The Experts below are selected from a list of 12111 Experts worldwide ranked by ideXlab platform
Marc Van Ranst - One of the best experts on this subject based on the ideXlab platform.
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A lethal disease model for New World Hantaviruses using immunosuppressed Syrian hamsters.
PLoS neglected tropical diseases, 2017Co-Authors: Valentijn Vergote, Lies Laenen, Bert Vanmechelen, Marc Van Ranst, Erik Verbeken, Piet MaesAbstract:Background Hantavirus, the hemorrhagic causative agent of two clinical diseases, is found worldwide with variation in severity, incidence and mortality. The most lethal Hantaviruses are found on the American continent where the most prevalent viruses like Andes virus and Sin Nombre virus are known to cause Hantavirus pulmonary syndrome. New World Hantavirus infection of immunocompetent hamsters results in an asymptomatic infection except for Andes virus and Maporal virus; the only Hantaviruses causing a lethal disease in immunocompetent Syrian hamsters mimicking Hantavirus pulmonary syndrome in humans. Methodology/Principal findings Hamsters, immunosuppressed with dexamethasone and cyclophosphamide, were infected intramuscularly with different New World Hantavirus strains (Bayou virus, Black Creek Canal virus, Cano Delgadito virus, Choclo virus, Laguna Negra virus, and Maporal virus). In the present study, we show that immunosuppression of hamsters followed by infection with a New World Hantavirus results in an acute disease that precisely mimics both Hantavirus disease in humans and Andes virus infection of hamsters. Conclusions/ Significance Infected hamsters showed specific clinical signs of disease and moreover, histological analysis of lung tissue showed signs of pulmonary edema and inflammation within alveolar septa. In this study, we were able to infect immunosuppressed hamsters with different New World Hantaviruses reaching a lethal outcome with signs of disease mimicking human disease.
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A lethal disease model for New World Hantaviruses using immunosuppressed Syrian hamsters.
Public Library of Science (PLoS), 2017Co-Authors: Valentijn Vergote, Lies Laenen, Bert Vanmechelen, Marc Van Ranst, Erik Verbeken, Piet MaesAbstract:Hantavirus, the hemorrhagic causative agent of two clinical diseases, is found worldwide with variation in severity, incidence and mortality. The most lethal Hantaviruses are found on the American continent where the most prevalent viruses like Andes virus and Sin Nombre virus are known to cause Hantavirus pulmonary syndrome. New World Hantavirus infection of immunocompetent hamsters results in an asymptomatic infection except for Andes virus and Maporal virus; the only Hantaviruses causing a lethal disease in immunocompetent Syrian hamsters mimicking Hantavirus pulmonary syndrome in humans.Hamsters, immunosuppressed with dexamethasone and cyclophosphamide, were infected intramuscularly with different New World Hantavirus strains (Bayou virus, Black Creek Canal virus, Caño Delgadito virus, Choclo virus, Laguna Negra virus, and Maporal virus). In the present study, we show that immunosuppression of hamsters followed by infection with a New World Hantavirus results in an acute disease that precisely mimics both Hantavirus disease in humans and Andes virus infection of hamsters.Infected hamsters showed specific clinical signs of disease and moreover, histological analysis of lung tissue showed signs of pulmonary edema and inflammation within alveolar septa. In this study, we were able to infect immunosuppressed hamsters with different New World Hantaviruses reaching a lethal outcome with signs of disease mimicking human disease
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hemorrhagic fever with renal syndrome in the new and Hantavirus pulmonary syndrome in the old world paradi se gm lost or regained
Virus Research, 2014Co-Authors: Jan Clement, Piet Maes, Marc Van RanstAbstract:Since the first clinical description in 1994 of the so-called “Hantavirus Pulmonary Syndrome” (HPS) as a “newly recognized disease”, Hantavirus infections have always been characterized as presenting in two distinct syndromes, the so-called “Hemorrhagic Fever with Renal Syndrome” (HFRS) in the Old World, with the kidney as main target organ, in contrast to HPS in the New World, with the lung as main target organ. However, European literature mentions already since 1934 a mostly milder local HFRS form, aptly named “nephropathia epidemica” (NE), and caused by the prototype European Hantavirus species Puumala virus (PUUV). Several NE reports dating from the 1980s and early 1990s described already non-cardiogenic HPS-like lung involvement, prior to any kidney involvement, and increasing evidence is now mounting that a considerable clinical overlap exists between HPS and HFRS. Moreover, growing immunologic insights point to common pathologic mechanisms, leading to capillary hyperpermeability, the cardinal feature of all Hantavirus infections, both of the New and Old World. It is now perhaps time to reconsider the paradigm of two “different” syndromes caused by viruses of the same Hantavirus genus in the same Bunyaviridae family, and to agree on a common, more logical disease denomination, such as simply and briefly “Hantavirus fever”.
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a proposal for new criteria for the classification of Hantaviruses based on s and m segment protein sequences
Infection Genetics and Evolution, 2009Co-Authors: Piet Maes, Detlev H Kruger, Jan Clement, Boris Klempa, Jelle Matthijnssens, Carleton D Gajdusek, Marc Van RanstAbstract:Hantaviruses, members of the family Bunyaviridae, are the causative agents of hemorrhagic fever with renal syndrome and Hantavirus cardiopulmonary syndrome. Hantaviruses are currently demarcated into species based on the guidelines provided by the International Committee on Taxonomy of Viruses (ICTV). These guidelines however, are often ignored by the descriptors of novel Hantaviruses. With this study we attempted to refine the second ICTV guideline for Hantavirus species demarcation by phylogenetically analyzing all in Genbank available complete sequences derived from the S, M or L segments of Hantaviruses. S and M segment amino acid sequence comparison allowed clear and unequivocal distinction between different Hantavirus species, and lead us to propose additional criteria for the demarcation of Hantavirus species (S segment amino acid distance >10% or M segment amino acid distance >12%) and Hantavirus groups (S segment amino distance >24% or M segment amino acid distance >32%). With this study, we propose to adjust the second rule of the ICTV classification guidelines ("a 7% difference in amino acid identity when comparing the complete S segment and M segment sequences") to a more appropriate rule, "a 10% difference in S segment similarity and a 12% difference in M segment similarity based on complete amino acid sequences" in accordance with the current situation in the Hantavirus field.
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Replication reduction neutralization test, a quantitative RT-PCR-based technique for the detection of neutralizing Hantavirus antibodies
Journal of virological methods, 2009Co-Authors: Piet Maes, Els Keyaerts, Véronique Nlandu-masunda, Jan Clement, Marc Van RanstAbstract:Hantaviruses, which are mainly rodent-borne viruses, cause hemorrhagic fever with renal syndrome in the Old World, and Hantavirus pulmonary syndrome in the New World. A neutralization test based on quantitative RT-PCR, the replication reduction neutralization test (RRNT), was developed for efficient detection of Hantavirus-neutralizing antibodies. The effectiveness of the RRNT was evaluated by examining several Hantaviruses and Hantavirus-specific convalescent human serum samples. All convalescent serum samples tested by RRNT caused significant decreases in Hantavirus genomes with only one specific Hantavirus species, which allowed a straightforward identification of the related Hantavirus. The results obtained by RRNT were completely comparable with the results obtained by focus reduction neutralization test (FRNT). The RRNT approach is a reliable and rapid alternative for FRNT, hitherto considered as the gold standard for Hantavirus serology.
Rainer G Ulrich - One of the best experts on this subject based on the ideXlab platform.
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replication in the mononuclear phagocyte system mps as a determinant of Hantavirus pathogenicity
Frontiers in Cellular and Infection Microbiology, 2020Co-Authors: Martin J Raftery, Pritesh Lalwani, Nina Lutteke, Lidija Kobak, Thomas Giese, Rainer G Ulrich, Lukas Radosa, D H Kruger, Gunther SchonrichAbstract:Members of different virus families including Hantaviridae cause viral hemorrhagic fevers (VHFs). The decisive determinants of Hantavirus-associated pathogenicity are still enigmatic. Pathogenic Hantavirus species, such as Puumala virus (PUUV), Hantaan virus (HTNV), Dobrava-Belgrade virus (DOBV), and Sin Nombre virus (SNV), are associated with significant case fatality rates. In contrast, Tula virus (TULV) only sporadically causes mild disease in immunocompetent humans and Prospect Hill virus (PHV) so far has not been associated with any symptoms. They are thus defined here as low pathogenic/apathogenic Hantavirus species. We found that productive infection of cells of the mononuclear phagocyte system (MPS), such as monocytes and dendritic cells (DCs), correlated well with the pathogenicity of Hantavirus species tested. HTNV (intermediate case fatality rates) replicated more efficiently than PUUV (low case fatality rates) in myeloid cells, whereas low pathogenic/apathogenic Hantavirus species did not produce any detectable virus titers. Analysis of PHPUV, a reassortant Hantavirus derived from a pathogenic (PUUV) and an apathogenic (PHV) Hantavirus species, indicated that the viral glycoproteins are not decisive for replication in MPS cells. Moreover, blocking acidification of endosomes with chloroquine decreased the number of TULV genomes in myeloid cells suggesting a post-entry block for low pathogenic/apathogenic Hantavirus species in myeloid cells. Intriguingly, pathogenic but not low pathogenic/apathogenic Hantavirus species induced conversion of monocytes into inflammatory DCs. The proinflammatory programming of MPS cells by pathogenic Hantavirus species required integrin signaling and viral replication. Our findings indicate that the capacity to replicate in MPS cells is a prominent feature of hantaviral pathogenicity.
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non human primates in outdoor enclosures risk for infection with rodent borne Hantaviruses
Veterinary Microbiology, 2011Co-Authors: Marc Mertens, Sandra Essbauer, Andreas Rang, J Schroder, Wolf D Splettstoesser, Christian Kretzschmar, Detlev H Kruger, Martin H Groschup, Kerstin Matzrensing, Rainer G UlrichAbstract:Different species of non-human primates have been exploited as animal disease models for human Hantavirus infections. To study the potential risk of natural Hantavirus infection of non-human primates, we investigated serum samples from non-human primates of three species living in outdoor enclosures of the German Primate Center (GPC), Gottingen, located in a Hantavirus endemic region of central Germany. For that purpose we used serological assays based on recombinant antigens of the bank vole (Myodes glareolus) transmitted Puumala virus (PUUV) and the common and field vole (Microtus arvalis, Microtus agrestis) associated Tula virus (TULV) which are both broadly geographically distributed in Germany. In 24 out of 251 (9.6%) monkey sera collected in 2006 PUUV- and/or TULV-reactive immunoglobulin G (IgG) antibodies were detected. Investigation of follow-up sera from 13 animals confirmed for two animals a seroconversion due to Hantavirus exposure at the GPC. To prove the origin of the infection, wild rodents from the surrounding regions were analyzed by Hantavirus-specific reverse transcriptase-PCR analysis. In 6 of the 73 investigated bank voles and 3 of the 19 investigated Microtus spp. PUUV- and TULV-specific nucleic acid sequences, respectively, were detected. In conclusion, our investigations demonstrate for the first time natural infections of non-human primates in outdoor enclosures in Germany. These findings highlight the importance of Hantavirus surveillance in those primate housings and corresponding preventive measures against wild rodents, particularly in Hantavirus endemic regions.
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Non-human primates in outdoor enclosures: Risk for infection with rodent-borne Hantaviruses
Veterinary Microbiology, 2010Co-Authors: Marc Mertens, Sandra Essbauer, Andreas Rang, J Schroder, Wolf D Splettstoesser, Christian Kretzschmar, Martin H Groschup, D H Kruger, K. Mätz-rensing, Rainer G UlrichAbstract:Different species of non-human primates have been exploited as animal disease models for human Hantavirus infections. To study the potential risk of natural Hantavirus infection of non-human primates, we investigated serum samples from non-human primates of three non-human primate species living in outdoor enclosures of the German Primate Center (GPC), Göttingen, located in a Hantavirus endemic region of central Germany. For that purpose we used serological assays based on recombinant antigens of the bank vole () transmitted (PUUV) and the common and field vole (, ) associated (TULV) which are both broadly geographically distributed in Germany. In 24 out of 251 (9.6%) monkey sera collected in 2006 PUUV- and/or TULV-reactive immunoglobulin G (IgG) antibodies were detected. Investigation of follow-up sera from 13 animals confirmed for two animals a seroconversion due to Hantavirus exposure at the GPC. To prove the origin of the infection, wild rodents from the surrounding regions were analyzed by Hantavirus-specific reverse transcriptase PCR analysis. In 6 of the 73 investigated bank voles and 3 of the 19 investigated spp. PUUV- and TULV-specific nucleic acid sequences, respectively, were detected. In conclusion, our investigations demonstrate for the first time natural infections of non-human primates in outdoor enclosures in Germany. These findings highlight the importance of Hantavirus surveillance in those primate housings and corresponding preventive measures against wild rodents, particularly in Hantavirus endemic regions.
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Hantavirus species in India: a retrospective study.
Indian journal of medical microbiology, 2009Co-Authors: Sunil Chandy, Rainer G Ulrich, Kumiko Yoshimatsu, Jiro Arikawa, Priya Abraham, Megumi Okumura, George John, Gopalan SridharanAbstract:Hantaviruses cause hemorrhagic fever with renal syndrome in Europe and Asia. There are about 20 documented Hantavirus species and newer species are being described worldwide, especially in non-rodent reservoirs, i.e shrews. Focus reduction neutralization test is the classical serotyping technique for Hantavirus. However, this study employs a previously established serotyping ELISA, to retrospectively analyze known Hantavirus IgG reactive samples for infecting serotypes. The result suggests presence of Thailand virus- like and Hantaan virus -like strains in India.
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Hantavirus-induced immunity in rodent reservoirs and humans
Immunological Reviews, 2008Co-Authors: Gunther Schonrich, Andreas Rang, Martin J Raftery, Nina Lutteke, Nathalie Charbonnel, Rainer G UlrichAbstract:Hantaviruses are predominantly rodent-borne pathogens, although recently novel shrew-associated Hantaviruses were found. Within natural reservoir hosts, hantairuses do not cause obvious pathogenetic effects; transmission to humans, however, can lead to hemorrhagic fever with renal syndrome or Hantavirus cardiopulmonary syndrome, depending on the virus species involved. This review is focussed on the recent knowledge on Hantavirus-induced immune responses in rodent reservoirs and humans and their impact on susceptibility, transmission, and outcome of Hantavirus infections. In addition, this review incorporates a discussion on the potential role of direct cell-virus interactions in the pathogenesis of Hantavirus infections in humans. Finally, questions for further research efforts on the immune responses in potential Hantavirus reservoir hosts and humans are summarized.
Piet Maes - One of the best experts on this subject based on the ideXlab platform.
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A lethal disease model for New World Hantaviruses using immunosuppressed Syrian hamsters.
PLoS neglected tropical diseases, 2017Co-Authors: Valentijn Vergote, Lies Laenen, Bert Vanmechelen, Marc Van Ranst, Erik Verbeken, Piet MaesAbstract:Background Hantavirus, the hemorrhagic causative agent of two clinical diseases, is found worldwide with variation in severity, incidence and mortality. The most lethal Hantaviruses are found on the American continent where the most prevalent viruses like Andes virus and Sin Nombre virus are known to cause Hantavirus pulmonary syndrome. New World Hantavirus infection of immunocompetent hamsters results in an asymptomatic infection except for Andes virus and Maporal virus; the only Hantaviruses causing a lethal disease in immunocompetent Syrian hamsters mimicking Hantavirus pulmonary syndrome in humans. Methodology/Principal findings Hamsters, immunosuppressed with dexamethasone and cyclophosphamide, were infected intramuscularly with different New World Hantavirus strains (Bayou virus, Black Creek Canal virus, Cano Delgadito virus, Choclo virus, Laguna Negra virus, and Maporal virus). In the present study, we show that immunosuppression of hamsters followed by infection with a New World Hantavirus results in an acute disease that precisely mimics both Hantavirus disease in humans and Andes virus infection of hamsters. Conclusions/ Significance Infected hamsters showed specific clinical signs of disease and moreover, histological analysis of lung tissue showed signs of pulmonary edema and inflammation within alveolar septa. In this study, we were able to infect immunosuppressed hamsters with different New World Hantaviruses reaching a lethal outcome with signs of disease mimicking human disease.
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A lethal disease model for New World Hantaviruses using immunosuppressed Syrian hamsters.
Public Library of Science (PLoS), 2017Co-Authors: Valentijn Vergote, Lies Laenen, Bert Vanmechelen, Marc Van Ranst, Erik Verbeken, Piet MaesAbstract:Hantavirus, the hemorrhagic causative agent of two clinical diseases, is found worldwide with variation in severity, incidence and mortality. The most lethal Hantaviruses are found on the American continent where the most prevalent viruses like Andes virus and Sin Nombre virus are known to cause Hantavirus pulmonary syndrome. New World Hantavirus infection of immunocompetent hamsters results in an asymptomatic infection except for Andes virus and Maporal virus; the only Hantaviruses causing a lethal disease in immunocompetent Syrian hamsters mimicking Hantavirus pulmonary syndrome in humans.Hamsters, immunosuppressed with dexamethasone and cyclophosphamide, were infected intramuscularly with different New World Hantavirus strains (Bayou virus, Black Creek Canal virus, Caño Delgadito virus, Choclo virus, Laguna Negra virus, and Maporal virus). In the present study, we show that immunosuppression of hamsters followed by infection with a New World Hantavirus results in an acute disease that precisely mimics both Hantavirus disease in humans and Andes virus infection of hamsters.Infected hamsters showed specific clinical signs of disease and moreover, histological analysis of lung tissue showed signs of pulmonary edema and inflammation within alveolar septa. In this study, we were able to infect immunosuppressed hamsters with different New World Hantaviruses reaching a lethal outcome with signs of disease mimicking human disease
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hemorrhagic fever with renal syndrome in the new and Hantavirus pulmonary syndrome in the old world paradi se gm lost or regained
Virus Research, 2014Co-Authors: Jan Clement, Piet Maes, Marc Van RanstAbstract:Since the first clinical description in 1994 of the so-called “Hantavirus Pulmonary Syndrome” (HPS) as a “newly recognized disease”, Hantavirus infections have always been characterized as presenting in two distinct syndromes, the so-called “Hemorrhagic Fever with Renal Syndrome” (HFRS) in the Old World, with the kidney as main target organ, in contrast to HPS in the New World, with the lung as main target organ. However, European literature mentions already since 1934 a mostly milder local HFRS form, aptly named “nephropathia epidemica” (NE), and caused by the prototype European Hantavirus species Puumala virus (PUUV). Several NE reports dating from the 1980s and early 1990s described already non-cardiogenic HPS-like lung involvement, prior to any kidney involvement, and increasing evidence is now mounting that a considerable clinical overlap exists between HPS and HFRS. Moreover, growing immunologic insights point to common pathologic mechanisms, leading to capillary hyperpermeability, the cardinal feature of all Hantavirus infections, both of the New and Old World. It is now perhaps time to reconsider the paradigm of two “different” syndromes caused by viruses of the same Hantavirus genus in the same Bunyaviridae family, and to agree on a common, more logical disease denomination, such as simply and briefly “Hantavirus fever”.
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a proposal for new criteria for the classification of Hantaviruses based on s and m segment protein sequences
Infection Genetics and Evolution, 2009Co-Authors: Piet Maes, Detlev H Kruger, Jan Clement, Boris Klempa, Jelle Matthijnssens, Carleton D Gajdusek, Marc Van RanstAbstract:Hantaviruses, members of the family Bunyaviridae, are the causative agents of hemorrhagic fever with renal syndrome and Hantavirus cardiopulmonary syndrome. Hantaviruses are currently demarcated into species based on the guidelines provided by the International Committee on Taxonomy of Viruses (ICTV). These guidelines however, are often ignored by the descriptors of novel Hantaviruses. With this study we attempted to refine the second ICTV guideline for Hantavirus species demarcation by phylogenetically analyzing all in Genbank available complete sequences derived from the S, M or L segments of Hantaviruses. S and M segment amino acid sequence comparison allowed clear and unequivocal distinction between different Hantavirus species, and lead us to propose additional criteria for the demarcation of Hantavirus species (S segment amino acid distance >10% or M segment amino acid distance >12%) and Hantavirus groups (S segment amino distance >24% or M segment amino acid distance >32%). With this study, we propose to adjust the second rule of the ICTV classification guidelines ("a 7% difference in amino acid identity when comparing the complete S segment and M segment sequences") to a more appropriate rule, "a 10% difference in S segment similarity and a 12% difference in M segment similarity based on complete amino acid sequences" in accordance with the current situation in the Hantavirus field.
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Replication reduction neutralization test, a quantitative RT-PCR-based technique for the detection of neutralizing Hantavirus antibodies
Journal of virological methods, 2009Co-Authors: Piet Maes, Els Keyaerts, Véronique Nlandu-masunda, Jan Clement, Marc Van RanstAbstract:Hantaviruses, which are mainly rodent-borne viruses, cause hemorrhagic fever with renal syndrome in the Old World, and Hantavirus pulmonary syndrome in the New World. A neutralization test based on quantitative RT-PCR, the replication reduction neutralization test (RRNT), was developed for efficient detection of Hantavirus-neutralizing antibodies. The effectiveness of the RRNT was evaluated by examining several Hantaviruses and Hantavirus-specific convalescent human serum samples. All convalescent serum samples tested by RRNT caused significant decreases in Hantavirus genomes with only one specific Hantavirus species, which allowed a straightforward identification of the related Hantavirus. The results obtained by RRNT were completely comparable with the results obtained by focus reduction neutralization test (FRNT). The RRNT approach is a reliable and rapid alternative for FRNT, hitherto considered as the gold standard for Hantavirus serology.
Sergey P Morzunov - One of the best experts on this subject based on the ideXlab platform.
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Hantavirus infection suppresses thrombospondin-1 expression in cultured endothelial cells in a strain-specific manner
Frontiers Media S.A., 2016Co-Authors: Sergey P Morzunov, Albert Anatolevich Rizvanov, Svetlana F. Khaiboullina, Stephen St. Jeor, Vincent C. LombardiAbstract:Hantavirus infection is associated with two frequently fatal diseases in humans: hemorrhagic fever with renal syndrome (HFRS) and Hantavirus pulmonary syndrome (HPS). The pathogenesis of Hantavirus infection is complex and not fully understood; however, it is believed to involve virus-induced hyperinflammatory immune responses. Thrombospondin-1 (THBS1) is a large homotrimeric protein that plays a putative role in regulating blood homeostasis. Hyperresponsiveness to inflammatory stimuli has also been associated with defects in the THBS1 gene. Our data suggest that Hantavirus infection of human umbilical cord vein endothelial cells (HUVEC) suppress the accumulation of THBS1 in the extracellular matrix. Additionally, this suppression is dependent on virus replication, implying a direct mechanism of action. Our data also imply that the pathogenic Andes and Hantaan strains inhibit THBS1 expression while the non-pathogenic Prospect Hill strain showed little inhibition. These observations suggest that a dysregulation of THBS1 may contribute to the pathogenesis of Hantavirus infection
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Hantaviral Proteins: Structure, Functions, and Role in Hantavirus Infection.
Frontiers in microbiology, 2015Co-Authors: M. Muyangwa, Sergey P Morzunov, Ekaterina V. Martynova, Svetlana F. Khaiboullina, Albert Anatolevich RizvanovAbstract:Hantaviruses are the members of the family Bunyaviridae that are naturally maintained in the populations of small mammals, mostly rodents. Most of these viruses can easily infect humans through contact with aerosols or dust generated by contaminated animal waste products. Depending on the particular Hantavirus involved, human infection could result in either Hemorrhagic Fever with Renal Syndrome (HFRS) or in Hantavirus Cardiopulmonary Syndrome (HCPS). In the past few years, clinical cases of the Hantavirus caused diseases have been on the rise. Understanding structure of the Hantavirus genome and the functions of the key viral proteins is critical for the therapeutic agents’ research. This paper gives a brief overview of the current knowledge on the structure and properties of the Hantavirus nucleoprotein and the glycoproteins.
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Prevalence of Hantavirus in Four Species of Neotoma from Arizona and Utah
Journal of Mammalogy, 1998Co-Authors: M. Denise Dearing, Sergey P Morzunov, Antonio M. Mangione, William H. Karasov, Elmer W. Otteson, Stephen St. JeorAbstract:Sin Nombre virus (SNV), a Hantavirus, can cause severe respiratory illness and death in humans. The primary carrier is the deer mouse, Peromyscus maniculatus , but other species of rodents may be infected with the virus. We screened four species of woodrats ( Neotoma ) for Hantavirus using both enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) tests. Woodrats were collected from a variety of habitats in Arizona and Utah. Only Neotoma lepida tested serologically positive in an ELISA assay for Hantavirus and also contained SNV RNA. Moreover, across three distinct populations of N. lepida , individuals that tested serum positive were restricted to one population near Jericho, Utah. The prevalence of Hantavirus in this population was 27% (4 of 15).
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genetic diversity and epidemiology of Hantaviruses in argentina
The Journal of Infectious Diseases, 1998Co-Authors: Silvana Levis, Sergey P Morzunov, Joan E Rowe, Delia Enria, Noemi Pini, Gladys E Calderon, Martha Sabattini, Stephen St. JeorAbstract:: Phylogenetic analysis of a 292-nucleotide (nt) fragment of the Hantavirus M genome segment from 36 rodent and 13 human samples from three known foci of Hantavirus infection in Argentina was conducted. A 1654-nt fragment of the M genome segment was analyzed for 1 representative of 7 genetically distinct Hantavirus lineages identified. Additionally, the nt sequence of the complete M genome segments of Lechiguanas, Oran, and Hu39694 Hantavirus genotypes was determined. nt sequence comparisons reveal that 7 Hantavirus lineages from Argentina differ from each other by 11.5%-21.8% and from Sin Nombre, Bayou, and Black Creek Canal viruses by 23.8%-26.5%. Phylogenetic analyses demonstrate that they form a unique, separate branch within the clade containing other New World sigmodontine-borne Hantaviruses. Most Oligoryzomys-borne Hantavirus genotypes clearly map together. The Oligoryzomys-borne genotypes Lechiguanas, Oran, and Andes appear to be associated with human disease. Oligoryzomys longicaudatus was identified as the likely rodent reservoir for Andes virus.
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isolation of black creek canal virus a new Hantavirus from sigmodon hispidus in florida
Journal of Medical Virology, 1995Co-Authors: Pierre E Rollin, Eugene V Ravkov, Sergey P Morzunov, Walter Livingstone, Marty Monroe, Thomas G. G Ksiazek, Mary Lane Martin, Gregory E. Glass, Luanne H. Elliott, Ali S. KhanAbstract:Numerous rodents were trapped for serologic and virologic studies following the identification of a Hantavirus pulmonary syndrome (HPS) case in Dade County, Florida. Cotton rats (Sigmodon hispidus) were the most frequently captured rodent and displayed the highest seroprevalence to a variety of Hantavirus antigens. Hantavirus genome RNA was detected in all the seropositive cotton rats tested, using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay. A virus was isolated from tissues of two seropositive cotton rats by cultivation of lung and spleen homogenates on Vero E6 cells. Nucleotide sequence information obtained by direct RT-PCR and the serologic relationships of this virus with the other Hantaviruses indicate that this virus, Black Creek Canal virus, represents a new Hantavirus distinct from the previously known serotypes. © 1995 Wiley-Liss, Inc.
Pierre E Rollin - One of the best experts on this subject based on the ideXlab platform.
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twenty year summary of surveillance for human Hantavirus infections united states
Emerging Infectious Diseases, 2013Co-Authors: Barbara Knust, Pierre E RollinAbstract:In the past 20 years of surveillance for Hantavirus in humans in the United States, 624 cases of Hantavirus pulmonary syndrome (HPS) have been reported, 96% of which occurred in states west of the Mississippi River. Most Hantavirus infections are caused by Sin Nombre virus, but cases of HPS caused by Bayou, Black Creek Canal, Monongahela, and New York viruses have been reported, and cases of domestically acquired hemorrhagic fever and renal syndrome caused by Seoul virus have also occurred. Rarely, Hantavirus infections result in mild illness that does not progress to HPS. Continued testing and surveillance of clinical cases in humans will improve our understanding of the etiologic agents involved and the spectrum of diseases.
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Hantavirus transmission in the United States.
Emerging infectious diseases, 1997Co-Authors: Rachel Wells, Thomas G. G Ksiazek, Ali S. Khan, Pierre E Rollin, Joni Young, R. Joel Williams, Lori R. Armstrong, Kristi Busico, Sherif R. Zaki, Stuart T. NicholAbstract:In 1996, investigation of a Hantavirus pulmonary syndrome (HPS) outbreak in southern Argentina found evidence of person-to-person transmission of a Hantavirus. The infection control ramifications of this finding led to this review of Hantavirus epidemiology in the United States; the review suggests that Sin Nombre virus infection is rarely, if ever, transmitted from person to person and that existing guidelines for prevention of HPS remain appropriate for North America.
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isolation of black creek canal virus a new Hantavirus from sigmodon hispidus in florida
Journal of Medical Virology, 1995Co-Authors: Pierre E Rollin, Eugene V Ravkov, Sergey P Morzunov, Walter Livingstone, Marty Monroe, Thomas G. G Ksiazek, Mary Lane Martin, Gregory E. Glass, Luanne H. Elliott, Ali S. KhanAbstract:Numerous rodents were trapped for serologic and virologic studies following the identification of a Hantavirus pulmonary syndrome (HPS) case in Dade County, Florida. Cotton rats (Sigmodon hispidus) were the most frequently captured rodent and displayed the highest seroprevalence to a variety of Hantavirus antigens. Hantavirus genome RNA was detected in all the seropositive cotton rats tested, using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay. A virus was isolated from tissues of two seropositive cotton rats by cultivation of lung and spleen homogenates on Vero E6 cells. Nucleotide sequence information obtained by direct RT-PCR and the serologic relationships of this virus with the other Hantaviruses indicate that this virus, Black Creek Canal virus, represents a new Hantavirus distinct from the previously known serotypes. © 1995 Wiley-Liss, Inc.
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serologic and genetic identification of peromyscus maniculatus as the primary rodent reservoir for a new Hantavirus in the southwestern united states
The Journal of Infectious Diseases, 1994Co-Authors: James E Childs, Sergey P Morzunov, Thomas G. G Ksiazek, Pierre E Rollin, Christina F Spiropoulou, John W Krebs, Gary O Maupin, Kenneth L Gage, John Sarisky, Russell E EnscoreAbstract:An outbreak of Hantavirus pulmonary syndrome (HPS) in the southwestern United States was etiologically linked to a newly recognized Hantavirus. Knowledge that Hantaviruses are maintained in rodent reservoirs stimulated a field and laboratory investigation of 1696 small mammals of 31 species. The most commonly captured rodent, the deer mouse (Peromyscus maniiculatus), had the highest antibody prevalence (30%) to four Hantavirus antigens. Antibody also was detected in 10 other species of rodent and in 1 species of rabbit. Reverse transcriptase-polymerase chain reaction (RT-PCR) products of Hantavirus from rodent tissues were indistinguishable from those from human HPS patients
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serologic and genetic identification of peromyscus maniculatus as the primary rodent reservoir for a new Hantavirus in the southwestern united states
The Journal of Infectious Diseases, 1994Co-Authors: James E Childs, Sergey P Morzunov, Thomas G. G Ksiazek, Pierre E Rollin, Christina F Spiropoulou, John W Krebs, Gary O Maupin, Kenneth L Gage, John Sarisky, Russell E EnscoreAbstract:: An outbreak of Hantavirus pulmonary syndrome (HPS) in the southwestern United States was etiologically linked to a newly recognized Hantavirus. Knowledge that Hantaviruses are maintained in rodent reservoirs stimulated a field and laboratory investigation of 1696 small mammals of 31 species. The most commonly captured rodent, the deer mouse (Peromyscus maniculatus), had the highest antibody prevalence (30%) to four Hantavirus antigens. Antibody also was detected in 10 other species of rodent and in 1 species of rabbit. Reverse transcriptase-polymerase chain reaction (RT-PCR) products of Hantavirus from rodent tissues were indistinguishable from those from human HPS patients. More than 96% of the seropositive P. maniculatus were positive by RT-PCR, suggesting chronic infection. Antibody prevalences were similar among P. maniculatus trapped from Arizona (33%), New Mexico (29%), and Colorado (29%). The numeric dominance of P. maniculatus, the high prevalence of antibody, and the RT-PCR findings implicate this species as the primary rodent reservoir for a new Hantavirus in the southwestern United States.
