The Experts below are selected from a list of 4110 Experts worldwide ranked by ideXlab platform
Changsheng Zhao - One of the best experts on this subject based on the ideXlab platform.
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grafting of zwitterion from polysulfone membrane via surface initiated atrp with enhanced antifouling property and biocompatibility
Journal of Membrane Science, 2013Co-Authors: Huijuan Li, Tao Xiang, Changsheng ZhaoAbstract:Zwitterionic polymer of poly(sulfobetaine methacrylate) (PSBMA) was grafted from polysulfone (PSf) membrane via surface-initiated atom transfer radical polymerization (SI-ATRP). The polysulfone-graft-poly(sulfobetaine methacrylate) (PSf-g-PSBMA) membrane was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), attenuated total reflectance-Fourier transform infrared spectra (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), and static water contact angle (WCA) measurement. The membrane showed excellent protein antifouling property though the water flux decreased to some content; nevertheless, the protein rejection ratio of the modified membrane was not change. The biocompatibility of the membrane was characterized by protein adsorption, Hemolysis Assay, platelet adhesion, plasma recalcification time, activated partial thromboplastin time (APTT), thrombin time (TT) and cytotoxicity experiments, and the results indicated that the modified PSf membrane had good blood compatibility and cytocompatibility.
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grafting of zwitterion from polysulfone membrane via surface initiated atrp with enhanced antifouling property and biocompatibility
Journal of Membrane Science, 2013Co-Authors: Huijuan Li, Tao Xiang, Changsheng ZhaoAbstract:Zwitterionic polymer of poly(sulfobetaine methacrylate) (PSBMA) was grafted from polysulfone (PSf) membrane via surface-initiated atom transfer radical polymerization (SI-ATRP). The polysulfone-graft-poly(sulfobetaine methacrylate) (PSf-g-PSBMA) membrane was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), attenuated total reflectance-Fourier transform infrared spectra (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), and static water contact angle (WCA) measurement. The membrane showed excellent protein antifouling property though the water flux decreased to some content; nevertheless, the protein rejection ratio of the modified membrane was not change. The biocompatibility of the membrane was characterized by protein adsorption, Hemolysis Assay, platelet adhesion, plasma recalcification time, activated partial thromboplastin time (APTT), thrombin time (TT) and cytotoxicity experiments, and the results indicated that the modified PSf membrane had good blood compatibility and cytocompatibility.
Soodabeh Davaran - One of the best experts on this subject based on the ideXlab platform.
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bovine serum albumin an efficient biomacromolecule nanocarrier for improving the therapeutic efficacy of chrysin
Journal of Molecular Liquids, 2018Co-Authors: Hamed Nosrati, Marziyeh Salehiabar, Hamidreza Kheiri Manjili, Hossein Danafar, Akram Rakhshbahar, Saeed Afroogh, Soodabeh DavaranAbstract:Abstract This study manipulated a chrysin loaded bovine serum albumin nanoparticles (BSA NPs), which could solubilize the poorly water-soluble drug and increase the therapeutic efficacy of the drug. Chrysin (5, 7-dihydroxyflavone) is a natural flavonoid which have some significant biological effects on the processes of chemical defense. Chrysin loaded bovine serum albumin nanoparticles (Chrysin-BSA NPs) were synthesized by a simple desolvation procedure. The resultant Chrysin-BSA NPs showed a spherical shape, with a diameter of 97.5 ± 5.75 nm (mean ± SD) nm and a ζ-potential of - 11 mV. The in vitro drug release study of chrysin presented a sustained and controlled release pattern. Cellular toxicity of BSA NPs was also investigated on HFF2 cell lines. Additionally, a Hemolysis test of as prepared NPs were performed. Hemolysis Assay and cytotoxicity study results on HFF-2 cell line show that as prepared BSA NPs are biocompatible. The in vitro cytotoxicity of the nanoparticles was performed by MTT Assay on MCF-7 cancer cells. These results suggest that Chrysin-BSA NPs are a new drug delivery system for cancer therapy.
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folic acid conjugated bovine serum albumin an efficient smart and tumor targeted biomacromolecule for inhibition folate receptor positive cancer cells
International Journal of Biological Macromolecules, 2018Co-Authors: Hamed Nosrati, Faezeh Aliakbarzadeh, Hossein Danafar, Akram Rakhshbahar, Reza Abbasi, Jalil Charmi, Soodabeh DavaranAbstract:Abstract This work described a folic acid conjugated delivery of chrysin-loaded bovine serum albumin nanoparticles, which could overcome the nonspecific targeting disadvantage. Chrysin (5, 7-dihydroxyflavone) is a natural flavonoid which have some significant biological effects on the processes of chemical defense. Chrysin loaded bovine serum albumin nanoparticles (Chrysin-BSA NPs) were synthesized by a simple desolvation procedure. Afterward, folic acid (FA) was conjugated to the surface of Chrysin-BSA NPs by carbodiimide chemistry (Chrysin-BSA-FA NPs). The resultant Chrysin-BSA-FA NPs showed a spherical shape, with a hydrodynamic diameter of 97.5 ± 5.8 nm (mean ± SD) nm and a ζ-potential of −11.3 mV. The in vitro drug release study of chrysin presented a sustained and controlled release pattern. Hemolysis Assay and cytotoxicity study results on HFF-2 cell line show that as prepared BSA NPs are biocompatible. Both the Chrysin-BSA NPs and Chrysin-BSA-FA NPs prompted an enhanced cancer cell cytotoxic effect in contrast to chrysin solution. These data recommended that the folate-modified chrysin -loaded vehicle, which demonstrated better biocompatibility and potential superiority, could be a suitable cancer therapy in targeting tumors in the future.
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production of biological nanoparticles from bovine serum albumin as controlled release carrier for curcumin delivery
International Journal of Biological Macromolecules, 2018Co-Authors: Marziyeh Salehiabar, Hamed Nosrati, Elham Javani, Faezeh Aliakbarzadeh, Hamidreza Kheiri Manjili, Soodabeh Davaran, Hossein DanafarAbstract:This study described a curcumin (CUR) loaded bovine serum albumin nanoparticles (BSA@CUR NPs), which could solubilize the poorly water-soluble drug and increase the therapeutic efficacy of the drug. BSA@CUR NPs were synthesized by a simple coacervation procedure. The resultant BSA@CUR NPs showed a spherical shape, with a diameter of 92.59±16.75nm (mean ± SD) nm and a ζ-potential of - 9.19mV. The in vitro drug release study of CUR showed a sustained and controlled release pattern. Cellular toxicity of BSA NPs was also investigated on HFF2 cell lines. Additionally, a Hemolysis test of as prepared NPs were performed for investigation of hemocompatibility. Hemolysis Assay and cytotoxicity study results on HFF-2 cell line show that as prepared BSA NPs are biocompatible. The in vitro anticancer activity of the BSA@CUR NPs were performed by MTT Assay on MCF-7 cancer cells. These results suggest that BSA@CUR NPs are a new drug delivery system for cancer therapy.
Mehmet Bayindir - One of the best experts on this subject based on the ideXlab platform.
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impact of mesoporous silica nanoparticle surface functionality on hemolytic activity thrombogenicity and non specific protein adsorption
Journal of Materials Chemistry B, 2013Co-Authors: Adem Yildirim, Erol Ozgur, Mehmet BayindirAbstract:Although numerous mesoporous silica nanoparticle (MSN) drug carriers and theranostic agents with various surface functionalities have been designed in the last decade, their biocompatibility remains a matter of intensive debate. Here, we systematically evaluated interactions of a series of MSNs possessing different surface functional groups (ionic, polar, neutral, and hydrophobic) with blood constituents, in terms of their hemolytic activity, thrombogenicity, and adsorption of blood proteins on their surfaces. Using a Hemolysis Assay we showed that surface functionalization can reduce or even completely prevent the hemolytic activity of bare MSNs. We investigated thrombogenicity of MSNs by measuring prothrombin time (PT) and activated partial thromboplastin time (aPTT). We observed that none of the MSNs used in this study exhibit significant thrombogenic activity. Lastly, we examined non-specific protein adsorption on MSN surfaces using human serum albumin (HSA) and gamma globulins (γGs) and found that surface functionalization with ionic groups can greatly reduce protein adsorption. Demonstration of the surface functionalization having a crucial impact on blood compatibility might serve as a guideline for further investigation related to the design of mesoporous silica systems for biomedical applications, and shed light on research towards the ultimate goal of developing smart theranostic systems.
Huijuan Li - One of the best experts on this subject based on the ideXlab platform.
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grafting of zwitterion from polysulfone membrane via surface initiated atrp with enhanced antifouling property and biocompatibility
Journal of Membrane Science, 2013Co-Authors: Huijuan Li, Tao Xiang, Changsheng ZhaoAbstract:Zwitterionic polymer of poly(sulfobetaine methacrylate) (PSBMA) was grafted from polysulfone (PSf) membrane via surface-initiated atom transfer radical polymerization (SI-ATRP). The polysulfone-graft-poly(sulfobetaine methacrylate) (PSf-g-PSBMA) membrane was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), attenuated total reflectance-Fourier transform infrared spectra (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), and static water contact angle (WCA) measurement. The membrane showed excellent protein antifouling property though the water flux decreased to some content; nevertheless, the protein rejection ratio of the modified membrane was not change. The biocompatibility of the membrane was characterized by protein adsorption, Hemolysis Assay, platelet adhesion, plasma recalcification time, activated partial thromboplastin time (APTT), thrombin time (TT) and cytotoxicity experiments, and the results indicated that the modified PSf membrane had good blood compatibility and cytocompatibility.
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grafting of zwitterion from polysulfone membrane via surface initiated atrp with enhanced antifouling property and biocompatibility
Journal of Membrane Science, 2013Co-Authors: Huijuan Li, Tao Xiang, Changsheng ZhaoAbstract:Zwitterionic polymer of poly(sulfobetaine methacrylate) (PSBMA) was grafted from polysulfone (PSf) membrane via surface-initiated atom transfer radical polymerization (SI-ATRP). The polysulfone-graft-poly(sulfobetaine methacrylate) (PSf-g-PSBMA) membrane was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), attenuated total reflectance-Fourier transform infrared spectra (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), and static water contact angle (WCA) measurement. The membrane showed excellent protein antifouling property though the water flux decreased to some content; nevertheless, the protein rejection ratio of the modified membrane was not change. The biocompatibility of the membrane was characterized by protein adsorption, Hemolysis Assay, platelet adhesion, plasma recalcification time, activated partial thromboplastin time (APTT), thrombin time (TT) and cytotoxicity experiments, and the results indicated that the modified PSf membrane had good blood compatibility and cytocompatibility.
Joyanti Chutia - One of the best experts on this subject based on the ideXlab platform.
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penicillin impregnation on oxygen plasma surface functionalized chitosan antheraea assama silk fibroin studies of antibacterial activity and antithrombogenic property
Materials Science and Engineering: C, 2016Co-Authors: A Choudhury, Dolly Gogoi, Raghuram Kandimalla, Sanjeeb Kalita, Yogesh B Chaudhari, Mojibur R Khan, Jibon Kotoky, Joyanti ChutiaAbstract:Low temperature plasma can effectively tailor the surface properties of natural polymeric biomaterials according to the need for various biomedical applications. Non-mulberry silk, Antheraea assama silk fibroin (AASF) is a natural polymer having excellent biocompatibility and mechanical strength yet unlike mulberry silk, Bombyx mori silk fibroin, has drawn less interest in biomedical research. In the quest for developing as potential biomaterial, surface functionalization of plasma induced chitosan (Cs) grafted AASF ((AASF/O2-CS)g/O2) yarn is carried out using oxygen (O2) plasma. The (AASF/O2-CS)g/O2 yarn exhibits enhanced antithrombogenic property as well as antimicrobial activity against Gram positive (Bacillus subtilis) and Gram negative (Escherichia coli) bacteria as compared to AASF yarn. Moreover, impregnation of antibiotic drug (penicillin G sodium salt, PEN) on (AASF/O2-CS)g/O2 yarn further improves the observed properties. In-vitro Hemolysis Assay reveals that O2 plasma treatment and subsequent impregnation of PEN do not affect the hemocompatibility of AASF yarn. The present research findings demonstrate that plasma induced grafting of Cs followed by penicillin impregnation could significantly improve the potential applicability of AASF in the field of surgical research.
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controlled antibiotic releasing antheraea assama silk fibroin suture for infection prevention and fast wound healing
Surgery, 2016Co-Authors: A Choudhury, Dolly Gogoi, Raghuram Kandimalla, Sanjeeb Kalita, Yogesh B Chaudhari, Mojibur R Khan, Jibon Kotoky, Joyanti Chutia, Kasturi KalitaAbstract:Background The quest for developing silk fibroin as a biomaterial for drug release systems continues to draw research interest owing to its impressive mechanical properties as well as biocompatibility and biodegradability. The aim of this study is to develop low-temperature O 2 plasma-treated muga ( Antheraea assama ) silk fibroin (AASF) yarn impregnated with amoxicillin trihydrate as controlled antibiotic-releasing suture (AASF/O 2 /AMOX) for preventing postoperative site bacterial infection and fast wound healing. Methods In this experimental study, AASF and AASF/O 2 /AMOX sutures are used to close the surgical wounds of adult male Wistar rats of 4 months old and weighing 200–230 g. Results Surface hydrophilicity induced by O 2 plasma results in an increase in drug-impregnation efficiency of AASF/O 2 yarn by 16.7%. In vitro drug release profiles show continuous and prolonged release of AMOX from AASF/O 2 /AMOX yarn up to 336 hours. In vitro Hemolysis Assay reveals that O 2 plasma treatment and subsequent impregnation of AMOX do not affect the heertetmocompatibility of AASF yarn. The AASF/O 2 /AMOX yarn proves to be effective for in vitro growth inhibition of Staphylococcus aureus and Escherichia coli , whereas AASF offers no antibacterial activity against both types of bacteria. In vivo histopathology studies and colony-forming unit count data revealed accelerated wound healing activity of AASF/O 2 /AMOX over AASF yarn through rapid synthesis and proliferation of collagen, hair follicle, and connective tissues. Conclusion Outcomes of this work clearly demonstrate the potential use of AASF/O 2 /AMOX yarn as a controlled antibiotic-releasing suture biomaterial for superficial surgical applications.