is required to optimize treatment for dogs with MDR E coli-associated GC.
-
Molecular and Phenotypic Characterization of Escherichia coli Associated with Granulomatous Colitis of Boxer Dogs.
Antibiotics (Basel Switzerland), 2020Co-Authors: Belgin Dogan, Shiying Zhang, Sarah E Kalla, Esra I Dogan, Cindy Guo, Chelston Ang, Kenneth W. SimpsonAbstract:Invasive Escherichia coli is causally associated with Granulomatous Colitis (GC) of Boxer dogs and French Bulldogs. The virulence determinants of GC E. coli are unclear. E. coli isolated from 16 GC (36 strains) and 17 healthy control (HC: 33 strains) dogs were diverse in phylogeny, genotype, and serotype and lacked diarrheagenic genes. Genes encoding type II (gsp), IV (traC), and VI (hcp) secretion systems, long polar fimbriae (lpfA154/141), and iron acquisition (fyuA, chuA) were frequent in GC and HC. E. coli from 14/15 GC and 10/11 HC invaded Caco-2 better than non-pathogenic E. coli strain DH5α, with invasion correlated with motility and presence of chuA and colV. E. coli from all GC and 10/11 HC survived better than DH5α in J774 macrophages, with adherent-invasive E. coli (AIEC) in 60% GC and 73% HC. AIEC replicated in monocyte derived macrophages from a GC Boxer with CD48/SLAM risk haplotype but not the HC. Fluroquinolone resistant E. coli were less motile and invasive than fluoroquinolone sensitive (p < 0.05), and only 1/8 resistant strains met criteria for AIEC. In conclusion GC E. coli are diverse, resemble extraintestinal pathogenic E. coli (ExPEC), including AIEC, and can replicate in GC-susceptible macrophages. They are likely resident pathosymbionts that can opportunistically persist within macrophages of a GC-susceptible dog.
-
Granulomatous Colitis of boxer dogs.
The Veterinary clinics of North America. Small animal practice, 2011Co-Authors: Melanie Craven, Caroline Mansfield, Kenneth W. SimpsonAbstract:Granulomatous Colitis (GC) is a rare, breed-specific inflammatory bowel disease of young Boxer dogs. GC has been refractory to treatment and associated with high mortality rates, but culture-independent molecular analysis has transformed therapy and prognosis by uncovering a correlation between GC and Escherichia coli invasion within colonic mucosal macrophages. GC-associated invasive E coli are similar to a newly identified E coli pathotype, “adherent and invasive E coli,” that are increasingly associated with Crohn's disease in humans. Successful treatment of GC requires antimicrobials that are effective against E coli and penetrate intracellularly. Enrofloxacin is widely regarded as the antibiotic of choice.
-
Adherent and Invasive Escherichia coli Is Associated with Granulomatous Colitis in Boxer Dogs
Infection and immunity, 2006Co-Authors: Kenneth W. Simpson, Belgin Dogan, Patrick L. Mcdonough, Mark Rishniw, Richard E. Goldstein, Suzanne Klaessig, Alexander J. German, Robin M. Yates, David G. Russell, Susan E. JohnsonAbstract:The mucosa-associated microflora is increasingly considered to play a pivotal role in the pathogenesis of inflammatory bowel disease. This study explored the possibility that an abnormal mucosal flora is involved in the etiopathogenesis of Granulomatous Colitis of Boxer dogs (GCB). Colonic biopsy samples from affected dogs (n 13) and controls (n 38) were examined by fluorescent in situ hybridization (FISH) with a eubacterial 16S rRNA probe. Culture, 16S ribosomal DNA sequencing, and histochemistry were used to guide subsequent FISH. GCB-associated Escherichia coli isolates were evaluated for their ability to invade and persist in cultured epithelial cells and macrophages as well as for serotype, phylogenetic group, genome size, overall genotype, and presence of virulence genes. Intramucosal gram-negative coccobacilli were present in 100% of GCB samples but not controls. Invasive bacteria hybridized with FISH probes to E. coli. Three of four GCB-associated E. coli isolates adhered to, invaded, and replicated within cultured epithelial cells. Invasion triggered a “splash”-type response, was decreased by cytochalasin D, genistein, colchicine, and wortmannin, and paralleled the behavior of the Crohn’s disease-associated strain E. coli LF 82. GCB E. coli and LF 82 were diverse in serotype and overall genotype but similar in phylogeny (B2 and D), in virulence gene profiles (fyuA, irp1, irp2, chuA, fepC, ibeA, kpsMII, iss), in having a larger genome size than commensal E. coli, and in the presence of novel multilocus sequence types. We conclude that GCB is associated with selective intramucosal colonization by E. coli. E. coli strains associated with GCB and Crohn’s disease have an adherent and invasive phenotype and novel multilocus sequence types and resemble E. coli associated with extraintestinal disease in phylogeny and virulence gene profile.
Coskun Ozer Demirtas - One of the best experts on this subject based on the ideXlab platform.
-
Treatment of Hermansky-Pudlak syndrome Associated Granulomatous Colitis with anti-TNF agents: case series and review of literature.
European journal of gastroenterology & hepatology, 2019Co-Authors: Coskun Ozer Demirtas, Yesim Ozen Alahdab, Haluk Tarik Kani, Ozlen Atug, Nese ImeryuzAbstract:Hermansky-Pudlak syndrome is a rare syndrome characterized by bleeding diathesis due to platelet dysfunction, oculocutaneous albinism and other systemic involvements. Granulomatous Colitis may occur in the disease course and have similarities with Crohn's disease. Herein, we present four cases with Hermansky-Pudlak syndrome associated Colitis with the longest follow-up period having various responses to different anti-TNF agents. Four patients with Hermansky-Pudlak syndrome Colitis were started on anti-TNF agents between 2008 and 2013. After a mean follow-up period of 7.5 years, two of four patients exhibited a significant improvement in symptoms, whereas the other two experienced undesirable disease course. Although having many similarities with Crohn's disease; Hermansky-Pudlak syndrome Colitis appears to have lower anti-TNF response rates. Pathophysiological differences need to be enlightened to find more appropriate therapeutic targets for Hermansky-Pudlak syndrome Colitis.
