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Jean Jacques Chomel - One of the best experts on this subject based on the ideXlab platform.
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rapid diagnosis of acute Hemorrhagic Conjunctivitis due to coxsackievirus a24 variant by real time one step rt pcr
Journal of Virological Methods, 2007Co-Authors: Nicolas Lévêque, I. L. Amine, Jean Jacques Muyembe, Remy Tcheng, Delphine Falcon, Nathalie Rivat, Jean Jacques Chomel, Helene Norder, Philippe Dussart, Maxime EugeneAbstract:Coxsackievirus A24 variant is, together with enterovirus 70 and adenoviruses, the major etiological agent involved in acute Hemorrhagic Conjunctivitis outbreaks worldwide. However, the standard virus isolation method followed by serotyping or VP1 region sequencing is time-consuming. A rapid method for the detection of coxsackievirus A24 variant from conjunctival swab specimens would be useful in the context of explosive and extensive outbreaks. A one-step real-time RT-PCR assay based on TaqMan technology was thus developed and assessed on 36 conjunctival swabs from outbreaks of Conjunctivitis in Morocco in 2004 due to a coxsackievirus A24 variant and in Corsica in 2006 due to adenovirus type 3, and 83 virus strains including 41 coxsackievirus A24 variant collected in French Guiana and Guadeloupe in 2003, in the Democratic Republic of the Congo in 2003, in Morocco in 2004 and 42 other virus species genetically close or known to be responsible for Conjunctivitis. All the conjunctival swabs from coxsackievirus A24 variant related outbreak and the 41 coxsackievirus A24 variant strains were tested positive by the RT-PCR assay within 4 h. This novel single-tube real-time RT-PCR assay is sensitive and specific, and consists in a reliable and faster alternative to the viral culture for recent and future acute Hemorrhagic Conjunctivitis outbreaks caused by coxsackievirus A24 variant.
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Two outbreaks of acute Hemorrhagic Conjunctivitis in Africa due to genotype III coxsackievirus A24 variant
European Journal of Clinical Microbiology & Infectious Diseases, 2007Co-Authors: Nicolas Lévêque, I. L. Amine, Gaëlle Cartet, A. B. Hammani, Y. C. Khazraji, Jean Jacques Muyembe, Bruno Lina, Helene Norder, Jean Jacques ChomelAbstract:Reported here are two outbreaks of acute Hemorrhagic Conjunctivitis that occurred in the Democratic Republic of the Congo and in Morocco in the summers of 2003 and 2004, respectively, with a large impact on public health. Virus was isolated from the conjunctival swabs of 30 Congolese and 20 Moroccan patients. Enterovirus-specific cytopathic effect was observed in all samples. None of the strains could be typed using a conventional neutralization assay with the Melnick intersecting pools; however, by sequencing the VP1 region, the viruses could be identified as coxsackie A24 variants. Phylogenetic analysis of the 3C protease region revealed that these strains were closely related to each other as well as to genotype III isolates detected in Korea in 2002, thus proving their worldwide spread. This is the first report of an epidemic of acute Hemorrhagic Conjunctivitis due to a coxsackievirus A24 variant in Africa since 1987 and the first ever from Morocco.
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Outbreak of acute Hemorrhagic Conjunctivitis in French Guiana and West Indies caused by coxsackievirus A24 variant: phylogenetic analysis reveals Asian import.
Journal of Medical Virology, 2005Co-Authors: Philippe Dussart, Gaëlle Cartet, Pierre Huguet, Christian Hajjar, Julie Vanderkerckhove, Karine Ferret, Bruno Lina, Nicolas Lévêque, Jacques Morvan, Jean Jacques ChomelAbstract:An outbreak of acute Hemorrhagic Conjunctivitis occurred in French Guiana between April and July 2003, with approximately 6,000 cases in the two major cities Kourou and Cayenne. Since acute Hemorrhagic Conjunctivitis is not a notifiable disease in France, there was no registration of the number of cases. Therefore, these were estimated by comparing the consumption of antibiotic eye drops and ophthalmic ointments during 2002 and 2003. The outbreak rapidly spread into the Caribbean Islands, causing an outbreak in Guadeloupe in October. Viral isolates from conjunctival swabs of 16 patients were confirmed to be enterovirus by PCR directed to the 5' UTR of the genome. The isolates could not be neutralized by the Melnick intersecting pools, but were shown to be CV-A24 variant by limited sequencing within the VP1 and 3C regions of 12 strains. Phylogenetic analysis revealed that they were similar to the genotype III strains causing outbreaks in Korea 2002 and Malaysia 2003. The previous outbreak of Conjunctivitis caused by CV-A24 in the Caribbean in the 1980s was also introduced from Asia, and disappeared after 3 years. This new introduction from Asia and its rapid spread into the Caribbean, where the infection disappeared after a few months, indicates that the CV-A24 variant has a different epidemiological pattern in this region compared to South East Asia, since it has not established an endemic infection. It had to be reintroduced from Asia, where it has been circulating since the 1970s.
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Outbreak of acute Hemorrhagic Conjunctivitis in French Guiana and West Indies caused by coxsackievirus A24 variant: phylogenetic analysis reveals Asian import.
Journal of Medical Virology, 2005Co-Authors: Philippe Dussart, Gaëlle Cartet, Pierre Huguet, Christian Hajjar, Julie Vanderkerckhove, Karine Ferret, Bruno Lina, Nicolas Lévêque, Jacques Morvan, Jean Jacques ChomelAbstract:An outbreak of acute Hemorrhagic Conjunctivitis occurred in French Guiana between April and July 2003, with approximately 6,000 cases in the two major cities Kourou and Cayenne. Since acute Hemorrhagic Conjunctivitis is not a notifiable disease in France, there was no registration of the number of cases. Therefore, these were estimated by comparing the consumption of antibiotic eye drops and ophthalmic ointments during 2002 and 2003. The outbreak rapidly spread into the Caribbean Islands, causing an outbreak in Guadeloupe in October. Viral isolates from conjunctival swabs of 16 patients were confirmed to be enterovirus by PCR directed to the 5' UTR of the genome. The isolates could not be neutralized by the Melnick intersecting pools, but were shown to be CV-A24 variant by limited sequencing within the VP1 and 3C regions of 12 strains. Phylogenetic analysis revealed that they were similar to the genotype III strains causing outbreaks in Korea 2002 and Malaysia 2003. The previous outbreak of Conjunctivitis caused by CV-A24 in the Caribbean in the 1980s was also introduced from Asia, and disappeared after 3 years. This new introduction from Asia and its rapid spread into the Caribbean, where the infection disappeared after a few months, indicates that the CV-A24 variant has a different epidemiological pattern in this region compared to South East Asia, since it has not established an endemic infection. It had to be reintroduced from Asia, where it has been circulating since the 1970s.
Lin Yi - One of the best experts on this subject based on the ideXlab platform.
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epidemiologic characteristics of acute Hemorrhagic Conjunctivitis in shandong province 2005 2013
Chinese Journal of Public Health, 2016Co-Authors: Sun Dapeng, Ding Shujun, Lin YiAbstract:Objective To examine epidemic characteristics and influencing factors of acute Hemorrhagic Conjunctivitis(AHC) and to provide a basis for the development of early prevention and health promotion strategies.Methods Data on AHC cases reported in Shandong province from 2005 through 2013 were collected and analyzed.Results A total of 4 130 AHC cases were reported in Shandong province during the period,with two outbreaks occurred in 2007 and2010.The epidemic peak occurred in every three years.Most of the cases were reported in Weihai,Qingdao,Liaocheng,and Dezhou municipality.The male to female ratio of the cases was 1.66:1.Most of cases were workers,peasants and students,and the constituent ratio of peasant cases increased yearly(χ~2=291.75,P <0.001).Of the cases,81.11%were diagnosed within three days after the onset of the disease.Conclusion The epidemic of AHC in Shandong province showed a slow upward trend and the epidemic peak occurred in every three years.Most of the AHC cases were workers,peasants and students and more AHC outbreaks took place in schools and factories with a dense population.
Ding Shu-jun - One of the best experts on this subject based on the ideXlab platform.
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Epidemiologic characteristics of acute Hemorrhagic Conjunctivitis in Shandong province,2005-2013
Chinese Journal of Public Health, 2016Co-Authors: Sun Da-peng, Ding Shu-junAbstract:Objective To examine epidemic characteristics and influencing factors of acute Hemorrhagic Conjunctivitis(AHC) and to provide a basis for the development of early prevention and health promotion strategies.Methods Data on AHC cases reported in Shandong province from 2005 through 2013 were collected and analyzed.Results A total of 4 130 AHC cases were reported in Shandong province during the period,with two outbreaks occurred in 2007 and2010.The epidemic peak occurred in every three years.Most of the cases were reported in Weihai,Qingdao,Liaocheng,and Dezhou municipality.The male to female ratio of the cases was 1.66:1.Most of cases were workers,peasants and students,and the constituent ratio of peasant cases increased yearly(χ~2=291.75,P
R.p. Deolankar - One of the best experts on this subject based on the ideXlab platform.
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outbreak of acute Hemorrhagic Conjunctivitis in maharashtra and gujarat states of india caused by coxsackie virus a 24 variant
Journal of Medical Virology, 2007Co-Authors: V Gopalkrishna, Pooja R. Patil, R.m. Kolhapure, H. Bilaiya, Pradip V. Fulmali, R.p. DeolankarAbstract:Acute Hemorrhagic Conjunctivitis is associated with enteroviruses. Among these, Coxsackie A-24 variant (CA-24) and Enterovirus-70 (EV-70) are known to cause epidemics and pandemics. An outbreak of acute Hemorrhagic Conjunctivitis occurred in August–September 2003 in Maharashtra and Gujarat states of India. The present investigation was carried out to determine the viral etiological agent associated with the epidemic. Virus isolates were obtained from 11 eye swabs of Conjunctivitis patients using HeLa/ Hep-2 cell lines. The isolates were characterized by serological and mouse pathogenecity tests, RT-PCR using enterovirus common primers (VP4-VP2), CA-24 specific primers (3C-proteinase region), EV-70 primers (VP-3) followed by sequencing, and phylogenetic analysis. The virus was characterized as a Coxsackie A-24 variant (CA-24v) and none of the isolates were found to be positive for EV-70. Sequencing of the PCR products derived from all the 11 isolates revealed 98.4% (SE 0.20) nucleotide identity within the Indian strains and 98.6% (0.50) and 94.4% (0.30) nucleotide identity respectively with the West Indies and Asian strains reported worldwide. The findings suggest that the outbreak of acute Hemorrhagic Conjunctivitis that occurred in Maharashtra and Gujarat states of India during August–September 2003 was caused by the Coxsackie A-24 variant (CA-24v). J. Med. Virol. 79: 748–753, 2007. © 2007 Wiley-Liss, Inc.
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outbreak of acute Hemorrhagic Conjunctivitis in maharashtra and gujarat states of india caused by coxsackie virus a 24 variant
Journal of Medical Virology, 2007Co-Authors: V Gopalkrishna, Pooja R. Patil, R.m. Kolhapure, H. Bilaiya, Pradip V. Fulmali, R.p. DeolankarAbstract:Acute Hemorrhagic Conjunctivitis is associated with enteroviruses. Among these, Coxsackie A-24 variant (CA-24) and Enterovirus-70 (EV-70) are known to cause epidemics and pandemics. An outbreak of acute Hemorrhagic Conjunctivitis occurred in August-September 2003 in Maharashtra and Gujarat states of India. The present investigation was carried out to determine the viral etiological agent associated with the epidemic. Virus isolates were obtained from 11 eye swabs of Conjunctivitis patients using HeLa/ Hep-2 cell lines. The isolates were characterized by serological and mouse pathogenecity tests, RT-PCR using enterovirus common primers (VP4-VP2), CA-24 specific primers (3C-proteinase region), EV-70 primers (VP-3) followed by sequencing, and phylogenetic analysis. The virus was characterized as a Coxsackie A-24 variant (CA-24v) and none of the isolates were found to be positive for EV-70. Sequencing of the PCR products derived from all the 11 isolates revealed 98.4% (SE 0.20) nucleotide identity within the Indian strains and 98.6% (0.50) and 94.4% (0.30) nucleotide identity respectively with the West Indies and Asian strains reported worldwide. The findings suggest that the outbreak of acute Hemorrhagic Conjunctivitis that occurred in Maharashtra and Gujarat states of India during August-September 2003 was caused by the Coxsackie A-24 variant (CA-24v).
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Outbreak of acute Hemorrhagic Conjunctivitis in Maharashtra and Gujarat states of India, caused by coxsackie virus A‐24 variant
Journal of Medical Virology, 2007Co-Authors: V Gopalkrishna, Pooja R. Patil, R.m. Kolhapure, H. Bilaiya, Pradip V. Fulmali, R.p. DeolankarAbstract:Acute Hemorrhagic Conjunctivitis is associated with enteroviruses. Among these, Coxsackie A-24 variant (CA-24) and Enterovirus-70 (EV-70) are known to cause epidemics and pandemics. An outbreak of acute Hemorrhagic Conjunctivitis occurred in August–September 2003 in Maharashtra and Gujarat states of India. The present investigation was carried out to determine the viral etiological agent associated with the epidemic. Virus isolates were obtained from 11 eye swabs of Conjunctivitis patients using HeLa/ Hep-2 cell lines. The isolates were characterized by serological and mouse pathogenecity tests, RT-PCR using enterovirus common primers (VP4-VP2), CA-24 specific primers (3C-proteinase region), EV-70 primers (VP-3) followed by sequencing, and phylogenetic analysis. The virus was characterized as a Coxsackie A-24 variant (CA-24v) and none of the isolates were found to be positive for EV-70. Sequencing of the PCR products derived from all the 11 isolates revealed 98.4% (SE 0.20) nucleotide identity within the Indian strains and 98.6% (0.50) and 94.4% (0.30) nucleotide identity respectively with the West Indies and Asian strains reported worldwide. The findings suggest that the outbreak of acute Hemorrhagic Conjunctivitis that occurred in Maharashtra and Gujarat states of India during August–September 2003 was caused by the Coxsackie A-24 variant (CA-24v). J. Med. Virol. 79: 748–753, 2007. © 2007 Wiley-Liss, Inc.
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Outbreak of acute Hemorrhagic Conjunctivitis in Maharashtra and Gujarat states of India, caused by coxsackie virus A‐24 variant
Journal of medical virology, 2007Co-Authors: V Gopalkrishna, Pooja R. Patil, R.m. Kolhapure, H. Bilaiya, Pradip V. Fulmali, R.p. DeolankarAbstract:Acute Hemorrhagic Conjunctivitis is associated with enteroviruses. Among these, Coxsackie A-24 variant (CA-24) and Enterovirus-70 (EV-70) are known to cause epidemics and pandemics. An outbreak of acute Hemorrhagic Conjunctivitis occurred in August-September 2003 in Maharashtra and Gujarat states of India. The present investigation was carried out to determine the viral etiological agent associated with the epidemic. Virus isolates were obtained from 11 eye swabs of Conjunctivitis patients using HeLa/ Hep-2 cell lines. The isolates were characterized by serological and mouse pathogenecity tests, RT-PCR using enterovirus common primers (VP4-VP2), CA-24 specific primers (3C-proteinase region), EV-70 primers (VP-3) followed by sequencing, and phylogenetic analysis. The virus was characterized as a Coxsackie A-24 variant (CA-24v) and none of the isolates were found to be positive for EV-70. Sequencing of the PCR products derived from all the 11 isolates revealed 98.4% (SE 0.20) nucleotide identity within the Indian strains and 98.6% (0.50) and 94.4% (0.30) nucleotide identity respectively with the West Indies and Asian strains reported worldwide. The findings suggest that the outbreak of acute Hemorrhagic Conjunctivitis that occurred in Maharashtra and Gujarat states of India during August-September 2003 was caused by the Coxsackie A-24 variant (CA-24v).
Guoqing Hu - One of the best experts on this subject based on the ideXlab platform.
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application of susceptible infected recovered model in dealing with an outbreak of acute Hemorrhagic Conjunctivitis on one school campus
Chinese journal of epidemiology, 2011Co-Authors: Tianmu Chen, Qiong He, Songlin Hu, Qiqi Wang, Guoqing HuAbstract:To simulate intervention measures in controlling an outbreak of acute Hemorrhagic Conjunctivitis on one school campus by using the Susceptible-Infected-Recovered (SIR) model, to provide evidence for preparedness and response to the epidemic. Classical SIR model was used to model the epidemic. Malthusian exponential decline method was employed to estimate the infective coefficient β for interventions. The initial value of parameters was determined based on empirical data. The modeling was implemented using Matlab 7.1 software. Without interventions, the outbreak was expected to experience three phrases: (1)early stage (the first 5 days) in which the epidemic developed slowly and could be intervened easily; (2) rapid growing stage (6-15 days) in which the number of infected cases increased quickly and the epidemic could not be well controlled;and (3) medium and late stage (16 days and later) in which more than 90% of the susceptible persons were infected but the intervention measures failed to prevent the epidemic. With the implementation of interventions, the epidemic was predicted to be controlled in the early stage, under the SIR model. The simulation based on the SIR model kept an acceptable consistency with the actual development of epidemic after the implementation of intervention measures. The SIR model seemed effective in modeling interventions to the epidemic of acute Hemorrhagic Conjunctivitis in the schools. Key words: Susceptible-Infected-Recovered, model; Acute Hemorrhagic Conjunctivitis; Simulation; Intervention
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Application of Susceptible-Infected-Recovered model in dealing with an outbreak of acute Hemorrhagic Conjunctivitis on one school campus
Chinese journal of epidemiology, 2011Co-Authors: Tianmu Chen, Qiong He, Songlin Hu, Qiqi Wang, Guoqing HuAbstract:To simulate intervention measures in controlling an outbreak of acute Hemorrhagic Conjunctivitis on one school campus by using the Susceptible-Infected-Recovered (SIR) model, to provide evidence for preparedness and response to the epidemic. Classical SIR model was used to model the epidemic. Malthusian exponential decline method was employed to estimate the infective coefficient β for interventions. The initial value of parameters was determined based on empirical data. The modeling was implemented using Matlab 7.1 software. Without interventions, the outbreak was expected to experience three phrases: (1) early stage (the first 5 days) in which the epidemic developed slowly and could be intervened easily; (2) rapid growing stage (6 - 15 days) in which the number of infected cases increased quickly and the epidemic could not be well controlled; and (3) medium and late stage (16 days and later) in which more than 90% of the susceptible persons were infected but the intervention measures failed to prevent the epidemic. With the implementation of interventions, the epidemic was predicted to be controlled in the early stage, under the SIR model. The simulation based on the SIR model kept an acceptable consistency with the actual development of epidemic after the implementation of intervention measures. The SIR model seemed effective in modeling interventions to the epidemic of acute Hemorrhagic Conjunctivitis in the schools.