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Ya-ting Liu - One of the best experts on this subject based on the ideXlab platform.
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identification of taeniothrips eucharii thysanoptera thripidae as a vector of Hippeastrum chlorotic ringspot virus in southern china
Plant Disease, 2017Co-Authors: Xue Gao, Zhiqiang Jia, Ya-ting LiuAbstract:Hippeastrum chlorotic ringspot virus (HCRV) is a putative new member of the genus Tospovirus, which was first identified infecting Hymenocallis littoralis in Yunnan Province, southwestern China. Taeniothrips eucharii, the dominant thrips species found on H. littoralis, was tested for its efficiency as a vector of the virus. We used Western blot analysis of adult thrips to detect the N protein. Transmission experiments demonstrated an average acquisition efficiency of 43.7 ± 3.4% (AAP = 24 h) for the first larval stage and a transmission efficiency of 19.1 ± 2.4% (IAP = 24 h) for adult thrips. This study reports T. eucharii as a new genus and species of thrips transmitting a Tospovirus, HCRV.
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Molecular characterization of the Hippeastrum chlorotic ringspot virus L segment and its protein
Archives of virology, 2014Co-Authors: Shi-gao Lou, Li-peng Sun, Ya-ting LiuAbstract:Hippeastrum chlorotic ringspot virus (HCRV) is a novel tospovirus that was identified in Yunnan Province, China, in 2013. We have sequenced the HCRV L gene, which is 8909 nt long and encodes the RNA-dependent RNA polymerase (2873 amino acids, 330.8 kDa). The HCRV L protein shared highest similarity (89.4 %) with that of tomato yellow ring virus. The L protein contains a negative-sense RNA virus RNA-directed RNA polymerase motif and an endonuclease domain at the N-terminus. Combined with our previous reports of the S and M RNAs, the genome sequence of HCRV is now completed.
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the complete s rna and m rna nucleotide sequences of a Hippeastrum chlorotic ringspot virus hcrv isolate from hymenocallis littoralis jacq salisb in china
Archives of Virology, 2013Co-Authors: Shi-gao Lou, Yuanxian Zheng, Wencheng Wang, Ya-ting LiuAbstract:An isolate of Hippeastrum chlorotic ringspot virus (HCRV) named HLS1-2, causing necrotic spots on leaves of spider lily, was obtained and characterized in China. The complete S RNA and M RNAs of the HLS1-2 isolate are 2724 nt and 4741 nt in length, respectively. The HLS1-2 S RNA sequence is most closely related to that of HCRV, with 99 % identity. Viral proteins encoded by the M RNA are closely related to those of tomato yellow ring virus (TYRV), polygonum ringspot virus (PolRSV) and iris yellow spot virus (IYSV). Phylogenetic trees for the four viral proteins encoded by the S and M RNAs placed HCRV-HLS1-2 in a distinct cluster with IYSV, TYRV and PolRSV and provided further support for the subdivision of tospoviruses into American and Eurasian groups.
Jaume Bastida - One of the best experts on this subject based on the ideXlab platform.
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Glycosylated narciclasine alkaloid in Hippeastrum puniceum (Lam.) Kuntze
South African Journal of Botany, 2020Co-Authors: Amanda Eiriz Feu, Jaume Bastida, Jean Paulo De Andrade, Alejandro Ayala, Larissa Costa De Almeida, Letícia V. Costa-lotufo, Javier Ellena, Warley De Souza BorgesAbstract:Abstract The present work focused on the chemical study of the main alkaloid component in Brazilian indigenous Amaryllidaceae species Hippeastrum puniceum (Lam.) Kuntze. The strategy applied was based on a distinct partitioning process and chromatographic-purification steps using the crude methanolic extract as the starting material. In this way, the glycosylated derivative narciclasine-4-O-β-D-xylopyranoside was isolated from the ethyl acetate fraction of H. puniceum. Structural elucidation of the compound was performed through NMR (Nuclear Magnetic Resonance) analysis including mono- and bi-dimensional experiments. The absolute configuration was determined herein for the first time by means of X-Ray Crystallography. The compound was also evaluated as cytotoxic agent against colon (HCT 116) and breast (MCF-7) tumor cells, showing no significant activity at the tested concentrations of 10 and 50 μM.
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Hippeastrum reticulatum amaryllidaceae alkaloid profiling biological activities and molecular docking
Molecules, 2017Co-Authors: Luciana R Tallini, Warley De Souza Borges, Edison Osorio, Vanessa Dias Dos Santos, Marcel Kaiser, Jose Angelo S Zuanazzi, Jaume BastidaAbstract:The Amaryllidaceae family has proven to be a rich source of active compounds, which are characterized by unique skeleton arrangements and a broad spectrum of biological activities. The aim of this work was to perform the first detailed study of the alkaloid constituents of Hippeastrum reticulatum (Amaryllidaceae) and to determine the anti-parasitological and cholinesterase (AChE and BuChE) inhibitory activities of the epimers (6α-hydroxymaritidine and 6β-hydroxymaritidine). Twelve alkaloids were identified in H. reticulatum: eight known alkaloids by GC-MS and four unknown (6α-hydroxymaritidine, 6β-hydroxymaritidine, reticulinine and isoreticulinine) by NMR. The epimer mixture (6α-hydroxymaritidine and 6β-hydroxymaritidine) showed low activity against all protozoan parasites tested and weak AChE-inhibitory activity. Finally, a molecular docking analysis of AChE and BuChE proteins showed that isoreticulinine may be classified as a potential inhibitory molecule since it can be stabilized in the active site through hydrogen bonds, π-π stacking and hydrophobic interactions.
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alkaloids from Hippeastrum argentinum and their cholinesterase inhibitory activities an in vitro and in silico study
Journal of Natural Products, 2016Co-Authors: Javier E Ortiz, Jaume Bastida, Natalia B Pigni, Sebastian A Andujar, German Roitman, Fernando D Suvire, Ricardo D Enriz, Alejandro Tapia, Gabriela Egly FeresinAbstract:Two new alkaloids, 4-O-methylnangustine (1) and 7-hydroxyclivonine (2) (montanine and homolycorine types, respectively), and four known alkaloids were isolated from the bulbs of Hippeastrum argentinum, and their cholinesterase-inhibitory activities were evaluated. These compounds were identified using GC-MS, and their structures were defined by physical data analysis. Compound 2 showed weak butyrylcholinesterase (BuChE)-inhibitory activity, with a half-maximal inhibitory concentration (IC50) value of 67.3 ± 0.09 μM. To better understand the experimental results, a molecular modeling study was also performed. The combination of a docking study, molecular dynamics simulations, and quantum theory of atoms in molecules calculations provides new insight into the molecular interactions of compound 2 with BuChE, which were compared to those of galantamine.
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Identification of Alkaloids from Hippeastrum aulicum (Ker Gawl.) Herb. (Amaryllidaceae) Using CGC-MS and Ambient Ionization Mass Spectrometry (PS-MS and LS-MS)
Journal of the Brazilian Chemical Society, 2016Co-Authors: Carliani Dal Piero B. Bessa, Jaume Bastida, Jean Paulo De Andrade, Renata Biella Salles De Oliveira, Eloilson Domingos, Heloa Santos, Wanderson Romão, Warley De Souza BorgesAbstract:Amaryllidaceae alkaloids are well-known isoquinolines which have demonstrated a wide range of biological activities such as antiviral, anticancer, acetylcholinesterase inhibition, antimalarial, among others. Mass spectrometry (MS) studies based on capillary gas chromatography (CGC), paper spray (PS), and leaf spray (LS) ionization were carried out for alkaloid investigation of the native Brazilian species Hippeastrum aulicum, along with nuclear magnetic resonance (NMR) techniques. Thirty-one alkaloids were identified including the new compound haemanthamine N-oxide. The results from PS- and LS-MS techniques were consistent with those observed in CGC-MS analysis. To the best of our knowledge, it is the first study combining NMR, CGC-MS and the ambient ionization-mass spectrometry (PS- and LS-MS) on Amaryllidaceae plants.
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New lycosinine derivative from Hippeastrum breviflorum
Revista Brasileira de Farmacognosia, 2015Co-Authors: Camila Sebben, Strahil Berkov, Jaume Bastida, Jean Paulo De Andrade, Raquel Brandt Giordani, Edison Javier Osorio, Marcos Sobral, Mauro Vieira De Almeida, Amélia Teresinha Henriques, Jose Angelo S ZuanazziAbstract:A new lycosinine derivative, 9-O-demethyllycosinine B, was isolated from the native Brazilian Hippeastrum breviflorum Herb., Amaryllidaceae, along with the well-known alkaloids lycosinine B and lycorine. The structure of the new compound was established by physical and spectroscopic methods. 9-Odemethyllycosinine B is the third lycosinine variant identified in the Amaryllidaceae family.
Werner Döpke - One of the best experts on this subject based on the ideXlab platform.
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Two novel Amaryllidaceae alkaloids from Hippeastrum equestre Herb.: 3-O-demethyltazettine and egonine
Phytochemistry, 1999Co-Authors: Lam Huong Pham, Egon Gründemann, Michael Bartoszek, Jürgen Wagner, Werner DöpkeAbstract:Abstract From the bulbs of vietnamese Hippeastrum equestre Herb. (Amaryllidaceae), two novel Amaryllidaceae alkaloids 3- O -demethyltazettine and egonine have been isolated. Their structures were established by spectroscopic analysis (UV, MS, NMR and CD) as well as by comparison with respective spectroscopic data of tazettine and tazettadiol.
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Alkaloids from Hippeastrum equestre Herb. (Amaryllidaceae). Part III
Pharmazie, 1997Co-Authors: Lam Huong Pham, Egon Gründemann, Werner DöpkeAbstract:From the bulbs of vietnamese Hippeastrum equestre Herb. (Amaryllidaceae), ismine (1), 11-hydroxyvittatine (2), 9-O-demethylhomolycorine (3), pretazettine (4), and 3-epimacronine (5) were isolated for the first time. Their structures were established by spectroscopic methods
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alkaloids from Hippeastrum equestre herb 5 circular dichroism studies
Tetrahedron, 1996Co-Authors: Jürgen Wagner, Huong L Pham, Werner DöpkeAbstract:Abstract A survey of the CD spectra of eight Amaryllidaceae alkaloids (3, 5, 7, 8, 11, 12, 13, 14) isolated from Hippeastrum equestre Herb. and six Amaryllidaceae alkaloids (1, 2, 4, 6, 9, 10) of related structure indicates that this technique can provide useful structural information regarding the basic heterocyclic ring system and the stereochemistry of the dominant ring junction, respectively.
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alkaloids from Hippeastrum equestre part i phamine a new phenanthridone alkaloid
Planta Medica, 1995Co-Authors: Werner Döpke, Lam Huong Pham, Egon Gründemann, Michael Bartoszek, Sabine FlatauAbstract:From the bulbs of Vietnamese Hippeastrum equestre Herb. (Amaryllidaceae), besides the well known alkaloids lycorine, tazettine, and hippeastrine, a new alkaloid, phamine, has been isolated. Its structure was established by spectroscopic methods.
Annemieke Vandamme - One of the best experts on this subject based on the ideXlab platform.
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profile of resistance of human immunodeficiency virus to mannose specific plant lectins
Journal of Virology, 2004Co-Authors: Annemieke Vandamme, Kurt Vermeire, Sigrid Hatse, Els J. M. Van Damme, Willy J Peumans, Kristel Van LaethemAbstract:The mannose-specific plant lectins from the Amaryllidaceae family (e.g., Hippeastrum sp. hybrid and Galanthus nivalis) inhibit human immunodeficiency virus (HIV) infection of human lymphocytic cells in the higher nanogram per milliliter range and suppress syncytium formation between persistently HIV type 1 (HIV-1)-infected cells and uninfected CD4+ T cells. These lectins inhibit virus entry. When exposed to escalating concentrations of G. nivalis and Hippeastrum sp. hybrid agglutinin, a variety of HIV-1(IIIB) strains were isolated after 20 to 40 subcultivations which showed a decreased sensitivity to the plant lectins. Several amino acid changes in the envelope glycoprotein gp120, but not in gp41, of the mutant virus isolates were observed. The vast majority of the amino acid changes occurred at the N glycosylation sites and at the S or T residues that are part of the N glycosylation motif. The degree of resistance to the plant lectins was invariably correlated with an increasing number of mutated glycosylation sites in gp120. The nature of these mutations was entirely different from that of mutations that are known to appear in HIV-1 gp120 under the pressure of other viral entry inhibitors such as dextran sulfate, bicyclams (i.e., AMD3100), and chicoric acid, which also explains the lack of cross-resistance of plant lectin-resistant viruses to any other HIV inhibitor including T-20 and the blue-green algae (cyanobacteria)-derived mannose-specific cyanovirin. The plant lectins represent a well-defined class of anti-HIV (microbicidal) drugs with a novel HIV drug resistance profile different from those of other existing anti-HIV drugs.
Jean Paulo De Andrade - One of the best experts on this subject based on the ideXlab platform.
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Glycosylated narciclasine alkaloid in Hippeastrum puniceum (Lam.) Kuntze
South African Journal of Botany, 2020Co-Authors: Amanda Eiriz Feu, Jaume Bastida, Jean Paulo De Andrade, Alejandro Ayala, Larissa Costa De Almeida, Letícia V. Costa-lotufo, Javier Ellena, Warley De Souza BorgesAbstract:Abstract The present work focused on the chemical study of the main alkaloid component in Brazilian indigenous Amaryllidaceae species Hippeastrum puniceum (Lam.) Kuntze. The strategy applied was based on a distinct partitioning process and chromatographic-purification steps using the crude methanolic extract as the starting material. In this way, the glycosylated derivative narciclasine-4-O-β-D-xylopyranoside was isolated from the ethyl acetate fraction of H. puniceum. Structural elucidation of the compound was performed through NMR (Nuclear Magnetic Resonance) analysis including mono- and bi-dimensional experiments. The absolute configuration was determined herein for the first time by means of X-Ray Crystallography. The compound was also evaluated as cytotoxic agent against colon (HCT 116) and breast (MCF-7) tumor cells, showing no significant activity at the tested concentrations of 10 and 50 μM.
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Identification of Alkaloids from Hippeastrum aulicum (Ker Gawl.) Herb. (Amaryllidaceae) Using CGC-MS and Ambient Ionization Mass Spectrometry (PS-MS and LS-MS)
Journal of the Brazilian Chemical Society, 2016Co-Authors: Carliani Dal Piero B. Bessa, Jaume Bastida, Jean Paulo De Andrade, Renata Biella Salles De Oliveira, Eloilson Domingos, Heloa Santos, Wanderson Romão, Warley De Souza BorgesAbstract:Amaryllidaceae alkaloids are well-known isoquinolines which have demonstrated a wide range of biological activities such as antiviral, anticancer, acetylcholinesterase inhibition, antimalarial, among others. Mass spectrometry (MS) studies based on capillary gas chromatography (CGC), paper spray (PS), and leaf spray (LS) ionization were carried out for alkaloid investigation of the native Brazilian species Hippeastrum aulicum, along with nuclear magnetic resonance (NMR) techniques. Thirty-one alkaloids were identified including the new compound haemanthamine N-oxide. The results from PS- and LS-MS techniques were consistent with those observed in CGC-MS analysis. To the best of our knowledge, it is the first study combining NMR, CGC-MS and the ambient ionization-mass spectrometry (PS- and LS-MS) on Amaryllidaceae plants.
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New lycosinine derivative from Hippeastrum breviflorum
Revista Brasileira de Farmacognosia, 2015Co-Authors: Camila Sebben, Strahil Berkov, Jaume Bastida, Jean Paulo De Andrade, Raquel Brandt Giordani, Edison Javier Osorio, Marcos Sobral, Mauro Vieira De Almeida, Amélia Teresinha Henriques, Jose Angelo S ZuanazziAbstract:A new lycosinine derivative, 9-O-demethyllycosinine B, was isolated from the native Brazilian Hippeastrum breviflorum Herb., Amaryllidaceae, along with the well-known alkaloids lycosinine B and lycorine. The structure of the new compound was established by physical and spectroscopic methods. 9-Odemethyllycosinine B is the third lycosinine variant identified in the Amaryllidaceae family.
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Crinine-type alkaloids from Hippeastrum aulicum and H. calyptratum
Phytochemistry, 2014Co-Authors: Jean Paulo De Andrade, Francesc Viladomat, Carles Codina, Ying Guo, Mercè Font-bardia, Teresa Calvet, Jullie Dutilh, Jose Angelo S ZuanazziAbstract:An ongoing search for alkaloids in the Amaryllidaceae species using GC–MS resulted in the identification of two crinine-type alkaloids, aulicine (1) and 3-O-methyl-epimacowine, (2) from the indigenous Brazilian species Hippeastrum aulicum and Hippeastrum calyptratum, respectively. In addition, two alkaloids, 11-oxohaemanthamine (3) and 7-methoxy-O-methyllycorenine (4) were both isolated from H. aulicum. Furthermore, we provide here complete NMR spectroscopic data for the homolycorine analogues nerinine (5) and albomaculine (6). The absolute stereochemistry of the 5,10b-ethano bridge in the crinine variants was determined by circular dichroism and X-ray crystallographic analysis, thus presenting the first direct evidence for the presence of crinine-type alkaloids in the genus Hippeastrum.
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alkaloids from the Hippeastrum genus chemistry and biological activity
Revista Latinoamericana de Química, 2012Co-Authors: Jean Paulo De Andrade, Strahil Berkov, Carles Codina, Ying Guo, Jose Angelo S Zuanazzi, Natalia B Pigni, Laura Torrasclaveria, Ricardo Reyeschilpa, Abdelaziz El Amrani, Francesc ViladomatAbstract:In recent years alkaloids from the genus Hippeastrum have been shown to exhibit a broad spectrum of biological activities, including antiparasitic, antiproliferative, apoptosis-induced, psychopharmacological, acetylcholinesterase-inhibitory, among others. This work presents a brief chemical and biological review of the alkaloids found in the genus Hippeastrum. www.relaquim.com