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Da Yong Zhou - One of the best experts on this subject based on the ideXlab platform.
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evaluation of lipid profile in different tissues of japanese abalone Haliotis Discus Hannai ino with uplc esi q tof ms based lipidomic study
Food Chemistry, 2018Co-Authors: Yuying Zhang, Bei Wei Zhu, Da Yong Zhou, Lei Qin, Yuxi Liu, Megan ThorntonAbstract:Abalone has been farmed commercially for the last few decades, and the breeding and economic value of abalone have gained extensive attention. In this study, the lipid profile of the foot muscles, viscera, and gonads of male and female Japanese abalone Haliotis Discus Hannai Ino was explored using ultra performance liquid chromatography-electrospray ionization-quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS), to Discuss the effect of lipid composition on its nutritional value. Thirty-four species from ten lipid classes including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerol, phosphatidic acid, lysophosphatidic acid, triacylglycerol, steroids, terpenoids and fatty acids were annotated by MS-DIAL to obtain all fragment ions for precursors. Glycerophospholipids (GPLs) enriched in unsaturated fatty acids were the major components, which accounted for 52-57% of total lipids. Considering the high-level of GPLs, and their importance in maintaining the integrity and functionality of cell membranes, further utilization of inexpensive tissues, such as viscera and gonads is warranted.
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anticoagulant activity and structural characterization of polysaccharide from abalone Haliotis Discus Hannai ino gonad
Molecules, 2016Co-Authors: Jun Zhao, Jing Feng Yang, Da Yong Zhou, Shuang Song, Weizhou Qiao, Ce Zhu, Shuyin Liu, Bei Wei ZhuAbstract:In this study, we aimed at characterizing the structure and the anticoagulant activity of a polysaccharide fraction (AGP33) isolated from the gonads of Haliotis Discus Hannai Ino. AGP33 was extracted by enzymatic hydrolysis and purified by ion-exchange and gel-filtration chromatography. The backbone fraction of AGP33 (BAGP33), which appeared to contain of mannose, glucose and galactose, was prepared by partial acid hydrolysis. According to methylation and nuclear magnetic resonance (NMR) spectroscopy, the backbone of AGP33 was identified as mainly consisting of 1→3-linked, 1→4-linked, and 1→6-linked monosaccharides. AGP33 is a sulfated polysaccharide with sulfates occur at 3-O- and 4-O-positions. It prolonged thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) compared to a saline control solution in a dosage-dependent manner. AGP33 exhibited an extension (p < 0.01) of APTT compared to the saline group at concentrations higher than 5 μg/mL. AGP33 exhibited higher anticoagulant activity than its desulfated product (AGP33-des) and BAGP33. The results showed that polysaccharide with higher molecular weight and sulfate content demonstrated greater anticoagulant activity.
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effects of heating conditions on fatty acids and volatile compounds in foot muscle of abalone Haliotis Discus Hannai ino
Fisheries Science, 2014Co-Authors: Hongxu Wang, Da Yong Zhou, Lei Qin, Yan Wang, Shuang Song, Xusong Wang, Bei Wei ZhuAbstract:Abalone Haliotis Discus Hannai Ino is a popular delicacy consumed as a luxury food owing to its unique flavor and texture. In this study, we investigated the effects of heating conditions on the fatty acids and volatile compounds in its foot muscle based on gas chromatography–mass spectrometry (GC–MS) and solid-phase microextraction (SPME)–GC–MS. The contents of fatty acids significantly decreased after heating at 80 °C for 2 h. In total, 52 volatile compounds, including aldehydes, aromatic compounds, alkanes, alcohols, ketones, and furans, were detected in the heated samples. Principal component analysis revealed an interaction between heating temperature and time. Heating at 80 °C for 0.5–2 h generated higher contents of volatile compounds. In particular, the contents of hexanal, heptanal, octanal, nonanal, and undecanal mainly derived from autoxidation of fatty acids during heating increased at least fourfold.
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antioxidant activity of hydrolysates obtained from scallop patinopecten yessoensis and abalone Haliotis Discus Hannai ino muscle
Food Chemistry, 2012Co-Authors: Da Yong Zhou, Jing Feng Yang, Dong Mei Li, Yue Tang, Yoshiyuki MurataAbstract:Scallop (Patinopecten yessoensis) and abalone (Haliotis Discus Hannai Ino) muscle were hydrolysed with commercially available food-grade proteases. The resulting hydrolysates showed DPPH and hydroxyl radicals scavenging abilities, reducing power, and ferrous ion chelating capacity. The antioxidant activities of hydrolysate of abalone foot muscle (HAFM) increased with increasing incubation time during the whole hydrolysis process in 180 min. Whereas, the antioxidant activities of hydrolysate of scallop adductor muscle (HSAM) increased at initial stage and peaked after 25–30 min of hydrolysis, and then gradually decreased thereafter. Compared with HAFM, HSAM with comparable hydrolysis time contained more free amino acids (FAA) and small-sized peptides (below 500 Da), which may account for the differences in antioxidant activities versus hydrolysis time curves of the two hydrolysates. The above results indicate that limited hydrolysis of proteins can increase their antioxidant activity, whereas extensive hydrolysis can decrease it.
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in vitro antioxidant activity of enzymatic hydrolysates prepared from abalone Haliotis Discus Hannai ino viscera
Food and Bioproducts Processing, 2012Co-Authors: Da Yong Zhou, Bei Wei Zhu, Jing Feng Yang, Hai Tao Wu, Dong Mei Li, Lu Qiao, Yoshiyuki MurataAbstract:Pacific abalone (Haliotis Discus Hannai Ino) viscera, which are normally discarded as byproducts, were hydrolyzed with five commercially available proteases, including alkali protease, papain, neutral protease, pepsin, and trypsin. The hydrolysates obtained were fractionated using ultrafiltration membrane bioreactor system. The resulting hydrolysate fractions were investigated for their antioxidant activities, including the scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, the scavenging effect on hydroxyl radical, and the reducing power. Results showed that the hydrolysate fractions were effective antioxidants, and the antioxidant activity of the hydrolysates was related to the enzyme type employed. As byproducts, abalone viscera can be used as a potential source of antioxidant peptides.
Bei Wei Zhu - One of the best experts on this subject based on the ideXlab platform.
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evaluation of lipid profile in different tissues of japanese abalone Haliotis Discus Hannai ino with uplc esi q tof ms based lipidomic study
Food Chemistry, 2018Co-Authors: Yuying Zhang, Bei Wei Zhu, Da Yong Zhou, Lei Qin, Yuxi Liu, Megan ThorntonAbstract:Abalone has been farmed commercially for the last few decades, and the breeding and economic value of abalone have gained extensive attention. In this study, the lipid profile of the foot muscles, viscera, and gonads of male and female Japanese abalone Haliotis Discus Hannai Ino was explored using ultra performance liquid chromatography-electrospray ionization-quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS), to Discuss the effect of lipid composition on its nutritional value. Thirty-four species from ten lipid classes including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerol, phosphatidic acid, lysophosphatidic acid, triacylglycerol, steroids, terpenoids and fatty acids were annotated by MS-DIAL to obtain all fragment ions for precursors. Glycerophospholipids (GPLs) enriched in unsaturated fatty acids were the major components, which accounted for 52-57% of total lipids. Considering the high-level of GPLs, and their importance in maintaining the integrity and functionality of cell membranes, further utilization of inexpensive tissues, such as viscera and gonads is warranted.
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anticoagulant activity and structural characterization of polysaccharide from abalone Haliotis Discus Hannai ino gonad
Molecules, 2016Co-Authors: Jun Zhao, Jing Feng Yang, Da Yong Zhou, Shuang Song, Weizhou Qiao, Ce Zhu, Shuyin Liu, Bei Wei ZhuAbstract:In this study, we aimed at characterizing the structure and the anticoagulant activity of a polysaccharide fraction (AGP33) isolated from the gonads of Haliotis Discus Hannai Ino. AGP33 was extracted by enzymatic hydrolysis and purified by ion-exchange and gel-filtration chromatography. The backbone fraction of AGP33 (BAGP33), which appeared to contain of mannose, glucose and galactose, was prepared by partial acid hydrolysis. According to methylation and nuclear magnetic resonance (NMR) spectroscopy, the backbone of AGP33 was identified as mainly consisting of 1→3-linked, 1→4-linked, and 1→6-linked monosaccharides. AGP33 is a sulfated polysaccharide with sulfates occur at 3-O- and 4-O-positions. It prolonged thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) compared to a saline control solution in a dosage-dependent manner. AGP33 exhibited an extension (p < 0.01) of APTT compared to the saline group at concentrations higher than 5 μg/mL. AGP33 exhibited higher anticoagulant activity than its desulfated product (AGP33-des) and BAGP33. The results showed that polysaccharide with higher molecular weight and sulfate content demonstrated greater anticoagulant activity.
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effects of heating conditions on fatty acids and volatile compounds in foot muscle of abalone Haliotis Discus Hannai ino
Fisheries Science, 2014Co-Authors: Hongxu Wang, Da Yong Zhou, Lei Qin, Yan Wang, Shuang Song, Xusong Wang, Bei Wei ZhuAbstract:Abalone Haliotis Discus Hannai Ino is a popular delicacy consumed as a luxury food owing to its unique flavor and texture. In this study, we investigated the effects of heating conditions on the fatty acids and volatile compounds in its foot muscle based on gas chromatography–mass spectrometry (GC–MS) and solid-phase microextraction (SPME)–GC–MS. The contents of fatty acids significantly decreased after heating at 80 °C for 2 h. In total, 52 volatile compounds, including aldehydes, aromatic compounds, alkanes, alcohols, ketones, and furans, were detected in the heated samples. Principal component analysis revealed an interaction between heating temperature and time. Heating at 80 °C for 0.5–2 h generated higher contents of volatile compounds. In particular, the contents of hexanal, heptanal, octanal, nonanal, and undecanal mainly derived from autoxidation of fatty acids during heating increased at least fourfold.
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in vitro antioxidant activity of enzymatic hydrolysates prepared from abalone Haliotis Discus Hannai ino viscera
Food and Bioproducts Processing, 2012Co-Authors: Da Yong Zhou, Bei Wei Zhu, Jing Feng Yang, Hai Tao Wu, Dong Mei Li, Lu Qiao, Yoshiyuki MurataAbstract:Pacific abalone (Haliotis Discus Hannai Ino) viscera, which are normally discarded as byproducts, were hydrolyzed with five commercially available proteases, including alkali protease, papain, neutral protease, pepsin, and trypsin. The hydrolysates obtained were fractionated using ultrafiltration membrane bioreactor system. The resulting hydrolysate fractions were investigated for their antioxidant activities, including the scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, the scavenging effect on hydroxyl radical, and the reducing power. Results showed that the hydrolysate fractions were effective antioxidants, and the antioxidant activity of the hydrolysates was related to the enzyme type employed. As byproducts, abalone viscera can be used as a potential source of antioxidant peptides.
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structural analysis and cck releasing activity of a sulphated polysaccharide from abalone Haliotis Discus Hannai ino viscera
Food Chemistry, 2011Co-Authors: Bei Wei Zhu, Jing Feng Yang, Da Yong Zhou, Song Han, George H GreeleyAbstract:Abstract A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone ( Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PW XL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0 kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.
Yoshiyuki Murata - One of the best experts on this subject based on the ideXlab platform.
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antioxidant activity of hydrolysates obtained from scallop patinopecten yessoensis and abalone Haliotis Discus Hannai ino muscle
Food Chemistry, 2012Co-Authors: Da Yong Zhou, Jing Feng Yang, Dong Mei Li, Yue Tang, Yoshiyuki MurataAbstract:Scallop (Patinopecten yessoensis) and abalone (Haliotis Discus Hannai Ino) muscle were hydrolysed with commercially available food-grade proteases. The resulting hydrolysates showed DPPH and hydroxyl radicals scavenging abilities, reducing power, and ferrous ion chelating capacity. The antioxidant activities of hydrolysate of abalone foot muscle (HAFM) increased with increasing incubation time during the whole hydrolysis process in 180 min. Whereas, the antioxidant activities of hydrolysate of scallop adductor muscle (HSAM) increased at initial stage and peaked after 25–30 min of hydrolysis, and then gradually decreased thereafter. Compared with HAFM, HSAM with comparable hydrolysis time contained more free amino acids (FAA) and small-sized peptides (below 500 Da), which may account for the differences in antioxidant activities versus hydrolysis time curves of the two hydrolysates. The above results indicate that limited hydrolysis of proteins can increase their antioxidant activity, whereas extensive hydrolysis can decrease it.
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in vitro antioxidant activity of enzymatic hydrolysates prepared from abalone Haliotis Discus Hannai ino viscera
Food and Bioproducts Processing, 2012Co-Authors: Da Yong Zhou, Bei Wei Zhu, Jing Feng Yang, Hai Tao Wu, Dong Mei Li, Lu Qiao, Yoshiyuki MurataAbstract:Pacific abalone (Haliotis Discus Hannai Ino) viscera, which are normally discarded as byproducts, were hydrolyzed with five commercially available proteases, including alkali protease, papain, neutral protease, pepsin, and trypsin. The hydrolysates obtained were fractionated using ultrafiltration membrane bioreactor system. The resulting hydrolysate fractions were investigated for their antioxidant activities, including the scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, the scavenging effect on hydroxyl radical, and the reducing power. Results showed that the hydrolysate fractions were effective antioxidants, and the antioxidant activity of the hydrolysates was related to the enzyme type employed. As byproducts, abalone viscera can be used as a potential source of antioxidant peptides.
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chemical composition and free radical scavenging activities of a sulphated polysaccharide extracted from abalone gonad Haliotis Discus Hannai ino
Food Chemistry, 2010Co-Authors: Da Yong Zhou, Jing Feng Yang, Dong Mei Li, Xiuping Dong, Tao Li, Yoshiyuki MurataAbstract:Abstract Crude polysaccharides (CPs) were prepared from Haliotis Discus Hannai Ino gonad by protease-hydrolysis combining boiling-methanol extraction. They were purified to homogeneity by gel-filtration chromatography on Sephacryl-S 100 and Sephacryl-S 200, and semi-preparation high performance liquid chromatography (semi-RP-HPLC) equipped with a SOURCE 30Q anion exchange column. The sulphated polysaccharide obtained, named as PAGP, has an average molecular weight (MW) of 12.5 kDa determined by HPLC coupled to a TSK-gel G4000PW XL column. Chemical composition analysis indicated it was composed of carbohydrate (50.7%), sulphated group (31.1%), aminohexose (7.1%), aronic acid (6.2%) and protein (2.0%). The carbohydrate part of PAGP was composed of rhamnose (Rha), fucose (Fuc), and galactose (Gal) in molar ratio of 1.0:1.6:2.3. The protein part of PAGP contained 13 kinds of amino acids. Antioxidant assays demonstrated that PAGP possessed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical scavenging activities than positive controls vitamin C (VC) and vitamin E (VE).
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purification and bioactivity of a sulphated polysaccharide conjugate from viscera of abalone Haliotis Discus Hannai ino
Food and Agricultural Immunology, 2010Co-Authors: Liming Sun, Bei Wei Zhu, Yoshiyuki Murata, Lisha Wang, Xiuping Dong, Rong Xing, Ying DongAbstract:Abstract A sulphated polysaccharide conjugate ACP I was purified from the viscera of abalone Haliotis Discus Hannai Ino, and its immunostimulatory and anti-tumour activities were studied in vivo. Effect of ACP I on immune function was investigated in normal and cyclophosphamide-induced immunosuppressive mice. The anti-tumour effect was investigated by growth inhibition of tumour. Dose-dependent increase in spleen and thymus relative weight, lymphocyte proliferation, phagocytosis of macrophage, natural killer cell activity and antibody production was observed in all the tested mice administered with 10, 20 and 40 mg kg−1 body weight of ACP I. Significant increase of the above function was observed especially in immunosuppressive mice at dose of 40 mg kg−1. A significant inhibition of tumour growth was observed in 10, 20 and 40 mg kg−1 ACP I groups, with inhibitory rates of 40.24, 41.91 and 46.43%, respectively. Results suggested that ACP I might have immunomodulating and anti-tumour potential deserving app...
Jing Feng Yang - One of the best experts on this subject based on the ideXlab platform.
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anticoagulant activity and structural characterization of polysaccharide from abalone Haliotis Discus Hannai ino gonad
Molecules, 2016Co-Authors: Jun Zhao, Jing Feng Yang, Da Yong Zhou, Shuang Song, Weizhou Qiao, Ce Zhu, Shuyin Liu, Bei Wei ZhuAbstract:In this study, we aimed at characterizing the structure and the anticoagulant activity of a polysaccharide fraction (AGP33) isolated from the gonads of Haliotis Discus Hannai Ino. AGP33 was extracted by enzymatic hydrolysis and purified by ion-exchange and gel-filtration chromatography. The backbone fraction of AGP33 (BAGP33), which appeared to contain of mannose, glucose and galactose, was prepared by partial acid hydrolysis. According to methylation and nuclear magnetic resonance (NMR) spectroscopy, the backbone of AGP33 was identified as mainly consisting of 1→3-linked, 1→4-linked, and 1→6-linked monosaccharides. AGP33 is a sulfated polysaccharide with sulfates occur at 3-O- and 4-O-positions. It prolonged thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) compared to a saline control solution in a dosage-dependent manner. AGP33 exhibited an extension (p < 0.01) of APTT compared to the saline group at concentrations higher than 5 μg/mL. AGP33 exhibited higher anticoagulant activity than its desulfated product (AGP33-des) and BAGP33. The results showed that polysaccharide with higher molecular weight and sulfate content demonstrated greater anticoagulant activity.
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antioxidant activity of hydrolysates obtained from scallop patinopecten yessoensis and abalone Haliotis Discus Hannai ino muscle
Food Chemistry, 2012Co-Authors: Da Yong Zhou, Jing Feng Yang, Dong Mei Li, Yue Tang, Yoshiyuki MurataAbstract:Scallop (Patinopecten yessoensis) and abalone (Haliotis Discus Hannai Ino) muscle were hydrolysed with commercially available food-grade proteases. The resulting hydrolysates showed DPPH and hydroxyl radicals scavenging abilities, reducing power, and ferrous ion chelating capacity. The antioxidant activities of hydrolysate of abalone foot muscle (HAFM) increased with increasing incubation time during the whole hydrolysis process in 180 min. Whereas, the antioxidant activities of hydrolysate of scallop adductor muscle (HSAM) increased at initial stage and peaked after 25–30 min of hydrolysis, and then gradually decreased thereafter. Compared with HAFM, HSAM with comparable hydrolysis time contained more free amino acids (FAA) and small-sized peptides (below 500 Da), which may account for the differences in antioxidant activities versus hydrolysis time curves of the two hydrolysates. The above results indicate that limited hydrolysis of proteins can increase their antioxidant activity, whereas extensive hydrolysis can decrease it.
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in vitro antioxidant activity of enzymatic hydrolysates prepared from abalone Haliotis Discus Hannai ino viscera
Food and Bioproducts Processing, 2012Co-Authors: Da Yong Zhou, Bei Wei Zhu, Jing Feng Yang, Hai Tao Wu, Dong Mei Li, Lu Qiao, Yoshiyuki MurataAbstract:Pacific abalone (Haliotis Discus Hannai Ino) viscera, which are normally discarded as byproducts, were hydrolyzed with five commercially available proteases, including alkali protease, papain, neutral protease, pepsin, and trypsin. The hydrolysates obtained were fractionated using ultrafiltration membrane bioreactor system. The resulting hydrolysate fractions were investigated for their antioxidant activities, including the scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, the scavenging effect on hydroxyl radical, and the reducing power. Results showed that the hydrolysate fractions were effective antioxidants, and the antioxidant activity of the hydrolysates was related to the enzyme type employed. As byproducts, abalone viscera can be used as a potential source of antioxidant peptides.
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structural analysis and cck releasing activity of a sulphated polysaccharide from abalone Haliotis Discus Hannai ino viscera
Food Chemistry, 2011Co-Authors: Bei Wei Zhu, Jing Feng Yang, Da Yong Zhou, Song Han, George H GreeleyAbstract:Abstract A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone ( Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PW XL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0 kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.
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chemical composition and free radical scavenging activities of a sulphated polysaccharide extracted from abalone gonad Haliotis Discus Hannai ino
Food Chemistry, 2010Co-Authors: Da Yong Zhou, Jing Feng Yang, Dong Mei Li, Xiuping Dong, Tao Li, Yoshiyuki MurataAbstract:Abstract Crude polysaccharides (CPs) were prepared from Haliotis Discus Hannai Ino gonad by protease-hydrolysis combining boiling-methanol extraction. They were purified to homogeneity by gel-filtration chromatography on Sephacryl-S 100 and Sephacryl-S 200, and semi-preparation high performance liquid chromatography (semi-RP-HPLC) equipped with a SOURCE 30Q anion exchange column. The sulphated polysaccharide obtained, named as PAGP, has an average molecular weight (MW) of 12.5 kDa determined by HPLC coupled to a TSK-gel G4000PW XL column. Chemical composition analysis indicated it was composed of carbohydrate (50.7%), sulphated group (31.1%), aminohexose (7.1%), aronic acid (6.2%) and protein (2.0%). The carbohydrate part of PAGP was composed of rhamnose (Rha), fucose (Fuc), and galactose (Gal) in molar ratio of 1.0:1.6:2.3. The protein part of PAGP contained 13 kinds of amino acids. Antioxidant assays demonstrated that PAGP possessed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical scavenging activities than positive controls vitamin C (VC) and vitamin E (VE).
Guofan Zhang - One of the best experts on this subject based on the ideXlab platform.
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effect of chronic temperature exposure on the immunity of abalone Haliotis Discus Hannai
Aquaculture Research, 2016Co-Authors: Jianfeng Ding, Guofan ZhangAbstract:The effect of temperature on the immune response was studied in the abalone (Haliotis Discus Hannai) reared in seawater at 8, 14 and 26°C for 30 days, with abalone maintained at optimum temperature (20°C) serving as the control. The total haemocyte count (THC) in abalone in the 8°C treatment group was significantly higher than in the control group (P < 0.05). A significantly (P < 0.05) lower phagocytic capacity was observed in the 8 and 14°C treatment groups. Reactive oxygen species (ROS) production in haemocytes of abalone in the 8°C treatment group was significantly (P < 0.05) higher than that in the control group. Real-time RT-PCR analyses indicated that the highest level of serine protease inhibitor (Spi) expression was detected in the 26°C treatment group (P < 0.05), and cysteine proteases inhibitor (Cpi) was also significantly higher in the 8 and 26°C exposure groups. The transcription of the four immune defence genes was induced in all groups after challenge with Vibrio anguillarum. These results indicated that not only the temperature but also the synergy of thermal and bacteria stimulus may affect the immune response of abalone.
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structural characterization and expression analysis of a novel cysteine protease inhibitor from Haliotis Discus Hannai ino
Fish & Shellfish Immunology, 2015Co-Authors: Jianfeng Ding, Guofan ZhangAbstract:The sequence of the cysteine protease inhibitor gene of Haliotis Discus Hannai (designated HdCpi) was determined using the RACE method. The full-length HdCpi cDNA is 1049 bp long, and contains an open reading frame of 813 bp, encoding a 271-amino-acid protein with a calculated molecular mass of 29.83 kDa and an isoelectric point of 8.57. The deduced amino acid sequence of HdCpi contains two cystatin-like domains, and each has the structural features of the cystatin family, including three evolutionarily conserved motifs known to interact with the active sites of cysteine peptidases: the Gly residue at the N-terminus (Gly(65) and Gly(160)), the Gln-X-Val-X-Gly motif (Q(106)IVSO(110) and Q(202)VVAG(206),) and the less conserved motif at the C-terminus ((SW137)-W-136 and A(254)W(255)). Many putative transcription-factor-binding sites involved in the immune system and cancer occur in the promoter region of HdCpi. Quantitative real-time RT-PCR detected HdCpi expression in all the tissues examined and in the gills of abalone challenged with the bacterium Vibrio anguillarum. HdCpi transcripts were expressed in the mantle, gill, digestive tract, hemocytes, and muscle, and increased HdCpi expression was observed after bacterial stimulation. These results suggest that HdCpi is a biologically active protease inhibitor that is likely to be involved in the antibacterial response of the abalone. (C) 2014 Elsevier Ltd. All rights reserved.
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suitability of cage culture for pacific abalone Haliotis Discus Hannai ino production in china
Aquaculture Research, 2013Co-Authors: Guofan ZhangAbstract:Pacific abalone (Haliotis Discus Hannai Ino) aquaculture is a thriving industry in China. This study describes a novel submerged cage culture system for abalone rearing in Fujian, South China. The cage consisted of five vertical slots that were oriented perpendicular to the flow of water. The slots were separated by six vertically connected plastic plates for abalone attachment and shelter at the bottom of the cage. Experiment 1 was designed to determine the appropriate stocking density at the start of the abalone sea-based production cycle. Eight-month-old hatchery reared and size-graded juveniles were transferred to the sea-based culture system. For different stocking densities, shell length of juveniles obtained in this novel culture system on 2, 3.5 and 5months, respectively, was compared with shell lengths obtained in a traditionally multi-tier basket culture system. In Experiment 2, daily growth rates (DGRs) in shell length and biomass in terms of wet weight of 2-year-old abalones reared in cage and tiered basket culture systems were compared over a 6-month period. Results of Experiment 1 showed that growth of abalone in the cage culture system is density-dependent; the mean final shell length of juveniles obtained was 6.715.9% higher than in tiered baskets system even at the same initial stocking density. In Experiment 2, DGRs in shell length of 53.8378.38mday1 obtained in cage system were significantly higher than that in tiered baskets (P<0.01). And in terms of wet weight biomass, it was 1.483.01 times higher in the cage system compared with the traditional system. Abalone survival was more than 87.5% in both culture systems in both experiments. Advantages of the newly established cage culture system included better growth performance of the animals reared and potential improvement of rearing conditions, such as improved water flow velocity and dissolved oxygen.
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heterosis and combining ability a diallel cross of three geographically isolated populations of pacific abalone Haliotis Discus Hannai ino
Chinese Journal of Oceanology and Limnology, 2010Co-Authors: Xiao Liu, Yuewen Deng, Guofan ZhangAbstract:We conducted a complete diallel cross among three geographically isolated populations of Pacific abalone Haliotis Discus Hannai Ino to determine the heterosis and the combining ability of growth traits at the spat stage. The three populations were collected from Qingdao (Q) and Dalian (D) in China, and Miyagi (M) in Japan. We measured the shell length, shell width, and total weight. The magnitude of the general combining ability (GCA) variance was more pronounced than the specific combining ability (SCA) variance, which is evidenced by both the ratio of the genetic component in total variation and the GCA/SCA values. The component variances of GCA and SCA were significant for all three traits (P<0.05), indicating the importance of additive and non-additive genetic effects in determining the expression of these traits. The reciprocal maternal effects (RE) were also significant for these traits (P<0.05). Our results suggest that population D was the best general combiner in breeding programs to improve growth traits. The DM cross had the highest heterosis values for all three traits.
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mining expressed sequences for single nucleotide polymorphisms in pacific abalone Haliotis Discus Hannai
Aquaculture Research, 2009Co-Authors: Xiao Liu, Guofan ZhangAbstract:Although single nucleotide polymorphisms (SNPs) are important resources for population genetics, pedigree analysis and genomic mapping, such loci have not been reported in Pacific abalone so far. In this study, a bioinformatics strategy was adopted to discover SNPs within the expressed sequences (ESTs) of Pacific abalone, Haliotis Discus Hannai, and furthermore, polymerase chain reaction direct sequencing (PCR-DS) and allele-specific PCR (AS-PCR) were used for SNPs detection and genotype scoring respectively. A total of 5893 ESTs were assembled and 302 putative SNPs were identified. The average density of SNPs in ESTs was 1%. Fifty-two sets of sequencing primers were designed from SNPs flanking ESTs to amplify the genomic DNA, and 13 could generate products of expected size. Polymerase chain reaction direct sequencing of the amplification products from pooled DNA samples revealed 40 polymorphic SNP loci. Using a modified tetra-primer AS-PCR, seven mitochondrial and six nuclear SNPs were typed and characterized among 37 wild abalones. In conclusion, it is feasible to discover SNPs from number limited ESTs and the AS-PCR as a simple, robust and reliable assay could be a primary method for small- and medium-scale SNPs detection in abalones as well as other non-model organisms.
