The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
Xiangmin Zhang - One of the best experts on this subject based on the ideXlab platform.
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gas chromatography mass spectrometric analysis of hexanal and Heptanal in human blood by headspace single drop microextraction with droplet derivatization
Analytical Biochemistry, 2005Co-Authors: Ning Li, Chunhui Deng, Xizhong Shen, Xiangmin ZhangAbstract:Abstract In this work, we developed a new approach to the analysis of the lung cancer biomarkers, hexanal and Heptanal in human blood that was based on headspace single-drop microextraction (HS-SDME) with droplet derivatization, followed by gas chromatography–mass spectrometry (GC-MS). Aldehydes in blood were headspace extracted, concentrated, and derivatized by a suspended microdrop solvent containing the derivatization agent O -(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride. The aldehyde oximes formed in the microdrop solvent were analyzed by GC-MS. The optimal HS-SDME with droplet derivatization parameters extraction solvent of decane, sample temperature of 40 °C, extraction time of 6 min, stirring rate of 1100 rpm, and solvent volume of 2.0 μL were obtained and used for analysis of hexanal and Heptanal in blood. The method reproducibility, linearity, recovery, and detection limit were studied and the obtained results demonstrated the method feasibility. Finally, the proposed method was applied to the quantification of hexanal and Heptanal in cancer blood and normal blood. Due to sample extraction, concentration, and derivatization being performed in a single step, the method provided a simple, rapid, low-cost, and efficient approach to analysis of aldehydes in blood samples.
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determination of acetone hexanal and Heptanal in blood samples by derivatization with pentafluorobenzyl hydroxylamine followed by headspace single drop microextraction and gas chromatography mass spectrometry
Analytica Chimica Acta, 2005Co-Authors: Ning Li, Chunhui Deng, Xizhong Shen, Xiangmin ZhangAbstract:Abstract In the study, we developed a simple, rapid, sensitive, and inexpensive method for determination of the disease biomarkers of acetone, hexanal and Heptanal in human blood. For the first time, derivatization of carbonyls with O -2,3,4,5,6-(pentafluorobenzyl)hydroxylamine (PFBHA) was combined with headspace single-drop microextractin (HS-SDME) and gas chromatography-mass spectrometry (GC–MS) and applied to the analysis of acetone, hexanal, and Heptanal in human blood. At first, acetone, hexanal and Heptanal in blood were derivatized with PFBHA and formed oximes in several seconds. Sequentially, the oximes were headspace extracted and concentrated by a microdrop solvent. Finally, the extracted oximes were analyzed by GC–MS. HS-SDME conditions and method validations were studied. Due to needing of only 2 μl organic solvent, short extraction time of 8 min, and simple operation, derivatization-HS-SDME was shown to be a rapid, simple, and inexpensive technique for analysis of acetone, hexanal, and Heptanal in human blood. Moreover, it had low detection limit values from 0.24 to 0.62 nM, and good reproducibility (R.S.D. less than 12%).
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development of headspace solid phase microextraction with on fiber derivatization for determination of hexanal and Heptanal in human blood
Journal of Chromatography B, 2004Co-Authors: Chunhui Deng, Ning Li, Xiangmin ZhangAbstract:Abstract Hexanal and Heptanal in human blood have been regarded as potential biomarkers of lung cancer. Owing to their high volatilities and activities, it is difficult to accurately measure the two biomarkers. In the current work, headspace solid-phase microextraction (HS-SPME) with on-fiber derivatization technique was developed for quantitative analysis of hexanal and Heptanal in human blood. In the proposed method, the two aldehydes in blood were headspace extracted by using a poly (dimethylsiloxane)/divinylbenzene (PDMS/DVB) fiber with O-2,3,4,5,6-(pentafluorobenzyl) hydroxylamine (PFBHA) at 60 °C for 8 min. The aldehyde oximes formed on the fiber were desorbed and analyzed by gas chromatography–mass spectrometry (GC–MS). The method validations including detection limit, recovery and precision were studied. It was found that the method provided low detection limits of 0.006 nM for hexanal and 0.005 nM for Heptanal, recoveries from 89% to 95% and R.S.D. values less than 8.5%. The present method was applied to quantitative analysis of hexanal and Heptanal in normal blood and lung cancer blood. Hexanal concentrations from 7.33 to 15.23 μM and Heptanal concentrations from 2.47 to 9.23 μM were found in the lung cancer blood, while both hexanal and Heptanal in the control blood were lower than 0.6 μM. This further demonstrated that hexanal and Heptanal might be the biomarkers of lung cancer. The experimental results showed that GC–MS and HS-SPME with on-fiber derivatization is a simple, rapid, sensitive and solvent-free method for determination of in hexanal and Heptanal human blood.
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Development of headspace solid-phase microextraction with on-fiber derivatization for determination of hexanal and Heptanal in human blood.
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2004Co-Authors: Chunhui Deng, Ning Li, Xiangmin ZhangAbstract:Hexanal and Heptanal in human blood have been regarded as potential biomarkers of lung cancer. Owing to their high volatilities and activities, it is difficult to accurately measure the two biomarkers. In the current work, headspace solid-phase microextraction (HS-SPME) with on-fiber derivatization technique was developed for quantitative analysis of hexanal and Heptanal in human blood. In the proposed method, the two aldehydes in blood were headspace extracted by using a poly (dimethylsiloxane)/divinylbenzene (PDMS/DVB) fiber with O-2,3,4,5,6-(pentafluorobenzyl) hydroxylamine (PFBHA) at 60 degrees C for 8 min. The aldehyde oximes formed on the fiber were desorbed and analyzed by gas chromatography-mass spectrometry (GC-MS). The method validations including detection limit, recovery and precision were studied. It was found that the method provided low detection limits of 0.006 nM for hexanal and 0.005 nM for Heptanal, recoveries from 89% to 95% and R.S.D. values less than 8.5%. The present method was applied to quantitative analysis of hexanal and Heptanal in normal blood and lung cancer blood. Hexanal concentrations from 7.33 to 15.23 microM and Heptanal concentrations from 2.47 to 9.23 microM were found in the lung cancer blood, while both hexanal and Heptanal in the control blood were lower than 0.6 microM. This further demonstrated that hexanal and Heptanal might be the biomarkers of lung cancer. The experimental results showed that GC-MS and HS-SPME with on-fiber derivatization is a simple, rapid, sensitive and solvent-free method for determination of in hexanal and Heptanal human blood.
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Investigation of volatile biomarkers in lung cancer blood using solid-phase microextraction and capillary gas chromatography-mass spectrometry.
Journal of Chromatography B, 2004Co-Authors: Chunhui Deng, Xiangmin Zhang, Ning LiAbstract:In the present work, solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) was developed for investigation of lung cancer volatile biomarkers. Headspace SPME conditions (fiber coating, extraction temperature and extraction time) and desorption conditions were optimized and applied to determination of volatiles in human blood. To find the biomarkers of lung cancer, investigation of volatile compounds in lung cancer blood and control was performed by using the present method. Concentrations of hexanal and Heptanal in lung cancer blood were found to be much higher than those in control blood. The two molecules of hexanal and Heptanal were regarded as biomarkers of lung cancer. By comparison of volatiles in breath and in blood, it is demonstrated that hexanal and Heptanal in breath were originated from blood and screening of lung cancer by breath analysis be feasible. These results show that SPME/GC-MS is a simple, rapid and sensitive method very suitable for investigation of volatile disease markers in human blood.
Eunok Choe - One of the best experts on this subject based on the ideXlab platform.
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Changes in oxidation-derived off-flavor compounds of roasted sesame oil during accelerated storage in the dark
Biocatalysis and Agricultural Biotechnology, 2012Co-Authors: Edwald Lee, Eunok ChoeAbstract:Oil oxidation and off-flavor compounds were evaluated in roasted sesame oil during accelerated storage at 70 °C in the dark for 4 weeks. Oil oxidation was monitored by measuring contents of conjugated dienoic acid (CDA) and polar compounds as well as by analyzing fatty acid composition by gas chromatography (GC). Off-flavor compounds were evaluated with the headspace gas analysis using a solid phase microextraction (SPME) and GC. The roasted sesame oil showed little change in fatty acid composition during storage, and contents of CDA and polar compounds increased slowly. Among off-flavor compounds including pentane, hexane, hexanal, Heptanal, 1-pentanol, acetic acid, and furfuryl alcohol, hexanal, Heptanal, or 1-pentanol content increased with storage time and showed a high correlation with CDA values of the oil. Acetic acid and furfuryl alcohol did not show consistent trends throughout the 4 week storage. The results confirmed high oxidative stability of roasted sesame oil and suggested use of hexanal, Heptanal, or 1-pentanol as an indicator off-flavor compound to monitor the autoxidation of roasted sesame oil. © 2011 Elsevier Ltd.
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Changes in oxidation-derived off-flavor compounds of roasted sesame oil during accelerated storage in the dark
Biocatalysis and agricultural biotechnology, 2011Co-Authors: Eunok ChoeAbstract:Abstract Oil oxidation and off-flavor compounds were evaluated in roasted sesame oil during accelerated storage at 70 °C in the dark for 4 weeks. Oil oxidation was monitored by measuring contents of conjugated dienoic acid (CDA) and polar compounds as well as by analyzing fatty acid composition by gas chromatography (GC). Off-flavor compounds were evaluated with the headspace gas analysis using a solid phase microextraction (SPME) and GC. The roasted sesame oil showed little change in fatty acid composition during storage, and contents of CDA and polar compounds increased slowly. Among off-flavor compounds including pentane, hexane, hexanal, Heptanal, 1-pentanol, acetic acid, and furfuryl alcohol, hexanal, Heptanal, or 1-pentanol content increased with storage time and showed a high correlation with CDA values of the oil. Acetic acid and furfuryl alcohol did not show consistent trends throughout the 4 week storage. The results confirmed high oxidative stability of roasted sesame oil and suggested use of hexanal, Heptanal, or 1-pentanol as an indicator off-flavor compound to monitor the autoxidation of roasted sesame oil.
Yuqi Feng - One of the best experts on this subject based on the ideXlab platform.
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determination of hexanal and Heptanal in human urine using magnetic solid phase extraction coupled with in situ derivatization by high performance liquid chromatography
Talanta, 2015Co-Authors: Bifeng Yuan, Yuqi FengAbstract:Abstract In this study, magnetic solid phase extraction coupled with in-situ derivatization (MSPE-ISD) was established for the determination of hexanal and Heptanal in human urine. 2,4-Dinitrophenylhydrazine (DNPH) was used as the derivatization reagent that was adsorbed onto the surface of magnetite/silica/poly(methacrylic acid-co-ethylene glycol dimethacrylate) (Fe3O4/SiO2/P(MAA-co-EGDMA)). And then simultaneous extraction and derivatization of the aldehydes were performed on the DNPH-adsorbed Fe3O4/SiO2/P(MAA-co-EGDMA). The simple, rapid and sensitive determination of hexanal and Heptanal can be accomplished within 9 min. Under optimized conditions, the limits of detection (LODs) were 1.7 and 2.5 nmol/L for hexanal and Heptanal, respectively. The relative recoveries ranged from 72.8% to 91.4% with the intra- and inter-day relative standard deviations (RSDs) being less than 9.6%. Furthermore, the proposed method was successfully applied to determine endogenous hexanal and Heptanal in human urine from healthy persons and lung cancer patients. The results showed the higher concentrations of hexanal and Heptanal were observed in lung cancer patients compared to healthy controls. Thus, the developed MSPE-ISD method is suitable for the determination of aldehydes in urines.
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analysis of hexanal and Heptanal in human blood by simultaneous derivatization and dispersive liquid liquid microextraction then lc apci ms ms
Chromatographia, 2009Co-Authors: Hui Xu, Dandan Song, Sheng Hu, Qiongwei Yu, Yuqi FengAbstract:A new analytical approach, simultaneous derivatization and dispersive liquid–liquid microextraction followed by liquid chromatography–atmospheric-pressure chemical ionization tandem mass spectrometry, has been developed for analysis of hexanal and Heptanal in human blood. In the derivatization and extraction procedure a solution of 2,4-dinitrophenylhydrazine (derivatization reagent) in 85 μL acetonitrile (dispersive solvent) and 50 μL tetrachloromethane (extraction solvent) was rapidly injected into the aqueous sample containing hexanal and Heptanal. Within a few seconds the aldehydes were derivatized and simultaneously extracted. After centrifugation, the hydrazones in the sediment phase were analyzed by LC–APCI–MS–MS. Derivatization and extraction conditions were investigated systematically. Under the optimum conditions enrichment factors for hexanal and Heptanal in a 1-mL sample were 63 and 73, respectively. The calibration plots were linear in the ranges 0.5–100 and 100–1,000 nmol L−1, respectively, and the respective limits of detection (LOD) were 0.17 and 0.076 nmol L−1. Reproducibility and recovery were good. The experimental results were compared with those obtained by use of solid-phase extraction and polymer monolithic microextraction. Because sample derivatization, extraction, and concentration were combined in a single step, the proposed method enabled simple, rapid, inexpensive, and efficient analysis of aldehydes in blood. The method has great potential for clinical analysis of biologically relevant aldehydes.
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polymer monolith microextraction with in situ derivatization and its application to high performance liquid chromatography determination of hexanal and Heptanal in plasma
Journal of Chromatography A, 2007Co-Authors: Huijuan Zhang, Jingfang Huang, Yuqi FengAbstract:Abstract A simple, rapid and sensitive method for the determination of hexanal and Heptanal in plasma by high-performance liquid chromatography (HPLC) has been developed, which is based on polymer monolith microextraction (PMME) with in situ derivatization. 2,4-dinitrophenylhydrazine (DNPH) as a derivatizing reagent was first adsorbed on a poly (methacrylic acid-co-ethylene glycol dimethacrylate) (MAA-EGDMA) monolith, and then microextraction was performed simultaneously with derivatization on the monolith. The several parameters affecting the in situ derivatization simultaneously with PMME were investigated, including the flow rate, pH, buffer concentration, and temperature. The whole pretreatment process can be accomplished within 8 min. The limits of detection for hexanal and Heptanal were found to be 2.4 and 3.6 nmol/L, respectively. The recoveries in plasma sample were in the range of 83–87% with the inter- and intra-day precisions less than 6.8%. This method was successfully applied to the analysis of hexanal and Heptanal in plasma samples from different cancer patients.
Ning Li - One of the best experts on this subject based on the ideXlab platform.
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gas chromatography mass spectrometric analysis of hexanal and Heptanal in human blood by headspace single drop microextraction with droplet derivatization
Analytical Biochemistry, 2005Co-Authors: Ning Li, Chunhui Deng, Xizhong Shen, Xiangmin ZhangAbstract:Abstract In this work, we developed a new approach to the analysis of the lung cancer biomarkers, hexanal and Heptanal in human blood that was based on headspace single-drop microextraction (HS-SDME) with droplet derivatization, followed by gas chromatography–mass spectrometry (GC-MS). Aldehydes in blood were headspace extracted, concentrated, and derivatized by a suspended microdrop solvent containing the derivatization agent O -(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride. The aldehyde oximes formed in the microdrop solvent were analyzed by GC-MS. The optimal HS-SDME with droplet derivatization parameters extraction solvent of decane, sample temperature of 40 °C, extraction time of 6 min, stirring rate of 1100 rpm, and solvent volume of 2.0 μL were obtained and used for analysis of hexanal and Heptanal in blood. The method reproducibility, linearity, recovery, and detection limit were studied and the obtained results demonstrated the method feasibility. Finally, the proposed method was applied to the quantification of hexanal and Heptanal in cancer blood and normal blood. Due to sample extraction, concentration, and derivatization being performed in a single step, the method provided a simple, rapid, low-cost, and efficient approach to analysis of aldehydes in blood samples.
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determination of acetone hexanal and Heptanal in blood samples by derivatization with pentafluorobenzyl hydroxylamine followed by headspace single drop microextraction and gas chromatography mass spectrometry
Analytica Chimica Acta, 2005Co-Authors: Ning Li, Chunhui Deng, Xizhong Shen, Xiangmin ZhangAbstract:Abstract In the study, we developed a simple, rapid, sensitive, and inexpensive method for determination of the disease biomarkers of acetone, hexanal and Heptanal in human blood. For the first time, derivatization of carbonyls with O -2,3,4,5,6-(pentafluorobenzyl)hydroxylamine (PFBHA) was combined with headspace single-drop microextractin (HS-SDME) and gas chromatography-mass spectrometry (GC–MS) and applied to the analysis of acetone, hexanal, and Heptanal in human blood. At first, acetone, hexanal and Heptanal in blood were derivatized with PFBHA and formed oximes in several seconds. Sequentially, the oximes were headspace extracted and concentrated by a microdrop solvent. Finally, the extracted oximes were analyzed by GC–MS. HS-SDME conditions and method validations were studied. Due to needing of only 2 μl organic solvent, short extraction time of 8 min, and simple operation, derivatization-HS-SDME was shown to be a rapid, simple, and inexpensive technique for analysis of acetone, hexanal, and Heptanal in human blood. Moreover, it had low detection limit values from 0.24 to 0.62 nM, and good reproducibility (R.S.D. less than 12%).
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development of headspace solid phase microextraction with on fiber derivatization for determination of hexanal and Heptanal in human blood
Journal of Chromatography B, 2004Co-Authors: Chunhui Deng, Ning Li, Xiangmin ZhangAbstract:Abstract Hexanal and Heptanal in human blood have been regarded as potential biomarkers of lung cancer. Owing to their high volatilities and activities, it is difficult to accurately measure the two biomarkers. In the current work, headspace solid-phase microextraction (HS-SPME) with on-fiber derivatization technique was developed for quantitative analysis of hexanal and Heptanal in human blood. In the proposed method, the two aldehydes in blood were headspace extracted by using a poly (dimethylsiloxane)/divinylbenzene (PDMS/DVB) fiber with O-2,3,4,5,6-(pentafluorobenzyl) hydroxylamine (PFBHA) at 60 °C for 8 min. The aldehyde oximes formed on the fiber were desorbed and analyzed by gas chromatography–mass spectrometry (GC–MS). The method validations including detection limit, recovery and precision were studied. It was found that the method provided low detection limits of 0.006 nM for hexanal and 0.005 nM for Heptanal, recoveries from 89% to 95% and R.S.D. values less than 8.5%. The present method was applied to quantitative analysis of hexanal and Heptanal in normal blood and lung cancer blood. Hexanal concentrations from 7.33 to 15.23 μM and Heptanal concentrations from 2.47 to 9.23 μM were found in the lung cancer blood, while both hexanal and Heptanal in the control blood were lower than 0.6 μM. This further demonstrated that hexanal and Heptanal might be the biomarkers of lung cancer. The experimental results showed that GC–MS and HS-SPME with on-fiber derivatization is a simple, rapid, sensitive and solvent-free method for determination of in hexanal and Heptanal human blood.
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Development of headspace solid-phase microextraction with on-fiber derivatization for determination of hexanal and Heptanal in human blood.
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2004Co-Authors: Chunhui Deng, Ning Li, Xiangmin ZhangAbstract:Hexanal and Heptanal in human blood have been regarded as potential biomarkers of lung cancer. Owing to their high volatilities and activities, it is difficult to accurately measure the two biomarkers. In the current work, headspace solid-phase microextraction (HS-SPME) with on-fiber derivatization technique was developed for quantitative analysis of hexanal and Heptanal in human blood. In the proposed method, the two aldehydes in blood were headspace extracted by using a poly (dimethylsiloxane)/divinylbenzene (PDMS/DVB) fiber with O-2,3,4,5,6-(pentafluorobenzyl) hydroxylamine (PFBHA) at 60 degrees C for 8 min. The aldehyde oximes formed on the fiber were desorbed and analyzed by gas chromatography-mass spectrometry (GC-MS). The method validations including detection limit, recovery and precision were studied. It was found that the method provided low detection limits of 0.006 nM for hexanal and 0.005 nM for Heptanal, recoveries from 89% to 95% and R.S.D. values less than 8.5%. The present method was applied to quantitative analysis of hexanal and Heptanal in normal blood and lung cancer blood. Hexanal concentrations from 7.33 to 15.23 microM and Heptanal concentrations from 2.47 to 9.23 microM were found in the lung cancer blood, while both hexanal and Heptanal in the control blood were lower than 0.6 microM. This further demonstrated that hexanal and Heptanal might be the biomarkers of lung cancer. The experimental results showed that GC-MS and HS-SPME with on-fiber derivatization is a simple, rapid, sensitive and solvent-free method for determination of in hexanal and Heptanal human blood.
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Investigation of volatile biomarkers in lung cancer blood using solid-phase microextraction and capillary gas chromatography-mass spectrometry.
Journal of Chromatography B, 2004Co-Authors: Chunhui Deng, Xiangmin Zhang, Ning LiAbstract:In the present work, solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) was developed for investigation of lung cancer volatile biomarkers. Headspace SPME conditions (fiber coating, extraction temperature and extraction time) and desorption conditions were optimized and applied to determination of volatiles in human blood. To find the biomarkers of lung cancer, investigation of volatile compounds in lung cancer blood and control was performed by using the present method. Concentrations of hexanal and Heptanal in lung cancer blood were found to be much higher than those in control blood. The two molecules of hexanal and Heptanal were regarded as biomarkers of lung cancer. By comparison of volatiles in breath and in blood, it is demonstrated that hexanal and Heptanal in breath were originated from blood and screening of lung cancer by breath analysis be feasible. These results show that SPME/GC-MS is a simple, rapid and sensitive method very suitable for investigation of volatile disease markers in human blood.
Chunhui Deng - One of the best experts on this subject based on the ideXlab platform.
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gas chromatography mass spectrometric analysis of hexanal and Heptanal in human blood by headspace single drop microextraction with droplet derivatization
Analytical Biochemistry, 2005Co-Authors: Ning Li, Chunhui Deng, Xizhong Shen, Xiangmin ZhangAbstract:Abstract In this work, we developed a new approach to the analysis of the lung cancer biomarkers, hexanal and Heptanal in human blood that was based on headspace single-drop microextraction (HS-SDME) with droplet derivatization, followed by gas chromatography–mass spectrometry (GC-MS). Aldehydes in blood were headspace extracted, concentrated, and derivatized by a suspended microdrop solvent containing the derivatization agent O -(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride. The aldehyde oximes formed in the microdrop solvent were analyzed by GC-MS. The optimal HS-SDME with droplet derivatization parameters extraction solvent of decane, sample temperature of 40 °C, extraction time of 6 min, stirring rate of 1100 rpm, and solvent volume of 2.0 μL were obtained and used for analysis of hexanal and Heptanal in blood. The method reproducibility, linearity, recovery, and detection limit were studied and the obtained results demonstrated the method feasibility. Finally, the proposed method was applied to the quantification of hexanal and Heptanal in cancer blood and normal blood. Due to sample extraction, concentration, and derivatization being performed in a single step, the method provided a simple, rapid, low-cost, and efficient approach to analysis of aldehydes in blood samples.
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determination of acetone hexanal and Heptanal in blood samples by derivatization with pentafluorobenzyl hydroxylamine followed by headspace single drop microextraction and gas chromatography mass spectrometry
Analytica Chimica Acta, 2005Co-Authors: Ning Li, Chunhui Deng, Xizhong Shen, Xiangmin ZhangAbstract:Abstract In the study, we developed a simple, rapid, sensitive, and inexpensive method for determination of the disease biomarkers of acetone, hexanal and Heptanal in human blood. For the first time, derivatization of carbonyls with O -2,3,4,5,6-(pentafluorobenzyl)hydroxylamine (PFBHA) was combined with headspace single-drop microextractin (HS-SDME) and gas chromatography-mass spectrometry (GC–MS) and applied to the analysis of acetone, hexanal, and Heptanal in human blood. At first, acetone, hexanal and Heptanal in blood were derivatized with PFBHA and formed oximes in several seconds. Sequentially, the oximes were headspace extracted and concentrated by a microdrop solvent. Finally, the extracted oximes were analyzed by GC–MS. HS-SDME conditions and method validations were studied. Due to needing of only 2 μl organic solvent, short extraction time of 8 min, and simple operation, derivatization-HS-SDME was shown to be a rapid, simple, and inexpensive technique for analysis of acetone, hexanal, and Heptanal in human blood. Moreover, it had low detection limit values from 0.24 to 0.62 nM, and good reproducibility (R.S.D. less than 12%).
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development of headspace solid phase microextraction with on fiber derivatization for determination of hexanal and Heptanal in human blood
Journal of Chromatography B, 2004Co-Authors: Chunhui Deng, Ning Li, Xiangmin ZhangAbstract:Abstract Hexanal and Heptanal in human blood have been regarded as potential biomarkers of lung cancer. Owing to their high volatilities and activities, it is difficult to accurately measure the two biomarkers. In the current work, headspace solid-phase microextraction (HS-SPME) with on-fiber derivatization technique was developed for quantitative analysis of hexanal and Heptanal in human blood. In the proposed method, the two aldehydes in blood were headspace extracted by using a poly (dimethylsiloxane)/divinylbenzene (PDMS/DVB) fiber with O-2,3,4,5,6-(pentafluorobenzyl) hydroxylamine (PFBHA) at 60 °C for 8 min. The aldehyde oximes formed on the fiber were desorbed and analyzed by gas chromatography–mass spectrometry (GC–MS). The method validations including detection limit, recovery and precision were studied. It was found that the method provided low detection limits of 0.006 nM for hexanal and 0.005 nM for Heptanal, recoveries from 89% to 95% and R.S.D. values less than 8.5%. The present method was applied to quantitative analysis of hexanal and Heptanal in normal blood and lung cancer blood. Hexanal concentrations from 7.33 to 15.23 μM and Heptanal concentrations from 2.47 to 9.23 μM were found in the lung cancer blood, while both hexanal and Heptanal in the control blood were lower than 0.6 μM. This further demonstrated that hexanal and Heptanal might be the biomarkers of lung cancer. The experimental results showed that GC–MS and HS-SPME with on-fiber derivatization is a simple, rapid, sensitive and solvent-free method for determination of in hexanal and Heptanal human blood.
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Development of headspace solid-phase microextraction with on-fiber derivatization for determination of hexanal and Heptanal in human blood.
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2004Co-Authors: Chunhui Deng, Ning Li, Xiangmin ZhangAbstract:Hexanal and Heptanal in human blood have been regarded as potential biomarkers of lung cancer. Owing to their high volatilities and activities, it is difficult to accurately measure the two biomarkers. In the current work, headspace solid-phase microextraction (HS-SPME) with on-fiber derivatization technique was developed for quantitative analysis of hexanal and Heptanal in human blood. In the proposed method, the two aldehydes in blood were headspace extracted by using a poly (dimethylsiloxane)/divinylbenzene (PDMS/DVB) fiber with O-2,3,4,5,6-(pentafluorobenzyl) hydroxylamine (PFBHA) at 60 degrees C for 8 min. The aldehyde oximes formed on the fiber were desorbed and analyzed by gas chromatography-mass spectrometry (GC-MS). The method validations including detection limit, recovery and precision were studied. It was found that the method provided low detection limits of 0.006 nM for hexanal and 0.005 nM for Heptanal, recoveries from 89% to 95% and R.S.D. values less than 8.5%. The present method was applied to quantitative analysis of hexanal and Heptanal in normal blood and lung cancer blood. Hexanal concentrations from 7.33 to 15.23 microM and Heptanal concentrations from 2.47 to 9.23 microM were found in the lung cancer blood, while both hexanal and Heptanal in the control blood were lower than 0.6 microM. This further demonstrated that hexanal and Heptanal might be the biomarkers of lung cancer. The experimental results showed that GC-MS and HS-SPME with on-fiber derivatization is a simple, rapid, sensitive and solvent-free method for determination of in hexanal and Heptanal human blood.
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Investigation of volatile biomarkers in lung cancer blood using solid-phase microextraction and capillary gas chromatography-mass spectrometry.
Journal of Chromatography B, 2004Co-Authors: Chunhui Deng, Xiangmin Zhang, Ning LiAbstract:In the present work, solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) was developed for investigation of lung cancer volatile biomarkers. Headspace SPME conditions (fiber coating, extraction temperature and extraction time) and desorption conditions were optimized and applied to determination of volatiles in human blood. To find the biomarkers of lung cancer, investigation of volatile compounds in lung cancer blood and control was performed by using the present method. Concentrations of hexanal and Heptanal in lung cancer blood were found to be much higher than those in control blood. The two molecules of hexanal and Heptanal were regarded as biomarkers of lung cancer. By comparison of volatiles in breath and in blood, it is demonstrated that hexanal and Heptanal in breath were originated from blood and screening of lung cancer by breath analysis be feasible. These results show that SPME/GC-MS is a simple, rapid and sensitive method very suitable for investigation of volatile disease markers in human blood.