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John Akanni O. Ojewole - One of the best experts on this subject based on the ideXlab platform.
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Properties of Harpagophytum procumbens DC (Pedaliaceae) Secondary Root Aqueous Extract
2015Co-Authors: Ismail M. Mahomed, John Akanni O. OjewoleAbstract:South Africa is blessed with a rich floral biodiversity of medicinally useful plants. One such plant is Harpagophytum procumbens DC (Family: Pedaliaceae). H. procumbens is widely used in South African traditional medicine for the treatment, management and/or control of a variety of human ailments. In the present study, the analgesic effect of H. procumbens secondary root aqueous extract was evaluated in mice, using the ‘hot-plate ’ and ‘acetic acid ’ test methods; while the antiinflammatory and antidiabetic effects of the plant’s secondary root extract were investigated in rats. Fresh egg albumin-induced pedal oedema and streptozotocin (STZ)-induced diabetes mellitus were used as experimental test models of inflammation and diabetes Diclofenac (DIC, 100 mg/kg i.p.) was used as a reference analgesic and antiinflammatory agent for comparison. Chlorpropamide (250 mg/kg p.o.) was used as a reference hypoglycaemic agent for comparison. H. procumbens root aqueous extract (HPE, 50–800 mg/kg i.p.) produced significant (p < 0.05–0.001) analgesic effects against thermally and chemically induced nociceptive pain stimuli in mice. H. procumbens root extract (HPE, 50–800 mg/kg i.p.) also produced dose-related, significant reductions (p < 0.05–0.001) of the fresh egg albumin-induced acute inflammation of the rat hind paw oedema. Furthermore, the plant extract (HPE, 50–800 mg/kg i.p.) produced dose-dependent, significant reductions (p < 0.05–0.001) in the blood glucose concentrations of both fasted normal and fasted diabetic rats. The results of this experimental animal stud
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CARDIOVASCULAR EFFECTS OF Harpagophytum procumbens DC [PEDALIACEAE] SECONDARY ROOT AQUEOUS EXTRACT IN SOME MAMMALIAN EXPERIMENTAL ANIMAL MODELS
2015Co-Authors: Ismail M. Mahomed, John Akanni O. OjewoleAbstract:In an attempt to scientifically appraise the ‘healing powers ’ and medicinal value of Harpagophytum procumbens DC root aqueous extract (HPE), and throw some light on the efficacy and safety of the medicinal plant product, the cardiovascular effects of the herb’s root aqueous extract (HPE) have been investigated in some mammalian experimental animal models. The results of this laboratory animal study indicate that relatively low to moderate doses of H. procumbens root aqueous extract (HPE, 10–400 mg/kg i. v.) produced dose-dependent hypotensive and cardio-depressant effects on systemic arterial blood pressures and heart rates of pentobarbitone-anaesthetized rats. Relatively low to high concentrations of the plant’s extract (HPE, 10–1000 Pg/ml) also produced concentration-related biphasic responses in isolated cardiac muscle strips of guinea-pigs and isolated portal veins of rats. Relatively low concentrations of the plant’s extract (HPE, 10–100 Pg/ml) always produced initial slight, transient and non-significant (P!0.05) positive chronotropic responses in isolated spontaneously-beating right atria, but significant (P0.05) positive inotropic responses in isolated electrically-driven left atria of guinea-pigs. However, moderate to high concentrations of the plant’s extrac
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OXYTOCIN-LIKE EFFECT OF Harpagophytum procumbens DC [PEDALIACEAE] SECONDARY ROOT AQUEOUS EXTRACT ON RAT ISOLATED
2015Co-Authors: Www. Africanethnomedicines. Net, Ismail M. Mahomed, John Akanni O. OjewoleAbstract:Harpagophytum procumbens DC [family: Pedaliaceae] is widely used in South African traditional medicine for the treatment, management and/or control of a variety of human ailments. Some traditional health practitioners of South Africa have claimed that H. procumbens secondary root is a useful obstetric remedy for induction or acceleration of labour, as well as for expulsion of retained placentas in pregnant women. In the present study, we have, therefore, examined the effects of Harpagophytum procumbens secondary root aqueous extract (HPE) on isolated uterine muscle strips taken from pregnant and non-pregnant, young female rats. The plant’s extract (HPE, 10–800 µg/ml) induced concentration-related, significant (P<0.05–0.001) increases in the baseline tone (basal tension), and caused powerful spontaneous, rhythmic, myogenic contractions of the oestrogen-dominated uterine muscle strips taken from stilboesterol-pretreated, non-pregnant female rats. Relatively low to high concentrations of H. procumbens secondary root aqueous extract (HPE, 10–800 µg/ml) also provoked concentration-dependent, significant (P<0.05–0.001) increases in the baseline tone (basal tension) and contracte
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PHARMACOLOGICAL EFFECTS OF Harpagophytum procumbens DC [PEDALIACEAE] SECONDARY ROOT AQUEOUS EXTRACT ON ISOLATED GASTRO-INTESTINAL TRACT MUSCLES OF THE CHICK, GUINEA-PIG AND RABBIT
2015Co-Authors: Ismail M. Mahomed, Abdon M. Nsabimana, John Akanni O. OjewoleAbstract:In an attempt to scientifically appraise the ‘healing powers ’ and medicinal value of Harpagophytum procumbens DC [family: Pedaliaceae], and throw some light on the efficacy and safety of the medicinal plant product, we have examined the pharmacological effects of the plant’s secondary root aqueous extract (HPE) on the chick, guinea-pig and rabbit isolated gastro-intestinal smooth muscle preparations. The results of this laboratory animal study indicate that relatively low to high doses of Harpagophytum procumbens secondary root aqueous extract (HPE, 10–1000 µg/ml) provoked concentration-related, significant (P<0.05–0.001), atropine-sensitive contractions of the chick isolated oesophagus and guinea-pig isolated ileum. Relatively moderate to high concentrations of the plant’s extract (HPE, 200–1000 µg/ml) usually induced concentration-dependent, biphasic responses in the rabbit isolated duodenum. Relatively low concentrations of the plant’s extract (HPE, 10–100 µg/ml) usually produced an initial slight, transient and non-significant (P>0.05) increase in the amplitude of the spontaneous, myogenic, rhythmic, pendular contractions of the rabbit duodenal muscle pr hand
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Anticonvulsant activity of Harpagophytum procumbens DC [Pedaliaceae] secondary root aqueous extract in mice.
Brain Research Bulletin, 2006Co-Authors: Ismail M. Mahomed, John Akanni O. OjewoleAbstract:Abstract Harpagophytum procumbens DC [family: Pedaliaceae] is widely used in South African traditional medicine for the treatment, management and/or control of a variety of human ailments. In the present study, we have examined the anticonvulsant activity of Harpagophytum procumbens secondary root aqueous extract (HPE, 50–800 mg/kg i.p.) against pentylenetetrazole (PTZ)-, picrotoxin (PCT)- and bicuculline (BCL)-induced seizures in mice. Phenobarbitone and diazepam were used as reference anticonvulsant drugs for comparison. Like the reference anticonvulsant agents used, H. procumbens secondary root aqueous extract (HPE, 100–800 mg/kg i.p.) significantly (P
Elisabetta Chieli - One of the best experts on this subject based on the ideXlab platform.
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effects of devil s claw Harpagophytum procumbens on the multidrug transporter abcb1 p glycoprotein
Phytomedicine, 2009Co-Authors: Nadia Romiti, Gianfranco Tramonti, Alessandro Corti, Elisabetta ChieliAbstract:Abstract Devil's Claw ( Harpagophytum procumbens ) a plant native to Southern Africa, has historically been used in traditional medicine to treat a wide range of diseases and currently is widely employed as anti-inflammatory and pain-relieving natural remedy in Europe and other parts of the world. Aim of the study Little is known about possible herb-drug interactions arising from effects of Devil's Claw on the major drug metabolizing enzymes or transporters. This study evaluated in vitro the effects of Devil's Claw on the multidrug transporter ABCB1/P-glycoprotein. Materials and methods The effects of three commercially available Devil's Claw preparations and that of pure harpagoside were studied in the human kidney (HK-2) proximal tubule cell line, constitutively expressing ABCB1/P-glycoprotein (P-gp). Pgp activity and expression were tested by the calcein-AM test and by Western blotting, respectively. Results Commercial preparations inhibited P-gp activity, even if to a different extent, while pure harpagoside was almost ineffective. In cells cultured for three days in the presence of Devil's Claw preparations or pure harpagoside, a dose-dependent P-gp upregulation was found. Conclusions Our results demonstrate for the first time that Devil's Claw may interact with the multidrug transporter ABCB1/P-gp, the effect not appearing strictly related to the harpagoside relative content. Modulation of both P-gp activity and P-gp expression by Devil's Claw raise the possibility of herb-drug interactions, to be further explored in depth.
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Effects of Devil's Claw (Harpagophytum procumbens) on the multidrug transporter ABCB1/P-glycoprotein.
Phytomedicine, 2009Co-Authors: Nadia Romiti, Gianfranco Tramonti, Alessandro Corti, Elisabetta ChieliAbstract:Abstract Devil's Claw ( Harpagophytum procumbens ) a plant native to Southern Africa, has historically been used in traditional medicine to treat a wide range of diseases and currently is widely employed as anti-inflammatory and pain-relieving natural remedy in Europe and other parts of the world. Aim of the study Little is known about possible herb-drug interactions arising from effects of Devil's Claw on the major drug metabolizing enzymes or transporters. This study evaluated in vitro the effects of Devil's Claw on the multidrug transporter ABCB1/P-glycoprotein. Materials and methods The effects of three commercially available Devil's Claw preparations and that of pure harpagoside were studied in the human kidney (HK-2) proximal tubule cell line, constitutively expressing ABCB1/P-glycoprotein (P-gp). Pgp activity and expression were tested by the calcein-AM test and by Western blotting, respectively. Results Commercial preparations inhibited P-gp activity, even if to a different extent, while pure harpagoside was almost ineffective. In cells cultured for three days in the presence of Devil's Claw preparations or pure harpagoside, a dose-dependent P-gp upregulation was found. Conclusions Our results demonstrate for the first time that Devil's Claw may interact with the multidrug transporter ABCB1/P-gp, the effect not appearing strictly related to the harpagoside relative content. Modulation of both P-gp activity and P-gp expression by Devil's Claw raise the possibility of herb-drug interactions, to be further explored in depth.
Carmela Parenti - One of the best experts on this subject based on the ideXlab platform.
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Harpagophytum procumbens extract potentiates morphine antinociception in neuropathic rats
Natural Product Research, 2016Co-Authors: Carmela Parenti, Giuseppina Aricò, Marzio Pennisi, Alessandro Venditti, Giovanna M. ScotoAbstract:The association of opioids and non-steroidal anti-inflammatory drugs, to enhance pain relief and reduce the development of side effects, has been demonstrated. Given many reports concerning the antinociceptive and anti-inflammatory effects of Harpagophytum procumbens extracts, the aim of our study was to investigate the advantage of a co-administration of a subanalgesic dose of morphine preceded by a low dose of H. procumbens to verify this therapeutically useful association in a neuropathic pain model. Time course, registered with the association of the natural extract, at a dose that does not induce an antinociceptive effect, followed by a subanalgesic dose of morphine showed a well-defined antiallodynic and antihyperalgesic effect, suggesting a synergism as a result of the two-drug association. H. procumbens cooperates synergistically with morphine in resolving hyperalgesia and allodynia, two typical symptoms of neuropathic pain. The results support the strategy of using an adjuvant drug to improve opioid analgesic efficacy.
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involvement of the heme oxygenase pathway in the antiallodynic and antihyperalgesic activity of Harpagophytum procumbens in rats
Molecules, 2015Co-Authors: Carmela Parenti, Giuseppina Aricò, Santina Chiechio, Giulia Di Benedetto, Rosalba Parenti, G M ScotoAbstract:Harpagophytum procumbens (H. procumbens), also known as Devil’s Claw, has been used to treat a wide range of pathological conditions, including pain, arthritis and inflammation. Inflammatory mediators, released at the site of injury, can sensitize nociceptive terminals and are responsible for allodynia and hyperalgesia. Carbon monoxide (CO), produced in a reaction catalyzed by the enzyme heme oxygenase (HO), may play a role in nociceptive processing and has also been recognized to act as a neurotransmitter or neuromodulator in the nervous system. This study was designed to investigate whether the HO/CO pathway is involved in the analgesic response of H. procumbens in carrageenan-induced hyperalgesia in rats. Mechanical allodynia and thermal hyperalgesia were evaluated by using von Frey filaments and the plantar test, respectively. The results of our experiments showed that pretreatment with the HO inhibitor ZnPP IX significantly decreased the antihyperalgesic effect produced by H. procumbens (800 mg/kg, i.p.) in carrageenan-injected rats. Consistently, the pretreatment with hemin, a HO-1 substrate, or CORM-3, a CO releasing molecule, before a low dose of H. procumbens (300 mg/kg, i.p.) induced a clear antiallodynic response in carrageenan injected rats. These results suggest the involvement of HO-1/CO system in the antiallodynic and antihyperalgesic effect of H. procumbens in carrageenan-induced inflammatory pain.
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Harpagophytum procumbens extract potentiates morphine antinociception in neuropathic rats
Natural Product Research, 2015Co-Authors: Carmela Parenti, Giuseppina Aricò, Marzio Pennisi, Alessandro Venditti, Giovanna M. ScotoAbstract:The association of opioids and non-steroidal anti-inflammatory drugs, to enhance pain relief and reduce the development of side effects, has been demonstrated. Given many reports concerning the antinociceptive and anti-inflammatory effects of Harpagophytum procumbens extracts, the aim of our study was to investigate the advantage of a co-administration of a subanalgesic dose of morphine preceded by a low dose of H. procumbens to verify this therapeutically useful association in a neuropathic pain model. Time course, registered with the association of the natural extract, at a dose that does not induce an antinociceptive effect, followed by a subanalgesic dose of morphine showed a well-defined antiallodynic and antihyperalgesic effect, suggesting a synergism as a result of the two-drug association. H. procumbens cooperates synergistically with morphine in resolving hyperalgesia and allodynia, two typical symptoms of neuropathic pain. The results support the strategy of using an adjuvant drug to improve opi...
Sigrun Chrubasik - One of the best experts on this subject based on the ideXlab platform.
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micro morphometrical assessment of the effect of Harpagophytum procumbens extract on articular cartilage in rabbits with experimental osteoarthritis using magnetic resonance imaging
Phytotherapy Research, 2011Co-Authors: L. Wachsmuth, E. Lindhorst, S. Wrubel, H. Hadzhiyski, Martin Hudelmaier, Felix Eckstein, Sigrun ChrubasikAbstract:In vitro effects indicate a putative beneficial effect of Harpagophytum procumbens on cartilage turnover, however, in vivo protective effects on cartilage have yet to be demonstrated. A 7.1T MRI scanner was used to derive measurements of thickness, surface area and volume of the tibial condylar cartilage and to assess their precision (in the case of volume also accuracy) against the volumes of dissected cartilage measured by water displacement. Quantitative measurements were made in 16 rabbits, 6 months after unilateral medial meniscectomy and transection of the anterior cruciate ligament, after which eight of these were given a proprietary extract of Harpagophytum procumbens (HP). A semiquantitative MRI-based grading of the tibial cartilage was also compared with a ‘macroscopic’ grading based on direct visual inspection of dissected joints. The test–retest precision for MRI-based measurement was ≤6.4%. MRI-based measurements correlated well with volumes of surgically resected cartilage (r = 0.97, pair-wise random difference 4.2%). The medial tibial cartilage thickness and volume were about 35% smaller in the operated knees than in the non-operated contralateral knees (p < 0.05). The findings suggest that MRI is a precise and accurate tool for evaluating cartilage in a rabbit model of OA. The difference between the intact and operated knee in thickness and volume of the medial tibial cartilage was slightly but not significantly smaller in the HP-treated group than in the non-treated group. Copyright © 2011 John Wiley & Sons, Ltd.
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Micro-Morphometrical Assessment of the Effect of Harpagophytum procumbens Extract on Articular Cartilage in Rabbits with Experimental Osteoarthritis using Magnetic Resonance Imaging
Phytotherapy Research, 2011Co-Authors: L. Wachsmuth, E. Lindhorst, S. Wrubel, H. Hadzhiyski, Martin Hudelmaier, Felix Eckstein, Sigrun ChrubasikAbstract:In vitro effects indicate a putative beneficial effect of Harpagophytum procumbens on cartilage turnover, however, in vivo protective effects on cartilage have yet to be demonstrated. A 7.1T MRI scanner was used to derive measurements of thickness, surface area and volume of the tibial condylar cartilage and to assess their precision (in the case of volume also accuracy) against the volumes of dissected cartilage measured by water displacement. Quantitative measurements were made in 16 rabbits, 6 months after unilateral medial meniscectomy and transection of the anterior cruciate ligament, after which eight of these were given a proprietary extract of Harpagophytum procumbens (HP). A semiquantitative MRI-based grading of the tibial cartilage was also compared with a ‘macroscopic’ grading based on direct visual inspection of dissected joints. The test–retest precision for MRI-based measurement was ≤6.4%. MRI-based measurements correlated well with volumes of surgically resected cartilage (r = 0.97, pair-wise random difference 4.2%). The medial tibial cartilage thickness and volume were about 35% smaller in the operated knees than in the non-operated contralateral knees (p
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Zum Wirkstoffgehalt in Arzneimitteln aus Harpagophytum procumbens
Complementary Medicine Research, 1996Co-Authors: Sigrun Chrubasik, F. Sporer, M. WinkAbstract:31 Arzneimittel aus der Teufelskralle (Harpagophytum procumbens) von verschiedenen Herstellern wurden mittels HPLC qualitativ und quantitativ analysiert. Danach besitzen die untersu
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Effectiveness of Harpagophytum procumbens in treatment of acute low back pain.
Phytomedicine, 1996Co-Authors: Sigrun Chrubasik, Ch. Zimpfer, U. Schütt, R. ZieglerAbstract:In a study that indicates more definitive investigation is needed, 118 patients with chronic back problems seeking treatment for acute attacks of pain were included in a 4-week randomised double-blinded study on the safety and effectiveness of an extract of Harpagophytum procumbens. Both, the treatment and the placebo were administered in the form of two tablets taken three times per day; the treatment group had a daily consumption equal to 6,000 mg of crude preparation (50 mg harpagoside, the putative active ingredient). The treatment and placebo groups were well matched in physical characteristics; in the severity, duration, nature and accompaniments of their pain; and in laboratory indices of organ system function. 109 patients completed the study. The study was originally designed to measure Harpagophytum's effectiveness by measuring the use of supplementary pain-killer Tramadol over its final 3 weeks. However, this did not differ between the Harpagophytum and placebo groups nor was the consumption closely related to the amount of pain. Further analysis, though, revealed that 9 out of 51 patients who received the extract were pain free at the end of treatment compared to only 1 out of 54 patients who received placebo. A modification of the Arhus index was used as an additional measure, covering the more global impact. The percentage change was greater in those patients who received Harpagophytum extract than in those who received placebo, but inferential testing (Mann Whitney) allowed only 94% degree of confidence that this had not arisen by chance. The Arhus index reduction was based on improvement in pain. This indication of effectiveness, and the absence of demonstrable adverse effects show that more definite clinical studies of Harpagophytum extract will be worthwhile.
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Zum Harpagosidgehalt verschiedener Trockenextraktpulver aus Harpagophytum procumbens
Complementary Medicine Research, 1996Co-Authors: Sigrun Chrubasik, F. Sporer, M. WinkAbstract:Zur Bestimmung von Harpagosid in Trockenextrakten aus Harpagophytum procumbens verschiedener Hersteller wurde ein HPLC-Verfahren mit UV-Detektion (280 nm) genutzt. Die Prazision (Variationskoeffizient) der Messungen betrug 0,02%, Wiederfindungsrate bei der Aufarbeitung 99–102%. Es zeigte sich, dass der Harpagosidgehalt in den Extrakten um mehr als 100% differiert und dass jeder Extrakt ein charakteristisches Spektrum an anderen Komponenten besitzt. Das Extraktkomponentenmuster wird durch die Verwendung von Wasser, Ethanol oder Methanol zur Extraktion nicht wesentlich beeinflusst.
Tankred Wegener - One of the best experts on this subject based on the ideXlab platform.
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Devil's Claw (Harpagophytum procumbens): an anti-inflammatory herb with therapeutic potential
Phytochemistry Reviews, 2005Co-Authors: G.p. Mcgregor, Bernd Fiebich, Andrea Wartenberg, Sarah Brien, George Lewith, Tankred WegenerAbstract:Extracts of the secondary roots of the southern African plant, Devil’s Claw (Harpagophytum procumbens) provide a herbal drug with a variety of traditional indications. One area of its use that has become very popular in recent years is in the treatment of inflammatory disorders of the musculoskeletal system and of low back pain. There have been several clinical studies recently published that generally support its use in treating osteoarthritis although more studies are required in order to establish this drug as a definite therapeutic option. Here in this review, the pharmacological properties of Devil’s Claw are reviewed in detail and the clinical evidence is briefly summarised. There is good in vitro and in vivo pharmacological evidence of the anti-inflammatory and analgesic properties of this drug, although some negative findings have also been reported. Generally, the pharmacological properties of Devil’s Claw is supportive of its therapeutic potential, but more evidence from clinically relevant models, as well as at the cellular and molecular level, should be sought. Such studies may provide evidence in support of additional indications, both traditional and novel. The clinical data on Devil’s Claw is also very promising.
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Devil’s Claw (Harpagophytum procumbens): An Anti-Inflammatory Herb with Therapeutic Potential
Phytochemistry Reviews, 2005Co-Authors: Gerard Mcgregor, Bernd Fiebich, Andrea Wartenberg, Sarah Brien, George Lewith, Tankred WegenerAbstract:Extracts of the secondary roots of the southern African plant, Devil’s Claw ( Harpagophytum procumbens ) provide a herbal drug with a variety of traditional indications. One area of its use that has become very popular in recent years is in the treatment of inflammatory disorders of the musculoskeletal system and of low back pain. There have been several clinical studies recently published that generally support its use in treating osteoarthritis although more studies are required in order to establish this drug as a definite therapeutic option. Here in this review, the pharmacological properties of Devil’s Claw are reviewed in detail and the clinical evidence is briefly summarised. There is good in vitro and in vivo pharmacological evidence of the anti-inflammatory and analgesic properties of this drug, although some negative findings have also been reported. Generally, the pharmacological properties of Devil’s Claw is supportive of its therapeutic potential, but more evidence from clinically relevant models, as well as at the cellular and molecular level, should be sought. Such studies may provide evidence in support of additional indications, both traditional and novel. The clinical data on Devil’s Claw is also very promising.
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Therapy of degenerative diseases of the musculoskeletal system with South African devil's claw (Harpagophytum procumbens DC)
Wiener medizinische Wochenschrift (1946), 1999Co-Authors: Tankred WegenerAbstract:Extracts of the secondary tubers of Devil's Claw (Harpagophytum procumbens) are recommended for the supportive treatment of degenerative painful rheumatism. There was observed an improvement of motility and a reduction of pain sensation in several clinical studies. Pharmacological experiments have shown analgesic, antiphlogistic and antiinflammatory actions. Most important constituents are iridoid glycosides, which are supposed to contribute mainly to the observed effects. However, the entire extract has to be considered as active ingredient.
