The Experts below are selected from a list of 1104057 Experts worldwide ranked by ideXlab platform

Miquel Costas - One of the best experts on this subject based on the ideXlab platform.

Jianguo Liu - One of the best experts on this subject based on the ideXlab platform.

  • Oriented Fermentation of Food Waste towards High-Value Products: A Review
    Energies, 2020
    Co-Authors: Qiao Wang, Feng Kai, Jianguo Liu
    Abstract:

    Food waste has a great potential for resource recovery due to its huge yield and high organic content. Oriented fermentation is a promising method with strong application prospects due to high efficiency, strong robustness, and High-Value Products. Different fermentation types lead to different Products, which can be shifted by adjusting fermentation conditions such as inoculum, pH, oxidation-reduction potential (ORP), organic loading rate (OLR), and nutrients. Compared with other types, lactic acid fermentation has the lowest reliance on artificial intervention. Lactic acid and volatile fatty acids are the common Products, and high yield and high purity are the main targets of food waste fermentation. In addition to operational parameters, reactors and processes should be paid more attention to for industrial application. Currently, continuously stirred tank reactors and one-stage processes are used principally for scale-up continuous fermentation of food waste. Electro-fermentation and iron-based or carbon-based additives can improve food waste fermentation, but their mechanisms and application need further investigation. After fermentation, the recovery of target Products is a key problem due to the lack of green and economic methods. Precipitation, distillation, extraction, adsorption, and membrane separation can be considered, but the recovery step is still the most expensive in the entire treatment chain. It is expected to develop more efficient fermentation processes and recovery strategies based on food waste composition and market demand.

Carl W. Lawton - One of the best experts on this subject based on the ideXlab platform.

  • Cellular and metabolic engineering of oleaginous yeast Yarrowia lipolytica for bioconversion of hydrophobic substrates into High-Value Products.
    Engineering in Life Sciences, 2019
    Co-Authors: Ya-hue Valerie Soong, Seongkyu Yoon, Carl W. Lawton
    Abstract:

    The non-conventional oleaginous yeast Yarrowia lipolytica is able to utilize both hydrophilic and hydrophobic carbon sources as substrates and convert them into value-added bioProducts such as organic acids, extracellular proteins, wax esters, long-chain diacids, fatty acid ethyl esters, carotenoids and omega-3 fatty acids. Metabolic pathway analysis and previous research results show that hydrophobic substrates are potentially more preferred by Y. lipolytica than hydrophilic substrates to make High-Value Products at higher productivity, titer, rate, and yield. Hence, Y. lipolytica is becoming an efficient and promising biomanufacturing platform due to its capabilities in biosynthesis of extracellular lipases and directly converting the extracellular triacylglycerol oils and fats into High-Value Products. It is believed that the cell size and morphology of the Y. lipolytica is related to the cell growth, nutrient uptake, and product formation. Dimorphic Y. lipolytica demonstrates the yeast-to-hypha transition in response to the extracellular environments and genetic background. Yeast-to-hyphal transition regulating genes, such as YlBEM1, YlMHY1 and YlZNC1 and so forth, have been identified to involve as major transcriptional factors that control morphology transition in Y. lipolytica. The connection of the cell polarization including cell cycle and the dimorphic transition with the cell size and morphology in Y. lipolytica adapting to new growth are reviewed and discussed. This review also summarizes the general and advanced genetic tools that are used to build a Y. lipolytica biomanufacturing platform.

Birger Lindberg Møller - One of the best experts on this subject based on the ideXlab platform.

  • Chapter 12:Disruptive innovation: channeling photosynthetic electron flow into light-driven synthesis of High-Value Products
    Synthetic Biology, 2014
    Co-Authors: Birger Lindberg Møller
    Abstract:

    In living cells, the formation of enzyme complexes plays a key role in controlling the channeling of metabolic fluxes towards specific targets. We aim to use synthetic biology to construct enzyme complexes mimicking those in nature but with new catalytic properties. This review offers an account of some of the current synthetic biology approaches undertaken based on the “share-your-parts” principle to engineer chloroplasts into light-driven effective production units for high value bio-active natural Products such as pharmaceutically important structurally complex diterpenoids. The synthesis of most bio-active natural Products involves biosynthetic steps catalysed by cytochrome P450 enzymes. Re-routing of such biosynthetic pathways into the chloroplast is possible as demonstrated with the pathway for the cyanogenic glucoside dhurrin. This constitutes an ideal proof-of-concept model system because all the genes and enzymes involved are well characterized. Photosystem I is the key and unique bio-brick used in these synthetic biology efforts with ferredoxin constituting an eminent electron donor of reducing equivalents to cytochrome P450s anchored in the thylakoid membrane of the chloroplast. Approaches to further optimize and channel the use of reducing power from photosystem I into the production of bio-active natural Products based on construction of a supramolecular metabolon composed of photosystem I into which the catalytic globular domains of cytochrome P450s have been incorporated are presented and discussed. The chloroplast is an ideal production unit for diterpenoids because it is the site of synthesis of the universal precursor geranylgeranyl diphosphate. The technologies available and advances made in identifying and characterizing the genes and enzymes involved in the biosynthesis of complex diterpenoids are outlined and illustrated with the “silver bullet drug” forskolin as an example. More than 50.000 terpenoids have been detected in plants, making terpenoids the richest repository for chemicals with a wide range of bioactivities. An ultimate goal of the use of synthetic biology within this research area is to design a “plug-and-play” template-based production system that on a long-term basis can contribute to production of otherwise extremely costly medicinal compounds. This review also serves to illustrate how utilization of solar energy in combination with the approaches of synthetic biology may possibly help us to provide science based solutions to some of the global challenges we are facing and be a driver in our efforts to move towards a bio-based society.

Henrique A. Matos - One of the best experts on this subject based on the ideXlab platform.

  • Extraction of high value Products from avocado waste biomass
    The Journal of Supercritical Fluids, 2020
    Co-Authors: Patrícia R.s. Páramos, José F.o. Granjo, Marcos L. Corazza, Henrique A. Matos
    Abstract:

    Abstract This work studies the oil extraction and recovery of bioactive compounds from Brazilian and Mexican avocado seeds and peels of Hass cultivar using Soxhlet (SE) with hexane, ethanol, and ethyl acetate as solvents; and supercritical carbon dioxide (scCO2) with ethanol and ethyl acetate as cosolvents. Also, the fatty acids (FAs) profile, total phenolic content (TPC), and antioxidant activity (AA) in avocado seeds measured. Finally, the dynamic extraction curves of scCO2 were modeled with a multi-way partial least squares regression (MPLSR). The highest extraction yields were observed when ethanol is used as solvent in SE (up to 14 wt%) and as cosolvent in scCO2 (up to 6.9 wt%). TPC was higher in SFE of avocado seeds, while higher values of AA were obtained using SE. MPLSR model obtained for the scCO2 extraction showed an average absolute deviation below 0.5 wt% for 360 measurements using only eight factors.