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Guillermo Giovambattista - One of the best experts on this subject based on the ideXlab platform.
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Association between BoLA-DRB3 and somatic cell count in Holstein Cattle from Argentina
Molecular Biology Reports, 2012Co-Authors: Laura Rosana Baltian, María Verónica Ripoli, S. Sanfilippo, S. N. Takeshima, Y. Aida, Guillermo GiovambattistaAbstract:Different studies have proved that the resistance/susceptibility to mastitis is genetically determined. The major histocompatibility complex in cows is known as bovine lymphocyte antigen (BoLA). Genes from the BoLA have been associated with the occurrence of infectious diseases such as mastitis and leukosis, especially the BoLA-DRB gene. The object of the present study was to detect associations between BoLA-DRB3 alleles and somatic cell count (SCC), as an indicator of resistance/susceptibility to mastitis in Holstein Cattle ( N = 123) from La Pampa, Argentina. Fisher’s exact test and Woolf–Haldane odds ratio were applied to study the association between SCC and BoLA-DRB3 allele frequencies. Significant association was noted between BoLA-DRB3.2*23 and *27 alleles ( p
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Association between BoLA-DRB3 and somatic cell count in Holstein Cattle from Argentina
Molecular biology reports, 2012Co-Authors: Laura Rosana Baltian, Shin-nosuke Takeshima, Yoko Aida, María Verónica Ripoli, S. Sanfilippo, Guillermo GiovambattistaAbstract:Different studies have proved that the resistance/susceptibility to mastitis is genetically determined. The major histocompatibility complex in cows is known as bovine lymphocyte antigen (BoLA). Genes from the BoLA have been associated with the occurrence of infectious diseases such as mastitis and leukosis, especially the BoLA-DRB gene. The object of the present study was to detect associations between BoLA-DRB3 alleles and somatic cell count (SCC), as an indicator of resistance/susceptibility to mastitis in Holstein Cattle (N = 123) from La Pampa, Argentina. Fisher’s exact test and Woolf–Haldane odds ratio were applied to study the association between SCC and BoLA-DRB3 allele frequencies. Significant association was noted between BoLA-DRB3.2*23 and *27 alleles (p < 0.05) and protective or susceptibility effects, respectively. In addition, alleles BoLA-DRB3.2*20 and *25 exhibit suggestive association with high SCC (p < 0.1). These results were partially in agreement with data reported from Japanese Holstein Cattle, but differed from those published by other authors. A possible explanation for the contrasting results could be that the mastitis is a multifactor disease caused by different pathogens. Moreover, most of the studies were carried out using PCR-RFLP method, which has less resolution than PCR-SBT because PCR-RFLP defined alleles included more than one sequenced alleles.
Laura Rosana Baltian - One of the best experts on this subject based on the ideXlab platform.
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Association between BoLA-DRB3 and somatic cell count in Holstein Cattle from Argentina
Molecular Biology Reports, 2012Co-Authors: Laura Rosana Baltian, María Verónica Ripoli, S. Sanfilippo, S. N. Takeshima, Y. Aida, Guillermo GiovambattistaAbstract:Different studies have proved that the resistance/susceptibility to mastitis is genetically determined. The major histocompatibility complex in cows is known as bovine lymphocyte antigen (BoLA). Genes from the BoLA have been associated with the occurrence of infectious diseases such as mastitis and leukosis, especially the BoLA-DRB gene. The object of the present study was to detect associations between BoLA-DRB3 alleles and somatic cell count (SCC), as an indicator of resistance/susceptibility to mastitis in Holstein Cattle ( N = 123) from La Pampa, Argentina. Fisher’s exact test and Woolf–Haldane odds ratio were applied to study the association between SCC and BoLA-DRB3 allele frequencies. Significant association was noted between BoLA-DRB3.2*23 and *27 alleles ( p
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Association between BoLA-DRB3 and somatic cell count in Holstein Cattle from Argentina
Molecular biology reports, 2012Co-Authors: Laura Rosana Baltian, Shin-nosuke Takeshima, Yoko Aida, María Verónica Ripoli, S. Sanfilippo, Guillermo GiovambattistaAbstract:Different studies have proved that the resistance/susceptibility to mastitis is genetically determined. The major histocompatibility complex in cows is known as bovine lymphocyte antigen (BoLA). Genes from the BoLA have been associated with the occurrence of infectious diseases such as mastitis and leukosis, especially the BoLA-DRB gene. The object of the present study was to detect associations between BoLA-DRB3 alleles and somatic cell count (SCC), as an indicator of resistance/susceptibility to mastitis in Holstein Cattle (N = 123) from La Pampa, Argentina. Fisher’s exact test and Woolf–Haldane odds ratio were applied to study the association between SCC and BoLA-DRB3 allele frequencies. Significant association was noted between BoLA-DRB3.2*23 and *27 alleles (p < 0.05) and protective or susceptibility effects, respectively. In addition, alleles BoLA-DRB3.2*20 and *25 exhibit suggestive association with high SCC (p < 0.1). These results were partially in agreement with data reported from Japanese Holstein Cattle, but differed from those published by other authors. A possible explanation for the contrasting results could be that the mastitis is a multifactor disease caused by different pathogens. Moreover, most of the studies were carried out using PCR-RFLP method, which has less resolution than PCR-SBT because PCR-RFLP defined alleles included more than one sequenced alleles.
Ji-feng Zhong - One of the best experts on this subject based on the ideXlab platform.
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solexa sequencing of novel and differentially expressed micrornas in testicular and ovarian tissues in Holstein Cattle
International Journal of Biological Sciences, 2011Co-Authors: Jin-ming Huang, Chang-fa Wang, Minghai Hou, Qinlei Hou, Lingling Wang, Tao Sun, Suqin Hang, Yundong Gao, Ji-feng ZhongAbstract:The posttranscriptional gene regulation mediated by microRNA plays an important role in the development and function of male and female reproductive organs and germ cells in mammals, including Cattle. In the present study, we identified novel and differentially expressed miRNAs in the testis and ovary in Holstein Cattle by combining the Solexa sequencing with bioinformatics. In total 100 and 104 novel pre-miRNAs were identified in testicular and ovarian tissues, encoding 122 and 136 mature miRNAs, respectively. Of these, 6 miRNAs appear to be bovine-specific. A total of 246 known miRNAs were co-expressed in the testicular and ovarian tissues. Of the known miRNAs, twenty-one testis-specific and nine ovary-specific (1-23 reads) were found. Approximately 30.5% of the known bovine miRNAs in this study were found to have >2-fold differential expression within the two respective reproductive organ systems. The putative miRNA target genes of miRNAs were involved in pathways associated with reproductive physiology. Both known and novel tissue-specific miRNAs are expressed by Real-time quantitative PCR analysis in dairy Cattle. This study expands the number of miRNAs known to be expressed in Cattle. The patterns of miRNAs expression differed significantly between the bovine testicular and ovarian tissues, which provide important information on sex differences in miRNA expression. Diverse miRNAs may play an important regulatory role in the development of the reproductive organs in Holstein Cattle.
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Two Novel SNPs in HSF1 Gene Are Associated with Thermal Tolerance Traits in Chinese Holstein Cattle
DNA and cell biology, 2010Co-Authors: Jin-ming Huang, Chang-fa Wang, Minghai Hou, Ji-feng ZhongAbstract:Heat-shock transcription factors (HSFs) play an important role in regulating heat stress response. The activation of heat-shock protein (HSP) genes is mediated by HSFs, which bind to promoters of HSP genes. In this research, two novel single nucleotide polymorphisms, T909C and G4693T, and their association with thermal tolerance were investigated in 951 Chinese Holstein Cattle. Linkage disequilibrium and haplotype construction were analyzed using SHEsis software. Four haplotypes were constructed, and nine haplotype combinations were found. Potassium content in erythrocytes (PCE), decreased rate of milk production (R), rectal temperature (RT), and heat-tolerance coefficient (HTC) were selected for the thermotolerance index. Association analysis showed that thermal tolerance in Chinese Holstein Cattle was significantly affected by T909C and G4693T. The PCE of cows with CC or TC genotype was lower than that of TT at the 909 position (p
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Genetic polymorphism of Nramp1 gene and correlation with mastitis in Holstein Cattle.
Yi chuan = Hereditas, 2009Co-Authors: Hong-mei Wang, Chang-fa Wang, Song-jia Lai, Ji-feng ZhongAbstract:In this research, PCR-SSCP technique was used to analyze the polymorphisms of the exon 11 of Nramp1 gene in Chinese Holstein Cattle (n=344), and correlation between polymorphisms of Nramp1 with somatic cell score (SCS) and milk production traits was analyzed. The results show that three genotypes namely AA, BB, and AB were detected. Allele A was predominant and the frequencies of alleles A and B were estimated to be 0.767 and 0.233, respectively. Chi-square test indicated that the polymorphic locus in Chinese Holstein fitted Hardy-Weinberg equilibrium (P>0.05). Sequencing analysis showed two polymorphic sites at positions 200 bp (C/G) and 254 bp (T/G), which resulted in amino acid alteration Ala356Pro and Leu374Met. The least squares means of SCS in Holstein Cattle was lower for genotype AA than that for genotypes AB and BB (P
S. Sanfilippo - One of the best experts on this subject based on the ideXlab platform.
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Association between BoLA-DRB3 and somatic cell count in Holstein Cattle from Argentina
Molecular Biology Reports, 2012Co-Authors: Laura Rosana Baltian, María Verónica Ripoli, S. Sanfilippo, S. N. Takeshima, Y. Aida, Guillermo GiovambattistaAbstract:Different studies have proved that the resistance/susceptibility to mastitis is genetically determined. The major histocompatibility complex in cows is known as bovine lymphocyte antigen (BoLA). Genes from the BoLA have been associated with the occurrence of infectious diseases such as mastitis and leukosis, especially the BoLA-DRB gene. The object of the present study was to detect associations between BoLA-DRB3 alleles and somatic cell count (SCC), as an indicator of resistance/susceptibility to mastitis in Holstein Cattle ( N = 123) from La Pampa, Argentina. Fisher’s exact test and Woolf–Haldane odds ratio were applied to study the association between SCC and BoLA-DRB3 allele frequencies. Significant association was noted between BoLA-DRB3.2*23 and *27 alleles ( p
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Association between BoLA-DRB3 and somatic cell count in Holstein Cattle from Argentina
Molecular biology reports, 2012Co-Authors: Laura Rosana Baltian, Shin-nosuke Takeshima, Yoko Aida, María Verónica Ripoli, S. Sanfilippo, Guillermo GiovambattistaAbstract:Different studies have proved that the resistance/susceptibility to mastitis is genetically determined. The major histocompatibility complex in cows is known as bovine lymphocyte antigen (BoLA). Genes from the BoLA have been associated with the occurrence of infectious diseases such as mastitis and leukosis, especially the BoLA-DRB gene. The object of the present study was to detect associations between BoLA-DRB3 alleles and somatic cell count (SCC), as an indicator of resistance/susceptibility to mastitis in Holstein Cattle (N = 123) from La Pampa, Argentina. Fisher’s exact test and Woolf–Haldane odds ratio were applied to study the association between SCC and BoLA-DRB3 allele frequencies. Significant association was noted between BoLA-DRB3.2*23 and *27 alleles (p < 0.05) and protective or susceptibility effects, respectively. In addition, alleles BoLA-DRB3.2*20 and *25 exhibit suggestive association with high SCC (p < 0.1). These results were partially in agreement with data reported from Japanese Holstein Cattle, but differed from those published by other authors. A possible explanation for the contrasting results could be that the mastitis is a multifactor disease caused by different pathogens. Moreover, most of the studies were carried out using PCR-RFLP method, which has less resolution than PCR-SBT because PCR-RFLP defined alleles included more than one sequenced alleles.
María Verónica Ripoli - One of the best experts on this subject based on the ideXlab platform.
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Association between BoLA-DRB3 and somatic cell count in Holstein Cattle from Argentina
Molecular Biology Reports, 2012Co-Authors: Laura Rosana Baltian, María Verónica Ripoli, S. Sanfilippo, S. N. Takeshima, Y. Aida, Guillermo GiovambattistaAbstract:Different studies have proved that the resistance/susceptibility to mastitis is genetically determined. The major histocompatibility complex in cows is known as bovine lymphocyte antigen (BoLA). Genes from the BoLA have been associated with the occurrence of infectious diseases such as mastitis and leukosis, especially the BoLA-DRB gene. The object of the present study was to detect associations between BoLA-DRB3 alleles and somatic cell count (SCC), as an indicator of resistance/susceptibility to mastitis in Holstein Cattle ( N = 123) from La Pampa, Argentina. Fisher’s exact test and Woolf–Haldane odds ratio were applied to study the association between SCC and BoLA-DRB3 allele frequencies. Significant association was noted between BoLA-DRB3.2*23 and *27 alleles ( p
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Association between BoLA-DRB3 and somatic cell count in Holstein Cattle from Argentina
Molecular biology reports, 2012Co-Authors: Laura Rosana Baltian, Shin-nosuke Takeshima, Yoko Aida, María Verónica Ripoli, S. Sanfilippo, Guillermo GiovambattistaAbstract:Different studies have proved that the resistance/susceptibility to mastitis is genetically determined. The major histocompatibility complex in cows is known as bovine lymphocyte antigen (BoLA). Genes from the BoLA have been associated with the occurrence of infectious diseases such as mastitis and leukosis, especially the BoLA-DRB gene. The object of the present study was to detect associations between BoLA-DRB3 alleles and somatic cell count (SCC), as an indicator of resistance/susceptibility to mastitis in Holstein Cattle (N = 123) from La Pampa, Argentina. Fisher’s exact test and Woolf–Haldane odds ratio were applied to study the association between SCC and BoLA-DRB3 allele frequencies. Significant association was noted between BoLA-DRB3.2*23 and *27 alleles (p < 0.05) and protective or susceptibility effects, respectively. In addition, alleles BoLA-DRB3.2*20 and *25 exhibit suggestive association with high SCC (p < 0.1). These results were partially in agreement with data reported from Japanese Holstein Cattle, but differed from those published by other authors. A possible explanation for the contrasting results could be that the mastitis is a multifactor disease caused by different pathogens. Moreover, most of the studies were carried out using PCR-RFLP method, which has less resolution than PCR-SBT because PCR-RFLP defined alleles included more than one sequenced alleles.
