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Jong-yil Chai - One of the best experts on this subject based on the ideXlab platform.
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Infection status with zoonotic trematode metacercariae in fish from Lao PDR
Acta Tropica, 2019Co-Authors: Jong-yil Chai, Woon-mok Sohn, Bounlay PhommasackAbstract:Abstract Studies on the infection status with fishborne zoonotic trematode (FZT) metacercariae were reviewed with our data, which obtained from the fish survey in 8 administrative localities, i.e., Vientiane Municipality, Luang Prabang, Xieng Khouang, Vientiane, Khammuane, Savannakhet, Saravane and Champasak Provinces of Lao PDR (Laos) in 10 times. Total 1,046 freshwater fishes (69 species) were examined with the artificial digestion method from 2002 to 2011. Total 6 species of zoonotic trematode, i.e., Opisthorchis viverrini, Haplorchis taichui, Haplorchis yokogawai, Haplorchis pumilio, Centrocestus formosanus and Procerovum varium, metacercariae were detected in fishes from Laos. The metacercariae of O. viverrini were detected in 284 (37.9%) out of 749 fishes (26 spp.), and their average density was 297 per fish infected. The prevalences of 4 heterophyid species, i.e., H. taichui, H. yokogawai, H. pumilio and C. formosanus, metacercariae were 45.4% (out of 941 fishes in 46 spp. examined), 45.1% (811 fishes in 35 spp.), 8.8% (457 fishes in 11 spp.) and 18.1% (475 fishes in 13 spp.), their average densities were 291, 218, 4 and 15 per fish infected respectively. The endemicities of O. viverrini metacercariae were much higher in fishes from Savannakhet Province and Vientiane Municipality (2002). Those of H. taichui metacercariae were much higher in fishes from Khammuane and Luang Prabang (2011) Provinces. By the present study, it was confirmed that some species of FZT metacercariae are highly prevalent in fishes from Laos.
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Infection Status of Zoonotic Trematode Metacercariae in Fishes from Vientiane Municipality and Champasak Province in Lao PDR.
Korean Journal of Parasitology, 2015Co-Authors: Han Sol Park, Jong-yil Chai, Woon-mok Sohn, Tai-soon Yong, Bounnaloth Insisiengmay, Bounlay PhommasackAbstract:The infection status of fishborne zoonotic trematode (FZT) metacercariae was investigated in fishes from 2 localities of Lao PDR. Total 157 freshwater fishes (17 species) were collected in local markets of Vientiane Municipality and Champasak Province in December 2010 and July 2011, and each fish was examined by the artificial digestion method. Total 6 species of FZT metacercariae, i.e., Opisthorchis viverrini, Haplorchis taichui, Haplorchis yokogawai, Haplorchis pumilio, Centrocestus formosanus, and Procerovum varium, were detected in fishes from Vientiane Municipality. The metacercariae of O. viverrini were detected in 50 (49.5%) out of 101 fishes (6 species), and their average number was 154 per fish infected. The remaining 5 species of heterophyid metacercariae were detected in 36.8%, 65.8%, 9.4%, 23.9%, and 5.1% fishes examined, and their average densities were 12, 1,038, 4, 15, and 13 per infected fish, respectively. In fishes from Champasak Province, 3 species of FZT metacercariae, i.e., O. viverrini, H. taichui, and H. yokogawai, were detected. Only 2 O. viverrini metacercariae were found in only 1 Barbonymus schwanefeldi. The metacercariae of H. taichui and H. yokogawai were detected in 60.0% and 50.0% of fishes examined, and their average densities were 47 and 28 per fish infected. By the present study, it has been confirmed that several species of FZT metacercariae are prevalent in fishes from Vientiane Municipality, with P. varium being a new member of FZT in Lao PDR. In comparison, FZT metacercariae are less prevalent in fishes from Champasak Province.
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prevalence of Haplorchis taichui among humans and fish in luang prabang province lao pdr
Acta Tropica, 2014Co-Authors: Woon-mok Sohn, Tai-soon Yong, Bounlay Phommasack, Bounnaloth Insisiengmay, Bong Kwang Jung, Virasack Banouvong, Jong-yil ChaiAbstract:Abstract This study confirmed the prevalence of the intestinal fluke Haplorchis taichui (Trematoda: Heterophyidae) among people and fish in Luang Prabang Province, Lao PDR. Fecal specimens were collected from 559 riparian people (229 males and 330 females), residing in 4 Districts (Luang Prabang, Xieng Ngeun, Pak Ou, and Nam Bak) and were examined by the Kato-Katz fecal smear technique. The overall helminth egg positive rate was 64.9%. The positive rate for small trematode eggs (STE), which may include H. taichui and other heterophyids, Opisthorchis viverrini, and lecithodendriids, was 15.2%. For recovery of adult helminths, 10 STE-positive people were treated with 40 mg/kg praziquantel and 15 mg/kg pyrantel pamoate, and then purged. Mixed infections with 3 Haplorchis species (H. taichui, H. pumilio, and H. yokogawai), a species of cestode (Taenia saginata), and several species of nematodes including Enterobius vermicularis and hookworms were found. The worm load for trematodes was exclusively high for H. taichui with an average of 7691 specimens per infected person, followed by H. yokogawai (8.3 specimens) and H. pumilio (4.1 specimens). Out of 207 freshwater fish (17 species) purchased in a market in Luang Prabang District, 138 (67%) harboured H. taichui metacercariae (metacercarial burden per fish; 520). Lower prevalence of fish and lower metacercarial density were observed for H. yokogawai (52% and 50 per fish, respectively) and H. pumilio (18% and 3 per fish, respectively). STE found in the surveyed population of Luang Prabang Province were verified to be those of intestinal fukes, particularly H. taichui.
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Zoonotic Trematode Metacercariae in Fish from Phnom Penh and Pursat, Cambodia
Korean Journal of Parasitology, 2014Co-Authors: Jong-yil Chai, Woon-mok Sohn, Tai-soon Yong, Eui Hyug Hoang, Byoung Kuk Na, Cheong Ha Yoon, Hoo Gn Jeoung, Duong SocheatAbstract:A survey was performed to investigate the infection status of freshwater fish with zoonotic trematode metacercariae in Phnom Penh and Pursat Province, Cambodia. All collected fish with ice were transferred to our laboratory and examined using the artificial digestion method. In fish from Phnom Penh, 2 kinds of metacercariae (Opisthorchis viverrini and Haplorchis yokogawai) were detected. O. viverrini metacercariae were positive in 37 (50.0%) of 74 fish in 11 species (average no. metacercariae/fish, 18.6). H. yokogawai metacercariae were detected in 23 (57.5%) of 40 fish in 5 species (average no. metacercariae/fish, 21.0). In fish from Pursat Province, 5 kinds of metacercariae (O. viverrini, H. yokogawai, Haplorchis pumilio, Centrocestus formosanus, and Procerovum sp.) were detected; O. viverrini metacercariae (n=3) in 2 fish species (Henicorhynchus lineatus and Puntioplites falcifer), H. yokogawai metacercariae (n=51) in 1 species (P. falcifer), H. pumilio metacercariae (n=476) in 2 species (H. lineatus and Pristolepis fasciata), C. formosanus metacercariae (n=1) in 1 species (H. lineatus), and Procerovum sp. metacercariae (n=63) in 1 species (Anabas testudineus). From the above results, it has been confirmed that various freshwater fish play the role of a second intermediate host for zoonotic trematodes (O. viverrini, H. yokogawai, H. pumilio, C. formosanus, and Procerovum sp.) in Cambodia.
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complete mitochondrial genome of Haplorchis taichui and comparative analysis with other trematodes
Korean Journal of Parasitology, 2013Co-Authors: Seongjun Choe, Jong-yil Chai, Woon-mok Sohn, Han Sol Park, Hyeongkyu Jeon, Tai-soon YongAbstract:Mitochondrial genomes have been extensively studied for phylogenetic purposes and to investigate intra- and interspecific genetic variations. In recent years, numerous groups have undertaken sequencing of platyhelminth mitochondrial genomes. Haplorchis taichui (family Heterophyidae) is a trematode that infects humans and animals mainly in Asia, including the Mekong River basin. We sequenced and determined the organization of the complete mitochondrial genome of H. taichui. The mitochondrial genome is 15,130 bp long, containing 12 protein-coding genes, 2 ribosomal RNAs (rRNAs, a small and a large subunit), and 22 transfer RNAs (tRNAs). Like other trematodes, it does not encode the atp8 gene. All genes are transcribed from the same strand. The ATG initiation codon is used for 9 protein-coding genes, and GTG for the remaining 3 (nad1, nad4, and nad5). The mitochondrial genome of H. taichui has a single long non-coding region between trnE and trnG. H. taichui has evolved as being more closely related to Opisthorchiidae than other trematode groups with maximal support in the phylogenetic analysis. Our results could provide a resource for the comparative mitochondrial genome analysis of trematodes, and may yield genetic markers for molecular epidemiological investigations into intestinal flukes.
David Blair - One of the best experts on this subject based on the ideXlab platform.
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the ribosomal transcription units of Haplorchis pumilio and h taichui and the use of 28s rdna sequences for phylogenetic identification of common heterophyids in vietnam
Parasites & Vectors, 2017Co-Authors: Thanh Hoa Le, Khue Thi Nguyen, Nga Thi Bich Nguyen, Huong Thi Thanh Doan, Do Trung Dung, David BlairAbstract:Background Heterophyidiasis is now a major public health threat in many tropical countries. Species in the trematode family Heterophyidae infecting humans include Centrocestus formosanus, Haplorchis pumilio, H. taichui, H. yokogawai, Procerovum varium and Stellantchasmus falcatus. For molecular phylogenetic and systematic studies on trematodes, we need more prospective markers for taxonomic identification and classification. This study provides near-complete ribosomal transcription units (rTU) from Haplorchis pumilio and H. taichui and demonstrates the use of 28S rDNA sequences for identification and phylogenetic analysis.
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The ribosomal transcription units of Haplorchis pumilio and H. taichui and the use of 28S rDNA sequences for phylogenetic identification of common heterophyids in Vietnam
Parasites & Vectors, 2017Co-Authors: Thanh Hoa Le, Khue Thi Nguyen, Nga Thi Bich Nguyen, Huong Thi Thanh Doan, Do Trung Dung, David BlairAbstract:Background Heterophyidiasis is now a major public health threat in many tropical countries. Species in the trematode family Heterophyidae infecting humans include Centrocestus formosanus , Haplorchis pumilio , H. taichui , H. yokogawai , Procerovum varium and Stellantchasmus falcatus. For molecular phylogenetic and systematic studies on trematodes, we need more prospective markers for taxonomic identification and classification. This study provides near-complete ribosomal transcription units (rTU) from Haplorchis pumilio and H. taichui and demonstrates the use of 28S rDNA sequences for identification and phylogenetic analysis. Results The near-complete ribosomal transcription units (rTU), consisting of 18S, ITS1, 5.8S, ITS2 and 28S rRNA genes and spacers, from H. pumilio and H. taichui from human hosts in Vietnam, were determined and annotated. Sequence analysis revealed tandem repetitive elements in ITS1 in H. pumilio and in ITS2 in H. taichui. A phylogenetic tree inferred from 28S rDNA sequences of 40 trematode strains/species, including 14 Vietnamese heterophyid individuals, clearly confirmed the status of each of the Vietnamese species: Centrocestus formosanus, Haplorchis pumilio , H. taichui , H. yokogawai , Procerovum varium and Stellantchasmus falcatus . However, the family Heterophyidae was clearly not monophyletic, with some genera apparently allied with other families within the superfamily Opisthorchioidea (i.e. Cryptogonimidae and Opisthorchiidae). These families and their constituent genera require substantial re-evaluation using a combination of morphological and molecular data. Our new molecular data will assist in such studies. Conclusions The 28S rDNA sequences are conserved among individuals within a species but varied between genera. Based on analysis of 40 28S rDNA sequences representing 19 species in the superfamily Opisthorchioidea and an outgroup taxon ( Alaria alata , family Diplostomidae), six common human pathogenic heterophyids were identified and clearly resolved. The phylogenetic tree inferred from these sequences again confirmed anomalies in molecular placement of some members of the family Heterophyidae and demonstrates the need for reappraisal of the entire superfamily Opisthorchioidea. The new sequences provided here supplement those already available in public databases and add to the array of molecular tools that can be used for the diagnosis of heterophyid species in human and animal infections.
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Haplorchis pumilio and h taichui in vietnam discriminated using its 2 dna sequence data from adults and larvae
Experimental Parasitology, 2009Co-Authors: Anders Dalsgaard, David Blair, Thanh Hoa LeAbstract:Samples of Haplorchis taichui and Haplorchis pumilio of different life-stages (cercariae, metacercariae and adults) and from different host species (snail, fish, dog, cat and human) were collected in Nghe An and Nam Dinh Provinces in Vietnam. Samples from Thailand were available for comparison. All adults and metacercariae were initially identified using morphological criteria. Polymerase chain reaction (PCR) assays were developed for discriminating between the species. The complete sequence for the nuclear ribosomal internal transcribed spacer-2 (ITS-2) was obtained from one adult and one metacercaria of H. taichui and three adults and three metacercariae of H. pumilio from Vietnam. Sequences from cercariae from three different snails clustered with those of H. pumilio. Intra-individual variation in the ITS-2 region was detected by sequencing of cloned PCR products. These are the first sequences from Vietnamese Haplorchis spp. to be reported and demonstrate that H. taichui and H. pumilio can be identified unambiguously from any life-stage, including the cercarial stage that is difficult to identify using morphology. Discrepancies in the literature are discussed and examples of apparent misidentification highlighted. The data provide a resource to assist in taxonomic studies on heterophyids, in the design of probes for diagnosis and for field surveys to identify infection in snails.
Jitra Waikagul - One of the best experts on this subject based on the ideXlab platform.
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genetic differences among Haplorchis taichui populations in indochina revealed by mitochondrial cox1 sequences
Journal of Helminthology, 2017Co-Authors: Urusa Thaenkham, Surapol Sanguankiat, Do Trung Dung, Paron Dekumyoy, Supaporn Nuamtanong, Orawan Phuphisut, Tippayarat Yoonuan, Y Vonghachack, V Y Belizario, Jitra WaikagulAbstract:Haplorchis taichui is an intestinal heterophyid fluke that is pathogenic to humans. It is widely distributed in Asia, with a particularly high prevalence in Indochina. Previous work revealed that the lack of gene flow between three distinct populations of Vietnamese H. taichui can be attributed to their geographic isolation with no interconnected river basins. To test the hypothesis that interconnected river basins allow gene flow between otherwise isolated populations of H. taichui, as previously demonstrated for another trematode, Opisthorchis viverrini, we compared the genetic structures of seven populations of H. taichui from various localities in the lower Mekong Basin, in Thailand and Laos, with those in Vietnam, using the mitochondrial cytochrome c oxidase subunit 1 (COX1) gene. To determine the gene flow between these H. taichui populations, we calculated their phylogenetic relationships, genetic distances and haplotype diversity. Each population showed very low nucleotide diversity at this locus. However, high levels of genetic differentiation between the populations indicated very little gene flow. A phylogenetic analysis divided the populations into four clusters that correlated with the country of origin. The negligible gene flow between the Thai and Laos populations, despite sharing the Mekong Basin, caused us to reject our hypothesis. Our data suggest that the distribution of H. taichui populations was incidentally associated with national borders.
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genetic differences among vietnamese Haplorchis taichui populations using the coi genetic marker
Journal of Helminthology, 2013Co-Authors: Do Trung Dung, Urusa Thaenkham, Jitra WaikagulAbstract:Adults of the fish-borne intestinal trematode species Haplorchis taichui were collected from humans in three provinces of Vietnam: Ha Giang, Thanh Hoa and Quang Tri. Genetic analysis revealed three groups of the parasite from clustering dendrograms, correlating with the localities in which they were collected. Measurements of evolutionary divergence over sequence pairs were greater between the different populations than within them, which indicated that the three populations were genetically different. The significance (Fst= 0.73; P value < 0.05) of the genetic variation of the three studied populations implied that genetic separation of the populations had already occurred, which may have been caused by a low gene flow among the different H. taichui populations. Factors contributing to the low gene flow may include isolation resulting from the intermediate-host fish rarely being sold outside of the rural commune where they are raised and the enclosed aquacultural areas themselves.
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Haplorchis taichui as a possible etiologic agent of irritable bowel syndrome like symptoms
Korean Journal of Parasitology, 2010Co-Authors: Dorn Watthanakulpanich, Jitra Waikagul, Wanna Maipanich, Surapol Sanguankiat, Supaporn Nuamtanong, Somchit Pubampen, Rangson Praevanit, Srisuchat Mongkhonmu, Yukifumi NawaAbstract:The aim of this study is to clarify the clinical features of Haplorchis taichui infection in humans in Nan Province, Thailand, and to correlate the clinical features with irritable bowel syndrome (IBS)-like symptoms. In this study area, only H. taichui, but neither other minute intestinal flukes nor small liver flukes were endemic. The degree of infection was determined by fecal egg counts and also by collecting adult worms after deworming. The signs and symptoms of individual patients together with their hematological and biochemical laboratory data were gathered to evaluate the relationship between the clinical features and the severity of infection. Special emphasis was made to elucidate the possible similarities of the clinical features of H. taichui infection and IBS-like symptoms. The results showed useful clinical information and the significant (> 50%) proportion of haplorchiasis patients complained of abdominal pain, lassitude, and flatulence, which were the important diagnostic symptoms of IBS. This study has reported a possible link between H. taichui and IBS, and H. taichui might probably play a role in the etiology of these IBS-like symptoms.
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Systematics of the subfamily Haplorchiinae (Trematoda: Heterphyidae), based on nuclear ribosomal DNA genes and ITS2 region.
Parasitology International, 2010Co-Authors: Urusa Thaenkham, Paron Dekumyoy, Chalit Komalamisra, Megumi Sato, Trung Dung, Jitra WaikagulAbstract:Abstract Phylogenetic relationships of 6 species in the trematode subfamily Haplorchiinae were analyzed using small and large subunit of ribosomal DNA genes (18S rDNA and 28S rDNA) and internal transcribed spacer subunit II (ITS2) region as molecular markers. Maximum Likelihood and Bayesian inference analyses of combined rDNAs and ITS2 indicated a close relationship between the genera Haplorchis and Procerovum , while these two genera were distinct from Stellantchasmus falcatus . These phylogenetic relationships were consistent with the number of testes but not with the characters of the modification of the seminal vesicle or of the ventral sucker. Although three Haplorchis spp. were, together with Procerovum , in the same cluster, their mutual topology was incongruent between rDNA and ITS2 trees. Phylogenetic analyses using other molecular markers with more species are necessary to work out solid phylogenetic relationships among the species in this subfamily.
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egg laying capacity of Haplorchis taichui digenea heterophyidae in humans
Korean Journal of Parasitology, 2009Co-Authors: Megumi Sato, Wanna Maipanich, Surapol Sanguankiat, Somchit Pubampen, Teera Kusolsuk, Jitra WaikagulAbstract:Quantitative fecal egg counts represented as the number of eggs per gram of feces (EPG) are generally a reliable parameter to estimate the worm burden of intestinal and hepatic parasitoses. Although Haplorchis taichui (Digenea: Heterophyidae) is one of the most common minute human intestinal flukes, little is known about the relationship between EPG and the actual worm burden in patients or the severity of the disease. In the present study, fecal samples were collected from 25 villagers in northern Thailand before and after praziquantel treatment. The EPG values of each participant were determined by the modified cellophane thick smear method, and adult worms were collected from the whole stool after the treatment. Eggs per day per worm (EPDPW) of H. taichui were estimated 82 from egg counts and expelled worms. The EPG was not well correlated with the worm burden, and a reverse correlation was observed between the EPDPW and the worm burden.
Do Trung Dung - One of the best experts on this subject based on the ideXlab platform.
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genetic differences among Haplorchis taichui populations in indochina revealed by mitochondrial cox1 sequences
Journal of Helminthology, 2017Co-Authors: Urusa Thaenkham, Surapol Sanguankiat, Do Trung Dung, Paron Dekumyoy, Supaporn Nuamtanong, Orawan Phuphisut, Tippayarat Yoonuan, Y Vonghachack, V Y Belizario, Jitra WaikagulAbstract:Haplorchis taichui is an intestinal heterophyid fluke that is pathogenic to humans. It is widely distributed in Asia, with a particularly high prevalence in Indochina. Previous work revealed that the lack of gene flow between three distinct populations of Vietnamese H. taichui can be attributed to their geographic isolation with no interconnected river basins. To test the hypothesis that interconnected river basins allow gene flow between otherwise isolated populations of H. taichui, as previously demonstrated for another trematode, Opisthorchis viverrini, we compared the genetic structures of seven populations of H. taichui from various localities in the lower Mekong Basin, in Thailand and Laos, with those in Vietnam, using the mitochondrial cytochrome c oxidase subunit 1 (COX1) gene. To determine the gene flow between these H. taichui populations, we calculated their phylogenetic relationships, genetic distances and haplotype diversity. Each population showed very low nucleotide diversity at this locus. However, high levels of genetic differentiation between the populations indicated very little gene flow. A phylogenetic analysis divided the populations into four clusters that correlated with the country of origin. The negligible gene flow between the Thai and Laos populations, despite sharing the Mekong Basin, caused us to reject our hypothesis. Our data suggest that the distribution of H. taichui populations was incidentally associated with national borders.
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the ribosomal transcription units of Haplorchis pumilio and h taichui and the use of 28s rdna sequences for phylogenetic identification of common heterophyids in vietnam
Parasites & Vectors, 2017Co-Authors: Thanh Hoa Le, Khue Thi Nguyen, Nga Thi Bich Nguyen, Huong Thi Thanh Doan, Do Trung Dung, David BlairAbstract:Background Heterophyidiasis is now a major public health threat in many tropical countries. Species in the trematode family Heterophyidae infecting humans include Centrocestus formosanus, Haplorchis pumilio, H. taichui, H. yokogawai, Procerovum varium and Stellantchasmus falcatus. For molecular phylogenetic and systematic studies on trematodes, we need more prospective markers for taxonomic identification and classification. This study provides near-complete ribosomal transcription units (rTU) from Haplorchis pumilio and H. taichui and demonstrates the use of 28S rDNA sequences for identification and phylogenetic analysis.
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The ribosomal transcription units of Haplorchis pumilio and H. taichui and the use of 28S rDNA sequences for phylogenetic identification of common heterophyids in Vietnam
Parasites & Vectors, 2017Co-Authors: Thanh Hoa Le, Khue Thi Nguyen, Nga Thi Bich Nguyen, Huong Thi Thanh Doan, Do Trung Dung, David BlairAbstract:Background Heterophyidiasis is now a major public health threat in many tropical countries. Species in the trematode family Heterophyidae infecting humans include Centrocestus formosanus , Haplorchis pumilio , H. taichui , H. yokogawai , Procerovum varium and Stellantchasmus falcatus. For molecular phylogenetic and systematic studies on trematodes, we need more prospective markers for taxonomic identification and classification. This study provides near-complete ribosomal transcription units (rTU) from Haplorchis pumilio and H. taichui and demonstrates the use of 28S rDNA sequences for identification and phylogenetic analysis. Results The near-complete ribosomal transcription units (rTU), consisting of 18S, ITS1, 5.8S, ITS2 and 28S rRNA genes and spacers, from H. pumilio and H. taichui from human hosts in Vietnam, were determined and annotated. Sequence analysis revealed tandem repetitive elements in ITS1 in H. pumilio and in ITS2 in H. taichui. A phylogenetic tree inferred from 28S rDNA sequences of 40 trematode strains/species, including 14 Vietnamese heterophyid individuals, clearly confirmed the status of each of the Vietnamese species: Centrocestus formosanus, Haplorchis pumilio , H. taichui , H. yokogawai , Procerovum varium and Stellantchasmus falcatus . However, the family Heterophyidae was clearly not monophyletic, with some genera apparently allied with other families within the superfamily Opisthorchioidea (i.e. Cryptogonimidae and Opisthorchiidae). These families and their constituent genera require substantial re-evaluation using a combination of morphological and molecular data. Our new molecular data will assist in such studies. Conclusions The 28S rDNA sequences are conserved among individuals within a species but varied between genera. Based on analysis of 40 28S rDNA sequences representing 19 species in the superfamily Opisthorchioidea and an outgroup taxon ( Alaria alata , family Diplostomidae), six common human pathogenic heterophyids were identified and clearly resolved. The phylogenetic tree inferred from these sequences again confirmed anomalies in molecular placement of some members of the family Heterophyidae and demonstrates the need for reappraisal of the entire superfamily Opisthorchioidea. The new sequences provided here supplement those already available in public databases and add to the array of molecular tools that can be used for the diagnosis of heterophyid species in human and animal infections.
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genetic differences among vietnamese Haplorchis taichui populations using the coi genetic marker
Journal of Helminthology, 2013Co-Authors: Do Trung Dung, Urusa Thaenkham, Jitra WaikagulAbstract:Adults of the fish-borne intestinal trematode species Haplorchis taichui were collected from humans in three provinces of Vietnam: Ha Giang, Thanh Hoa and Quang Tri. Genetic analysis revealed three groups of the parasite from clustering dendrograms, correlating with the localities in which they were collected. Measurements of evolutionary divergence over sequence pairs were greater between the different populations than within them, which indicated that the three populations were genetically different. The significance (Fst= 0.73; P value < 0.05) of the genetic variation of the three studied populations implied that genetic separation of the populations had already occurred, which may have been caused by a low gene flow among the different H. taichui populations. Factors contributing to the low gene flow may include isolation resulting from the intermediate-host fish rarely being sold outside of the rural commune where they are raised and the enclosed aquacultural areas themselves.
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discrimination of opisthorchis viverrini from Haplorchis taichui using coi sequence marker
Acta Tropica, 2007Co-Authors: Urusa Thaenkham, Do Trung Dung, Kasidis Visetsuk, Jitra WaikagulAbstract:Abstract This study aimed to discriminate infections of two common fish-borne trematodes in Thailand, Opisthorchis viverrini from Haplorchis taichui, based on mitochondrial cytochrome c oxidase subunit I (COI) gene. Designed primers (COI-OV-Hap F&R primers) amplified partial COI fragments of O. viverrini and H. taichui with high sensitivity in different developmental stages (adult, metacercaria, and egg). Polymerase chain reaction (PCR) amplicons were generated with low genomic DNA concentration (≈10−4 ng) of O. viverrini and H. taichui at 50 and 56 °C annealing temperatures, respectively. At 50 °C, COI fragments of Clonorchis sinensis and H. taichui were also obtained, but this was less sensitive than O. viverrini. At 56 °C, only H. taichui could be amplified and discriminated from H. pumilio, H. yogokawai, O. viverrini, and C. sinensis. Between 50 and 56 °C, the PCR amplicons of H. pumilio and H. yogokawai were amplified with low specificity and low sensitivity. The genetic characters among O. viverrini, C. sinensis, and H. taichui were distinguished by PCR–RFLP method. The PCR–RFLP profiles might be useful for diagnosing mixed O. viverrini and H. taichui infections in endemic areas, and for detecting metacercariae of O. viverrini, C. sinensis and H. taichui in epidemiological surveys of infections in fish hosts.
Chalobol Wongsawad - One of the best experts on this subject based on the ideXlab platform.
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Metacercaria from freshwater fishes of Mae Sa stream, Chiang Mai, Thailand.
Southeast Asian Journal of Tropical Medicine and Public Health, 2020Co-Authors: Chalobol Wongsawad, Amnat Rojanapaibul, Mhad-arehin N, Pachanawan A, Marayong T, S. Suwattanacoupt, Rojtinnakorn J, Pheravut Wongsawad, Kanda Kumchoo, Nichapu AAbstract:Metacercaria from freshwater fishes of Mae Sa stream, Doi Suthep-Pui National Park. Chiang Mai Province were investigated between January 1997-December 1998, 3,133 fishes of 32 species were collected. The prevalence of infection of fishes was 19.087% (598/3, 133). Six genera of metacercaria were recovered as follows: Acanthostomum sp, Centrocestus caninus, Haplorchis taichui, Haplorchoides sp, Posthodiplostomum sp and Stellantchasmus falcatus. The prevalence of each species of metacercaria was observed from total number of fishes as follow: 1.149% (36/3,133), 3.753% (96/3,133), 1.883% (59/3,133), 9.735% (305/3,133), 0.096% (3/ 3,133) and 2.553% (80/3,133) respectively. The highest prevalence of parasites were observed: Acanthostomum sp was 11.111% from Trichopsis vittatus; Centrocestus caninus was 100% from Xenentodon cancila; Haplorchis taichui was 12.108% from Mystacoleucus marginatus; Haplorchoides sp was 55.532% from Mystacoleucus marginatus; Posthodiplostomum sp was 1.176% from Dermogenus pusillus; Stellanchasmus falcatus was 100% from Xenentodon cancila. Centrocestus caninus, Haplorchis taichui and Stellantchasmus falcatus have been reported from human and mammals. The rest were reported from birds, fishes and reptiles.
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Seasonal variation of metacercariae in cyprinoid fish from Kwae Noi Bamroongdan Dam, Phitsanulok Province, northern Thailand.
Southeast Asian Journal of Tropical Medicine and Public Health, 2020Co-Authors: Waraporn Noikong, Chalobol Wongsawad, Anawat PhaleeAbstract:A seasonal investigation of the occurrence of metacercariae in cyprinoid fish was conducted at the Kwae Noi Bamroongdan Dam, Phitsanulok Province, northern Thailand during December 2008 - August 2009. A total 361 cyprinoid fish from 10 species were examined by artificial digestion method evaluating for metacercariae. The metacercariae determined were Haplorchis taichui, Haplorchis pumilio, Haplorchoides sp and Centrocestus caninus. The prevalence of metacercariae ranged from 76.5% to 82.6%, with an intensity of infection of 23.4 to 36.6. H. taichui, Haplorchoides sp and C. caninus had the highest prevalence in the cool season, while H. pumilio was more prevalent during the hot season. The highest infection prevalence (100%) was seen in Rasbora metallicus, Barbodes gonionotus and R. metallicus. No significant differences in the type of metacercariae were found between seasons, indicating year-round infestation of cyprinoid fish.
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molecular identification of trematode Haplorchis taichui cercariae trematoda heterophyidae in tarebia granifera snail using its2 sequences
วารสารมหาวิทยาลัยราชภัฏยะลา, 2014Co-Authors: Suksan Chuboon, Chalobol Wongsawad, Pheravut WongsawadAbstract:Minute intestinal fluke, Haplorchis taichui , remain clinical importance, especially in the north-eastern and northern regions of Thailand. For obtaining an effective epidemiological control program, a sensitive, accurate, specific detection are required. Sequences of Internal transcribed Spacer subunit 2 were performed to identify cercarial trematodes in snail intermediate hosts.The results showed that phylogram depicting phylogenetic relationships was constructed based on combined sequence data of ITS2 and showed that, 2 distinct clusters were formed by first containing with the group of middle-larger sizes trematode while the remaining was minute size with all of them were belonged to family Heterophyidae. Within the minute size cluster, H.taichui and Pleurolophocercous cercaria were placed in the same branch which can confirm the identities of PC found in this study that they will be developed and identified to H. taichui . Additionally, results obtained in this study were effective to determine the presence of parasites in snail intermediate hosts that can be use for epidemiological monitoring, preventing management and control program. พยาธใบไมลำไสขนาดเลก Haplorchis taichui ยงคงมความสำคญทางการแพทย โดยเฉพาะในภาคตะวนออกเฉยงเหนอ และภาคเหนอของไทย การควบคมการระบาดทมประสทธภาพตองการวธการตรวจสอบทมความจำเพาะเจาะจงและแมนยำสง ลำดบนวคลโอไทดบรเวณ Internal transcribed Spacer subunit 2 (ITS2) จงถกนำมาใชในการจำแนกตวออนพยาธระยะเซอรคาเรยในหอยทเปนโฮสตกงกลาง ผลการศกษาพบวา phylogram ทแสดงความสมพนธเชงววฒนาการจากขอมลลำดบนวคลโอไทดของดเอนเอบรเวณ ITS2 สามารถแบงกลมของพยาธออกเปน 2 กลม ประกอบดวยพยาธใบไมขนาดกลางถงขนาดใหญ และพยาธใบไมขนาดเลก ซงพยาธทงหมดในกลมนจดอยในกลมพยาธใบไมลำไสขนาดเลกในวงศ Heterophyidae และภายในกลมนพบวา H. taichui และ Pleurolophocercous cercariae จดอยในแขนงเดยวกน ซงเปนการยนยนวา Pleurolophocercous cercariae จากหอย T. granifera ทพบจากการศกษาครงน จะพฒนาไปเปนพยาธ H. taichui ผลการศกษาครงนมประสทธภาพในการจำแนกตวออนพยาธใบไมระยะเซอรคาเรยในหอย ซงเปนประโยชนในการตดตามตรวจสอบและการควบคมระบาดวทยาของพยาธใบไมตอไปได
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prevalence of Haplorchis taichui in field collected snails a molecular approach
Korean Journal of Parasitology, 2010Co-Authors: Thapana Chontananarth, Chalobol WongsawadAbstract:The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda: Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988 snails were collected from Mae Taeng district, Chiang Mai province, northern Thailand, which comprised of 3 species; Melanoides tuberculata, Tarebia granifera, and Thiara scabra. The overall prevalence of pleurolophocercous cercariae was 21.7% as determined by the morphology. For genetic detection of H. taichui infection in snails, 2 primers Hapt_F (5'-GGCCAACGCAATCGTCATCC-3') and Hapt_R (5'-GCGTCGGGTTTCAGACATGG-3'), were used. The genomic DNA of H. taichui, which was used as a positive control, gave an amplification of the 256 bp fragment. The overall prevalence of H. taichui from specific PCR was 9.7%. The proportion of H. taichui among the pleurolophocercous cercariae in this study was 44.9%.
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Haplorchis taichui infection of the freshwater snails and molecular identification
2010Co-Authors: Thapana Chontananarth, Chalobol WongsawadAbstract:Freshwater snails from difference microhabitat including rice filed and stream, from Mae Taeng district, Chiang Mai province, were collected during April to June 2009. A total of 193 snail samples comprised of 5 species were found. These were 60 Tarebia granifera, 38 Melanoides tuberculata, 30 Thaira scabra, 37 Filopaludina martensi martensi, and 28 Bithynia siamensis siamensis. The cercaraie were investigated by crushing method where they were classifieds into 4 types: pleurolophocercous cercaria, distome cercaria, transversotrema cercaria and monostome cercaria. The cercarial infection rates in above five species of snail were 48.33% (28:60), 47.36% (18:38), 13.33% (4:30), 0.00% (0:30), and 21.42% (8:28), respectively. The molecular identification using the 256 bp amplicon specific-polymerase chain reactions (specific - PCR), were performed and revealed that pleurolophocercous cercariae was as an intestinal trematode, Haplochis taichui. The Hap-t_F and Hap-t_R specific primer, the 256 bp amplicon was generated, which was shown to have a positive result. The total cercarial infection rates of H. taichui were 8.30% (16:193). This study would be represented some application base on epidemiological monitoring and detections in snail intermediate hosts which usefulness for preventing, management and epidemiological control programs.
