The Experts below are selected from a list of 2829 Experts worldwide ranked by ideXlab platform
Jia Aiqing - One of the best experts on this subject based on the ideXlab platform.
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Cloning and of structure prediction of outer membrane protein P5 gene of Haemophilus parasuis.
Chinese journal of veterinary science, 2010Co-Authors: Chen Shanzhen, Jia Aiqing, Li Chunling, Wang Guiping, Zeng Zhenling, Yang DongxiaAbstract:A pair of primers were designed according to Haemophilus parasuis outer membrane protein gene sequences published in the GenBank,and outer membrane protein gene 5(OMP5) of Haemophilus parasuis isolate was amplified by PCR.The fragment was inserted into the pMD18-T vector,then sequenced and the outer membrane protein secondary structure,antigenic determinants,signal peptide and transmembrane domain of Haemophilus parasuis were predicted with the bioinformatics and molecular biology software.The result showed the OMP5 of HPS GDH15 was 1 116 bp in size,encoding 371 amino acid residues.Compared with the SH0165 outer membrane protein gene published in GenBank,the homology of nucleotide sequence and amino acid were both 100%.The protein structure prediction showed that it was a mixed pattern protein,containing the α-helix,β-pleated sheet,β-turn and random coil.Regions of β-turn and random coil would become the antigenic epitopes.It has 15 antigenic determinants,1 signal peptide and the best cutting site was between amino acids 21 and 22.But transmembrane domain did not exist in it and no similar three dimensional structure was found.The OMP5 of Haemophilus parasuis GDH15 isolate was conservative,it could be used for the development of genetic engineering vaccine and ELISA method.
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Establishment and application of dulex PCR assay for identification of Haemophilus parasuis and Streptococcus suis.
Chinese journal of veterinary science, 2009Co-Authors: Jia Aiqing, Li Chunling, Wang Guiping, Yang Dongxia, He Lili, Wang JinshengAbstract:Haemophilus parasuis and Streptococcus suis were important pathogenes of swine both causing meningitis,arthritis,endocarditis,and it was difficult to distinguish them from the clinical symptom.In this study,the sequence of Haemophilus parasuis and Streptococcus suis 16S rRNA gene were amplified by polymerase chain reaction(PCR),and 822 bp and 294 bp specific DNA fragment could be amplified.Dulex PCR with the specific primer for differentiating Haemophilus parasuis and Streptococcus suis in pigs was established,and it was conformed to be specificity and sensitivity by clinical application.This method was applied in 161 diseased pig herds Haemophilus parasuis was 23.75% positive,and Streptococcus suis was 43.48% positive.
Wang Jinsheng - One of the best experts on this subject based on the ideXlab platform.
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Establishment and application of dulex PCR assay for identification of Haemophilus parasuis and Streptococcus suis.
Chinese journal of veterinary science, 2009Co-Authors: Jia Aiqing, Li Chunling, Wang Guiping, Yang Dongxia, He Lili, Wang JinshengAbstract:Haemophilus parasuis and Streptococcus suis were important pathogenes of swine both causing meningitis,arthritis,endocarditis,and it was difficult to distinguish them from the clinical symptom.In this study,the sequence of Haemophilus parasuis and Streptococcus suis 16S rRNA gene were amplified by polymerase chain reaction(PCR),and 822 bp and 294 bp specific DNA fragment could be amplified.Dulex PCR with the specific primer for differentiating Haemophilus parasuis and Streptococcus suis in pigs was established,and it was conformed to be specificity and sensitivity by clinical application.This method was applied in 161 diseased pig herds Haemophilus parasuis was 23.75% positive,and Streptococcus suis was 43.48% positive.
Yang Dongxia - One of the best experts on this subject based on the ideXlab platform.
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Cloning and of structure prediction of outer membrane protein P5 gene of Haemophilus parasuis.
Chinese journal of veterinary science, 2010Co-Authors: Chen Shanzhen, Jia Aiqing, Li Chunling, Wang Guiping, Zeng Zhenling, Yang DongxiaAbstract:A pair of primers were designed according to Haemophilus parasuis outer membrane protein gene sequences published in the GenBank,and outer membrane protein gene 5(OMP5) of Haemophilus parasuis isolate was amplified by PCR.The fragment was inserted into the pMD18-T vector,then sequenced and the outer membrane protein secondary structure,antigenic determinants,signal peptide and transmembrane domain of Haemophilus parasuis were predicted with the bioinformatics and molecular biology software.The result showed the OMP5 of HPS GDH15 was 1 116 bp in size,encoding 371 amino acid residues.Compared with the SH0165 outer membrane protein gene published in GenBank,the homology of nucleotide sequence and amino acid were both 100%.The protein structure prediction showed that it was a mixed pattern protein,containing the α-helix,β-pleated sheet,β-turn and random coil.Regions of β-turn and random coil would become the antigenic epitopes.It has 15 antigenic determinants,1 signal peptide and the best cutting site was between amino acids 21 and 22.But transmembrane domain did not exist in it and no similar three dimensional structure was found.The OMP5 of Haemophilus parasuis GDH15 isolate was conservative,it could be used for the development of genetic engineering vaccine and ELISA method.
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Establishment and application of dulex PCR assay for identification of Haemophilus parasuis and Streptococcus suis.
Chinese journal of veterinary science, 2009Co-Authors: Jia Aiqing, Li Chunling, Wang Guiping, Yang Dongxia, He Lili, Wang JinshengAbstract:Haemophilus parasuis and Streptococcus suis were important pathogenes of swine both causing meningitis,arthritis,endocarditis,and it was difficult to distinguish them from the clinical symptom.In this study,the sequence of Haemophilus parasuis and Streptococcus suis 16S rRNA gene were amplified by polymerase chain reaction(PCR),and 822 bp and 294 bp specific DNA fragment could be amplified.Dulex PCR with the specific primer for differentiating Haemophilus parasuis and Streptococcus suis in pigs was established,and it was conformed to be specificity and sensitivity by clinical application.This method was applied in 161 diseased pig herds Haemophilus parasuis was 23.75% positive,and Streptococcus suis was 43.48% positive.
Li L - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial activity in vitro of 16 kinds of traditional Chinese medicine extracts against Haemophilus parasuis
Chinese journal of veterinary science, 2014Co-Authors: Li LAbstract:To get traditional Chinese medicine which could control Haemophilus parasuis effectively,the antimicrobial activitiy in vitro of extracts from 16 kinds of traditional Chinese medicine against Haemophilus parasuis were investigated with methods of serial two-fold broth dilution method and agar plate counting method.The results showed that RizomaCoptidisand Cortex Phellodendr Chinensis had the best antimicrobial activity agianst Haemophilus parasuis,the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)was 0.98g/L.Meanwhile,MIC of Radix Scutellariae,Rhizoma Polygoni Cuspidati,Cortex Fraxini was 1.95g/L,the antibacterial effect was better.Other ten kinds of traditional Chinese medicine extracts such as Herba Taraxaci,Fructus Forsythiae and so on,their MIC range was 3.90-62.50g/L.The antibacterial effect of Cortex Dictamni and Herba Violae was poorer,their MIC was 125.00g/L.
Wang Guiping - One of the best experts on this subject based on the ideXlab platform.
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Cloning and of structure prediction of outer membrane protein P5 gene of Haemophilus parasuis.
Chinese journal of veterinary science, 2010Co-Authors: Chen Shanzhen, Jia Aiqing, Li Chunling, Wang Guiping, Zeng Zhenling, Yang DongxiaAbstract:A pair of primers were designed according to Haemophilus parasuis outer membrane protein gene sequences published in the GenBank,and outer membrane protein gene 5(OMP5) of Haemophilus parasuis isolate was amplified by PCR.The fragment was inserted into the pMD18-T vector,then sequenced and the outer membrane protein secondary structure,antigenic determinants,signal peptide and transmembrane domain of Haemophilus parasuis were predicted with the bioinformatics and molecular biology software.The result showed the OMP5 of HPS GDH15 was 1 116 bp in size,encoding 371 amino acid residues.Compared with the SH0165 outer membrane protein gene published in GenBank,the homology of nucleotide sequence and amino acid were both 100%.The protein structure prediction showed that it was a mixed pattern protein,containing the α-helix,β-pleated sheet,β-turn and random coil.Regions of β-turn and random coil would become the antigenic epitopes.It has 15 antigenic determinants,1 signal peptide and the best cutting site was between amino acids 21 and 22.But transmembrane domain did not exist in it and no similar three dimensional structure was found.The OMP5 of Haemophilus parasuis GDH15 isolate was conservative,it could be used for the development of genetic engineering vaccine and ELISA method.
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Establishment and application of dulex PCR assay for identification of Haemophilus parasuis and Streptococcus suis.
Chinese journal of veterinary science, 2009Co-Authors: Jia Aiqing, Li Chunling, Wang Guiping, Yang Dongxia, He Lili, Wang JinshengAbstract:Haemophilus parasuis and Streptococcus suis were important pathogenes of swine both causing meningitis,arthritis,endocarditis,and it was difficult to distinguish them from the clinical symptom.In this study,the sequence of Haemophilus parasuis and Streptococcus suis 16S rRNA gene were amplified by polymerase chain reaction(PCR),and 822 bp and 294 bp specific DNA fragment could be amplified.Dulex PCR with the specific primer for differentiating Haemophilus parasuis and Streptococcus suis in pigs was established,and it was conformed to be specificity and sensitivity by clinical application.This method was applied in 161 diseased pig herds Haemophilus parasuis was 23.75% positive,and Streptococcus suis was 43.48% positive.
