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Maudy Th Smith - One of the best experts on this subject based on the ideXlab platform.
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kloeckera janke 1928
The Yeasts (Fifth Edition), 2011Co-Authors: Neza Cadez, Maudy Th SmithAbstract:Publisher Summary This chapter studies the genus Kloeckera. In the asexual reproduction budding is bipolar. Blastoconidia are formed in basipetal succession on a broad base. Cells are apiculate, ovoid to long-ovoid, or elongate. Pseudohyphae may be present, usually rudimentary and rarely well-developed. Colonies are smooth and cream in color. In sexual reproduction it is found that the ascospores are not produced. Ascosporic species are assigned to the teleomorph genus Hanseniaspora. The chapter also discusses physiology/biochemistry and phylogenetic placement of the genus in which glucose is fermented and nitrate is not assimilated. Myo-Inositol and pantothenate are required for growth. Acetic acid is not produced, coenzyme Q contains six isoprene units, and the diazonium blue B reaction is negative. The type species taken is Kloeckera apiculata. The six anamorphic Kloeckera species with six teleomorphic species in Hanseniaspora from comparisons of DNA homology are correlated.
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Hanseniaspora Zikes (1912)
The Yeasts, 2011Co-Authors: Neza Cadez, Maudy Th SmithAbstract:Publisher Summary This chapter studies the genus Hanseniaspora. In the determination of the asexual reproduction, it is seen that budding is bipolar, and blastoconidia are formed in basipetal succession on a broad base. Cells are apiculate and ovoid to long-ovoid or elongate. Pseudohyphae may be present but are rarely well-developed. Colonies are smooth and cream in color. The anamorph genus is Kloeckera. In sexual reproduction it is found that asci, which are persistent or deliquescent, form one to four ascospores that are hat- or helmet-shaped, spherical with warts, spherical, and smooth with an equatorial ledge or spherical and warty with an equatorial ledge. Asci arise without conjugation. The chapter also discusses physiology/biochemistry and phylogenetic placement of the genus. The type species taken is Hanseniaspora valbyensis. It also describes the five genetically distinct Hanseniaspora species, H. meyeri, H. clermontiae, H. lachancei, H. opuntiae, and H. pseudoguilliermondii, which are recognized by rapid genomic fingerprinting and DNA reassociation studies.
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phylogenetic placement of Hanseniaspora kloeckera species using multigene sequence analysis with taxonomic implications descriptions of Hanseniaspora pseudoguilliermondii sp nov and Hanseniaspora occidentalis var citrica var nov
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Neza Cadez, Peter Raspor, Maudy Th SmithAbstract:Two protein-coding genes, actin and translation elongation factor-1α (EF-1α), as well as two ribosomal gene regions, D1/D2 domains of the large subunit and both internal transcribed spacers including the 5.8S gene region, were evaluated regarding their usefulness for reconstruction of phylogenetic relationships in the Hanseniaspora–Kloeckera species group. This included analyses of sequence divergence values, heterogeneity of evolutionary rates and the reliability of the inferred trees. Both protein-coding genes showed greater capacities to resolve at the strain level and between the closely related species of Hanseniaspora–Kloeckera, compared with the ribosomal gene regions. However, to obtain a fully resolved and reliable phylogenetic tree that reflected the biological relationships it was necessary to combine three congruent sequence datasets. The novel species Hanseniaspora pseudoguilliermondii sp. nov. (type strain CBS 8772T) is described as a result of the application of various molecular approaches to delimit species. Furthermore, incongruent gene genealogies of genetically divergent strains of Hanseniaspora occidentalis, as determined by amplified fragment length polymorphism analysis and DNA–DNA reassociation measurements, indicated the presence of two novel varieties, H. occidentalis var. occidentalis (type strain CBS 2592T) and H. occidentalis var. citrica var. nov. (type strain CBS 6783T), which could be distinguished by habitat preference.
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Phylogenetic placement of Hanseniaspora–Kloeckera species using multigene sequence analysis with taxonomic implications: descriptions of Hanseniaspora pseudoguilliermondii sp. nov. and Hanseniaspora occidentalis var. citrica var. nov.
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Neza Cadez, Peter Raspor, Maudy Th SmithAbstract:Two protein-coding genes, actin and translation elongation factor-1α (EF-1α), as well as two ribosomal gene regions, D1/D2 domains of the large subunit and both internal transcribed spacers including the 5.8S gene region, were evaluated regarding their usefulness for reconstruction of phylogenetic relationships in the Hanseniaspora–Kloeckera species group. This included analyses of sequence divergence values, heterogeneity of evolutionary rates and the reliability of the inferred trees. Both protein-coding genes showed greater capacities to resolve at the strain level and between the closely related species of Hanseniaspora–Kloeckera, compared with the ribosomal gene regions. However, to obtain a fully resolved and reliable phylogenetic tree that reflected the biological relationships it was necessary to combine three congruent sequence datasets. The novel species Hanseniaspora pseudoguilliermondii sp. nov. (type strain CBS 8772T) is described as a result of the application of various molecular approaches to delimit species. Furthermore, incongruent gene genealogies of genetically divergent strains of Hanseniaspora occidentalis, as determined by amplified fragment length polymorphism analysis and DNA–DNA reassociation measurements, indicated the presence of two novel varieties, H. occidentalis var. occidentalis (type strain CBS 2592T) and H. occidentalis var. citrica var. nov. (type strain CBS 6783T), which could be distinguished by habitat preference.
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Molecular identification and genetic diversity within species of the genera and
Fems Yeast Research, 2006Co-Authors: Neza Cadez, Arnaud Decock, Teun Boekhout, Peter Raspor, Maudy Th SmithAbstract:Three molecular methods, RAPD-PCR analysis, electrophoretic karyotyping and RFLP of the PCR-amplified ITS regions (ITS1, ITS2 and the intervening 5.8S rDNA), were studied for accurate identification of Hanseniaspora and Kloeckera species as well as for determining inter- and intraspecific relationships of 74 strains isolated from different sources and/or geographically distinct regions. Of these three methods, PCR-RFLP analysis of ITS regions with restriction enzymes DdeI and HinfI is proposed as a rapid identification method to discriminate unambiguously between all six Hanseniaspora species and the single non-ascospore-forming apiculate yeast species Kloeckera lindneri. Electrophoretic karyotyping produced chromosomal profiles by which the seven species could be divided into four groups sharing similar karyotypes. Although most of the 60 strains examined exhibited a common species-specific pattern, a different degree of chromosomal-length polymorphism and a variable number of chromosomal DNA fragments were observed within species. Cluster analysis of the combined RAPD-PCR fingerprints obtained with one 10-mer primer, two microsatellite primers and one minisatellite primer generated clusters which with a few exceptions are in agreement with the groups as earlier recognized in DNA–DNA homology studies.
Neza Cadez - One of the best experts on this subject based on the ideXlab platform.
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Hanseniaspora Zikes (1912)
The Yeasts, 2011Co-Authors: Neza Cadez, Maudy Th SmithAbstract:Publisher Summary This chapter studies the genus Hanseniaspora. In the determination of the asexual reproduction, it is seen that budding is bipolar, and blastoconidia are formed in basipetal succession on a broad base. Cells are apiculate and ovoid to long-ovoid or elongate. Pseudohyphae may be present but are rarely well-developed. Colonies are smooth and cream in color. The anamorph genus is Kloeckera. In sexual reproduction it is found that asci, which are persistent or deliquescent, form one to four ascospores that are hat- or helmet-shaped, spherical with warts, spherical, and smooth with an equatorial ledge or spherical and warty with an equatorial ledge. Asci arise without conjugation. The chapter also discusses physiology/biochemistry and phylogenetic placement of the genus. The type species taken is Hanseniaspora valbyensis. It also describes the five genetically distinct Hanseniaspora species, H. meyeri, H. clermontiae, H. lachancei, H. opuntiae, and H. pseudoguilliermondii, which are recognized by rapid genomic fingerprinting and DNA reassociation studies.
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kloeckera janke 1928
The Yeasts (Fifth Edition), 2011Co-Authors: Neza Cadez, Maudy Th SmithAbstract:Publisher Summary This chapter studies the genus Kloeckera. In the asexual reproduction budding is bipolar. Blastoconidia are formed in basipetal succession on a broad base. Cells are apiculate, ovoid to long-ovoid, or elongate. Pseudohyphae may be present, usually rudimentary and rarely well-developed. Colonies are smooth and cream in color. In sexual reproduction it is found that the ascospores are not produced. Ascosporic species are assigned to the teleomorph genus Hanseniaspora. The chapter also discusses physiology/biochemistry and phylogenetic placement of the genus in which glucose is fermented and nitrate is not assimilated. Myo-Inositol and pantothenate are required for growth. Acetic acid is not produced, coenzyme Q contains six isoprene units, and the diazonium blue B reaction is negative. The type species taken is Kloeckera apiculata. The six anamorphic Kloeckera species with six teleomorphic species in Hanseniaspora from comparisons of DNA homology are correlated.
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phylogenetic placement of Hanseniaspora kloeckera species using multigene sequence analysis with taxonomic implications descriptions of Hanseniaspora pseudoguilliermondii sp nov and Hanseniaspora occidentalis var citrica var nov
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Neza Cadez, Peter Raspor, Maudy Th SmithAbstract:Two protein-coding genes, actin and translation elongation factor-1α (EF-1α), as well as two ribosomal gene regions, D1/D2 domains of the large subunit and both internal transcribed spacers including the 5.8S gene region, were evaluated regarding their usefulness for reconstruction of phylogenetic relationships in the Hanseniaspora–Kloeckera species group. This included analyses of sequence divergence values, heterogeneity of evolutionary rates and the reliability of the inferred trees. Both protein-coding genes showed greater capacities to resolve at the strain level and between the closely related species of Hanseniaspora–Kloeckera, compared with the ribosomal gene regions. However, to obtain a fully resolved and reliable phylogenetic tree that reflected the biological relationships it was necessary to combine three congruent sequence datasets. The novel species Hanseniaspora pseudoguilliermondii sp. nov. (type strain CBS 8772T) is described as a result of the application of various molecular approaches to delimit species. Furthermore, incongruent gene genealogies of genetically divergent strains of Hanseniaspora occidentalis, as determined by amplified fragment length polymorphism analysis and DNA–DNA reassociation measurements, indicated the presence of two novel varieties, H. occidentalis var. occidentalis (type strain CBS 2592T) and H. occidentalis var. citrica var. nov. (type strain CBS 6783T), which could be distinguished by habitat preference.
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Phylogenetic placement of Hanseniaspora–Kloeckera species using multigene sequence analysis with taxonomic implications: descriptions of Hanseniaspora pseudoguilliermondii sp. nov. and Hanseniaspora occidentalis var. citrica var. nov.
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Neza Cadez, Peter Raspor, Maudy Th SmithAbstract:Two protein-coding genes, actin and translation elongation factor-1α (EF-1α), as well as two ribosomal gene regions, D1/D2 domains of the large subunit and both internal transcribed spacers including the 5.8S gene region, were evaluated regarding their usefulness for reconstruction of phylogenetic relationships in the Hanseniaspora–Kloeckera species group. This included analyses of sequence divergence values, heterogeneity of evolutionary rates and the reliability of the inferred trees. Both protein-coding genes showed greater capacities to resolve at the strain level and between the closely related species of Hanseniaspora–Kloeckera, compared with the ribosomal gene regions. However, to obtain a fully resolved and reliable phylogenetic tree that reflected the biological relationships it was necessary to combine three congruent sequence datasets. The novel species Hanseniaspora pseudoguilliermondii sp. nov. (type strain CBS 8772T) is described as a result of the application of various molecular approaches to delimit species. Furthermore, incongruent gene genealogies of genetically divergent strains of Hanseniaspora occidentalis, as determined by amplified fragment length polymorphism analysis and DNA–DNA reassociation measurements, indicated the presence of two novel varieties, H. occidentalis var. occidentalis (type strain CBS 2592T) and H. occidentalis var. citrica var. nov. (type strain CBS 6783T), which could be distinguished by habitat preference.
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Molecular identification and genetic diversity within species of the genera and
Fems Yeast Research, 2006Co-Authors: Neza Cadez, Arnaud Decock, Teun Boekhout, Peter Raspor, Maudy Th SmithAbstract:Three molecular methods, RAPD-PCR analysis, electrophoretic karyotyping and RFLP of the PCR-amplified ITS regions (ITS1, ITS2 and the intervening 5.8S rDNA), were studied for accurate identification of Hanseniaspora and Kloeckera species as well as for determining inter- and intraspecific relationships of 74 strains isolated from different sources and/or geographically distinct regions. Of these three methods, PCR-RFLP analysis of ITS regions with restriction enzymes DdeI and HinfI is proposed as a rapid identification method to discriminate unambiguously between all six Hanseniaspora species and the single non-ascospore-forming apiculate yeast species Kloeckera lindneri. Electrophoretic karyotyping produced chromosomal profiles by which the seven species could be divided into four groups sharing similar karyotypes. Although most of the 60 strains examined exhibited a common species-specific pattern, a different degree of chromosomal-length polymorphism and a variable number of chromosomal DNA fragments were observed within species. Cluster analysis of the combined RAPD-PCR fingerprints obtained with one 10-mer primer, two microsatellite primers and one minisatellite primer generated clusters which with a few exceptions are in agreement with the groups as earlier recognized in DNA–DNA homology studies.
Peter Raspor - One of the best experts on this subject based on the ideXlab platform.
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Hanseniaspora nectarophila sp. nov., a yeast species isolated from ephemeral flowers.
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Neža Čadež, Peter Raspor, Fernando C. Pagnocca, Carlos A. RosaAbstract:Seven apiculate yeast strains that were isolated from the flowers of Syphocampylus corymbiferus Pohl in Brazil are genetically, morphologically and phenotypically distinct from recognized species of the genera Hanseniaspora and Kloeckera. Genetic discontinuities between the novel strains and their closest relatives were found using a networking approach based on the concatenated sequences of the rRNA gene (internal transcribed spacer and D1/D2 of the LSU), and the protein-coding genes for actin and translation elongation factor-1α. Phylogenetic analysis based on the rRNA and the actin gene placed the novel species represented by the strains in close relationship to Hanseniaspora meyeri and Hanseniaspora clermontiae. PCR fingerprinting with microsatellite primers confirmed the genetic heterogeneity of the novel species. The name Hanseniaspora nectarophila sp. nov. is proposed, with UFMG POG a.1T ( = ZIM 2311T = CBS 13383T) as the type strain; MycoBank no. MB807210. As the current description of the genus does not allow the presence of multilateral budding, an emended diagnosis of the genus Hanseniaspora Zikes is proposed.
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phylogenetic placement of Hanseniaspora kloeckera species using multigene sequence analysis with taxonomic implications descriptions of Hanseniaspora pseudoguilliermondii sp nov and Hanseniaspora occidentalis var citrica var nov
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Neza Cadez, Peter Raspor, Maudy Th SmithAbstract:Two protein-coding genes, actin and translation elongation factor-1α (EF-1α), as well as two ribosomal gene regions, D1/D2 domains of the large subunit and both internal transcribed spacers including the 5.8S gene region, were evaluated regarding their usefulness for reconstruction of phylogenetic relationships in the Hanseniaspora–Kloeckera species group. This included analyses of sequence divergence values, heterogeneity of evolutionary rates and the reliability of the inferred trees. Both protein-coding genes showed greater capacities to resolve at the strain level and between the closely related species of Hanseniaspora–Kloeckera, compared with the ribosomal gene regions. However, to obtain a fully resolved and reliable phylogenetic tree that reflected the biological relationships it was necessary to combine three congruent sequence datasets. The novel species Hanseniaspora pseudoguilliermondii sp. nov. (type strain CBS 8772T) is described as a result of the application of various molecular approaches to delimit species. Furthermore, incongruent gene genealogies of genetically divergent strains of Hanseniaspora occidentalis, as determined by amplified fragment length polymorphism analysis and DNA–DNA reassociation measurements, indicated the presence of two novel varieties, H. occidentalis var. occidentalis (type strain CBS 2592T) and H. occidentalis var. citrica var. nov. (type strain CBS 6783T), which could be distinguished by habitat preference.
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Phylogenetic placement of Hanseniaspora–Kloeckera species using multigene sequence analysis with taxonomic implications: descriptions of Hanseniaspora pseudoguilliermondii sp. nov. and Hanseniaspora occidentalis var. citrica var. nov.
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Neza Cadez, Peter Raspor, Maudy Th SmithAbstract:Two protein-coding genes, actin and translation elongation factor-1α (EF-1α), as well as two ribosomal gene regions, D1/D2 domains of the large subunit and both internal transcribed spacers including the 5.8S gene region, were evaluated regarding their usefulness for reconstruction of phylogenetic relationships in the Hanseniaspora–Kloeckera species group. This included analyses of sequence divergence values, heterogeneity of evolutionary rates and the reliability of the inferred trees. Both protein-coding genes showed greater capacities to resolve at the strain level and between the closely related species of Hanseniaspora–Kloeckera, compared with the ribosomal gene regions. However, to obtain a fully resolved and reliable phylogenetic tree that reflected the biological relationships it was necessary to combine three congruent sequence datasets. The novel species Hanseniaspora pseudoguilliermondii sp. nov. (type strain CBS 8772T) is described as a result of the application of various molecular approaches to delimit species. Furthermore, incongruent gene genealogies of genetically divergent strains of Hanseniaspora occidentalis, as determined by amplified fragment length polymorphism analysis and DNA–DNA reassociation measurements, indicated the presence of two novel varieties, H. occidentalis var. occidentalis (type strain CBS 2592T) and H. occidentalis var. citrica var. nov. (type strain CBS 6783T), which could be distinguished by habitat preference.
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Molecular identification and genetic diversity within species of the genera and
Fems Yeast Research, 2006Co-Authors: Neza Cadez, Arnaud Decock, Teun Boekhout, Peter Raspor, Maudy Th SmithAbstract:Three molecular methods, RAPD-PCR analysis, electrophoretic karyotyping and RFLP of the PCR-amplified ITS regions (ITS1, ITS2 and the intervening 5.8S rDNA), were studied for accurate identification of Hanseniaspora and Kloeckera species as well as for determining inter- and intraspecific relationships of 74 strains isolated from different sources and/or geographically distinct regions. Of these three methods, PCR-RFLP analysis of ITS regions with restriction enzymes DdeI and HinfI is proposed as a rapid identification method to discriminate unambiguously between all six Hanseniaspora species and the single non-ascospore-forming apiculate yeast species Kloeckera lindneri. Electrophoretic karyotyping produced chromosomal profiles by which the seven species could be divided into four groups sharing similar karyotypes. Although most of the 60 strains examined exhibited a common species-specific pattern, a different degree of chromosomal-length polymorphism and a variable number of chromosomal DNA fragments were observed within species. Cluster analysis of the combined RAPD-PCR fingerprints obtained with one 10-mer primer, two microsatellite primers and one minisatellite primer generated clusters which with a few exceptions are in agreement with the groups as earlier recognized in DNA–DNA homology studies.
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Hanseniaspora meyeri sp. nov., Hanseniaspora clermontiae sp. nov., Hanseniaspora lachancei sp. nov. and Hanseniaspora opuntiae sp. nov., novel apiculate yeast species.
International journal of systematic and evolutionary microbiology, 2003Co-Authors: Neza Cadez, Peter Raspor, Gé A Poot, Maudy Th SmithAbstract:Fourteen apiculate yeast strains isolated from various sources in South Africa, North America and the Hawaiian islands were found to be genetically divergent from other Hanseniaspora-Kloeckera species by using randomly amplified polymorphic DNA (RAPD)-PCR. After cluster analysis of the RAPD-PCR fingerprints, five groups were recognized. DNA reassociation values among representatives of these groups and strains of Hanseniaspora-Kloeckera species revealed that the strains represent five novel species. Four are described here as novel species of Hanseniaspora: Hanseniaspora meyeri sp. nov. (type CBS 8734(T)), Hanseniaspora clermontiae sp. nov. (type CBS 8821(T)), Hanseniaspora lachancei sp. nov. (type CBS 8818(T)) and Hanseniaspora opuntiae sp. nov. (type CBS 8733(T)). The fifth novel species, which is represented by only a single strain, CBS 8772, is not introduced as a new taxon. Phylogenetic analyses of the D1/D2 region of the 26S rDNA and internal transcribed spacer (ITS) regions with 5.8S rDNA sequences placed H. meyeri, H. clermontiae, H. lachancei, H. opuntiae and strain CBS 8772 close to Hanseniaspora uvarum and Hanseniaspora guilliermondii. The key characteristics for standard physiological identification of H. clermontiae and H. lachancei were respectively maximal growth temperature and assimilation of 2-keto-D-gluconate. However, physiological characteristics did not allow the distinction of H. opuntiae and strain CBS 8772 from H. guilliermondii or H. meyeri from H. uvarum. These three novel taxa can be identified by either ITS sequencing or PCR-RFLP of ITS regions using restriction enzymes MboII and HinfI.
Michèle Guilloux-benatier - One of the best experts on this subject based on the ideXlab platform.
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The sensitivity of yeasts and yeasts-like fungi to copper and sulfur could explain lower yeast biodiversity in organic vineyards.
Fems Yeast Research, 2017Co-Authors: Cédric Grangeteau, Vanessa David, Alexandre Hervé, Michèle Guilloux-benatier, Sandrine RousseauxAbstract:Although differences in yeast biodiversity have often been found between vineyards subjected to organic protection or conventional protection, little is known about the effect of copper and sulfur fungicides (the only fungicides allowed in organic farming) on yeast populations. The sensitivity to copper and sulfur of 158 yeast isolates of seven different species (Aureobasidium pullulans, Hanseniaspora guilliermondii, Hanseniaspora uvarum, Metschnikowia sp., Pichia membranifaciens, Saccharomyces cerevisiae and Starmerella bacillaris) was evaluated. The species Aureobasidium pullulans and Starmerella bacillaris appeared to be more resistant to copper than the other species tested. The species Aureobasidium pullulans, Hanseniaspora guilliermondii and Metschnikowia sp. had the highest sulfur resistance. Thus, only isolates of the species Aureobasidium pullulans exhibited high resistance to both antifungal agents. These results may explain the lower diversity of yeasts present on berries and the strong dominance of the species Aureobasidium pullulans for vineyards protected by copper or sulfur-based fungicides compared to other vineyards reported by several studies.
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Persistence of Two Non-Saccharomyces Yeasts (Hanseniaspora and Starmerella) in the Cellar
Frontiers in Microbiology, 2016Co-Authors: Cédric Grangeteau, Sandrine Rousseaux, Christian Von Wallbrunn, Daniel Gerhards, Hervé Alexandre, Michèle Guilloux-benatierAbstract:Different genera and/or species of yeasts present on grape berries, in musts and wines are widely described. Nevertheless, the community of non-Saccharomyces yeasts present in the cellar is still given little attention. Thus it is not known if the cellar is a real ecological niche for these yeasts or if it is merely a transient habitat for populations brought in by grape berries during the winemaking period. This study focused on three species of non-Saccharomyces yeasts commonly encountered during vinification: Starmerella bacillaris (synonymy with Candida zemplinina), Hanseniaspora guilliermondii and Hanseniaspora uvarum. More than 1200 isolates were identified at the strain level by FT-IR spectroscopy (207 different FTIR strain pattern). Only a small proportion of non-Saccharomyces yeasts present in musts came directly from grape berries for the three species studied. Some strains were found in the must in two consecutive years and some of them were also found in the cellar environment before the arrival of the harvest of second vintage. This study demonstrates for the first time the persistence of non-Saccharomyces yeast strains from year to year in the cellar. Sulfur dioxide can affect yeast populations in the must and therefore their persistence in the cellar environment.
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Diversity of yeast strains of the genus Hanseniaspora in the winery environment: What is their involvement in grape must fermentation?
Food Microbiology, 2015Co-Authors: Cédric Grangeteau, Sandrine Rousseaux, Christian Von Wallbrunn, Daniel Gerhards, Hervé Alexandre, Michèle Guilloux-benatierAbstract:Isolated yeast populations of Chardonnay grape must during spontaneous fermentation were compared to those isolated on grape berries and in a winery environment before the arrival of the harvest (air, floor, winery equipment) and in the air through time. Two genera of yeast, Hanseniaspora and Saccharomyces, were isolated in grape must and in the winery environment before the arrival of the harvest but not on grape berries. The genus Hanseniaspora represented 27% of isolates in the must and 35% of isolates in the winery environment. The isolates of these two species were discriminated at the strain level by Fourier transform infrared spectroscopy. The diversity of these strains observed in the winery environment (26 strains) and in must (12 strains) was considerable. 58% of the yeasts of the genus Hanseniaspora isolated in the must corresponded to strains present in the winery before the arrival of the harvest. Although the proportion and number of strains of the genus Hanseniaspora decreased during fermentation, some strains, all from the winery environment, subsisted up to 5% ethanol content. This is the first time that the implantation in grape must of populations present in the winery environment has been demonstrated for a non-Saccharomyces genus.
Francisco Carrau - One of the best experts on this subject based on the ideXlab platform.
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Comparison of the Glycolytic and Alcoholic Fermentation Pathways of Hanseniaspora vineae with Saccharomyces cerevisiae Wine Yeasts
Fermentation, 2020Co-Authors: Maria Jose Valera, Eduardo Boido, Eduardo Dellacassa, Francisco CarrauAbstract:Hanseniaspora species can be isolated from grapes and grape musts, but after the initiation of spontaneous fermentation, they are displaced by Saccharomyces cerevisiae. Hanseniaspora vineae is particularly valuable since this species improves the flavour of wines and has an increased capacity to ferment relative to other apiculate yeasts. Genomic, transcriptomic, and metabolomic studies in H. vineae have enhanced our understanding of its potential utility within the wine industry. Here, we compared gene sequences of 12 glycolytic and fermentation pathway enzymes from five sequenced Hanseniaspora species and S. cerevisiae with the corresponding enzymes encoded within the two sequenced H. vineae genomes. Increased levels of protein similarity were observed for enzymes of H. vineae and S. cerevisiae, relative to the remaining Hanseniaspora species. Key differences between H. vineae and H. uvarum pyruvate kinase enzymes might explain observed differences in fermentative capacity. Further, the presence of eight putative alcohol dehydrogenases, invertase activity, and sulfite tolerance are distinctive characteristics of H. vineae, compared to other Hanseniaspora species. The definition of two clear technological groups within the Hanseniaspora genus is discussed within the slow and fast evolution concept framework previously discovered in these apiculate yeasts.
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oenological impact of the Hanseniaspora kloeckera yeast genus on wines a review
Fermentation, 2018Co-Authors: Valentina Martin, Maria Jose Valera, Karina Toscano Medina, Eduardo Boido, Francisco CarrauAbstract:Apiculate yeasts of the genus Hanseniaspora/Kloeckera are the main species present on mature grapes and play a significant role at the beginning of fermentation, producing enzymes and aroma compounds that expand the diversity of wine color and flavor. Ten species of the genus Hanseniaspora have been recovered from grapes and are associated in two groups: H. valbyensis, H. guilliermondii, H. uvarum, H. opuntiae, H. thailandica, H. meyeri, and H. clermontiae; and H. vineae, H. osmophila, and H. occidentalis. This review focuses on the application of some strains belonging to this genus in co-fermentation with Saccharomyces cerevisiae that demonstrates their positive contribution to winemaking. Some consistent results have shown more intense flavors and complex, full-bodied wines, compared with wines produced by the use of S. cerevisiae alone. Recent genetic and physiologic studies have improved the knowledge of the Hanseniaspora/Kloeckera species. Significant increases in acetyl esters, benzenoids, and sesquiterpene flavor compounds, and relative decreases in alcohols and acids have been reported, due to different fermentation pathways compared to conventional wine yeasts.
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Oenological Impact of the Hanseniaspora/Kloeckera Yeast Genus on Wines—A Review
Fermentation, 2018Co-Authors: Valentina Martin, Maria Jose Valera, Karina Toscano Medina, Eduardo Boido, Francisco CarrauAbstract:Apiculate yeasts of the genus Hanseniaspora/Kloeckera are the main species present on mature grapes and play a significant role at the beginning of fermentation, producing enzymes and aroma compounds that expand the diversity of wine color and flavor. Ten species of the genus Hanseniaspora have been recovered from grapes and are associated in two groups: H. valbyensis, H. guilliermondii, H. uvarum, H. opuntiae, H. thailandica, H. meyeri, and H. clermontiae; and H. vineae, H. osmophila, and H. occidentalis. This review focuses on the application of some strains belonging to this genus in co-fermentation with Saccharomyces cerevisiae that demonstrates their positive contribution to winemaking. Some consistent results have shown more intense flavors and complex, full-bodied wines, compared with wines produced by the use of S. cerevisiae alone. Recent genetic and physiologic studies have improved the knowledge of the Hanseniaspora/Kloeckera species. Significant increases in acetyl esters, benzenoids, and sesquiterpene flavor compounds, and relative decreases in alcohols and acids have been reported, due to different fermentation pathways compared to conventional wine yeasts.
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Effect of yeast assimilable nitrogen on the synthesis of phenolic aroma compounds by Hanseniaspora vineae strains
Yeast (Chichester England), 2016Co-Authors: Valentina Martin, Eduardo Boido, Eduardo Dellacassa, Facundo Giorello, Albert Mas, Francisco CarrauAbstract:In several grape varieties, the dominating aryl alkyl alcohols found are the volatile group of phenylpropanoid-related compounds, such as glycosylated benzyl and 2-phenylethyl alcohol, which contribute to wine with floral and fruity aromas after being hydrolysed during fermentation. Saccharomyces cerevisiae is largely recognized as the main agent in grape must fermentation, but yeast strains belonging to other genera, including Hanseniaspora, are known to predominate during the first stages of alcoholic fermentation. Although non-Saccharomyces yeast strains have a well-recognized genetic diversity, understanding of their impact on wine flavour richness is still emerging. In this study, 11 Hansenisapora vineae strains were used to ferment a chemically defined simil-grape fermentation medium, resembling the nutrient composition of grape juice but devoid of grape-derived secondary metabolites. GC-MS analysis was performed to determine volatile compounds in the produced wines. Our results showed that benzyl alcohol, benzyl acetate and 2-phenylethyl acetate are significantly synthesized by H. vineae strains. Levels of these compounds found in fermentations with 11 H. vineae different strains were one or two orders of magnitude higher than those measured in fermentations with a known S. cerevisiae wine strain. The implications for winemaking in response to the negative correlation of benzyl alcohol, benzyl acetate and 2-phenylethyl acetate production with yeast assimilable nitrogen concentrations are discussed. Copyright © 2016 John Wiley & Sons, Ltd.
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genome sequence of the native apiculate wine yeast Hanseniaspora vineae t02 19af
Genome Announcements, 2014Co-Authors: Facundo Giorello, Valentina Salzman, Laura Camesasca, Karina Medina, Luisa Berná, Gonzalo Greif, Carlos Robello, Carina Gaggero, Pablo S. Aguilar, Francisco CarrauAbstract:ABSTRACT The use of novel yeast strains for winemaking improves quality and provides variety including subtle characteristic differences in fine wines. Here we report the first genome of a yeast strain native to Uruguay, Hanseniaspora vineae T02/19AF, which has been shown to positively contribute to aroma and wine quality.
