The Experts below are selected from a list of 187941 Experts worldwide ranked by ideXlab platform
Yoshihiro Kawaoka - One of the best experts on this subject based on the ideXlab platform.
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soluble recombinant hemagglutinin protein of h1n1pdm09 influenza virus elicits cross protection against a lethal h5n1 challenge in mice
Frontiers in Microbiology, 2019Co-Authors: Shinya Yamada, Atsuhiro Yasuhara, Yoshihiro KawaokaAbstract:Currently, influenza vaccines are produced using embryonated chicken eggs. Recently, recombinant influenza vaccines have been developed as a potential alternative to egg-grown vaccines. In this study, we evaluated the efficacy of soluble recombinant hemagglutinin (HA) protein produced in Human Cell Culture (Expi293F Cells) as an influenza vaccine against homosubtypic and heterosubtypic influenza virus challenges in mice. Mice were immunized intramuscularly with purified soluble HA protein of H1N1pdm09 virus and then challenged with a lethal dose of H1N1pdm09, seasonal H3N2, or highly pathogenic avian influenza (HPAI) H5N1 virus. Vaccinated mice showed better morbidity than mock-vaccinated mice following H1N1pdm09 challenge. By contrast, all mice died following H3N2 challenge. Interestingly, all vaccinated mice survived challenge with H5N1 virus, whereas all mock-vaccinated mice died. These results suggest that intramuscular immunization with recombinant HA proteins produced in Expi 293F Cells could be of value in influenza vaccine strategies.
John Hwa Lee - One of the best experts on this subject based on the ideXlab platform.
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Safety implication of Salmonella based BruCella vaccine candidate in mice and in vitro Human Cell Culture.
Vaccine, 2018Co-Authors: Jonathan Lalsiamthara, Amal Senevirathne, John Hwa LeeAbstract:Abstract An anti-BruCella vaccine candidate comprising rough Salmonella vector delivering BruCella antigens was developed. This system provides a platform for live BruCella-free vaccine development as it can mimic active-intraCellular infection of BruCella organism. Exploiting this phenomenon thus provides significant protection at a single dose and also re-assured the safety. To date, no Human anti-BruCella vaccines are available, owing to the lack of safe and effective formulation. This study investigated the safety of the vaccine formulation in mice model and in vitro Human Cell Cultures. The experiment was designed to determine the LD50 of the vaccine formulation. The vaccine formulation did not induce any mortality even when mice were administered at 8 × 109 CFU per oral or per subcutaneous (SC), which was 100-times more than the actual vaccine dose intended for mice model. In contrast, wild-type (WT) Salmonella positive control strain induced 100% mortality at 8 × 107 CFU per mice via oral or SC routes. Interaction of the vaccine with phagocytic (THP-1 derived macrophage) and non-phagocytic (Caco-2) Human Cell lines as well as Human PBMC was investigated. In in vitro experiments, inflammatory and pyretic cytokines TNF-α, and IL-1β inductions were significantly lower in vaccine group as compared to WT group. Further, apoptosis, nitric oxide synthase and cytotoxicity inductions were comparable and not exacerbated, given that the strain is based on a rough bacterial vector that may have endotoxic lipid-A more readily exposed. These findings corroborated that the vaccine formulation is highly safe in mice model and is relatively mild in the induction of inflammatory cytokines and Cellular changes in Human Cell lines.
Esteban C Dellangelica - One of the best experts on this subject based on the ideXlab platform.
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zinc transporter 2 slc30a2 can suppress the vesicular zinc defect of adaptor protein 3 depleted fibroblasts by promoting zinc accumulation in lysosomes
Experimental Cell Research, 2007Co-Authors: Juan M Falconperez, Esteban C DellangelicaAbstract:Zinc accumulation in the lumen of cytoplasmic vesicles is one of the mechanisms by which Cells can store significant amounts of this essential but potentially toxic biometal. Previous studies had demonstrated reduced vesicular zinc levels in fibroblasts from mutant mice deficient in adaptor protein 3 (AP-3), a complex involved in protein trafficking to late endosomes and lysosomes. We have observed a similar phenotype in the Human fibroblastoid Cell line, M1, upon small interference RNA-mediated AP-3 knockdown. A survey of the expression and localization of zinc transporter (ZnT) family members identified ZnT2, ZnT3, and ZnT4 as likely mediators of vesicular zinc accumulation in M1 Cells. Expression of green fluorescence protein (GFP)-tagged ZnT2 and ZnT3 promoted accumulation of vesicular zinc as visualized using the indicator zinquin. Moreover, GFP-ZnT2 overexpression elicited a significant accumulation of zinc within mature lysosomes, which in untransfected M1 Cells contained little or no chelatable zinc, and restored the zinc storage capability of AP-3-deficient Cells. These results suggest that ZnT2 can facilitate vesicular zinc accumulation independently of AP-3 function, and validate the M1 fibroblastoid line as a Human Cell Culture system amenable to the study of vesicular zinc regulation using techniques compatible with functional genomic approaches.
Js Rhim - One of the best experts on this subject based on the ideXlab platform.
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In vitro Human Cell Culture models for the study of prostate cancer.
Prostate cancer and prostatic diseases, 2000Co-Authors: Js RhimAbstract:Prostate cancer is the most commonly diagnosed malignancy in the American men and the second leading cause of male cancer death in the United States. Despite its high incidence, the molecular and genetic events involved in prostate cancer progression remain poorly understood. A hurdle in understanding the molecular genetic changes in prostate cancer has been the difficulty in establishing premalignant lesions and primary prostate tumors as in vitro Cell Cultures. Primary epithelial Cells grow for a finite life span and then senesce. Immortalization is defined by continuous growth of otherwise senescing Cells and is believed to represent an early stage in tumor progression. In order to examine these early stages, we and others have developed in vitro models of prostate epithelial Cell immortalization. Because prostate cancer is a multistep, progressive disease with a typical onset later in life and with an unusually high number of latent cases that do not develop into clinically manifest cancer, the steps in the progression to malignancy are of particular interest. To understand the many factors that are suspected to contribute to the development of this malignancy, there is a need for an in vitro multistep Human prostate epithelial (HPE) Culture system. These models have been extremely important in identifying genetic and molecular changes involved in prostate cancer progression. Prostate Cancer and Prostatic Diseases (2000) 3, 229-235
F Stagnitti - One of the best experts on this subject based on the ideXlab platform.
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Assessing health risk of reclaimed water using Human Cell Culture.
Water, 2010Co-Authors: Margaret Ackland, Agnieszka Michalczyk, David Freestone, F StagnittiAbstract:For risk analysis of reclaimed water, current animal and toxicity testing may not detect subtle effects such as interactions that could contribute to complex diseases such as cancers that develop over a long period of time. There is a need for assays that can be validated against known Human physiological processes. We have previously validated sensitive Human Cell Culture assays for their responsiveness to agents that induce carcinogenesis in vivo. In this initial study we analysed the effects of three batches of reclaimed water on Human colonic Cells. At concentrations of up to 10-fold, they had no significant effect on the Cellular markers, indicating an overall lack of biological activity. The assay has potential but needs to be refined to maximise its sensitivity.