The Experts below are selected from a list of 18054 Experts worldwide ranked by ideXlab platform
Fabrizio Mammano - One of the best experts on this subject based on the ideXlab platform.
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cib1 and cib2 are hiv 1 Helper Factors involved in viral entry
Scientific Reports, 2016Co-Authors: Fabrizio Mammano, Ana Godinhosantos, Joao Goncalves, Allan J. HanceAbstract:HIV-1 relies on the host-cell machinery to accomplish its replication cycle, and characterization of these Helper Factors contributes to a better understanding of HIV-host interactions and can identify potential novel antiviral targets. Here we explored the contribution of CIB2, previously identified by RNAi screening as a potential Helper Factor, and its homolog, CIB1. Knockdown of either CIB1 or CIB2 strongly impaired viral replication in Jurkat cells and in primary CD4+ T-lymphocytes, identifying these proteins as non-redundant Helper Factors. Knockdown of CIB1 and CIB2 impaired envelope-mediated viral entry for both for X4- and R5-tropic HIV-1, and both cell-free and cell-associated entry pathways were affected. In contrast, the level of CIB1 and CIB2 expression did not influence cell viability, cell proliferation, receptor-independent viral binding to the cell surface, or later steps in the viral replication cycle. CIB1 and CIB2 knockdown was found to reduce the expression of surface molecules implicated in HIV-1 infection, including CXCR4, CCR5 and integrin α4β7, suggesting at least one mechanism through which these proteins promote viral infection. Thus, this study identifies CIB1 and CIB2 as host Helper Factors for HIV-1 replication that are required for optimal receptor-mediated viral entry.
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cib1 and cib2 are hiv 1 Helper Factors and their modulation influences envelope mediated viral entry
Journal of Antivirals & Antiretrovirals, 2015Co-Authors: Ana Godinhosantos Allan Hance Joo J Gonalves, Fabrizio MammanoAbstract:H Immunodeficiency Virus type 1 (HIV-1) relies on the host cell machinery to complete its life cycle. Several Helper Factors have been identified in expectation that a better understanding of host-HIV interactions would lead to novel antiviral targets. As the characterization for many of these proteins is still limited, we aimed to depict the contribution of CIB2, previously identified as Helper Factor, as well as the potential contribution of its homolog, CIB1. Knockdown of both CIB1 and CIB2 in shRNA-transduced cell populations strongly impaired viral replication in target cells, recognizing these proteins as non-redundant HIV-1 Helper Factors. Also, a single-round cycle assay demonstrated that normal levels of CIB1 and CIB2 are required for HIV-1 envelope-mediated process. In fact, both X4 and R5 viral strains seem to be dependent on CIB1 and CIB2 expression levels, since infectivity of X4and R5-enveloped particles was affected. Moreover, CIB1and CIB2-knockdown populations displayed reduced viral fusion efficiency when compared to untransduced population, indicating virus-free entry impairment. Furthermore, virus-transfer through cell-cell contacts was decreased after co-culture of infected donor cells with CIB1and CIB2-knockdown target cells. Flow cytometry showed that surface expression of some key players of both routes of viral entry was altered in CIBs-depleted populations, namely co-receptor CXCR4 and integrin α4β7. Taken together, these studies revealed for the first time that CIB1 is a HIV-1 Helper Factor along with CIB2, and suggest that both CIB1 and CIB2 facilitate HIV-1 entry in natural target cells possibly through modulation of CXCR4 and α4β7.
Ana Godinhosantos - One of the best experts on this subject based on the ideXlab platform.
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cib1 and cib2 are hiv 1 Helper Factors involved in viral entry
Scientific Reports, 2016Co-Authors: Fabrizio Mammano, Ana Godinhosantos, Joao Goncalves, Allan J. HanceAbstract:HIV-1 relies on the host-cell machinery to accomplish its replication cycle, and characterization of these Helper Factors contributes to a better understanding of HIV-host interactions and can identify potential novel antiviral targets. Here we explored the contribution of CIB2, previously identified by RNAi screening as a potential Helper Factor, and its homolog, CIB1. Knockdown of either CIB1 or CIB2 strongly impaired viral replication in Jurkat cells and in primary CD4+ T-lymphocytes, identifying these proteins as non-redundant Helper Factors. Knockdown of CIB1 and CIB2 impaired envelope-mediated viral entry for both for X4- and R5-tropic HIV-1, and both cell-free and cell-associated entry pathways were affected. In contrast, the level of CIB1 and CIB2 expression did not influence cell viability, cell proliferation, receptor-independent viral binding to the cell surface, or later steps in the viral replication cycle. CIB1 and CIB2 knockdown was found to reduce the expression of surface molecules implicated in HIV-1 infection, including CXCR4, CCR5 and integrin α4β7, suggesting at least one mechanism through which these proteins promote viral infection. Thus, this study identifies CIB1 and CIB2 as host Helper Factors for HIV-1 replication that are required for optimal receptor-mediated viral entry.
Allan J. Hance - One of the best experts on this subject based on the ideXlab platform.
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cib1 and cib2 are hiv 1 Helper Factors involved in viral entry
Scientific Reports, 2016Co-Authors: Fabrizio Mammano, Ana Godinhosantos, Joao Goncalves, Allan J. HanceAbstract:HIV-1 relies on the host-cell machinery to accomplish its replication cycle, and characterization of these Helper Factors contributes to a better understanding of HIV-host interactions and can identify potential novel antiviral targets. Here we explored the contribution of CIB2, previously identified by RNAi screening as a potential Helper Factor, and its homolog, CIB1. Knockdown of either CIB1 or CIB2 strongly impaired viral replication in Jurkat cells and in primary CD4+ T-lymphocytes, identifying these proteins as non-redundant Helper Factors. Knockdown of CIB1 and CIB2 impaired envelope-mediated viral entry for both for X4- and R5-tropic HIV-1, and both cell-free and cell-associated entry pathways were affected. In contrast, the level of CIB1 and CIB2 expression did not influence cell viability, cell proliferation, receptor-independent viral binding to the cell surface, or later steps in the viral replication cycle. CIB1 and CIB2 knockdown was found to reduce the expression of surface molecules implicated in HIV-1 infection, including CXCR4, CCR5 and integrin α4β7, suggesting at least one mechanism through which these proteins promote viral infection. Thus, this study identifies CIB1 and CIB2 as host Helper Factors for HIV-1 replication that are required for optimal receptor-mediated viral entry.
Hsin-hung Yeh - One of the best experts on this subject based on the ideXlab platform.
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ICTV Virus Taxonomy Profile: Nanoviridae.
The Journal of general virology, 2021Co-Authors: John E Thomas, Ioana Grigoras, Bruno Gronenborn, John W. Randles, Robert M Harding, Yoshitaka Sano, Tania Timchenko, Hsin-hung YehAbstract:Nanoviridae is a family of plant viruses (nanovirids) whose members have small isometric virions and multipartite, circular, single-stranded (css) DNA genomes. Each of the six (genus Babuvirus) or eight (genus Nanovirus) genomic DNAs is 0.9-1.1 kb and is separately encapsidated. Many isolates are associated with satellite-like cssDNAs (alphasatellites) of 1.0-1.1 kb. Hosts are eudicots, predominantly legumes (genus Nanovirus), and monocotyledons, predominantly in the order Zingiberales (genus Babuvirus). Nanovirids require a virus-encoded Helper Factor for transmission by aphids in a circulative, non-propagative manner. This is a summary of the ICTV Report on the family Nanoviridae, which is available at ictv.global/report/nanoviridae.
John E Thomas - One of the best experts on this subject based on the ideXlab platform.
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ICTV Virus Taxonomy Profile: Nanoviridae.
The Journal of general virology, 2021Co-Authors: John E Thomas, Ioana Grigoras, Bruno Gronenborn, John W. Randles, Robert M Harding, Yoshitaka Sano, Tania Timchenko, Hsin-hung YehAbstract:Nanoviridae is a family of plant viruses (nanovirids) whose members have small isometric virions and multipartite, circular, single-stranded (css) DNA genomes. Each of the six (genus Babuvirus) or eight (genus Nanovirus) genomic DNAs is 0.9-1.1 kb and is separately encapsidated. Many isolates are associated with satellite-like cssDNAs (alphasatellites) of 1.0-1.1 kb. Hosts are eudicots, predominantly legumes (genus Nanovirus), and monocotyledons, predominantly in the order Zingiberales (genus Babuvirus). Nanovirids require a virus-encoded Helper Factor for transmission by aphids in a circulative, non-propagative manner. This is a summary of the ICTV Report on the family Nanoviridae, which is available at ictv.global/report/nanoviridae.