The Experts below are selected from a list of 5022 Experts worldwide ranked by ideXlab platform
Young Ho Kim - One of the best experts on this subject based on the ideXlab platform.
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Two New Triterpene Glycosides from the Vietnamese Sea CucumberHolothuria scabra
Archives of Pharmacal Research, 2007Co-Authors: Nguyen Hai Dang, Nguyen Thanh, Phan Kiem, Le Mai Huong, Chau Minh, Young Ho KimAbstract:Two new triterpene glycosides, namely holothurin A_3 ( 1 ) and A_4 ( 2 ) were isolated from the methanol extract of the sea cucumber, Holothuria scabra , with their structures elucidated from the spectroscopic evidence (1D NMR, 2D NMR, ESI-MS and HRESI-MS). Compounds 1 and 2 were found to be strongly cytotoxic to both cancer cell lines, KB and Hep-G2, with 50% inhibitory concentrations (IC_50) of 0.87 and 0.32 μg/mL (for compound 1) and of 1.12 and 0.57 μg/mL (for compound 2 ), respectively.
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two new triterpene glycosides from the vietnamese sea cucumber Holothuria scabra
Archives of Pharmacal Research, 2007Co-Authors: Nguyen Hai Dang, Le Mai Huong, Nguyen Van Thanh, Phan Van Kiem, Chau Van Minh, Young Ho KimAbstract:Two new triterpene glycosides, namely holothurin A3 (1) and A4 (2) were isolated from the methanol extract of the sea cucumber, Holothuria scabra, with their structures elucidated from the spectroscopic evidence (1D NMR, 2D NMR, ESI-MS and HRESI-MS). Compounds 1 and 2 were found to be strongly cytotoxic to both cancer cell lines, KB and Hep-G2, with 50% inhibitory concentrations (IC50) of 0.87 and 0.32 microg/mL (for compound 1) and of 1.12 and 0.57 microg/mL (for compound 2), respectively.
Pavel S. Dmitrenok - One of the best experts on this subject based on the ideXlab platform.
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Triterpene glycosides from the Vietnamese sea cucumber Holothuria edulis
Natural product research, 2019Co-Authors: Le Hoang, Vien Le Thi, Hanh Tran Thi Hong, Thanh Nguyen Van, Cuong Nguyen Xuan, Nam Nguyen Hoai, Thung Do Cong, Natalia V. Ivanchina, Thao Do Thi, Pavel S. DmitrenokAbstract:From the MeOH extract of the Vietnamese sea cucumber Holothuria edulis, eight triterpene glycosides (1–8), including one new compound namely holothurin A5 (1), were isolated by using various chroma...
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Triterpene glycosides from the Vietnamese sea cucumber Holothuria edulis
2019Co-Authors: Le Hoang, Hanh Tran Thi Hong, Thanh Nguyen Van, Cuong Nguyen Xuan, Nam Nguyen Hoai, Thung Do Cong, Natalia V. Ivanchina, Thao Do Thi, Vien Le Thi, Pavel S. DmitrenokAbstract:From the MeOH extract of the Vietnamese sea cucumber Holothuria edulis, eight triterpene glycosides (1–8), including one new compound namely holothurin A5 (1), were isolated by using various chromatographic separations. Their structures were established by spectroscopic experiments including 1D, 2D NMR and HR-ESI-MS. Holothurin A5 (1) has a hydroperoxy group at C-25. To the best of our knowledge, this is the first report of this group in triterpene saponins obtained from sea cucumbers to date. In addition, the in vitro cytotoxicity against five human cancer cell lines (HepG2, KB, LNCaP, MCF7 and SK-Mel2) of all isolated compounds was also evaluated using SRB assays.
Nguyen Hai Dang - One of the best experts on this subject based on the ideXlab platform.
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Two New Triterpene Glycosides from the Vietnamese Sea CucumberHolothuria scabra
Archives of Pharmacal Research, 2007Co-Authors: Nguyen Hai Dang, Nguyen Thanh, Phan Kiem, Le Mai Huong, Chau Minh, Young Ho KimAbstract:Two new triterpene glycosides, namely holothurin A_3 ( 1 ) and A_4 ( 2 ) were isolated from the methanol extract of the sea cucumber, Holothuria scabra , with their structures elucidated from the spectroscopic evidence (1D NMR, 2D NMR, ESI-MS and HRESI-MS). Compounds 1 and 2 were found to be strongly cytotoxic to both cancer cell lines, KB and Hep-G2, with 50% inhibitory concentrations (IC_50) of 0.87 and 0.32 μg/mL (for compound 1) and of 1.12 and 0.57 μg/mL (for compound 2 ), respectively.
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two new triterpene glycosides from the vietnamese sea cucumber Holothuria scabra
Archives of Pharmacal Research, 2007Co-Authors: Nguyen Hai Dang, Le Mai Huong, Nguyen Van Thanh, Phan Van Kiem, Chau Van Minh, Young Ho KimAbstract:Two new triterpene glycosides, namely holothurin A3 (1) and A4 (2) were isolated from the methanol extract of the sea cucumber, Holothuria scabra, with their structures elucidated from the spectroscopic evidence (1D NMR, 2D NMR, ESI-MS and HRESI-MS). Compounds 1 and 2 were found to be strongly cytotoxic to both cancer cell lines, KB and Hep-G2, with 50% inhibitory concentrations (IC50) of 0.87 and 0.32 microg/mL (for compound 1) and of 1.12 and 0.57 microg/mL (for compound 2), respectively.
Mercedes Gonzalezwanguemert - One of the best experts on this subject based on the ideXlab platform.
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molecular systematics of the genus Holothuria in the mediterranean and northeastern atlantic and a molecular clock for the diversification of the holothuriidae echinodermata holothuroidea
Molecular Phylogenetics and Evolution, 2010Co-Authors: Giomar Helena Borreroperez, Concepcion Marcos, Mercedes Gonzalezwanguemert, Jesus Gomezzurita, Angel PerezruzafaAbstract:This work investigates the systematics of the genus Holothuria in the Mediterranean Sea and Northeastern Atlantic in the light of a wider molecular phylogenetic hypothesis of Holothuriidae, and it also provides a time-scale for the family diversification using mitochondrial markers and the molecular clock hypothesis. The subgenera Holothuria and Roweothuria are retrieved as paraphyletic. At least four separate lineages, with quite different time frameworks were identified. There are at least three species with an apparent long evolutionary history, H. forskali, H. sanctori and H. impatiens and six species belonging to Holothuria, Roweothuria and Vaneyothuria, which have diverged relatively recently.
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the taxonomic status of some atlanto mediterranean species in the subgenus Holothuria echinodermata holothuroidea holothuriidae based on molecular evidence
Zoological Journal of the Linnean Society, 2009Co-Authors: Giomar Helena Borreroperez, Angel Perezruzafa, Concepcion Marcos, Mercedes GonzalezwanguemertAbstract:Molecular and morphological data were used to evaluate the taxonomic status of the species Holothuria tubulosa Gmelin, 1790, Holothuria stellati Delle Chiaje, 1823, Holothuria mammata Grube, 1840, and Holothuria dakarensis Panning, 1939, belonging to the nominate subgenus Holothuria (Holothuria) (family Holothuriidae) from the Mediterranean Sea and Atlantic Ocean. A 16S rRNA marker distinguished three well-supported clades with clear genetic differentiation amongst them. The morphometric characters, although they reflected the clades, showed great variability, and some specimens from different clades overlapped. The morphological data and the literature suggest that the clades correspond to H. dakarensis (from Cape Verde Islands), H. mammata (from the AtlantoMediterranean area) and H. tubulosa (from the Mediterranean Sea). Holothuria stellati is considered here to be a junior subjective synonym of H. tubulosa. Great morphological intraspecific variation within H. tubulosa and H. mammata explains the confusion in the literature. Holothuria tubulosa includes specimens with distinctive ossicles, but others are similar to H. mammata. In these cases, the presence or absence of Cuvierian tubules proved a reliable indicator to the identity of these species; unfortunately this character is difficult to assess in preserved material. According to the results of discriminant analysis we propose a set of ossicle morphometric variables that permit the optimum assignation of individuals to the clades. Our results present a new perspective on the taxonomic status of species in Holothuria (Holothuria), and show how a molecular approach, combined with a morphological approach, can solve taxonomic problems.
Isabel Reche - One of the best experts on this subject based on the ideXlab platform.
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Antibacterial activity of the 27 isolated bacteria from Anemonia sulcata (samples M1, M2), Holothuria tubulosa (M3, M4), Holothuria forskali (M6), Actinia equina (M7, M8) and Holothurian feces (M9) against eight pathogenic bacteria.
2018Co-Authors: Elizabeth León-palmero, Vanessa Joglar, Pedro A. Álvarez, Antonio Martín-platero, Inmaculada Llamas, Isabel RecheAbstract:Antibacterial activity of the 27 isolated bacteria from Anemonia sulcata (samples M1, M2), Holothuria tubulosa (M3, M4), Holothuria forskali (M6), Actinia equina (M7, M8) and Holothurian feces (M9) against eight pathogenic bacteria.
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Antifungal activity of the 12 selected bacteria from Anemonia sulcata (samples M1, M2), Holothuria tubulosa (M3, M4), Actinia equina (M8) and Holothurian feces (M9) against the nine indicator fungi.
2018Co-Authors: Elizabeth León-palmero, Vanessa Joglar, Pedro A. Álvarez, Antonio Martín-platero, Inmaculada Llamas, Isabel RecheAbstract:Antifungal activity of the 12 selected bacteria from Anemonia sulcata (samples M1, M2), Holothuria tubulosa (M3, M4), Actinia equina (M8) and Holothurian feces (M9) against the nine indicator fungi.
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Diversity and antimicrobial potential in sea anemone and Holothurian microbiomes
2018Co-Authors: Elizabeth León-palmero, Vanessa Joglar, Pedro A. Álvarez, Antonio Martín-platero, Inmaculada Llamas, Isabel RecheAbstract:Marine invertebrates, as holobionts, contain symbiotic bacteria that coevolve and develop antimicrobial substances. These symbiotic bacteria are an underexplored source of new bioactive molecules to face the emerging antibiotic resistance in pathogens. Here, we explored the antimicrobial activity of bacteria retrieved from the microbiota of two sea anemones (Anemonia sulcata, Actinia equina) and two Holothurians (Holothuria tubulosa, Holothuria forskali). We tested the antimicrobial activity of the isolated bacteria against pathogens with interest for human health, agriculture and aquaculture. We isolated 27 strains with antibacterial activity and 12 of these isolates also showed antifungal activity. We taxonomically identified these strains being Bacillus and Vibrio species the most representative producers of antimicrobial substances. Microbiome species composition of the two sea anemones was similar between them but differed substantially of seawater bacteria. In contrast, microbiome species composition of the two Holothurian species was different between them and in comparison with the bacteria in Holothurian feces and seawater. In all the holobiont microbiomes Bacteroidetes was the predominant phylum. For each microbiome, we determined diversity and the rank-abundance dominance using five fitted models (null, pre-emption, log-Normal, Zipf and Zipf-Mandelbrot). The models with less evenness (i.e. Zipf and Zipf-Mandelblot) showed the best fits in all the microbiomes. Finally, we tracked (using the V4 hypervariable region of 16S rRNA gene) the relative abundance of these 27 isolates with antibacterial activity in the total pool of sequences obtained for the microbiome of each holobiont. Coincidences, although with extremely low frequencies, were detected only in the microbiome of H. forskali. This fact suggests that these isolated bacteria belong to the long tail of rare symbiotic bacteria. Therefore, more and more sophisticated culture techniques are necessary to explore this apparently vast pool of rare symbiontic bacteria and to determine their biotechnological potentiality.