Heart Valve Repair

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Craig A. Simmons - One of the best experts on this subject based on the ideXlab platform.

  • Porcine Umbilical Cord Perivascular Cells for Preclinical Testing of Tissue-Engineered Heart Valves.
    Tissue engineering. Part C Methods, 2021
    Co-Authors: Neda Latifi, Monica Lecce, Craig A. Simmons
    Abstract:

    Many children born with congenital Heart disease need a Heart Valve Repair or replacement. Currently available Repair materials and Valve replacements are incapable of growth, Repair, and adaptatio...

  • Porcine Umbilical Cord Perivascular Cells for Preclinical Testing of Tissue-Engineered Heart Valves.
    Tissue engineering. Part C Methods, 2021
    Co-Authors: Neda Latifi, Monica Lecce, Craig A. Simmons
    Abstract:

    Many children born with congenital Heart disease need a Heart Valve Repair or replacement. Currently available Repair materials and Valve replacements are incapable of growth, Repair, and adaptation, rendering them inadequate for growing children. Heart Valve tissue engineering (HVTE) aims to develop living replacement Valves that can meet these needs. Among numerous cell sources for in vitro HVTE, umbilical cord perivascular cells (UCPVCs) are particularly attractive because they are autologous, readily available, and have excellent regenerative capacity. As an essential step toward preclinical testing of Heart Valves engineered from UCPVCs, the goal of this study was to establish methods to isolate, expand, and promote extracellular matrix (ECM) synthesis by UCPVCs from pigs (porcine umbilical cord perivascular cells [pUCPVCs]), as a relevant preclinical model. We determined that Dulbecco's modified Eagle's medium with 20% fetal bovine serum supported isolation and substantial expansion of pUCPVCs, whereas media designed for human mesenchymal stromal cell (MSC) expansion did not. We further demonstrated the capacity of pUCPVCs to synthesize the main ECM components of Heart Valves (collagen type I, elastin, and glycosaminoglycans), with maximal collagen and elastin per-cell production occurring in serum-free culture conditions using StemMACS™ MSC Expansion Media. Altogether, these results establish protocols that enable the use of pUCPVCs as a viable cell source for preclinical testing of engineered Heart Valves. Impact statement This study establishes methods to successfully isolate, expand, and promote the synthesis of the main extracellular matrix components of Heart Valves (collagen type I, elastin, and glycosaminoglycans) by porcine umbilical cord perivascular cells (pUCPVCs). These protocols enable further evaluation of pUCPVCs as an autologous, readily available, and clinically relevant cell source for preclinical testing of pediatric tissue-engineered Heart Valves.

Neda Latifi - One of the best experts on this subject based on the ideXlab platform.

  • Porcine Umbilical Cord Perivascular Cells for Preclinical Testing of Tissue-Engineered Heart Valves.
    Tissue engineering. Part C Methods, 2021
    Co-Authors: Neda Latifi, Monica Lecce, Craig A. Simmons
    Abstract:

    Many children born with congenital Heart disease need a Heart Valve Repair or replacement. Currently available Repair materials and Valve replacements are incapable of growth, Repair, and adaptatio...

  • Porcine Umbilical Cord Perivascular Cells for Preclinical Testing of Tissue-Engineered Heart Valves.
    Tissue engineering. Part C Methods, 2021
    Co-Authors: Neda Latifi, Monica Lecce, Craig A. Simmons
    Abstract:

    Many children born with congenital Heart disease need a Heart Valve Repair or replacement. Currently available Repair materials and Valve replacements are incapable of growth, Repair, and adaptation, rendering them inadequate for growing children. Heart Valve tissue engineering (HVTE) aims to develop living replacement Valves that can meet these needs. Among numerous cell sources for in vitro HVTE, umbilical cord perivascular cells (UCPVCs) are particularly attractive because they are autologous, readily available, and have excellent regenerative capacity. As an essential step toward preclinical testing of Heart Valves engineered from UCPVCs, the goal of this study was to establish methods to isolate, expand, and promote extracellular matrix (ECM) synthesis by UCPVCs from pigs (porcine umbilical cord perivascular cells [pUCPVCs]), as a relevant preclinical model. We determined that Dulbecco's modified Eagle's medium with 20% fetal bovine serum supported isolation and substantial expansion of pUCPVCs, whereas media designed for human mesenchymal stromal cell (MSC) expansion did not. We further demonstrated the capacity of pUCPVCs to synthesize the main ECM components of Heart Valves (collagen type I, elastin, and glycosaminoglycans), with maximal collagen and elastin per-cell production occurring in serum-free culture conditions using StemMACS™ MSC Expansion Media. Altogether, these results establish protocols that enable the use of pUCPVCs as a viable cell source for preclinical testing of engineered Heart Valves. Impact statement This study establishes methods to successfully isolate, expand, and promote the synthesis of the main extracellular matrix components of Heart Valves (collagen type I, elastin, and glycosaminoglycans) by porcine umbilical cord perivascular cells (pUCPVCs). These protocols enable further evaluation of pUCPVCs as an autologous, readily available, and clinically relevant cell source for preclinical testing of pediatric tissue-engineered Heart Valves.

Marja J. A. Van Luyn - One of the best experts on this subject based on the ideXlab platform.

  • Ongoing foreign body reaction to subcutaneous implanted (heparin) modified Dacron in rats
    Journal of biomedical materials research. Part A, 2004
    Co-Authors: Paul H. J. Van Bilsen, Eliane R. Popa, Linda A. Brouwer, Judith Vincent, Catherine E. Taylor, Lou F. M. H. De Leij, Marc Hendriks, Marja J. A. Van Luyn
    Abstract:

    Dacron-containing Heart Valve Repair devices trigger chronic inflammation characterized by the presence of activated macrophages, foreign body giant cells, and capsule formation. Upon blood contact, proinflammatory proteins adsorb to the material and provide a substrate for monocyte binding and differentiation. Various heparin-coated polymers have been shown to reduce adsorption of proinflammatory proteins in vitro and in vivo. In this study, the effect of knitted, heparin-coated Dacron on the foreign body reaction was tested subcutaneously in rats. We hypothesized that the anti-inflammatory effect of heparin would reduce monocyte recruitment and differentiation and therefore limit the inflammatory reaction. An ongoing foreign body reaction, characterized by the presence of foreign body giant cells and high vascularization, was observed in uncoated as well as (heparin-)coated Dacron at up to 180 days of implantation. Also, a thin capsule was formed around each material up to this time. In conclusion, although heparin coatings might have an effect on the acute inflammatory response, we were not able to show a difference between heparin-coated and uncoated Dacron after 180 days' implantation in rats. Further research needs to be conducted to assess the difference in proinflammatory protein adsorption between the tested materials and the effect this has on the long-term foreign body reaction.

  • Ongoing foreign body reaction to subcutaneous implanted (heparin) modified Dacron in rats
    Journal of Biomedical Materials Research, 2004
    Co-Authors: Paul H. J. Van Bilsen, Eliane R. Popa, Linda A. Brouwer, Judith Vincent, Catherine E. Taylor, Lou F. M. H. De Leij, Marc Hendriks, Marja J. A. Van Luyn
    Abstract:

    Dacron-containing Heart Valve Repair devices trigger chronic inflammation characterized by the presence of activated macrophages, foreign body giant cells, and capsule formation. Upon blood contact, proinflammatory proteins adsorb to the material and provide a substrate for monocyte binding and differentiation. Various heparin-coated polymers have been shown to reduce adsorption of proinflammatory proteins in vitro and in vivo. In this study, the effect of knitted, heparin-coated Dacron on the foreign body reaction was tested subcutaneously in rats. We hypothesized that the anti-inflammatory effect of heparin would reduce monocyte recruitment and differentiation and therefore limit the inflammatory reaction. An ongoing foreign body reaction, characterized by the presence of foreign body giant cells and high vascularization, was observed in uncoated as well as (heparin-)coated Dacron at up to 180 days of implantation. Also, a thin capsule was formed around each material up to this time. In conclusion, although heparin coatings might have an effect on the acute inflammatory response, we were not able to show a difference between heparin-coated and uncoated Dacron after 180 days' implantation in rats. Further research needs to be conducted to assess the difference in proinflammatory protein adsorption between the tested materials and the effect this has on the long-term foreign body reaction. (C) 2004 Wiley Periodicals, Inc

Monica Lecce - One of the best experts on this subject based on the ideXlab platform.

  • Porcine Umbilical Cord Perivascular Cells for Preclinical Testing of Tissue-Engineered Heart Valves.
    Tissue engineering. Part C Methods, 2021
    Co-Authors: Neda Latifi, Monica Lecce, Craig A. Simmons
    Abstract:

    Many children born with congenital Heart disease need a Heart Valve Repair or replacement. Currently available Repair materials and Valve replacements are incapable of growth, Repair, and adaptatio...

  • Porcine Umbilical Cord Perivascular Cells for Preclinical Testing of Tissue-Engineered Heart Valves.
    Tissue engineering. Part C Methods, 2021
    Co-Authors: Neda Latifi, Monica Lecce, Craig A. Simmons
    Abstract:

    Many children born with congenital Heart disease need a Heart Valve Repair or replacement. Currently available Repair materials and Valve replacements are incapable of growth, Repair, and adaptation, rendering them inadequate for growing children. Heart Valve tissue engineering (HVTE) aims to develop living replacement Valves that can meet these needs. Among numerous cell sources for in vitro HVTE, umbilical cord perivascular cells (UCPVCs) are particularly attractive because they are autologous, readily available, and have excellent regenerative capacity. As an essential step toward preclinical testing of Heart Valves engineered from UCPVCs, the goal of this study was to establish methods to isolate, expand, and promote extracellular matrix (ECM) synthesis by UCPVCs from pigs (porcine umbilical cord perivascular cells [pUCPVCs]), as a relevant preclinical model. We determined that Dulbecco's modified Eagle's medium with 20% fetal bovine serum supported isolation and substantial expansion of pUCPVCs, whereas media designed for human mesenchymal stromal cell (MSC) expansion did not. We further demonstrated the capacity of pUCPVCs to synthesize the main ECM components of Heart Valves (collagen type I, elastin, and glycosaminoglycans), with maximal collagen and elastin per-cell production occurring in serum-free culture conditions using StemMACS™ MSC Expansion Media. Altogether, these results establish protocols that enable the use of pUCPVCs as a viable cell source for preclinical testing of engineered Heart Valves. Impact statement This study establishes methods to successfully isolate, expand, and promote the synthesis of the main extracellular matrix components of Heart Valves (collagen type I, elastin, and glycosaminoglycans) by porcine umbilical cord perivascular cells (pUCPVCs). These protocols enable further evaluation of pUCPVCs as an autologous, readily available, and clinically relevant cell source for preclinical testing of pediatric tissue-engineered Heart Valves.

Paul H. J. Van Bilsen - One of the best experts on this subject based on the ideXlab platform.

  • Ongoing foreign body reaction to subcutaneous implanted (heparin) modified Dacron in rats
    Journal of biomedical materials research. Part A, 2004
    Co-Authors: Paul H. J. Van Bilsen, Eliane R. Popa, Linda A. Brouwer, Judith Vincent, Catherine E. Taylor, Lou F. M. H. De Leij, Marc Hendriks, Marja J. A. Van Luyn
    Abstract:

    Dacron-containing Heart Valve Repair devices trigger chronic inflammation characterized by the presence of activated macrophages, foreign body giant cells, and capsule formation. Upon blood contact, proinflammatory proteins adsorb to the material and provide a substrate for monocyte binding and differentiation. Various heparin-coated polymers have been shown to reduce adsorption of proinflammatory proteins in vitro and in vivo. In this study, the effect of knitted, heparin-coated Dacron on the foreign body reaction was tested subcutaneously in rats. We hypothesized that the anti-inflammatory effect of heparin would reduce monocyte recruitment and differentiation and therefore limit the inflammatory reaction. An ongoing foreign body reaction, characterized by the presence of foreign body giant cells and high vascularization, was observed in uncoated as well as (heparin-)coated Dacron at up to 180 days of implantation. Also, a thin capsule was formed around each material up to this time. In conclusion, although heparin coatings might have an effect on the acute inflammatory response, we were not able to show a difference between heparin-coated and uncoated Dacron after 180 days' implantation in rats. Further research needs to be conducted to assess the difference in proinflammatory protein adsorption between the tested materials and the effect this has on the long-term foreign body reaction.

  • Ongoing foreign body reaction to subcutaneous implanted (heparin) modified Dacron in rats
    Journal of Biomedical Materials Research, 2004
    Co-Authors: Paul H. J. Van Bilsen, Eliane R. Popa, Linda A. Brouwer, Judith Vincent, Catherine E. Taylor, Lou F. M. H. De Leij, Marc Hendriks, Marja J. A. Van Luyn
    Abstract:

    Dacron-containing Heart Valve Repair devices trigger chronic inflammation characterized by the presence of activated macrophages, foreign body giant cells, and capsule formation. Upon blood contact, proinflammatory proteins adsorb to the material and provide a substrate for monocyte binding and differentiation. Various heparin-coated polymers have been shown to reduce adsorption of proinflammatory proteins in vitro and in vivo. In this study, the effect of knitted, heparin-coated Dacron on the foreign body reaction was tested subcutaneously in rats. We hypothesized that the anti-inflammatory effect of heparin would reduce monocyte recruitment and differentiation and therefore limit the inflammatory reaction. An ongoing foreign body reaction, characterized by the presence of foreign body giant cells and high vascularization, was observed in uncoated as well as (heparin-)coated Dacron at up to 180 days of implantation. Also, a thin capsule was formed around each material up to this time. In conclusion, although heparin coatings might have an effect on the acute inflammatory response, we were not able to show a difference between heparin-coated and uncoated Dacron after 180 days' implantation in rats. Further research needs to be conducted to assess the difference in proinflammatory protein adsorption between the tested materials and the effect this has on the long-term foreign body reaction. (C) 2004 Wiley Periodicals, Inc

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