The Experts below are selected from a list of 96 Experts worldwide ranked by ideXlab platform
Christian David - One of the best experts on this subject based on the ideXlab platform.
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toward clinical x ray phase contrast ct demonstration of enhanced soft tissue contrast in Human Specimen
Investigative Radiology, 2010Co-Authors: Tilman Donath, Franz Pfeiffer, O Bunk, C Grunzweig, Eckhard Hempel, Stefan Popescu, Peter Vock, Christian DavidAbstract:X-ray computed tomography (CT) using phase contrast can provide images with greatly enhanced soft-tissue contrast in comparison to conventional attenuation-based CT. We report on the first scan of a Human Specimen recorded with a phase-contrast CT system based on an x-ray grating interferometer and a conventional x-ray tube source. Feasibility and potential applications of preclinical and clinical phase-contrast CT are discussed.
Franz Pfeiffer - One of the best experts on this subject based on the ideXlab platform.
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grating based phase contrast ct pcct histopathological correlation of Human liver cirrhosis and hepatocellular carcinoma Specimen
Journal of Clinical Pathology, 2020Co-Authors: Melanie A Kimm, Franz Pfeiffer, Marian Willner, Enken Drecoll, Julia Herzen, Peter B Noel, Ernst J Rummeny, Alexander A FingerleAbstract:Aims To correlate signal intensities in grating-based phase-contrast CT (PCCT) images obtained at a synchrotron light source and a conventional X-ray source with tissue components in Human liver cirrhosis and hepatocellular carcinoma (HCC) Specimen. Methods Study approval was obtained by the institutional review board. Human Specimen of liver cirrhosis and HCC were imaged at experimental grating-based PCCT setups using either a synchrotron radiation source or a conventional X-ray tube. Tissue samples were sectioned and processed for H&E and Elastica van Gieson staining. PCCT and histological images were manually correlated. Depending on morphology and staining characteristics tissue components like fibrosis, HCC, inflammation, connective tissue and necrosis were differentiated and visually correlated with signal intensity in PCCT images using a 5-point Likert scale with normal liver parenchyma as a reference. Results Grating-based PCCT images of Human cirrhotic liver and HCC Specimen showed high soft-tissue contrast allowing correlation with histopathological sections. Signal intensities were similar in both setups independent of the nature of the radiation source. Connective tissue and areas of haemorrhage displayed the highest signal intensities, fibrotic liver tissue the lowest. Conclusions Grating-based PCCT provides comparable results for the characterisation of Human Specimen of liver cirrhosis and HCC using either a synchrotron light source or a conventional X-ray tube. Due to its high soft-tissue contrast and its applicability to conventional X-ray tubes grating-based PCCT holds potential for preclinical research and virtual histology applications.
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toward clinical x ray phase contrast ct demonstration of enhanced soft tissue contrast in Human Specimen
Investigative Radiology, 2010Co-Authors: Tilman Donath, Franz Pfeiffer, O Bunk, C Grunzweig, Eckhard Hempel, Stefan Popescu, Peter Vock, Christian DavidAbstract:X-ray computed tomography (CT) using phase contrast can provide images with greatly enhanced soft-tissue contrast in comparison to conventional attenuation-based CT. We report on the first scan of a Human Specimen recorded with a phase-contrast CT system based on an x-ray grating interferometer and a conventional x-ray tube source. Feasibility and potential applications of preclinical and clinical phase-contrast CT are discussed.
Tilman Donath - One of the best experts on this subject based on the ideXlab platform.
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toward clinical x ray phase contrast ct demonstration of enhanced soft tissue contrast in Human Specimen
Investigative Radiology, 2010Co-Authors: Tilman Donath, Franz Pfeiffer, O Bunk, C Grunzweig, Eckhard Hempel, Stefan Popescu, Peter Vock, Christian DavidAbstract:X-ray computed tomography (CT) using phase contrast can provide images with greatly enhanced soft-tissue contrast in comparison to conventional attenuation-based CT. We report on the first scan of a Human Specimen recorded with a phase-contrast CT system based on an x-ray grating interferometer and a conventional x-ray tube source. Feasibility and potential applications of preclinical and clinical phase-contrast CT are discussed.
Carolina Sousa - One of the best experts on this subject based on the ideXlab platform.
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Gluten Immunogenic Peptides as Standard for the Evaluation of Potential Harmful Prolamin Content in Food and Human Specimen
Nutrients, 2018Co-Authors: Ángel Cebolla, Laura Coto, María De Lourdes Moreno, Carolina SousaAbstract:Gluten is a complex mixture of storage proteins in cereals like wheat, barley, and rye. Prolamins are the main components of gluten. Their high content in proline and glutamine makes them water-insoluble and difficult to digest in the gastrointestinal tract. Partial digestion generates peptide sequences which trigger immune responses in celiac and gluten-sensitive patients. Gluten detection in food is challenging because of the diversity, in various food matrices, of protein proportions or modifications and the huge number of immunogenic sequences with differential potential immunoactivity. Attempts to develop standard reference materials have been unsuccessful. Recent studies have reported the detection of a limited number of dominant Gluten Immunogenic Peptides (GIP) that share similarities to epitopes presented in the α-gliadin 33-mer, which showed to be highly proteolytic resistant and is considered to be the most immunodominant peptide within gluten in celiac disease (CD). GIP were detectable and quantifiable in very different kind of difficult to analyze food, revealing the potential immunogenicity by detecting T-cell activity of celiac patients. But GIP were also found in stool and urine of celiac patients on a supposedly gluten-free diet (GFD), showing the capacity to resist and be absorbed and excreted from the body, providing the first simple and objective means to assess adherence to the GFD. Methods to specifically and sensitively detect the most active GIP in food and biological fluids are rational candidates may use similar analytical standard references for determination of the immunopathological risk of gluten exposure in gluten-related diseases.
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Gluten Immunogenic Peptides as Standard for the Evaluation of Potential Harmful Prolamin Content in Food and Human Specimen
2018Co-Authors: Ángel Cebolla, María De Lourdes Moreno, Laura Coto, Carolina SousaAbstract:Gluten is a complex mixture of storage proteins in cereals like wheat, barley and rye. Prolamins are the main components of gluten. Their high content in proline and glutamine makes them water-insoluble and difficult to digest in the gastrointestinal tract. Partial digestion generates peptide sequences which trigger immune responses in celiac and gluten-sensitive patients. Gluten detection in food is challenging because of the diversity, in various food matrices, of protein proportions and their immunogenicity. Attempts to develop standard reference materials have been unsuccessful. We present here a summary of recent studies reporting the detection of dominant Gluten Immunogenic Peptides (GIP) sharing epitopes presented in the α-gliadin 33-mer, the most important celiac disease-immunogenic sequence within gluten. GIP were not only detectable and quantifiable in very different kind of difficult to analyze food, but also in stool and urine of celiac patients on a supposedly gluten-free diet (GFD), providing the first simple and objective means to assess adherence to the GFD. Methods to specifically and sensitively detect the most active GIP in food and biological fluids are rational candidates may use similar analytical standard references for determination of the immunopathological risk of gluten exposure in gluten-related diseases.
Peter Vock - One of the best experts on this subject based on the ideXlab platform.
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toward clinical x ray phase contrast ct demonstration of enhanced soft tissue contrast in Human Specimen
Investigative Radiology, 2010Co-Authors: Tilman Donath, Franz Pfeiffer, O Bunk, C Grunzweig, Eckhard Hempel, Stefan Popescu, Peter Vock, Christian DavidAbstract:X-ray computed tomography (CT) using phase contrast can provide images with greatly enhanced soft-tissue contrast in comparison to conventional attenuation-based CT. We report on the first scan of a Human Specimen recorded with a phase-contrast CT system based on an x-ray grating interferometer and a conventional x-ray tube source. Feasibility and potential applications of preclinical and clinical phase-contrast CT are discussed.
