The Experts below are selected from a list of 699 Experts worldwide ranked by ideXlab platform
Joel Fleurence - One of the best experts on this subject based on the ideXlab platform.
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can the european abalone haliotis tuberculata survive on an invasive algae a comparison of the nutritional value of the introduced Grateloupia turuturu and the native palmaria palmata for the commercial european abalone industry
Journal of Applied Phycology, 2016Co-Authors: Nuria Garciabueno, Vincent Turpin, Justine Dumay, Michele Morancais, Bruno Cognie, Mireille Amat, Jeanmarie Pedron, Arnaldo Marin Atucha, Joel FleurenceAbstract:The suitability of two red algae species, the introduced Grateloupia turuturu and the native Palmaria palmata, as feed for the culture of the European abalone Haliotis tuberculata, was compared over a 5-month period. Three experimental diets were tested: (1) P. palmata, (2) G. turuturu, and (3) a mixed diet of P. palmata and G. turuturu (1:1). Biochemical composition (proteins, carbohydrates, lipids, ashes) of algae was measured. No mortality was observed during the time of the experiment in any of the treatments. Growth in length and weight was higher for abalone fed with P. palmata and the mixed diet. Abalone exhibited a preference for P. palmata and showed the highest weight gain with this macroalgae (107.8 ± 7.2 %). Grateloupia turuturu disintegrated faster in abalone rearing conditions and was not suitable for significant growth of H. tuberculata in a monospecific diet. However, when it is provided in a mixed diet, abalone seem to show a higher lipid content than when fed a P. palmata monospecific diet. The invasive G. turuturu could be an interesting food supplement for European abalone.
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Seasonal variation in the antivibrio activity of two organic extracts from two red seaweed:
'EDP Sciences', 2016Co-Authors: Nuria García-bueno, Thomas Guerin, Yves Francois Pouchus, Christine Paillard, Vincent Turpin, Priscilla Decottignies, Justine Dumay, Valérie Stiger-pouvreau, Arnaldo Aitor Marín-atucha, Joel FleurenceAbstract:The wide polarity range and highly polar compounds of two selected red seaweed, Grateloupia turuturu and Palmaria palmata were extracted using two different types of solvent, dichloromethane/methanol and methanol/water. Monthly in vitro antibacterial activities were studied using the microplate method against the marine bacteria Vibrio harveyi strain ORM4, known to infect abalone. Inhibition, slowdown and delay of Vibrio harveyi growth were investigated. Polar compounds of seaweed showed an activity against the abalone pathogen. The best activity was recorded from P. palmata collected in spring, with an inhibition of 7.9% of the bacterial growth. Preliminary 1H NMR profiles identified the differences between the extracts
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ultrasound assisted extraction of r phycoerythrin from Grateloupia turuturu with and without enzyme addition
Algal Research-Biomass Biofuels and Bioproducts, 2015Co-Authors: Joel Fleurence, Justine Dumay, Cecile Le Guillard, Claire Donnaymoreno, Sandrine Bruzac, Jeanyves Ragon, Jeanpascal BergeAbstract:Abstract The aim of this study was to compare two processes for the extraction of R-phycoerythrin (R-PE) from the red seaweed Grateloupia turuturu : ultrasound-assisted extraction (UAE) and ultrasound-assisted enzymatic hydrolysis (UAEH). Process efficiencies were both evaluated by the yield of R-PE extraction and by the level of liquefaction. Experiments were conducted at 40 and 22 °C, for 6 h, using an enzymatic cocktail and an original ultrasonic flow-through reactor. R-PE appeared very sensitive to temperature, thus 22 °C is strongly recommended for its extraction by UAEH or UAE. However, the higher processing temperature (40 °C) clearly increased the extraction of water-soluble compounds (up to 91% of liquefaction). These two new processes are thus promising alternatives for the extraction of water-soluble components including R-PE, from wet seaweeds, with extraction yields at least similar to conventional solid–liquid extraction.
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one step purification of r phycoerythrin from the red edible seaweed Grateloupia turuturu
Journal of Chromatography B, 2015Co-Authors: Mathilde Munier, Justine Dumay, Michele Morancais, Pascal Jaouen, Joel FleurenceAbstract:A one-step chromatographic method for the purification of R-phycoerythrin (R-PE) of Grateloupia turuturu Yamada is described. Native R-PE was obtained with a purity index of 2.89 and a recovery yield of 27% using DEAE-Sepharose Fast Flow chromatography with a three-step increase in ionic strength. The analysis by SDS electrophoresis showed a broad band between 18 and 21kDa in size corresponding to subunits α and β and a low intensity band of 29kDa corresponding to the γ subunit. Two forms of R-PE were identified by gel filtration chromatography: a native form with a molecular weight of 260±5kDa and a dissociated form with a molecular weight of 60±2kDa. The native form presented the characteristic absorption spectrum of R-PE with three absorbance maxima at 498, 540 and 565nm, whereas the dissociated form presented only the 498 and 540nm peaks. Moreover, the two forms displayed two different fluorescence maxima.
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physicochemical factors affecting the stability of two pigments r phycoerythrin of Grateloupia turuturu and b phycoerythrin of porphyridium cruentum
Food Chemistry, 2014Co-Authors: Mathilde Munier, Justine Dumay, Michele Morancais, Pascal Jaouen, Alva Wijaya, Joel FleurenceAbstract:Abstract Phycoerythrin is a major light-harvesting pigment of red algae, which could be used as a natural dye in foods. The stability of R-phycoerythrin of Grateloupia turuturu and B-phycoerythrin of Porphyridium cruentum in relation to different light exposure times, pHs, and temperatures was studied. Regarding the light exposure time, after 48 h, the reduction in concentrations of B-phycoerythrin and R-phycoerythrin were 30 ± 2.4% and 70 ± 1%, respectively. Phycoerythrins presented good stability from pH 4 to 10. At pH 2, the reduction in concentration was 90 ± 4% for B-phycoerythrin and 40 ± 2.5% for R-phycoerythrin while, at pH 12, the phycoerythrins were degraded. Phycoerythrins showed good stability toward temperature, up to 40 °C. At 60 °C, the reduction in concentrations of B-phycoerythrin and R-phycoerythrin were 50 ± 3.4% and 70 ± 0.18%, respectively. Moreover, the best conditions of storage (−20 °C) were determined.
Nuria Garciabueno - One of the best experts on this subject based on the ideXlab platform.
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can the european abalone haliotis tuberculata survive on an invasive algae a comparison of the nutritional value of the introduced Grateloupia turuturu and the native palmaria palmata for the commercial european abalone industry
Journal of Applied Phycology, 2016Co-Authors: Nuria Garciabueno, Vincent Turpin, Justine Dumay, Michele Morancais, Bruno Cognie, Mireille Amat, Jeanmarie Pedron, Arnaldo Marin Atucha, Joel FleurenceAbstract:The suitability of two red algae species, the introduced Grateloupia turuturu and the native Palmaria palmata, as feed for the culture of the European abalone Haliotis tuberculata, was compared over a 5-month period. Three experimental diets were tested: (1) P. palmata, (2) G. turuturu, and (3) a mixed diet of P. palmata and G. turuturu (1:1). Biochemical composition (proteins, carbohydrates, lipids, ashes) of algae was measured. No mortality was observed during the time of the experiment in any of the treatments. Growth in length and weight was higher for abalone fed with P. palmata and the mixed diet. Abalone exhibited a preference for P. palmata and showed the highest weight gain with this macroalgae (107.8 ± 7.2 %). Grateloupia turuturu disintegrated faster in abalone rearing conditions and was not suitable for significant growth of H. tuberculata in a monospecific diet. However, when it is provided in a mixed diet, abalone seem to show a higher lipid content than when fed a P. palmata monospecific diet. The invasive G. turuturu could be an interesting food supplement for European abalone.
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seasonal variation in the antivibrio activity of two organic extracts from two red seaweed palmaria palmata and the introduced Grateloupia turuturu against the abalone pathogen vibrio harveyi
Aquatic Living Resources, 2015Co-Authors: Nuria Garciabueno, Thomas Guerin, Yves Francois Pouchus, Christine Paillard, Vincent Turpin, Justine Dumay, Valerie Stigerpouvreau, Arnaldo Aitor MarinatuchaAbstract:The wide polarity range and highly polar compounds of two selected red seaweed, Grateloupia turuturu and Palmaria palmata were extracted using two different types of solvent, dichloromethane/methanol and methanol/water. Monthly in vitro antibacterial activities were studied using the microplate method against the marine bacteria Vibrio harveyi strain ORM4, known to infect abalone. Inhibition, slowdown and delay of Vibrio harveyi growth were investigated. Polar compounds of seaweed showed an activity against the abalone pathogen. The best activity was recorded from P. palmata collected in spring, with an inhibition of 7.9% of the bacterial growth. Preliminary 1H NMR profiles identified the differences between the extracts.
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seasonal antibacterial activity of two red seaweeds palmaria palmata and Grateloupia turuturu on european abalone pathogen vibrio harveyi
Aquatic Living Resources, 2014Co-Authors: Nuria Garciabueno, Yves Francois Pouchus, Christine Paillard, Vincent Turpin, Priscilla Decottignies, Justine Dumay, Valerie Stigerpouvreau, Nelly Kervarec, Arnaldo Aitor MarinatuchaAbstract:Vibrio harveyi is the main pathogen of the European abalone Haliotis tuberculata, and recently caused important mortalities at the production sites of this marine gastropod in France. In the present work, the monthly an- tibacterial activity of two red seaweed species from the French Atlantic coast, the native Palmaria palmata and the intro- duced Grateloupia turuturu, were investigated against the abalone pathogen Vibrio harveyi strain ORM4. Water-soluble extracts were screened using the microplate method. Grateloupia turuturu showed an antibacterial activity with a max- imal growth inhibition in spring of around 16%. In contrast, Palmaria palmata was inactive, as further growth of the bacteria was observed. Preliminary one-dimensional proton nuclear magnetic-resonance ( 1 H NMR) profiles identified the differences between the two water-soluble extracts.
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seasonal antibacterial activity of two red seaweeds palmaria palmata and Grateloupia turuturu on european abalone pathogen vibrio harveyi
Aquatic Living Resources, 2014Co-Authors: Nuria Garciabueno, Yves Francois Pouchus, Christine Paillard, Vincent Turpin, Priscilla Decottignies, Justine Dumay, Valerie Stigerpouvreau, Nelly Kervarec, Arnaldo Aitor MarinatuchaAbstract:Vibrio harveyi is the main pathogen of the European abalone Haliotis tuberculata, and recently caused important mortalities at the production sites of this marine gastropod in France. In the present work, the monthly an- tibacterial activity of two red seaweed species from the French Atlantic coast, the native Palmaria palmata and the intro- duced Grateloupia turuturu, were investigated against the abalone pathogen Vibrio harveyi strain ORM4. Water-soluble extracts were screened using the microplate method. Grateloupia turuturu showed an antibacterial activity with a max- imal growth inhibition in spring of around 16%. In contrast, Palmaria palmata was inactive, as further growth of the bacteria was observed. Preliminary one-dimensional proton nuclear magnetic-resonance ( 1 H NMR) profiles identified the differences between the two water-soluble extracts.
Justine Dumay - One of the best experts on this subject based on the ideXlab platform.
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can the european abalone haliotis tuberculata survive on an invasive algae a comparison of the nutritional value of the introduced Grateloupia turuturu and the native palmaria palmata for the commercial european abalone industry
Journal of Applied Phycology, 2016Co-Authors: Nuria Garciabueno, Vincent Turpin, Justine Dumay, Michele Morancais, Bruno Cognie, Mireille Amat, Jeanmarie Pedron, Arnaldo Marin Atucha, Joel FleurenceAbstract:The suitability of two red algae species, the introduced Grateloupia turuturu and the native Palmaria palmata, as feed for the culture of the European abalone Haliotis tuberculata, was compared over a 5-month period. Three experimental diets were tested: (1) P. palmata, (2) G. turuturu, and (3) a mixed diet of P. palmata and G. turuturu (1:1). Biochemical composition (proteins, carbohydrates, lipids, ashes) of algae was measured. No mortality was observed during the time of the experiment in any of the treatments. Growth in length and weight was higher for abalone fed with P. palmata and the mixed diet. Abalone exhibited a preference for P. palmata and showed the highest weight gain with this macroalgae (107.8 ± 7.2 %). Grateloupia turuturu disintegrated faster in abalone rearing conditions and was not suitable for significant growth of H. tuberculata in a monospecific diet. However, when it is provided in a mixed diet, abalone seem to show a higher lipid content than when fed a P. palmata monospecific diet. The invasive G. turuturu could be an interesting food supplement for European abalone.
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Seasonal variation in the antivibrio activity of two organic extracts from two red seaweed:
'EDP Sciences', 2016Co-Authors: Nuria García-bueno, Thomas Guerin, Yves Francois Pouchus, Christine Paillard, Vincent Turpin, Priscilla Decottignies, Justine Dumay, Valérie Stiger-pouvreau, Arnaldo Aitor Marín-atucha, Joel FleurenceAbstract:The wide polarity range and highly polar compounds of two selected red seaweed, Grateloupia turuturu and Palmaria palmata were extracted using two different types of solvent, dichloromethane/methanol and methanol/water. Monthly in vitro antibacterial activities were studied using the microplate method against the marine bacteria Vibrio harveyi strain ORM4, known to infect abalone. Inhibition, slowdown and delay of Vibrio harveyi growth were investigated. Polar compounds of seaweed showed an activity against the abalone pathogen. The best activity was recorded from P. palmata collected in spring, with an inhibition of 7.9% of the bacterial growth. Preliminary 1H NMR profiles identified the differences between the extracts
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ultrasound assisted extraction of r phycoerythrin from Grateloupia turuturu with and without enzyme addition
Algal Research-Biomass Biofuels and Bioproducts, 2015Co-Authors: Joel Fleurence, Justine Dumay, Cecile Le Guillard, Claire Donnaymoreno, Sandrine Bruzac, Jeanyves Ragon, Jeanpascal BergeAbstract:Abstract The aim of this study was to compare two processes for the extraction of R-phycoerythrin (R-PE) from the red seaweed Grateloupia turuturu : ultrasound-assisted extraction (UAE) and ultrasound-assisted enzymatic hydrolysis (UAEH). Process efficiencies were both evaluated by the yield of R-PE extraction and by the level of liquefaction. Experiments were conducted at 40 and 22 °C, for 6 h, using an enzymatic cocktail and an original ultrasonic flow-through reactor. R-PE appeared very sensitive to temperature, thus 22 °C is strongly recommended for its extraction by UAEH or UAE. However, the higher processing temperature (40 °C) clearly increased the extraction of water-soluble compounds (up to 91% of liquefaction). These two new processes are thus promising alternatives for the extraction of water-soluble components including R-PE, from wet seaweeds, with extraction yields at least similar to conventional solid–liquid extraction.
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one step purification of r phycoerythrin from the red edible seaweed Grateloupia turuturu
Journal of Chromatography B, 2015Co-Authors: Mathilde Munier, Justine Dumay, Michele Morancais, Pascal Jaouen, Joel FleurenceAbstract:A one-step chromatographic method for the purification of R-phycoerythrin (R-PE) of Grateloupia turuturu Yamada is described. Native R-PE was obtained with a purity index of 2.89 and a recovery yield of 27% using DEAE-Sepharose Fast Flow chromatography with a three-step increase in ionic strength. The analysis by SDS electrophoresis showed a broad band between 18 and 21kDa in size corresponding to subunits α and β and a low intensity band of 29kDa corresponding to the γ subunit. Two forms of R-PE were identified by gel filtration chromatography: a native form with a molecular weight of 260±5kDa and a dissociated form with a molecular weight of 60±2kDa. The native form presented the characteristic absorption spectrum of R-PE with three absorbance maxima at 498, 540 and 565nm, whereas the dissociated form presented only the 498 and 540nm peaks. Moreover, the two forms displayed two different fluorescence maxima.
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seasonal variation in the antivibrio activity of two organic extracts from two red seaweed palmaria palmata and the introduced Grateloupia turuturu against the abalone pathogen vibrio harveyi
Aquatic Living Resources, 2015Co-Authors: Nuria Garciabueno, Thomas Guerin, Yves Francois Pouchus, Christine Paillard, Vincent Turpin, Justine Dumay, Valerie Stigerpouvreau, Arnaldo Aitor MarinatuchaAbstract:The wide polarity range and highly polar compounds of two selected red seaweed, Grateloupia turuturu and Palmaria palmata were extracted using two different types of solvent, dichloromethane/methanol and methanol/water. Monthly in vitro antibacterial activities were studied using the microplate method against the marine bacteria Vibrio harveyi strain ORM4, known to infect abalone. Inhibition, slowdown and delay of Vibrio harveyi growth were investigated. Polar compounds of seaweed showed an activity against the abalone pathogen. The best activity was recorded from P. palmata collected in spring, with an inhibition of 7.9% of the bacterial growth. Preliminary 1H NMR profiles identified the differences between the extracts.
Michele Morancais - One of the best experts on this subject based on the ideXlab platform.
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can the european abalone haliotis tuberculata survive on an invasive algae a comparison of the nutritional value of the introduced Grateloupia turuturu and the native palmaria palmata for the commercial european abalone industry
Journal of Applied Phycology, 2016Co-Authors: Nuria Garciabueno, Vincent Turpin, Justine Dumay, Michele Morancais, Bruno Cognie, Mireille Amat, Jeanmarie Pedron, Arnaldo Marin Atucha, Joel FleurenceAbstract:The suitability of two red algae species, the introduced Grateloupia turuturu and the native Palmaria palmata, as feed for the culture of the European abalone Haliotis tuberculata, was compared over a 5-month period. Three experimental diets were tested: (1) P. palmata, (2) G. turuturu, and (3) a mixed diet of P. palmata and G. turuturu (1:1). Biochemical composition (proteins, carbohydrates, lipids, ashes) of algae was measured. No mortality was observed during the time of the experiment in any of the treatments. Growth in length and weight was higher for abalone fed with P. palmata and the mixed diet. Abalone exhibited a preference for P. palmata and showed the highest weight gain with this macroalgae (107.8 ± 7.2 %). Grateloupia turuturu disintegrated faster in abalone rearing conditions and was not suitable for significant growth of H. tuberculata in a monospecific diet. However, when it is provided in a mixed diet, abalone seem to show a higher lipid content than when fed a P. palmata monospecific diet. The invasive G. turuturu could be an interesting food supplement for European abalone.
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one step purification of r phycoerythrin from the red edible seaweed Grateloupia turuturu
Journal of Chromatography B, 2015Co-Authors: Mathilde Munier, Justine Dumay, Michele Morancais, Pascal Jaouen, Joel FleurenceAbstract:A one-step chromatographic method for the purification of R-phycoerythrin (R-PE) of Grateloupia turuturu Yamada is described. Native R-PE was obtained with a purity index of 2.89 and a recovery yield of 27% using DEAE-Sepharose Fast Flow chromatography with a three-step increase in ionic strength. The analysis by SDS electrophoresis showed a broad band between 18 and 21kDa in size corresponding to subunits α and β and a low intensity band of 29kDa corresponding to the γ subunit. Two forms of R-PE were identified by gel filtration chromatography: a native form with a molecular weight of 260±5kDa and a dissociated form with a molecular weight of 60±2kDa. The native form presented the characteristic absorption spectrum of R-PE with three absorbance maxima at 498, 540 and 565nm, whereas the dissociated form presented only the 498 and 540nm peaks. Moreover, the two forms displayed two different fluorescence maxima.
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physicochemical factors affecting the stability of two pigments r phycoerythrin of Grateloupia turuturu and b phycoerythrin of porphyridium cruentum
Food Chemistry, 2014Co-Authors: Mathilde Munier, Justine Dumay, Michele Morancais, Pascal Jaouen, Alva Wijaya, Joel FleurenceAbstract:Abstract Phycoerythrin is a major light-harvesting pigment of red algae, which could be used as a natural dye in foods. The stability of R-phycoerythrin of Grateloupia turuturu and B-phycoerythrin of Porphyridium cruentum in relation to different light exposure times, pHs, and temperatures was studied. Regarding the light exposure time, after 48 h, the reduction in concentrations of B-phycoerythrin and R-phycoerythrin were 30 ± 2.4% and 70 ± 1%, respectively. Phycoerythrins presented good stability from pH 4 to 10. At pH 2, the reduction in concentration was 90 ± 4% for B-phycoerythrin and 40 ± 2.5% for R-phycoerythrin while, at pH 12, the phycoerythrins were degraded. Phycoerythrins showed good stability toward temperature, up to 40 °C. At 60 °C, the reduction in concentrations of B-phycoerythrin and R-phycoerythrin were 50 ± 3.4% and 70 ± 0.18%, respectively. Moreover, the best conditions of storage (−20 °C) were determined.
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variation in the biochemical composition of the edible seaweed Grateloupia turuturu yamada harvested from two sampling sites on the brittany coast france the influence of storage method on the extraction of the seaweed pigment r phycoerythrin
Journal of Chemistry, 2013Co-Authors: Mathilde Munier, Justine Dumay, Michele Morancais, Pascal Jaouen, Joel FleurenceAbstract:Numerous studies have demonstrated that the biochemical content of seaweeds varies according to seasonality in a restricted area. In this study, the influence of sampling site on the biochemical composition of the edible red seaweed Grateloupia turuturu Yamada was investigated, but not its variation over time. Some differences in water-soluble protein ( m dw and m dw), water-soluble carbohydrate ( m dw and m dw), and lipid contents ( m dw and m dw) were recorded between the two sites chosen on the Brittany coast (France). The yield of R-phycoerythrin (R-PE) contained in the seaweed also varied according to the sampling site ( m dw versus m dw). In addition, the effect of storage conditions on the preservation of R-PE was studied. The results demonstrated that freezing is the best preservation method in terms of R-PE extraction yield and purity index. In conclusion, this study shows that the sampling site influences the biochemical content of the red seaweed Grateloupia turuturu. Moreover, the extraction yield of R-phycoerythrin and its purity index depend on both the sampling site and the sample storage method.
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study of the chemical composition of edible red macroalgae Grateloupia turuturu from brittany france
Food Chemistry, 2010Co-Authors: Claire Denis, Michele Morancais, Pascal Jaouen, Pierre Gaudin, Estelle Deniaud, Gaetane Wielgoszcollin, Gilles Barnathan, Joel FleurenceAbstract:The chemical composition and its seasonal variation of the red seaweed Grateloupia turuturu, an invasive macroalgae from Brittany, France, were investigated. Size, ash, protein, lipid, dietary fibre (soluble, insoluble and total), protein pigment (R-phycoerythrin, R-phycocyanin), and fatty acid content were measured in G. turuturu samples collected over 1 year (2006). The average size of this seaweed was 32.0 cm long and approximately 5.0 cm wide, while the size of the thallus was maximal in June (in length and width). On the dry weight basis, this alga was constituted of more than 18% ash, about 23% protein, 2.6% lipids, and approximately 60% dietary fibre. The most abundant fatty acids were palmitic acid and eicosapentaenoic acid (52% and 12% of the fatty acid fraction, respectively). The study of seasonal variations showed that the best period to collect the seaweed for food use is between February and June.
Vincent Turpin - One of the best experts on this subject based on the ideXlab platform.
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can the european abalone haliotis tuberculata survive on an invasive algae a comparison of the nutritional value of the introduced Grateloupia turuturu and the native palmaria palmata for the commercial european abalone industry
Journal of Applied Phycology, 2016Co-Authors: Nuria Garciabueno, Vincent Turpin, Justine Dumay, Michele Morancais, Bruno Cognie, Mireille Amat, Jeanmarie Pedron, Arnaldo Marin Atucha, Joel FleurenceAbstract:The suitability of two red algae species, the introduced Grateloupia turuturu and the native Palmaria palmata, as feed for the culture of the European abalone Haliotis tuberculata, was compared over a 5-month period. Three experimental diets were tested: (1) P. palmata, (2) G. turuturu, and (3) a mixed diet of P. palmata and G. turuturu (1:1). Biochemical composition (proteins, carbohydrates, lipids, ashes) of algae was measured. No mortality was observed during the time of the experiment in any of the treatments. Growth in length and weight was higher for abalone fed with P. palmata and the mixed diet. Abalone exhibited a preference for P. palmata and showed the highest weight gain with this macroalgae (107.8 ± 7.2 %). Grateloupia turuturu disintegrated faster in abalone rearing conditions and was not suitable for significant growth of H. tuberculata in a monospecific diet. However, when it is provided in a mixed diet, abalone seem to show a higher lipid content than when fed a P. palmata monospecific diet. The invasive G. turuturu could be an interesting food supplement for European abalone.
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Seasonal variation in the antivibrio activity of two organic extracts from two red seaweed:
'EDP Sciences', 2016Co-Authors: Nuria García-bueno, Thomas Guerin, Yves Francois Pouchus, Christine Paillard, Vincent Turpin, Priscilla Decottignies, Justine Dumay, Valérie Stiger-pouvreau, Arnaldo Aitor Marín-atucha, Joel FleurenceAbstract:The wide polarity range and highly polar compounds of two selected red seaweed, Grateloupia turuturu and Palmaria palmata were extracted using two different types of solvent, dichloromethane/methanol and methanol/water. Monthly in vitro antibacterial activities were studied using the microplate method against the marine bacteria Vibrio harveyi strain ORM4, known to infect abalone. Inhibition, slowdown and delay of Vibrio harveyi growth were investigated. Polar compounds of seaweed showed an activity against the abalone pathogen. The best activity was recorded from P. palmata collected in spring, with an inhibition of 7.9% of the bacterial growth. Preliminary 1H NMR profiles identified the differences between the extracts
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seasonal variation in the antivibrio activity of two organic extracts from two red seaweed palmaria palmata and the introduced Grateloupia turuturu against the abalone pathogen vibrio harveyi
Aquatic Living Resources, 2015Co-Authors: Nuria Garciabueno, Thomas Guerin, Yves Francois Pouchus, Christine Paillard, Vincent Turpin, Justine Dumay, Valerie Stigerpouvreau, Arnaldo Aitor MarinatuchaAbstract:The wide polarity range and highly polar compounds of two selected red seaweed, Grateloupia turuturu and Palmaria palmata were extracted using two different types of solvent, dichloromethane/methanol and methanol/water. Monthly in vitro antibacterial activities were studied using the microplate method against the marine bacteria Vibrio harveyi strain ORM4, known to infect abalone. Inhibition, slowdown and delay of Vibrio harveyi growth were investigated. Polar compounds of seaweed showed an activity against the abalone pathogen. The best activity was recorded from P. palmata collected in spring, with an inhibition of 7.9% of the bacterial growth. Preliminary 1H NMR profiles identified the differences between the extracts.
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seasonal antibacterial activity of two red seaweeds palmaria palmata and Grateloupia turuturu on european abalone pathogen vibrio harveyi
Aquatic Living Resources, 2014Co-Authors: Nuria Garciabueno, Yves Francois Pouchus, Christine Paillard, Vincent Turpin, Priscilla Decottignies, Justine Dumay, Valerie Stigerpouvreau, Nelly Kervarec, Arnaldo Aitor MarinatuchaAbstract:Vibrio harveyi is the main pathogen of the European abalone Haliotis tuberculata, and recently caused important mortalities at the production sites of this marine gastropod in France. In the present work, the monthly an- tibacterial activity of two red seaweed species from the French Atlantic coast, the native Palmaria palmata and the intro- duced Grateloupia turuturu, were investigated against the abalone pathogen Vibrio harveyi strain ORM4. Water-soluble extracts were screened using the microplate method. Grateloupia turuturu showed an antibacterial activity with a max- imal growth inhibition in spring of around 16%. In contrast, Palmaria palmata was inactive, as further growth of the bacteria was observed. Preliminary one-dimensional proton nuclear magnetic-resonance ( 1 H NMR) profiles identified the differences between the two water-soluble extracts.
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seasonal antibacterial activity of two red seaweeds palmaria palmata and Grateloupia turuturu on european abalone pathogen vibrio harveyi
Aquatic Living Resources, 2014Co-Authors: Nuria Garciabueno, Yves Francois Pouchus, Christine Paillard, Vincent Turpin, Priscilla Decottignies, Justine Dumay, Valerie Stigerpouvreau, Nelly Kervarec, Arnaldo Aitor MarinatuchaAbstract:Vibrio harveyi is the main pathogen of the European abalone Haliotis tuberculata, and recently caused important mortalities at the production sites of this marine gastropod in France. In the present work, the monthly an- tibacterial activity of two red seaweed species from the French Atlantic coast, the native Palmaria palmata and the intro- duced Grateloupia turuturu, were investigated against the abalone pathogen Vibrio harveyi strain ORM4. Water-soluble extracts were screened using the microplate method. Grateloupia turuturu showed an antibacterial activity with a max- imal growth inhibition in spring of around 16%. In contrast, Palmaria palmata was inactive, as further growth of the bacteria was observed. Preliminary one-dimensional proton nuclear magnetic-resonance ( 1 H NMR) profiles identified the differences between the two water-soluble extracts.
