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Thangavel Balasubramanian - One of the best experts on this subject based on the ideXlab platform.

  • Toxicity Study of Silver Nanoparticles Synthesized from Suaeda monoica on HEp-2 Cell Line
    Avicenna journal of medical biotechnology, 2012
    Co-Authors: K. Satyavani, S. Gurudeeban, Thiruganasambandam Ramanathan, Thangavel Balasubramanian
    Abstract:

    Recently there has been fabulous excitement in the nano-biotechnological area for the study of nanoparticles synthesis using some natural biological system, which has led the growth advanced nanomaterials. This intention made us to assess the biologically synthesized silver nanoparticles from the leaf of Suaeda monoica (S.monoica) using 1 mM silver nitrate. The leaf extract of S.monoica incubated with 1 mM silver nitrate solution and characterized by UV- spectrometer and AFM. The effect of synthesized silver nanoparticles on Human Epidermoid Larynx Carcinoma Cell Line was evaluated by the MTT colorimetric technique. As a result we observed gradual change in the colour of extract from greenish to brown. The synthesized silver nanoparticles confirmed by UV at 430 nm and spherical shape identified in the range of 31 nm under AFM. The effect of silver nanoparticles on Human Epidermoid Larynx Carcinoma Cell Line exhibits a dose-dependent toxicity for the Cell tested and the viability of HEp-2 Cells decreased to 50% (IC50) at the concentration of 500 nM. Further findings will be determined the exact mechanisms of this cost effective Nano-treatments.

  • Biomedical potential of silver nanoparticles synthesized from calli Cells of Citrullus colocynthis (L.) Schrad
    Journal of nanobiotechnology, 2011
    Co-Authors: K. Satyavani, S. Gurudeeban, T. Ramanathan, Thangavel Balasubramanian
    Abstract:

    Background An increasingly common application is the use of silver nanoparticles for antimicrobial coatings, wound dressings, and biomedical devices. In this present investigation, we report, biomedical potential of silver nanopaticles synthesized from calli extract of Citrullus colocynthis on Human epidermoid larynx carcinoma (HEp -2) Cell Line.

K. Satyavani - One of the best experts on this subject based on the ideXlab platform.

  • Toxicity Study of Silver Nanoparticles Synthesized from Suaeda monoica on HEp-2 Cell Line
    Avicenna journal of medical biotechnology, 2012
    Co-Authors: K. Satyavani, S. Gurudeeban, Thiruganasambandam Ramanathan, Thangavel Balasubramanian
    Abstract:

    Recently there has been fabulous excitement in the nano-biotechnological area for the study of nanoparticles synthesis using some natural biological system, which has led the growth advanced nanomaterials. This intention made us to assess the biologically synthesized silver nanoparticles from the leaf of Suaeda monoica (S.monoica) using 1 mM silver nitrate. The leaf extract of S.monoica incubated with 1 mM silver nitrate solution and characterized by UV- spectrometer and AFM. The effect of synthesized silver nanoparticles on Human Epidermoid Larynx Carcinoma Cell Line was evaluated by the MTT colorimetric technique. As a result we observed gradual change in the colour of extract from greenish to brown. The synthesized silver nanoparticles confirmed by UV at 430 nm and spherical shape identified in the range of 31 nm under AFM. The effect of silver nanoparticles on Human Epidermoid Larynx Carcinoma Cell Line exhibits a dose-dependent toxicity for the Cell tested and the viability of HEp-2 Cells decreased to 50% (IC50) at the concentration of 500 nM. Further findings will be determined the exact mechanisms of this cost effective Nano-treatments.

  • Biomedical potential of silver nanoparticles synthesized from calli Cells of Citrullus colocynthis (L.) Schrad
    Journal of nanobiotechnology, 2011
    Co-Authors: K. Satyavani, S. Gurudeeban, T. Ramanathan, Thangavel Balasubramanian
    Abstract:

    Background An increasingly common application is the use of silver nanoparticles for antimicrobial coatings, wound dressings, and biomedical devices. In this present investigation, we report, biomedical potential of silver nanopaticles synthesized from calli extract of Citrullus colocynthis on Human epidermoid larynx carcinoma (HEp -2) Cell Line.

Kai-lai Sun - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of chromosome aberrations in the Cell derived from primary Cell culture of laryngeal carcinoma and the HEp-2 Cell Line
    Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics, 2007
    Co-Authors: Ning Kang, Jing-hai Zhang, Kai-lai Sun
    Abstract:

    OBJECTIVE To search for characteristic chromosome changes in primary laryngeal squamous Cell carcinoma (LSCC) and HEp-2 Cell Line and to realize the relationship between the cytogenetic abnormality and the pathogenetic mechanism in LSCC. METHODS The fresh resulted samples of LSCC were analyzed with an improved primary Cell culture for chromosome preparation and G-banding technique. HEp-2 Cell Line was analyzed by high resolution banding technique. Molecular cytogenetics analysis was made by chromosome 6 painting probe. RESULTS Four primary LSCC succeeded in primary Cell culture and obtained metaphases, one was tetraploid, the other three were triploid. The chromosome mode of HEp-2 Cell Line was from 68 to 75 and fifteen marker chromosomes were found. The most structural abnormalities of chromosome in primary LSCC and HEp-2 Cell Line were unbalance translocation, terminal deletion and isochromosome. The complicate aberration in chromosome 6 was common in LSCC and HEp-2. CONCLUSION 6q-, I(5p), 17p-, 5q- are considered as characteristic chomosome changs in LSCC. Fluorescence in situ hybridization (FISH) may enhance the ability of detecting complicated chromosome rearrangements and marker chromosomes, which could provide more value data to verify the chromosome characteristic aberration in LSCC.

  • STK15 Gene Overexpression, Centrosomal Amplification, and Chromosomal Instability in the Absence of STK15 Mutations in Laryngeal Carcinoma
    Cancer investigation, 2005
    Co-Authors: Jesse Li-ling, Xi Wang, Kai-lai Sun
    Abstract:

    Centrosomes regulate Cell division by forming bipolar mitotic spindles and, thus, play an essential role in the maintenance of chromosomal stability. Centrosomal amplification has been found commonly among tumor Cells. Previous studies have suggested that a STK15 (serine/threonine kinase 15) gene can induce centrosomal amplification, chromosomal instability, and Cell transformation. To investigate the role of STK15 gene abnormalities in the occurrence of centrosomal amplification and chromosomal instability, a combinatory approach has been taken to investigate the expression level and point mutations of the STK15 and centrosomal/chromosomal aberrations among 72 cases of laryngeal squamous Cell carcinoma and a representative HEp-2 Cell Line. Although no mutation was detected within its exons 6 or 7, overexpression of STK15 has been found in 47 cases (65 percent) as well as in the HEp-2 Cell Line; for the latter apparent centrosomal amplification also has been noted, with the number of centrosomes within a ...

  • Study on STK15 gene abnormality and centrosomal amplification in laryngeal carcinoma
    Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics, 2004
    Co-Authors: Xi Wang, Xu Zhao, Xing-he Sun, Kai-lai Sun
    Abstract:

    Objective To investigate STK15 gene abnormality and centrosomal amplification in laryngeal carcinoma. Methods STK15 gene mRNA expressional level was tested in 62 cases of laryngeal squamous Cell carcinoma and laryngeal squamous Cell carcinoma Cell Line HEp-2 by reverse transcription-polymerase chain reaction(RT-PCR); the mutation of STK15 gene exon 6 and exon 7 in the same tissues and Cells was detected by PCR-single strand conformation polymorphism. Immunofluorescent antibodies were used to test centrosomal amplification in HEp-2 Cell Line as an example. Results STK15 gene overexpressed in 39 cases of laryngeal carcinoma (63%) and HEp-2 Cell Line. No mutation was found in exon 6 and exon 7 of STK15 gene in the above tissues and Cells. Centrosomal amplification was apparent in HEp-2 Cell Line. The number of centrosome in a single Cell changed from 1 to 7, and HEp-2 Cells with amplified centrosomes (more than 2 in one Cell) were 11%-23%. Conclusion STK15 gene overexpression and centrosomal amplification were first found in human laryngeal squamous Cell carcinoma, which indicated that STK15 gene overexpression leading to centrosomal amplification might occur in the early stage of human laryngeal carcinogenesis and be one of the key mechanisms for the occurrence of laryngeal carcinoma.

  • Expression of STK15 gene and chromosomal instability in laryngeal carcinoma
    Yi chuan xue bao = Acta genetica Sinica, 2002
    Co-Authors: Xu Zhao, Kai-lai Sun, Zhen Zhao, Xing-he Sun
    Abstract:

    To assess the relationship between expression of STK15 gene and chromosomal instability in laryngeal squamous Cell carcinoma, RNA was extracted from 50 cases of laryngeal squamous Cell carcinoma and paired normal tissue and HEp-2 Cell Line. cDNA was synthesized through reverse transcription, which was amplified by PCR using beta-actin as contrast. The results of electrophoresis were analysed by software to examine the expression level of STK15 gene in laryngeal carcinoma; karyotype analysis of HEp-2 Cell Line as an example was performed by routine and high-resolution G-banding techniques. In the 50 cases of laryngeal carcinoma, there were 34 cases whose expression of STK15 gene in tumor was higher than paired normal tissue, occupying 68%. The difference between tumor group and contrast group was prominent by statistic analysis. The expression of STK15 gene in HEp-2 Cell Line was higher than that of beta-actin; Chromosomal instability in HEp-2 Cell Line was evident: The chromosomal number range from 43 to 84 and the chromosomal model ranged from 69 to 74. The structural abnormality was represented by 13 marker chromosomes. We discovered the overexpression of STK15 gene in laryngeal carcinoma the first time. It may caused chromosomal instability through abnormal centrosome, therefore having some effect during the occurrence and development of laryngeal carcinoma.

  • Detection of epidermal growth factor receptor gene amplification in human laryngeal carcinomas by means of fluorescence in situ hybridization
    Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics, 2000
    Co-Authors: Ning Kang, Xing-he Sun, Gao H, Kai-lai Sun
    Abstract:

    Objective To detect epidermal growth factor receptor (EGFR) gene amplification in human laryngeal carcinoma Hep 2 Cell Line and laryngeal carcinoma tissues. Methods The technique of fluorescence in situ hybridization(FISH). Results The EGFR gene amplification of a laryngeal carcinoma Hep 2 Cell Line and 5 laryngeal carcinoma tissues were detected by FISH. In the metaphase chromosome and interphase nuclei of Hep 2 Cell Line and 2 laryngeal carcinoma tissues, distinct cluster and multiple dot signals were found. In the interphase nuclei of the other 3 laryngeal carcinoma tissues, no increase in the number or extent of the hybrid signals was found. Conclusion Compared with normal diploid Cell, the EGFR gene amplification was observed at different levels ranging from 2 to 8 folds in metaphase chromosome and interphase nuclei of Hep 2 Cell Line and 2 laryngeal carcinoma tissues while no amplification was observed in the other 3 laryngeal carcinoma tissues. The results demonstrate that quantitative detection of amplified gene by FISH in the metaphase chromosome and interphase nuclei is useful for detection of laryngeal carcinoma tissues.

S. Gurudeeban - One of the best experts on this subject based on the ideXlab platform.

  • Toxicity Study of Silver Nanoparticles Synthesized from Suaeda monoica on HEp-2 Cell Line
    Avicenna journal of medical biotechnology, 2012
    Co-Authors: K. Satyavani, S. Gurudeeban, Thiruganasambandam Ramanathan, Thangavel Balasubramanian
    Abstract:

    Recently there has been fabulous excitement in the nano-biotechnological area for the study of nanoparticles synthesis using some natural biological system, which has led the growth advanced nanomaterials. This intention made us to assess the biologically synthesized silver nanoparticles from the leaf of Suaeda monoica (S.monoica) using 1 mM silver nitrate. The leaf extract of S.monoica incubated with 1 mM silver nitrate solution and characterized by UV- spectrometer and AFM. The effect of synthesized silver nanoparticles on Human Epidermoid Larynx Carcinoma Cell Line was evaluated by the MTT colorimetric technique. As a result we observed gradual change in the colour of extract from greenish to brown. The synthesized silver nanoparticles confirmed by UV at 430 nm and spherical shape identified in the range of 31 nm under AFM. The effect of silver nanoparticles on Human Epidermoid Larynx Carcinoma Cell Line exhibits a dose-dependent toxicity for the Cell tested and the viability of HEp-2 Cells decreased to 50% (IC50) at the concentration of 500 nM. Further findings will be determined the exact mechanisms of this cost effective Nano-treatments.

  • Biomedical potential of silver nanoparticles synthesized from calli Cells of Citrullus colocynthis (L.) Schrad
    Journal of nanobiotechnology, 2011
    Co-Authors: K. Satyavani, S. Gurudeeban, T. Ramanathan, Thangavel Balasubramanian
    Abstract:

    Background An increasingly common application is the use of silver nanoparticles for antimicrobial coatings, wound dressings, and biomedical devices. In this present investigation, we report, biomedical potential of silver nanopaticles synthesized from calli extract of Citrullus colocynthis on Human epidermoid larynx carcinoma (HEp -2) Cell Line.

Liang Zhou - One of the best experts on this subject based on the ideXlab platform.

  • In vivo investigation of CD133 as a putative marker of cancer stem Cells in HEp-2 Cell Line.
    Head & neck, 2009
    Co-Authors: Xu Dong Wei, Jie Tian, Lei Cheng, Liang Zhou, Jack J. Jiang, Julia K. Maccallum
    Abstract:

    Background Mounting evidence suggests that most tumors consist of a heterogeneous population of Cells with a subset population that has the exclusive tumorigenic ability. They are called cancer stem Cells (CSCs). CSCs can self-renew to generate additional CSCs and also differentiate to generate phenotypically diverse cancer Cells with limited proliferative potential. They have been identified in a variety of tumors. In this study, we identify the marker of CSCs in the established human laryngeal tumor HEp-2 Cell Line in vivo. Our in vitro experiment shown as CD133, a 5-transmembrane glycoprotein expressed in HEp-2 Cell Line. CD133 was supposed as a candidate of CSC in laryngeal carcinoma. In this study, the expression of CD133 was detected in a HEp-2 Cell Line. Applying the magnetic Cell sorting (MACS) technology, we reported the results of purifying CD133 positive Cells from a HEp-2 Cell Line. Three-type Cells' tumor-forming ability was examined in vivo to identify the marker of CSCs in HEp-2 Cell Line. Methods CD133 was selected as a putative marker of CSC in laryngeal carcinoma, HEp-2 Cell Lines. Flow cytometry was used to detect the expression of CD133 in the HEp-2 Cell Line. Immunomagnetic beads were applied to purify CD133-positive Cells. CD133(+), CD133(−) tumor Cells, and unsorted HEp-2 Cells were injected into severe combined immune deficiency (SCID) mice individually to observe tumor-forming ability. Results Only a small proportion (3.15% ± 0.83%) of Cells in the HEp-2 Cell Line express the CD133 marker. In comparison with CD133(−) tumor Cells and unsorted Cells, CD133(+) Cells possess a marked capacity for tumor formation in vivo (p

  • Investigation of CD133 as putative marker of tumor-initiating Cell in laryngeal carcinoma
    Chinese journal of otorhinolaryngology head and neck surgery, 2007
    Co-Authors: Liang Zhou, Lei Cheng, Jie Tian
    Abstract:

    Objective To detect the expression of CD133 in human established larynx tumors Cell Line,HEp-2 Cell Line and observe tumorigenicity of CD133 （ ＋ ） tumorigenic Cells, CD133 （ - ） tumorigenic Cells and unsorted HEp-2 Cells in vivo. The marker of cancer stem Cells in HEp-2 Cell Line was explored. Methods Flow cytometry was used to detect the expression of putative tumor-initiating Cell marker CD133 in HEp-2 Cell Line. The immunomagnetic beads separation was applied to purify CD133 positive Cells. CD133 （ ＋ ） tumor Cells , CD133 （ - ） tumor Cells and unsorted HEp-2 Cells were injected to severe combined immune deficiency mice individually to observe their ability of forming new tumors. To determine whether the difference in tumorigenicity in subpopulation of HEp-2 Cells was due to differences in Cell growth activity and Cell cycle. The growth of sorted Cells in vitro was observed with HE stain and analyzed Cell cycle of CD133 （ ＋ ） Cells and unsorted Cells by flow cytometry. Results Only a small proportion （3. 15 ± 0. 83 ） % of Cells in HEp-2 Cell Line express CD133. Both unsorted Cells and sorted Cells were all consistent with the character of malignant tumor. Comparison of the Cell cycle status of sorted and unsorted cancer Cells after magnetic sorting revealed that both Cells exhibited a similar Cell cycle distribution. In 20 injection sites, 16 sites contained tumor in CD133（ ＋ ） group ,whereas only 10 sites in unsorted group, 7 sites in CD133（ - ） group contained tumor. Compared with CD133 （ - ） group （ χ^2 = 8.286, P = 0. 004） and unsorted group （χ^2 = 3. 956, P = 0. 047 ）, CD133 positive Cells possessed a marked capacity for giving rise to new tumors in vivo. Conclusions CD133 is one of makers for cancer stem Cell in human larynx tumors, HEp-2 Cell Line. Identification of it provides a powerful tool to investigate the tumorigenic process in the larynx and to develoo theraoies targeted to the tumor-initiating Cell. Key words: Laryngeal neoplasms; Tumor stem Cell; Cell Line

  • CD133, one of the markers of cancer stem Cells in HEp-2 Cell Line.
    The Laryngoscope, 2007
    Co-Authors: Liang Zhou, Jie Tian, Lei Cheng, Xu Dong Wei, Jack J. Jiang
    Abstract:

    Objective: In recent years, a growing body of evidence has been reported that a tumor clone is organized as a hierarchy that originates from rare stem Cells. CD133, a Cell surface antigen, was identified as a stem Cell maker for human leukemia, brain tumors, and prostate cancer. The purpose of this study was to detect the expression of CD133, a putative marker of cancer stem Cells in the HEp-2 Cell Line, and isolate CD133 positive Cells to observe their proliferation and differentiation ability in vitro. Method: Immunocytochemical staining technology and flow cytometry were used to detect the expression of the putative stem Cell marker CD133 in a HEp-2 Cell Line. The immunomagnetic beads were applied to purify CD133 positive Cells. CD133+ tumor Cells were cultured in vitro to observe their ability to proliferate and differentiate. Results: Only a small proportion (

  • Experimental investigation of CD133 as a putative marker of tumor-initiating Cell in laryngeal carcinoma
    Chinese journal of otorhinolaryngology head and neck surgery, 2006
    Co-Authors: Liang Zhou, Lei Cheng, Jie Tian
    Abstract:

    Objective To detect the expression of CD133 in human larynx tumor Cell Line, HEp-2 Cell Line and observe proliferation and differentiation ability of CD133^＋ groups in vitro. Methods Immunocytochemical staining and flow cytometry were used to detect the expression of putative tumorinitiating Cell marker CD133 in HEp-2 Cell Line, and the selective technique of immunomagnetic beads was applied to purify CD133 positive Cells. CD133^＋ tumor Cells were cultured and their ability of proliferation and differentiation were observed in vitro. Results Only 3.22% of Cells in HEp-2 Cell Line expressed CD133. In serum-free RPMI1640, On days 3, 5 and 7, their UV absorption were 0.320,0.370 and 0.558 respectively. Compared with CD133^- Cells and control HEp-2 Cells, CD133 ^＋ Cells demonstrated increased proliferation capacity. The proportion of CD133^＋ Cells decreased in culture as days passed. In twelve days of culture, the percentage of CD133 ^＋ Cells decreased from 90.88% to 4.53 %. Conclusions CD133 was one of makers for tumor-initiating Cell of human laryngeal carcinoma, HEp-2 Cell Line. Its identification would provide a helpful tool to investigate the tumorigenic process of human laryngeal carcinoma and to develop targeted therapies. Key words: Laryngeal neoplasm; Cancer stem Cell; Tumor Cells, cultured; Antigen,CD