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Achmad Subagio - One of the best experts on this subject based on the ideXlab platform.

  • Protein Rich Flour from Hyacinth Bean as Functional Food Ingredient with Low Glycemic Index
    Bogor Agricultural University; Indonesian Food Technologist Association (IAFT), 2013
    Co-Authors: Ahmad Nafi’, Wiwik Siti Windrati, Andri Pamungkas, Achmad Subagio
    Abstract:

    Protein-rich flour (PRF) produced from Hyacinth Bean (Lablab purpureus (L) Sweet) shows good potency as a functional food ingredient. The PRF was extracted from Hyacinth Bean using water followed by protein precipitation at its isoelectric point. The precipitate was neutralized using 1 N NaOH and the slurry was dried, ground and sieved. The objective of this research was to characterize the nutritive value of PRF i.e., protein content and amino acid profile, trypsin inhibitors activity, content of vitamins B1 and B2, the amylose and amylopectin ratio of starch and its glycemic index. The results showed that the PRF contained high protein (58.4±4.5%). The major amino acid was glutamic acid, while methionine was found as the limited amino acid of the PRF. The activity of trypsin inhibitor was low (20.4±1.6 unit/g). Moreover, PRF contains 0.2 and 3.6 mg/100 g of vitamins B1 and B2 respectively. With a high ratio of amylose (30.0±2.0%) and high content of resistance starch (7.97 g/100 g), the PRF showed a low glycemic index (43.50). Based on its characteristics, this PRF can be promoted as a new food ingredient, especially for diabetic diet

  • TEPUNG KAYA PROTEIN DARI KORO KOMAK SEBAGAI BAHAN PANGAN FUNGSIONAL BERINDEKS GLISEMIK RENDAH [Protein Rich Flour from Hyacinth Bean as Functional Food Ingredient with Low Glycemic Index]
    Jurnal Teknologi dan Industri Pangan, 2013
    Co-Authors: Ahmad Nafi, Wiwik Siti Windrati, Andri Pamungkas, Achmad Subagio
    Abstract:

    ABSTRACT Protein-rich flour (PRF) produced from Hyacinth Bean (Lablab purpureus (L) Sweet) shows good potency as a functional food ingredient. The PRF was extracted from Hyacinth Bean using water followed by protein precipitation at its isoelectric point. The precipitate was neutralized using 1 N NaOH and the slurry was dried, ground and sieved. The objective of this research was to characterize the nutritive value of PRF i.e., protein content and amino acid profile, trypsin inhibitors activity, content of vitamins B1 and B2, the amylose and amylopectin ratio of starch and its glycemic index. The results showed that the PRF contained high protein (58.4±4.5%). The major amino acid was glutamic acid, while methionine was found as the limited amino acid of the PRF. The activity of trypsin inhibitor was low (20.4±1.6 unit/g). Moreover, PRF contains 0.2 and 3.6 mg/100 g of vitamins B1 and B2 respectively. With a high ratio of amylose (30.0±2.0%) and high content of resistance starch (7.97 g/100 g), the PRF showed a low glycemic index (43.50). Based on its characteristics, this PRF can be promoted as a new food ingredient, especially for diabetic diet. Keywords: glycemic index, nutritive value, protein rich flour Komak (Lablab purpureus (L) Sweet)

  • effects of protein isolate from Hyacinth Beans lablab purpureus l sweet seeds on cake characteristics
    Food Science and Technology Research, 2008
    Co-Authors: Achmad Subagio, Naofumi Morita
    Abstract:

    Effects of protein isolate prepared from Hyacinth Bean (Lablab purpureus (L.) Sweet) seeds on cake characteristics were studied. The addition of up to 1% of the protein isolate from Hyacinth Bean seeds could improve the baking properties of the cake. The volume development and specific volume of the cake with 1% protein isolate were 206.0%, and 2.63 ml/g, whereas those of the control were 160.3% and 2.17 ml/g. Isolate additions of up to 1% softened the cake, as indicated by decreases in texture values, i.e., 184, 112, 100 and 89 g of force for the control, 0.5, 0.75 and 1.0% protein isolate, respectively. However, when more than 1% protein isolate was added, the cake quality tended to decrease compared with that at 1%. Moreover, the more protein isolate was added, the more vivid the colour and the lower the staling rate. Therefore, the protein isolate from Hyacinth Beans merits further assessment as a practical food additive.

  • characterization of Hyacinth Bean lablab purpureus l sweet seeds from indonesia and their protein isolate
    Food Chemistry, 2006
    Co-Authors: Achmad Subagio
    Abstract:

    Hyacinth Bean (Lablab purpureus (L.) sweet) seeds from Indonesia were characterized for the purposes of usage as a protein source. Protein isolate was prepared from the seeds using an isoelectric method, which was also used to characterize the physicochemical and functional properties. Hyacinth Bean seeds have a moderate concentration of protein (17.1 ± 1.5%), and low concentration of HCN (1.1 ± 0.1 mg/100 g). However, before using the seeds as food, some treatments are needed to reduce their anti-nutritional factors, since the contents of trypsin inhibitor and phytate are 0.15 ± 0.02 TIU/mg and 18.9 ± 0.2 mg/g, respectively. Using the isoelectric preparation, the yield of protein isolate was low (7.38 ± 0.2 g per 100 g of the seeds), but the protein isolate had good colour, neutral odour, high protein content (89.8 ± 0.82%), and low ash (2.97 ± 0.36%). The protein isolate also had good functional properties, such as solubility, foaming capacity, and emulsifying activity. However, the foaming and emulsifying stabilities were low.

  • Development of Protein Rich Flour (PRF) from Hyacinth Bean (Lablab purpureus (L) Sweet) and Lima Bean (Phaseolus lunatus)
    Jurnal Teknologi dan Industri Pangan, 2006
    Co-Authors: Ahmad Nafi, Tri Susanto, Achmad Subagio
    Abstract:

    With respect to the high content of carbohydrate and protein, Protein Rich Flour (PRF) were developped from non-oilseed legumes i.e. Hyacinth Bean (Lablab purpureus (L) Sweet) and lima Bean (Phaseolus lunatus) PRF. PRFs were prepared using water and NaOH solution (0.01N) as the extraction solvent. After precipitation in their isoelectric point (pHs) the PRFs produced were characterized to determine the potential practical applications. The results showed that PRF from Hyacinth Bean extracted by water was the best product with yield of 31.19%, protein content of 58.41A‚±4.45%, solubility of 82-100% and oil holding capacity being 93.92A‚±9.19. Moreover pepsin-digestibility of the Hyacinth Bean PRF was higher (8.29A‚±0.34%) than soyBean protein isolate (7.10A‚±0.37%) or casein (7.04A‚±0.14%). Based on their characteristics, PRF is regarded as potential to be developed as novel food ingredient. Key words: Non-oilseed Legumes, PRF, Characterization

Abd Elmoneim O. Elkhalifa - One of the best experts on this subject based on the ideXlab platform.

  • Proximate composition, minerals, tannins, in vitro protein digestibility and effect of cooking on protein fractions of Hyacinth Bean (Dolichos lablab)
    Journal of Food Science and Technology-mysore, 2002
    Co-Authors: Osama O. A. El Siddig, Abdullahi H. El Tinay, Abdel Wahab H. Abd Alla, Abd Elmoneim O. Elkhalifa
    Abstract:

    Four Hyacinth Bean cultivars (Dolichos lablab L.) were used in this study. Protein contents varied from 22.7-26.1%, fat contents 1.1-2.0%, moisture contents 7.8-8.1%, ash contents 3.8-4.3%, fibre contents 9.3-10.9% and carbohydrate contents 48.3-53.6%. Phosphorus values ranged from 305-312, magnesium : 167.4-231.1, sodium : 6-9 and potassium : 183.3-214 mg/100g. Tannin contents of the four Hyacinth Bean cultivars were in the range of 201-353 mg/100g, while the in vitro protein digestibility of the four cultivars was in the range 59.2-62.0%. The variation in protein fractions was : albumin 1.8-2.5%, globulin 69.9-81.2%, prolamin 2.7-4.4%, G 1 -glutelins 0.8-1.8%, G 2 -glutelins 1.1-3.0%, G 3 -glutelins 10.0-11.9% and insoluble proteins 0.2-9.9%. Two Hyacinth Bean cultivars, selected for their higher albumin and globulin fractions, were cooked in 150 ml of boiling distilled water under reflex. The protein fractions of the cooked seeds were determined. Results indicated that albumin and globulin fractions decreased significantly (p

  • proximate composition minerals tannins in vitro protein digestibility and effect of cooking on protein fractions of Hyacinth Bean dolichos lablab
    Journal of Food Science and Technology-mysore, 2002
    Co-Authors: Osama O. A. El Siddig, Abdullahi H. El Tinay, Abdel Wahab Abd H Alla, Abd Elmoneim O. Elkhalifa
    Abstract:

    Four Hyacinth Bean cultivars (Dolichos lablab L.) were used in this study. Protein contents varied from 22.7-26.1%, fat contents 1.1-2.0%, moisture contents 7.8-8.1%, ash contents 3.8-4.3%, fibre contents 9.3-10.9% and carbohydrate contents 48.3-53.6%. Phosphorus values ranged from 305-312, magnesium : 167.4-231.1, sodium : 6-9 and potassium : 183.3-214 mg/100g. Tannin contents of the four Hyacinth Bean cultivars were in the range of 201-353 mg/100g, while the in vitro protein digestibility of the four cultivars was in the range 59.2-62.0%. The variation in protein fractions was : albumin 1.8-2.5%, globulin 69.9-81.2%, prolamin 2.7-4.4%, G 1 -glutelins 0.8-1.8%, G 2 -glutelins 1.1-3.0%, G 3 -glutelins 10.0-11.9% and insoluble proteins 0.2-9.9%. Two Hyacinth Bean cultivars, selected for their higher albumin and globulin fractions, were cooked in 150 ml of boiling distilled water under reflex. The protein fractions of the cooked seeds were determined. Results indicated that albumin and globulin fractions decreased significantly (p<0.05) for both cultivars after cooking. This decrease was accompanied by a significant increase in prolamin, G 1 -glutelins, G 2 -glutelins and G 3 -glutelins fractions.

Filipa Monteiro - One of the best experts on this subject based on the ideXlab platform.

  • preliminary diversity assessment of an undervalued tropical Bean lablab purpureus l sweet through fatty acid profiling
    Plant Physiology and Biochemistry, 2018
    Co-Authors: Patricia Vidigal, Bernardo Duarte, Ana Rita Cavaco, Isabel Cacador, Andreia Figueiredo, Ana Rita Matos, Wanda Viegas, Filipa Monteiro
    Abstract:

    Several large-scale metabolic profiling studies have been directed to prospect crops with a major focus on yield-related traits and, ultimately, with the definition of specific markers for plant selection in breeding programs. However, some of these technologies are expensive, time-consuming and not easily feasible for a quick approach. Fatty acid profiling was described as reliable biomarkers and as a chemotaxonomic tool allowing to study not only the diversity in germplasm collections but also to discriminate their geographic origin. We have used fatty acids profiling for a preliminary assessment of Lablab purpureus (L.) Sweet (Hyacinth Bean) diversity and landraces discrimination. Hyacinth Bean displays an enormous variability of agro-morphological traits, probably linked to the multi-purpose uses in different regions, i.e. as pulse, or as food with nutraceutical potential (Africa and Asia), forage (Africa and Australia) and ornamental (Europe and USA). Only two forage cultivars are widely marketed, cv. Rongai and cv. Highworth, with several landraces remaining to be addressed in terms of diversity. We show that fatty acids profiling was able to distinguish landraces, which display shared fatty acids with cultivars from the center of Hyacinth Bean diversity origin (East Africa). We propose that fatty acid profiling is a tool that may be used not only for nutritional value assessment but also as a chemodiversity tool in crop research.

Shashi Pandey Rai - One of the best experts on this subject based on the ideXlab platform.

  • Recent advancement in modern genomic tools for adaptation of Lablab purpureus L to biotic and abiotic stresses: present mechanisms and future adaptations
    Acta Physiologiae Plantarum, 2018
    Co-Authors: Krishna Kumar Rai, Nagendra Rai, Shashi Pandey Rai
    Abstract:

    Hyacinth Bean is an important traditional plant with substantial medicinal value. Being imperative, it is still less explored crop on genomic and transcriptomic scale that has indexed it as an “orphan” crop for its genome revolution. Among different crop legumes such as pigeon pea, chickpea, cowpea, soyBean and common Bean, Hyacinth Bean also serves as a significant source of nutrition for both tropical and temperate regions and execute an imperative function in fixing biological nitrogen in agriculture. Nonetheless, the productivity of Hyacinth Bean is restrained due to environmental and biotic cues. Thus, understanding of the genomic functions and identification of probable genes/proteins for major agronomic traits through transcriptomic approaches has become imperative to improve stress tolerance in Hyacinth Bean. For understanding the plant stress tolerance mechanisms, the deployment of functional genomics approaches viz., proteomics and metabolomics have become imperious in breeding programs in developing countries. These approaches have been successfully used in other legume crops to create protein reference maps and their exploitation through comparative approaches can greatly enhance the research and understanding of Hyacinth Bean biological processes to changing environmental conditions. In this review, emerging epigenomics, proteomics, metabolomics and phenomics approaches and their achievements both in model/crop legumes are discussed. Additionally, the review also provides an overview of the applications of advanced proteomics, metabolomics and next-generation sequencing technologies in the discovery of candidate biomarkers for the development of agronomically refined Hyacinth Bean which may further ensure food and nutritional security under adverse climacteric conditions in developing countries.

  • Recent advancement in modern genomic tools for adaptation of Lablab purpureus L to biotic and abiotic stresses: present mechanisms and future adaptations
    Acta Physiologiae Plantarum Franciszek Górski Institute of Plant Physiology Polish Academy of Sciences Kraków, 2018
    Co-Authors: Nagendra Rai, Shashi Pandey Rai
    Abstract:

    Not AvailableHyacinth Bean is an important traditional plant with substantial medicinal value. Being imperative, it is still less explored crop on genomic and transcriptomic scale that has indexed it as an “orphan” crop for its genome revolution. Among different crop legumes such as pigeon pea, chickpea, cowpea, soyBean and common Bean, Hyacinth Bean also serves as a significant source of nutrition for both tropical and temperate regions and execute an imperative function in fixing biological nitrogen in agriculture. Nonetheless, the productivity of Hyacinth Bean is restrained due to environmental and biotic cues. Thus, understanding of the genomic functions and identification of probable genes/proteins for major agronomic traits through transcriptomic approaches has become imperative to improve stress tolerance in Hyacinth Bean. For understanding the plant stress tolerance mechanisms, the deployment of functional genomics approaches viz., proteomics and metabolomics have become imperious in breeding programs in developing countries. These approaches have been successfully used in other legume crops to create protein reference maps and their exploitation through comparative approaches can greatly enhance the research and understanding of Hyacinth Bean biological processes to changing environmental conditions. In this review, emerging epigenomics, proteomics, metabolomics and phenomics approaches and their achievements both in model/crop legumes are discussed. Additionally, the review also provides an overview of the applications of advanced proteomics, metabolomics and next-generation sequencing technologies in the discovery of candidate biomarkers for the development of agronomically refined Hyacinth Bean which may further ensure food and nutritional security under adverse climacteric conditions in developing countries.Not Availabl

  • salicylic acid and nitric oxide alleviate high temperature induced oxidative damage in lablab purpureus l plants by regulating bio physical processes and dna methylation
    Plant Physiology and Biochemistry, 2018
    Co-Authors: Krishna Kumar Rai, N Rai, Shashi Pandey Rai
    Abstract:

    Abstract Salicylic acid (SA) and sodium nitroprusside (SNP, NO donor) modulates plant growth and development processes and recent findings have also revealed their involvement in the regulation of epigenetic factors under stress condition. In the present study, some of these factors were comparatively studied in Hyacinth Bean plants subjected to high temperature (HT) environment (40–42 °C) with and without exogenous application of SA and SNP under field condition. Exogenous application of SA and SNP substantially modulated the growth and biophysical process of Hyacinth Bean plants under HT environment. Exogenous application of SA and SNP also remarkably regulated the activities of antioxidant enzymes, modulated mRNA level of certain enzymes, improves plant water relation, enhance photosynthesis and thereby increasing plant defence under HT. Coupled restriction enzyme digestion-random amplification (CRED-RA) technique revealed that many methylation changes were “dose dependent” and HT significantly increased DNA damages as evidenced by both increase and decrease in bands profiles, methylation and de-methylation pattern. Thus, the result of the present study clearly shows that exogenous SA and SNP regulates DNA methylation pattern, modulates stress-responsive genes and can impart transient HT tolerance by synchronizing growth and physiological acclimatization of plants, thus narrowing the gaps between physio-biochemical and molecular events in addressing HT tolerance.

V R Devaraj - One of the best experts on this subject based on the ideXlab platform.

  • Role of exogenous proline in improving water and antioxidant status in Hyacinth Bean subjected to salt stress
    2014
    Co-Authors: V R Devaraj
    Abstract:

    Abstract Proline is an important component of salt stress response in plants. In this study the role of proline in facilitating seed germination and in alleviating salt stress in Hyacinth Bean (Lablab purpureus) seedlings was examined. The results showed that 2 mM proline stimulated a better germination percentage in NaCl-treated seeds when compared to untreated seeds. Effect of salinity on Hyacinth Bean was also evaluated in 10-day old seedlings with 100 – 500 mM NaCl over 72 h of exposure. Leaf RWC, endogenous proline, H 2 O 2 , reduced glutathione, MDA, ascorbate, total phenols, total soluble sugar contents as well as catalase, guaiacol peroxidase and glutathione reductase were determined after 72 h of salt treatment. The results showed that salt stress significantly decreased leaf RWC and CAT activity and increased MDA, glutathione and ascorbate contents and POX and GR activities particularly at 300 mM NaCl. The exogenous application of proline (5 mM) significantly alleviated the NaCl induced growth inhibition as noted by lower MDA and higher RWC, POX and GR activity. The CAT activity was less inhibited in the presence of exogenous proline. Thus, addition of exogenous proline to the hydroponics enhances salt tolerance of Lablab purpureus by improving water status and CAT activity in the plant.

  • oxidative stress biomarkers and metabolic changes associated with cadmium stress in Hyacinth Bean lablab purpureus
    African Journal of Biotechnology, 2013
    Co-Authors: Myrene R Dsouza, V R Devaraj
    Abstract:

    Cadmium (Cd)-induced stress in Hyacinth Bean ( Lablab purpureus ) was investigated by growing seedlings in a nutrient solution containing increasing cadmium concentrations (0 to 50 μM), under strictly controlled growth conditions. Changes consequent to Cd uptake in growth parameters, enzyme activities and other stress response factors directly or indirectly are related to the cellular free radical scavenging systems. Antioxidants and other stress response components were studied in roots and leaves of 10-day old seedlings over 72 h of exposure. A significant decrease in length, relative water content (RWC), weight of shoot and root, and chlorophyll content was observed in seedlings growing on >10 μM CdCl 2 . Oxidative stress markers, H 2 O 2 , malondialdehyde, proline and total soluble sugars were elevated in both leaves and roots. Ascorbate and phenol contents increased in leaves, but, decreased in roots. Reduced glutathione levels declined in both tissues. Cd stress enhanced antioxidant enzymes, guaiacol peroxidase, glutathione reductase, ascorbate peroxidase and polyphenol oxidase in concentration and time dependent manner in leaves. Antioxidant enzymes in roots showed inverse relationship with concentration and time of exposure. Catalase activity was found to decrease in both leaves and roots. Metabolic enzymes β-amylase and acid phosphatase activity increased in both leaves and roots. The results suggest that primary antioxidative response originates from leaves of Hyacinth Bean even though roots are involved in direct uptake of heavy metals. The root tends to accumulate Cd and thus excludes its uptake by leaves. Keywords : Antioxidants, antioxidant enzymes, cadmium, Hyacinth Bean. African Journal of Biotechnology Vol. 12(29), pp. 4670-4682

  • Induction of oxidative stress and antioxidative mechanisms in Hyacinth Bean under zinc stress.
    African Crop Science Journal, 2012
    Co-Authors: R Myrene D’souza, V R Devaraj
    Abstract:

    Zinc toxicity to plants occurs in soils contaminated by mining and smelting activities, in agricultural soils treated with sewage sludge, and in urban and peri-urban soils enriched by anthropogenic inputs of Zn. Zinc-induced stress in Hyacinth Bean ( Lablab purpureus ) was investigated by growing seedlings in hydroponics, supplemented with Zinc (0-600 μM), under controlled growth conditions. Changes in growth parameters, enzyme activities and other stress response factors, as a function of Zn uptake are directly or indirectly related to the cellular free radical scavenging systems. These parameters were studied in 10-day old Lablab seedlings over 72 h of exposure. Relative to leaves, roots accumulated ~12-fold higher amounts of Zn. Oxidative stress markers, H2O2, malondialdehyde, ascorbate, proline, soluble sugars, putrescine and spermidine were elevated in both tissues, whereas, glutathione and spermine levels showed a steady decline. Total phenol increased in roots, but decreased in leaves. Zn-stress enhanced antioxidant enzymes, guaiacol peroxidase, glutathione reductase, and polyphenol oxidase in concentration and time dependent manner in leaves, but showed an inverse relationship in roots. Catalase decreased in both leaves and roots. Metabolic enzymes β-amylase and acid phosphatase increased in both tissues. Invertase increased in leaves but declined drastically in roots. The results suggest that primary antioxidative response originates in leaves of Lablab, while roots are involved in direct uptake of heavy metals and are not adept in extrusion. Also, lower concentrations of Zn (up to 100 μM) stimulate growth of Lablab , but higher concentrations proved detrimental to the plant. Keywords: Lablab purpureus, Malondialdehyde, Polyamines African Crop Science Journal , Vol. 20, No. 1, pp. 17 - 29

  • Specific and non-specific responses of Hyacinth Bean (Dolichos lablab) to drought stress
    2011
    Co-Authors: Myrene R D’souza, V R Devaraj
    Abstract:

    Drought and salinity stress are the major causes of historic and modern agricultural productivity losses throughout the world. The availability of irrigation water is a challenge for many countries that have scarce water resources, yet are highly dependent on agriculture as a means of revenue. Effect of drought on Hyacinth Bean, Dolichos lablab (HA-​4 cultivar) was evaluated in 10-​d-​old seedlings for 8 d after withholding water. The stress reduced dry and fresh wt., leaf no., surface area, root and shoot length, total chlorophyll and relative water content. Oxidative stress markers, H2O2, glutathione, malondialdehyde, proline, ascorbic acid, total phenols, and total sol. sugars were significantly elevated. Drought enhanced antioxidant enzymes, peroxidase and glutathione reductase, and reduced catalase in a time dependent manner in the leaves. POX and CAT in roots showed inverse relationship with the duration of stress, whereas GR exhibited increased activity. The metabolic activity of enzymes, β-​amylase and acid phosphatase, increased temporally in leaves and roots. Intensity of isoenzymes correlated with in vitro levels under stress. The plant showed ability to rehydrate and grow upon re-​watering, and levels of antioxidant components correlated with drought tolerance of the plant.

  • biochemical responses of Hyacinth Bean lablab purpureus to salinity stress
    Acta Physiologiae Plantarum, 2010
    Co-Authors: Myrene R Dsouza, V R Devaraj
    Abstract:

    Effect of salinity on Hyacinth Bean, Lablab purpureus (HA-4 cultivar) was evaluated in 10-day old seedlings with 100–500 mM NaCl over 72 h of exposure. The stress reduced dry and fresh weight, leaf surface area, root and shoot length, total chlorophyll, and RWC. Oxidative stress markers, H2O2, glutathione, TBARS, proline, ascorbic acid, total phenols, and total soluble sugar contents were significantly elevated. Salinity enhanced antioxidant enzymes, POX, and GR activities and reduced that of CAT in concentration and time dependent manner in leaves. Antioxidant enzymes in roots showed inverse relationship with concentration and time of exposure. Metabolic enzyme β-amylase activity increased in both leaves and roots. Acid phosphatase decreased in leaves and elevated in roots. Intensity of constitutive isozymes correlated with in vitro levels under stress, but the protein band patterns differed from controls. Lablab showed reasonable tolerance up to 300 mM NaCl, but leaves and roots differed in their response.