The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Hugh S. Taylor - One of the best experts on this subject based on the ideXlab platform.
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Endometriosis Located Proximal to or Remote From the Uterus Differentially Affects Uterine Gene Expression
Reproductive Sciences, 2016Co-Authors: Hanyia Naqvi, Ramanaiah Mamillapalli, Graciela Krikun, Hugh S. TaylorAbstract:The mechanisms that lead to the altered uterine gene expression in women with endometriosis are poorly understood. Are these changes in gene expression mediated by proximity to endometriotic lesions or is endometriosis a systemic disease where the effect is independent of proximity to the uterus? To answer this question, we created endometriosis in a murine model either in the peritoneal cavity (proximal) or at a subcutaneous remote site (distal). The expression of several genes that are involved in endometrial receptivity ( Homeobox A10 [HoxA10] , Homeobox A11 [Hoxa11] , insulin-like growth factor binding protein 1 [ Igfbp1 ], Kruppel-like factor 9 [ Klf9 ], and progesterone receptor [ Pgr ]) was measured in the eutopic endometrium of mice transplanted with either proximal or distal endometriosis lesions. Decreased expression of HoxA10 , Igfbp1 , Klf9 , and total Pgr genes was observed in the eutopic endometrium of mice with peritoneal endometriosis. In the mice with distal lesions, overall expression of these genes was not as severely affected, however, Igfbp1 expression was similarly decreased and the effect on Pgr was more pronounced. Endometriosis does have a systemic effect that varies with distance to the end organ. However, even remote disease selectively and profoundly alters the expression of genes such as Pgr . This is the first controlled experiment demonstrating that endometriosis is not simply a local peritoneal disease. Selective alteration of genes critical for endometrial receptivity and endometriosis propagation may be systemic. Similarly, systemic effects of endometriosis on other organs may also be responsible for the widespread manifestations of the disease.
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the relationship among hoxA10 estrogen receptor α progesterone receptor and progesterone receptor b proteins in rectosigmoid endometriosis a tissue microarray study
Reproductive Sciences, 2015Co-Authors: A Zanatta, Hugh S. Taylor, R M A Pereira, A M Rocha, Bruno Cogliati, Edmund Chada Baracat, Eduardo L A Motta, P SerafiniAbstract:Background:Very few studies have evaluated the expression of Homeobox A10 (HOXA10) and steroid (estrogen and progesterone) receptors exclusively in deep endometriosis. Conclusions drawn from studies evaluating peritoneal and ovarian endometriosis are usually generalized to explain the pathogenesis of the disease as a whole. We aimed to evaluate the expression of HOXA10, estrogen receptor α (ER-α), progesterone receptor (PR), and PR-B in rectosigmoid endometriosis (RE), a typical model of deep disease.Methods:We used RE samples from 18 consecutive patients to construct tissue microarray blocks. Nine patients each were operated during the proliferative and secretory phases of the menstrual cycle. We quantified the expressions of proteins by immunohistochemistry using the modified Allred score.Result:The HOXA10 was expressed in the stroma of nodules during the secretory phase in 5 of the 18 patients. Expression of ER-α (in 16 of 18 patients), PR (in 17 of 18 patients), and PR-B (17 of 18 patients) was modera...
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The Relationship Among HOXA10, Estrogen Receptor α, Progesterone Receptor, and Progesterone Receptor B Proteins in Rectosigmoid Endometriosis: A Tissue Microarray Study
Reproductive Sciences, 2015Co-Authors: Alysson Zanatta, Hugh S. Taylor, R M A Pereira, A M Rocha, Bruno Cogliati, Edmund Chada Baracat, Eduardo L A Motta, Paulo Cesar SerafiniAbstract:Background Very few studies have evaluated the expression of Homeobox A10 (HOXA10) and steroid (estrogen and progesterone) receptors exclusively in deep endometriosis. Conclusions drawn from studies evaluating peritoneal and ovarian endometriosis are usually generalized to explain the pathogenesis of the disease as a whole. We aimed to evaluate the expression of HOXA10, estrogen receptor α (ER-α), progesterone receptor (PR), and PR-B in rectosigmoid endometriosis (RE), a typical model of deep disease. Methods We used RE samples from 18 consecutive patients to construct tissue microarray blocks. Nine patients each were operated during the proliferative and secretory phases of the menstrual cycle. We quantified the expressions of proteins by immunohistochemistry using the modified Allred score. Result The HOXA10 was expressed in the stroma of nodules during the secretory phase in 5 of the 18 patients. Expression of ER-α (in 16 of 18 patients), PR (in 17 of 18 patients), and PR-B (17 of 18 patients) was moderate to strong in the glands and stroma of nodules during both phases. Expression of both PR ( P = .023) and PR-B ( P = .024) was significantly greater during the secretory phase. Conclusion The HOXA10 is expressed in RE, where it likely imparts the de novo identity of endometriotic lesions. The ER-α, PR, and PR-B are strongly expressed in RE, which differs from previous studies investigating peritoneal and ovarian lesions. This suggests different routes of pathogenesis for each of the 3 types of endometriosis.
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A novel role for the AAA ATPase spastin as a HOXA10 transcriptional corepressor in Ishikawa endometrial cells.
Molecular endocrinology (Baltimore Md.), 2011Co-Authors: Gaurang S. Daftary, Amy M. Tetrault, Elisa M. Jorgensen, Jennifer L. Sarno, Hugh S. TaylorAbstract:Homeobox A10 (HOXA10), a transcription factor required for uterine development and embryo receptivity, functions downstream of estrogen and progesterone in uterine endometrium. HOXA10 represses endometrial expression of empty spiracles Homeobox 2 (EMX2), the human ortholog of Drosophila empty spiracles. The ATPases associated with various cellular activities (AAA) ATPase spastin has a well-characterized role in neurotransmitter trafficking. In this study, we characterize a novel role of spastin in transcriptional regulation. We identified spastin as a novel component of the HOXA10 transcriptional complex in Ishikawa nuclear extracts by immunoprecipitation and mass spectrophotometry. Using EMX2 as a model endometrial HOXA10 target gene, we show that the HOXA10-spastin corepressor complex bound the EMX2 promoter in chromatin immunoprecipitation assays. HOXA10 has been previously shown to repress endometrial EMX2 expression. We further observed that, although cotransfection of HOXA10 and spastin continued to repress endometrial EMX2-luciferase expression, the repression was reversed when spastin small interfering RNA was cotransfected with HOXA10. Mutations in the nuclear localization signal sequences of spastin abrogated not only its nuclear translocation but also its colocalization with HOXA10 as well as reversed EMX2-luciferase repression. Here, we describe a novel role for the AAA ATPase spastin in Ishikawa cells as a HOXA10 corepressor of EMX2. Uterine EMX2 levels are inversely related to embryo implantation rates. HOXA10 acts downstream of progesterone and has been shown to facilitate embryo implantation through regulation of endometrial EMX2 expression. Endometrial spastin, therefore, likely has a novel function downstream of estrogen and progesterone in implantation biology as a cofactor of HOXA10.
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Cigarette Smoke Increases Progesterone Receptor and Homeobox A10 Expression in Human Endometrium and Endometrial Cells: A Potential Role in the Decreased Prevalence of Endometrial Pathology in Smokers
Biology of reproduction, 2011Co-Authors: Yuping Zhou, Elisa M. Jorgensen, Ye Gan, Hugh S. TaylorAbstract:Cigarette smoking has long been tied to a multitude of poor health outcomes; however, in reproductive biology, smoking has shown several unintuitive findings. Smoking is associated with significantly decreased rates of endometriosis and endometrial cancer. Here, we show that treatment with cigarette smoke extract leads to increased mRNA and protein expression of Homeobox A10 (HOXA10) and progesterone receptor (PGR) as well as more rapid decidualization of endometrial stromal cells in vitro. In vivo, mice exposed to cigarette smoke similarly showed increased expression of HOXA10 and PGR in the endometrium. Both HOXA10 and PGR drive endometrial differentiation and are suppressed in endometrial tumors and in endometriosis. The increased expression found upon exposure to cigarette smoke may provide a protective effect, mediating the decreased incidence of endometrial disease among smokers. This mechanism contrasts with the accepted paradigm that the effects of smoking on the uterus are secondary to ovarian alterations rather than direct effects on endometrium as demonstrated here.
Masashi Shiiba - One of the best experts on this subject based on the ideXlab platform.
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State of Homeobox A10 expression as a putative prognostic marker for oral squamous cell carcinoma
Oncology reports, 2009Co-Authors: Masanobu Yamatoji, Atsushi Kasamatsu, Yukio Yamano, Kentaro Sakuma, Kenji Ogoshi, Manabu Iyoda, Keiji Shinozuka, Katsunori Ogawara, Yuichi Takiguchi, Masashi ShiibaAbstract:Homeobox (HOX) A10, the regulator of embryonic morphogenesis and differentiation, is aberrantly expressed in several cancer types. Our previous study using microarray technology showed that significant up-regulation of HOXA10 occurs in oral squamous cell carcinoma (OSCC)-derived cell lines compared to human normal oral keratinocytes (HNOKs). The aim of the current study was to examine the status of HOXA10 mRNA and protein expression in OSCC-derived cell lines and human primary OSCCs. HOXA10 mRNA was up-regulated in six OSCC-derived cell lines compared with HNOKs and in primary OSCCs by using real-time quantitative reverse transcriptase-polymerase chain reaction. Immunohistochemistry data indicated that HOXA10 protein expression levels were consistent with mRNA expression status in OSCC-derived cell lines and primary OSCCs. Furthermore, HOXA10 expression status was correlated with the TNM stage (P
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state of Homeobox A10 expression as a putative prognostic marker for oral squamous cell carcinoma
Oncology Reports, 2009Co-Authors: Masanobu Yamatoji, Atsushi Kasamatsu, Yukio Yamano, Kentaro Sakuma, Kenji Ogoshi, Manabu Iyoda, Keiji Shinozuka, Katsunori Ogawara, Yuichi Takiguchi, Masashi ShiibaAbstract:Homeobox (HOX) A10, the regulator of embryonic morphogenesis and differentiation, is aberrantly expressed in several cancer types. Our previous study using microarray technology showed that significant up-regulation of HOXA10 occurs in oral squamous cell carcinoma (OSCC)-derived cell lines compared to human normal oral keratinocytes (HNOKs). The aim of the current study was to examine the status of HOXA10 mRNA and protein expression in OSCC-derived cell lines and human primary OSCCs. HOXA10 mRNA was up-regulated in six OSCC-derived cell lines compared with HNOKs and in primary OSCCs by using real-time quantitative reverse transcriptase-polymerase chain reaction. Immunohistochemistry data indicated that HOXA10 protein expression levels were consistent with mRNA expression status in OSCC-derived cell lines and primary OSCCs. Furthermore, HOXA10 expression status was correlated with the TNM stage (P<0.05). These results indicate that HOXA10 expression could contribute to cancer progression and prognosis and that HOXA10 may be a potential diagnostic marker and a therapeutic target for OSCCs.
Shuhong Zhao - One of the best experts on this subject based on the ideXlab platform.
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Production of Homeobox A10 gene transgenic pigs by somatic cell nuclear transfer
Journal of Integrative Agriculture, 2019Co-Authors: Qian Xiao, Ruiyi Lin, Changzhi Zhao, Hai-yan Wang, Sheng-song Xie, Shuhong ZhaoAbstract:Abstract Homeobox A10 (HoxA10) gene is one of the most important candidate genes associated with the reproductive performance of humans and mice. Overexpression of HoxA10 in mouse endometrium can increase litter size. Moreover, HoxA10 plays a key role in regulating the embryo implantation of sows. This study aimed to generate transgenic pigs using HoxA10 via somatic cell nuclear transfer (SCNT). We established seven HoxA10-transgenic cell lines, and two of the cell lines were selected as nuclear donors for the transfer. A total of 1270 cloned embryos were generated and transferred to five surrogate mothers (Landrace×Yorkshire). Eight cloned male piglets were produced including one with cryptorchidism. Six transgenic piglets grew up healthy and produced 56 offspring. Finally, we obtained six transgenic male pigs and 26 transgenic positive offspring that can be used to further study the regulatory mechanism of HoxA10 on the reproductive performance of pigs.
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Inducible overexpression of porcine Homeobox A10 in the endometrium of transgenic mice
Journal of Integrative Agriculture, 2016Co-Authors: Ruiyi Lin, Changzhi Zhao, Qian Xiao, Chen Shangshang, Shuhong ZhaoAbstract:Abstract Homeobox A10 (HOXA10) is a well-known transcription factor that plays an important role in directing endometrial differentiation and establishing the conditions required for implantation. Interestingly, the expression level of HOXA10 may be associated with litter size. To study the effects of the porcine HOXA10 promoter fragment on the expression of HOXA10 gene in vivo, we generated a transgenic mouse model using pronuclear microinjection, and measured the expression of HOXA10 in the endometrium. There was no difference in the expression level of HOXA10 between transgenic and wild-type mice in the absence of hormone stimulation. However, following treatment with progesterone and estradiol benzoate, the expression level of HOXA10 was significantly increased in transgenic mice compared with that of wild-type mice. Furthermore, the litter size of transgenic females was larger than that of wild-type females (7.02±1.73 vs. 6.48±1.85; P=0.14). Moreover, the difference of litter size was greater in the later parities (7.33±1.62 vs. 6.37±2.02; P=0.08) compared with the first parity (6.76±1.81 vs. 6.61±1.67; P=0.77) between transgenic and wild-type mice. Therefore, our transgenic mouse model provides exciting insights regarding the actions of HOXA10 and its hormone-inducible promoter in vivo. The present study offers valuable proof of principle to develop transgenic pigs with a hormone-inducible promoter regulating HOXA10 to alter litter size.
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identification of lncrnas mrnas related to endometrium function regulated by Homeobox A10 in ishikawa cells
Cell Biology International, 2015Co-Authors: Ruiyi Lin, Changzhi Zhao, Lu Jing, Shuhong ZhaoAbstract:As a well-known transcription factor, Homeobox A10 (HOXA10) regulates a large number of downstream target genes, leading to the proper function development of endometrium for embryo implantation. The change of HOXA10 gene expression level can alter the expressions of many other genes, including coding and noncoding transcripts. In our study, mRNA and LncRNA expression profiles screening was performed by microarray when the HOXA10 gene expression level increased in Ishikawa cells. A total of 907 mRNAs and 1,026 LncRNAs were identified as differentially expressed transcripts (Fold Change ≥2, P-value <0.05, and Q-value <0.05) between HOXA10 overexpressed and control Ishikawa cells. Further analysis identified that these mRNAs participated in various biological processes, such as blood vessel development, cell adhesion, cell cycle, etc. Also, 14 enhancer-like LncRNAs and 108 LincRNAs with their nearby mRNAs were identified as coregulated transcripts. Our results showed that the mRNA and LncRNA expression profiles differed significantly between the two groups and provided useful information for further studying the molecular mechanisms of HOXA10 in endometrium.
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Identification of LncRNAs/mRNAs related to endometrium function regulated by Homeobox A10 in Ishikawa cells.
Cell biology international, 2015Co-Authors: Ruiyi Lin, Changzhi Zhao, Lu Jing, Shuhong ZhaoAbstract:As a well-known transcription factor, Homeobox A10 (HOXA10) regulates a large number of downstream target genes, leading to the proper function development of endometrium for embryo implantation. The change of HOXA10 gene expression level can alter the expressions of many other genes, including coding and noncoding transcripts. In our study, mRNA and LncRNA expression profiles screening was performed by microarray when the HOXA10 gene expression level increased in Ishikawa cells. A total of 907 mRNAs and 1,026 LncRNAs were identified as differentially expressed transcripts (Fold Change ≥2, P-value
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Molecular characterization and identification of the E2/P4 response element in the porcine HOXA10 gene
Molecular and cellular biochemistry, 2012Co-Authors: Dechao Song, Shuhong ZhaoAbstract:Homeobox A10 (HOXA10), a well-known transcriptional factor that can be regulated by estrogen (E(2)) and progesterone (P(4)), is necessary not only for endometrial differentiation but also for establishing the conditions required for implantation. However, little research has focused on the regulation of the porcine HOXA10 gene. In this study, we aimed to (1) characterize the genetic structure of the porcine HOXA10 gene, (2) analyze the tissue expression pattern and differential expression levels in the endometrium of HOXA10 in different developmental stages of Meishan and commercial Yorkshire pigs, and (3) identify the E2/P4 response element in the promoter region of the porcine HOXA10 gene and verify that it induces HOXA10 in human endometrial adenocarcinoma (Ishikawa) cells. The results indicated that the cDNA of porcine HOXA10 was 2,628 bp in length with an open reading frame (ORF) of 1,236 bp encoding a peptide of 411-amino acid residues, which showed 90 and 95 % sequence similarity to that of human and mouse homologs, respectively. Semiquantitative RT-PCR confirmed that the porcine HOXA10 gene is highly expressed in the endometrium, bladder and kidney. Real-time PCR showed that the expression of HOXA10 was significantly higher on day 15 of gestation (gd 15) in comparison to gd 26 (P < 0.01), gd 50 (P < 0.01) and day 15 of the estrous cycle (ed 15) in the endometrium of Meishan pigs. In contrast, the highest expression in the endometrium of Yorkshire pigs was on gd 50. Moreover, the abundance of HOXA10 mRNA was the highest on gd 15 in Meishan pigs than in any other stage tested in the two breeds. Deletion analysis and reporter expression assays identified a promoter region (-1044 bp to +18 bp) which is responsible for E(2)- and P(4)-induced HOXA10 transcription. Moreover, this promoter region enhanced the E2/P4-induced HOXA10 expression in Ishikawa cells. In conclusion, we identified an E(2)/P(4) response element of the porcine HOXA10 gene for the first time. The data from the present study contribute to the mechanism by which the porcine HOXA10 gene regulates embryo implantation and prolificacy traits.
Masanobu Yamatoji - One of the best experts on this subject based on the ideXlab platform.
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State of Homeobox A10 expression as a putative prognostic marker for oral squamous cell carcinoma
Oncology reports, 2009Co-Authors: Masanobu Yamatoji, Atsushi Kasamatsu, Yukio Yamano, Kentaro Sakuma, Kenji Ogoshi, Manabu Iyoda, Keiji Shinozuka, Katsunori Ogawara, Yuichi Takiguchi, Masashi ShiibaAbstract:Homeobox (HOX) A10, the regulator of embryonic morphogenesis and differentiation, is aberrantly expressed in several cancer types. Our previous study using microarray technology showed that significant up-regulation of HOXA10 occurs in oral squamous cell carcinoma (OSCC)-derived cell lines compared to human normal oral keratinocytes (HNOKs). The aim of the current study was to examine the status of HOXA10 mRNA and protein expression in OSCC-derived cell lines and human primary OSCCs. HOXA10 mRNA was up-regulated in six OSCC-derived cell lines compared with HNOKs and in primary OSCCs by using real-time quantitative reverse transcriptase-polymerase chain reaction. Immunohistochemistry data indicated that HOXA10 protein expression levels were consistent with mRNA expression status in OSCC-derived cell lines and primary OSCCs. Furthermore, HOXA10 expression status was correlated with the TNM stage (P
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state of Homeobox A10 expression as a putative prognostic marker for oral squamous cell carcinoma
Oncology Reports, 2009Co-Authors: Masanobu Yamatoji, Atsushi Kasamatsu, Yukio Yamano, Kentaro Sakuma, Kenji Ogoshi, Manabu Iyoda, Keiji Shinozuka, Katsunori Ogawara, Yuichi Takiguchi, Masashi ShiibaAbstract:Homeobox (HOX) A10, the regulator of embryonic morphogenesis and differentiation, is aberrantly expressed in several cancer types. Our previous study using microarray technology showed that significant up-regulation of HOXA10 occurs in oral squamous cell carcinoma (OSCC)-derived cell lines compared to human normal oral keratinocytes (HNOKs). The aim of the current study was to examine the status of HOXA10 mRNA and protein expression in OSCC-derived cell lines and human primary OSCCs. HOXA10 mRNA was up-regulated in six OSCC-derived cell lines compared with HNOKs and in primary OSCCs by using real-time quantitative reverse transcriptase-polymerase chain reaction. Immunohistochemistry data indicated that HOXA10 protein expression levels were consistent with mRNA expression status in OSCC-derived cell lines and primary OSCCs. Furthermore, HOXA10 expression status was correlated with the TNM stage (P<0.05). These results indicate that HOXA10 expression could contribute to cancer progression and prognosis and that HOXA10 may be a potential diagnostic marker and a therapeutic target for OSCCs.
Yuichi Takiguchi - One of the best experts on this subject based on the ideXlab platform.
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State of Homeobox A10 expression as a putative prognostic marker for oral squamous cell carcinoma
Oncology reports, 2009Co-Authors: Masanobu Yamatoji, Atsushi Kasamatsu, Yukio Yamano, Kentaro Sakuma, Kenji Ogoshi, Manabu Iyoda, Keiji Shinozuka, Katsunori Ogawara, Yuichi Takiguchi, Masashi ShiibaAbstract:Homeobox (HOX) A10, the regulator of embryonic morphogenesis and differentiation, is aberrantly expressed in several cancer types. Our previous study using microarray technology showed that significant up-regulation of HOXA10 occurs in oral squamous cell carcinoma (OSCC)-derived cell lines compared to human normal oral keratinocytes (HNOKs). The aim of the current study was to examine the status of HOXA10 mRNA and protein expression in OSCC-derived cell lines and human primary OSCCs. HOXA10 mRNA was up-regulated in six OSCC-derived cell lines compared with HNOKs and in primary OSCCs by using real-time quantitative reverse transcriptase-polymerase chain reaction. Immunohistochemistry data indicated that HOXA10 protein expression levels were consistent with mRNA expression status in OSCC-derived cell lines and primary OSCCs. Furthermore, HOXA10 expression status was correlated with the TNM stage (P
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state of Homeobox A10 expression as a putative prognostic marker for oral squamous cell carcinoma
Oncology Reports, 2009Co-Authors: Masanobu Yamatoji, Atsushi Kasamatsu, Yukio Yamano, Kentaro Sakuma, Kenji Ogoshi, Manabu Iyoda, Keiji Shinozuka, Katsunori Ogawara, Yuichi Takiguchi, Masashi ShiibaAbstract:Homeobox (HOX) A10, the regulator of embryonic morphogenesis and differentiation, is aberrantly expressed in several cancer types. Our previous study using microarray technology showed that significant up-regulation of HOXA10 occurs in oral squamous cell carcinoma (OSCC)-derived cell lines compared to human normal oral keratinocytes (HNOKs). The aim of the current study was to examine the status of HOXA10 mRNA and protein expression in OSCC-derived cell lines and human primary OSCCs. HOXA10 mRNA was up-regulated in six OSCC-derived cell lines compared with HNOKs and in primary OSCCs by using real-time quantitative reverse transcriptase-polymerase chain reaction. Immunohistochemistry data indicated that HOXA10 protein expression levels were consistent with mRNA expression status in OSCC-derived cell lines and primary OSCCs. Furthermore, HOXA10 expression status was correlated with the TNM stage (P<0.05). These results indicate that HOXA10 expression could contribute to cancer progression and prognosis and that HOXA10 may be a potential diagnostic marker and a therapeutic target for OSCCs.