Haematoxylin

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Li Deng - One of the best experts on this subject based on the ideXlab platform.

  • modification of resin semithin section staining method in plant tissues
    Plant Physiology, 2011
    Co-Authors: Li Deng
    Abstract:

    In this study, a new resin semithin section staining method suitable to plant tissue-section was established. Young roots, young stems, leaves and ovules of cotton (Gossypium hirsutum) and stems of maize (Zea mays) were analyzed by the methods of resin semithin section. The improved staining methods (including safranin-fast green staining, hematoxylin-eosin staining, FeCl3-Haematoxylin staining) were applied in the preparation of the plant semithin section. Our experimental results indicated that clear-staining and integral-structural semithin section of cotton and maize tissues were obtained by using modified methods of resin semithin section.

Mehdi Bayat - One of the best experts on this subject based on the ideXlab platform.

  • Electrochemical oxidation of hematoxylin – Part 1: Experimental and theoretical studies in an aqueous acidic medium
    Journal of Electroanalytical Chemistry, 2012
    Co-Authors: Hadi Beiginejad, Davood Nematollahi, Mehdi Bayat
    Abstract:

    Abstract The electrochemical oxidation of hematoxylin in acidic solutions has been studied using cyclic voltammetry, differential pulse voltammetry, controlled potential coulometry and digital simulation methods. The results revealed that hematoxylin shows two oxidation–reduction peaks ( EE mechanism). In the first step hematoxylin is converted to hematein via a two-electron process. Five possible structures were designed for the first oxidation step and optimized at the B3LYP/6-311 + G ∗∗ level of theory. Also, the electrochemical oxidation of hematoxylin has been studied in the presence of p -toluenesulfinic acid as a nucleophile. The results showed that the electrochemically generated hematein participates in Michael addition reaction with p -toluenesulfinic acid via a novel “ E-Tautomerization - C ” mechanism and is converted to an unexpected isomer of tolylsulfonylhematoxylin. In this work, an electrochemical method for the synthesis of a new hematoxylin derivative is also reported.

  • electrochemical oxidation of hematoxylin part 1 experimental and theoretical studies in an aqueous acidic medium
    Journal of Electroanalytical Chemistry, 2012
    Co-Authors: Hadi Beiginejad, Davood Nematollahi, Mehdi Bayat
    Abstract:

    Abstract The electrochemical oxidation of hematoxylin in acidic solutions has been studied using cyclic voltammetry, differential pulse voltammetry, controlled potential coulometry and digital simulation methods. The results revealed that hematoxylin shows two oxidation–reduction peaks ( EE mechanism). In the first step hematoxylin is converted to hematein via a two-electron process. Five possible structures were designed for the first oxidation step and optimized at the B3LYP/6-311 + G ∗∗ level of theory. Also, the electrochemical oxidation of hematoxylin has been studied in the presence of p -toluenesulfinic acid as a nucleophile. The results showed that the electrochemically generated hematein participates in Michael addition reaction with p -toluenesulfinic acid via a novel “ E-Tautomerization - C ” mechanism and is converted to an unexpected isomer of tolylsulfonylhematoxylin. In this work, an electrochemical method for the synthesis of a new hematoxylin derivative is also reported.

Hadi Beiginejad - One of the best experts on this subject based on the ideXlab platform.

  • Electrochemical oxidation and adsorption of hematoxylin at glassy carbon electrode in various pH values
    Journal of The Iranian Chemical Society, 2014
    Co-Authors: Hadi Beiginejad, Davood Nematollahi, Mohammad Noroozi, Shahram Lotfi
    Abstract:

    Electrochemical oxidation of hematoxylin has been studied using cyclic voltammetry, differential pulse voltammetry, chronocoulometry and controlled potential coulometry. It indicates that not only electrochemical behavior of hematoxylin depends on the pH, but also its behavior depends on the concentration. The adsorption activity of hematoxylin and its oxidation products on the surface of the glassy carbon electrode was found to vary in the order hematox > hematein > hematoxylin. In addition, it is found that adsorption of hematoxylin depends on the pH and increases with decreasing pH. It is also found that at pHs lower than pKa1, the adsorption of hematoxylin on the surface of the glassy carbon electrode is high. Furthermore, the orientation of adsorbed hematoxylin on the surface of glassy carbon electrode was estimated. The results show that the orientation of adsorbed hematoxylin molecules at glassy carbon surface is endwise.

  • Electrochemical oxidation of hematoxylin – Part 1: Experimental and theoretical studies in an aqueous acidic medium
    Journal of Electroanalytical Chemistry, 2012
    Co-Authors: Hadi Beiginejad, Davood Nematollahi, Mehdi Bayat
    Abstract:

    Abstract The electrochemical oxidation of hematoxylin in acidic solutions has been studied using cyclic voltammetry, differential pulse voltammetry, controlled potential coulometry and digital simulation methods. The results revealed that hematoxylin shows two oxidation–reduction peaks ( EE mechanism). In the first step hematoxylin is converted to hematein via a two-electron process. Five possible structures were designed for the first oxidation step and optimized at the B3LYP/6-311 + G ∗∗ level of theory. Also, the electrochemical oxidation of hematoxylin has been studied in the presence of p -toluenesulfinic acid as a nucleophile. The results showed that the electrochemically generated hematein participates in Michael addition reaction with p -toluenesulfinic acid via a novel “ E-Tautomerization - C ” mechanism and is converted to an unexpected isomer of tolylsulfonylhematoxylin. In this work, an electrochemical method for the synthesis of a new hematoxylin derivative is also reported.

  • electrochemical oxidation of hematoxylin part 1 experimental and theoretical studies in an aqueous acidic medium
    Journal of Electroanalytical Chemistry, 2012
    Co-Authors: Hadi Beiginejad, Davood Nematollahi, Mehdi Bayat
    Abstract:

    Abstract The electrochemical oxidation of hematoxylin in acidic solutions has been studied using cyclic voltammetry, differential pulse voltammetry, controlled potential coulometry and digital simulation methods. The results revealed that hematoxylin shows two oxidation–reduction peaks ( EE mechanism). In the first step hematoxylin is converted to hematein via a two-electron process. Five possible structures were designed for the first oxidation step and optimized at the B3LYP/6-311 + G ∗∗ level of theory. Also, the electrochemical oxidation of hematoxylin has been studied in the presence of p -toluenesulfinic acid as a nucleophile. The results showed that the electrochemically generated hematein participates in Michael addition reaction with p -toluenesulfinic acid via a novel “ E-Tautomerization - C ” mechanism and is converted to an unexpected isomer of tolylsulfonylhematoxylin. In this work, an electrochemical method for the synthesis of a new hematoxylin derivative is also reported.

Lucio Merlini - One of the best experts on this subject based on the ideXlab platform.

  • Synthesis and sweet taste of optically active (–)-Haematoxylin and of some (±)-Haematoxylin derivatives
    J. Chem. Soc. Perkin Trans. 1, 1995
    Co-Authors: Anna Arnoldi, Angela Bassoli, Gigliola Borgonovo, Lucio Merlini
    Abstract:

    In order to explain the sweet taste of the natural polyphenolic compound (+)-Haematoxylin 1, four (±)-Haematoxylin derivatives 4–7 and the enantiomer (–)-Haematoxylin have been synthesized and tasted. Unlike Haematoxylin, the derivatives 4–7 have a restricted number of different possibilities of binding to the sweet taste receptor according to the Shallenberger–Acree–Kier model. This allowed the study of the most likely orientation of these compounds in the active site of receptor. The results are supported by the comparison of the molecular structures with the receptor models of Temussi–Goodman and Tinti–Nofre. The synthesis of the (–)-enantiomer of Haematoxylin also allows for discussion of the relationship between configuration and sweet taste in these compounds.

  • synthesis and sweet taste of optically active Haematoxylin and of some Haematoxylin derivatives
    Journal of The Chemical Society-perkin Transactions 1, 1995
    Co-Authors: Anna Arnoldi, Angela Bassoli, Gigliola Borgonovo, Lucio Merlini
    Abstract:

    In order to explain the sweet taste of the natural polyphenolic compound (+)-Haematoxylin 1, four (±)-Haematoxylin derivatives 4–7 and the enantiomer (–)-Haematoxylin have been synthesized and tasted. Unlike Haematoxylin, the derivatives 4–7 have a restricted number of different possibilities of binding to the sweet taste receptor according to the Shallenberger–Acree–Kier model. This allowed the study of the most likely orientation of these compounds in the active site of receptor. The results are supported by the comparison of the molecular structures with the receptor models of Temussi–Goodman and Tinti–Nofre. The synthesis of the (–)-enantiomer of Haematoxylin also allows for discussion of the relationship between configuration and sweet taste in these compounds.

Daniel A. Davis - One of the best experts on this subject based on the ideXlab platform.

  • Optimize Oxidation for the Fastest Hematoxylin Staining
    Dermatologic Surgery, 2012
    Co-Authors: Daniel A. Davis
    Abstract:

    Background Hematoxylin solutions are complex and require additives to produce purple-blue colorfast staining. Objective To determine the best hematoxylin cocktail and fastest immersion times for frozen tissue preparation. Materials and Methods Hematoxylin solution type, oxidizer concentration, and immersion time were tested simultaneously on normal skin frozen sections. Results Regressive hematoxylin solutions stained faster than progressive solutions. Permanganate oxidized premixed hematoxylin best. Conclusion Augmenting premixed regressive hematoxylin solution with permanganate provides rapid staining, which is advantageous for frozen sections.