The Experts below are selected from a list of 249789 Experts worldwide ranked by ideXlab platform

Xuemin Wang - One of the best experts on this subject based on the ideXlab platform.

Bart De Spiegeleer - One of the best experts on this subject based on the ideXlab platform.

  • peptide profiling of internet obtained cerebrolysin using high performance Liquid Chromatography electrospray ionization ion trap and ultra high performance Liquid Chromatography ion mobility quadrupole time of flight mass spectrometry
    Drug Testing and Analysis, 2015
    Co-Authors: Bert Gevaert, Matthias Dhondt, Nathalie Bracke, Han Yao, Evelien Wynendaele, Johannes Petrus Cornelis Vissers, Martin De Cecco, Jan Claereboudt, Bart De Spiegeleer
    Abstract:

    Cerebrolysin, a parenteral peptide preparation produced by controlled digestion of porcine brain proteins, is an approved nootropic medicine in some countries. However, it is also easily and globally available on the Internet. Nevertheless, until now, its exact chemical composition was unknown. Using high performance Liquid Chromatography (HPLC) coupled to ion trap and ultra high performance Liquid Chromatography (UHPLC) coupled to quadrupole-ion mobility-time-of-flight mass spectrometry (Q-IM-TOF MS), combined with UniProt pig protein database search and PEAKS de novo sequencing, we identified 638 unique peptides in an Internet-obtained Cerebrolysin sample. The main components in this sample originate from tubulin alpha- and beta-chain, actin, and myelin basic protein. No fragments of known neurotrophic factors like glial cell-derived neurotrophic factor (GDNF), neurotrophin nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF) were found, suggesting that the activities reported in the literature are likely the result of new, hitherto unknown cryptic peptides with nootropic properties.

Richard Dunphy - One of the best experts on this subject based on the ideXlab platform.

  • characterization of tepoxalin and its related compounds by high performance Liquid Chromatography mass spectrometry
    Journal of Pharmaceutical Sciences, 1994
    Co-Authors: David J. Burinsky, Barbara L. Armstrong, Alan R. Oyler, Richard Dunphy
    Abstract:

    High-Performance Liquid Chromatography/mass spectrometry (HPLC/MS) was used to identify and elucidate key structural characteristics of a novel dual cyclooxygenase/lipoxygenase inhibitor, tepoxalin (3-[5-(4-chlorophenyl)-1-(4-methoxyphenyl)-3-pyrazolyl]-N-hydroxy-N-methylpro-panamide), its synthesis-related byproducts in bulk drug substance, and degradation products stemming from probe stability studies. Such information is needed to assist in synthesis optimization and in the preparation of stable formulations for toxicity studies and clinical trials. High-Performance Liquid Chromatography, in conjunction with mass spectrometry (HPLC/MS) and UV photodiode array detection, can often expedite the gathering of preliminary data that contribute to several aspects of the pharmaceutical development process.

Jun Haginaka - One of the best experts on this subject based on the ideXlab platform.

  • protein based chiral stationary phases for high performance Liquid Chromatography enantioseparations
    Journal of Chromatography A, 2001
    Co-Authors: Jun Haginaka
    Abstract:

    The enantioseparations of various compounds using proteins as the chiral selectors in High-Performance Liquid Chromatography (HPLC) are considered in this review. The proteins used include albumins such as bovine serum albumin and human serum albumin, glycoproteins such as α1-acid glycoprotein, ovomucoid, ovoglycoprotein, avidin and riboflavin binding protein, enzymes such as trypsin, α-chymotrypsin, cellobiohydrolase I, lysozyme, pepsin and amyloglucosidase, and other proteins such as ovotransferrin and β-lactoglobulin. This review deals with the properties of HPLC chiral stationary phases based on proteins, and the enantioselective properties and chiral recognition mechanisms of these stationary phases.

Jean Bezard - One of the best experts on this subject based on the ideXlab platform.

  • separation of monoacylglycerols by reversed phase high performance Liquid Chromatography
    Journal of Chromatography A, 1992
    Co-Authors: B.g. Semporé, Jean Bezard
    Abstract:

    Abstract Reversed-phase High-Performance Liquid Chromatography on a thermostated octadecylsilyl column was used to separate complex mixtures of monoacyglycerols formed by chemical deacylation of natural triacyclycerols. Acetonitrile-water mixtures were used for elution of the underivatized monoacylglycerols and a different refractometer was used for their detection. The order of elution of monoacylglycerols in complex mixtures varies as a function of the chain length, unsaturation, positional isomerism, analysis temperature and eluent water concentration. The peak areas were representative of the amounts of monoacylglycerols detected. The method can be applied to compositional analysis and to the collection of non-contaminated fractions for further stereospecific analysis.