The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform
Ze Chen - One of the best experts on this subject based on the ideXlab platform.
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microrna let 7 regulates the expression of ecdysteroid receptor ecr in Hyalomma asiaticum acari ixodidae ticks
Parasites & Vectors, 2019Co-Authors: Feng Wu, Ze Chen, Ronghai Xiao, Zhiqiang Qu, Zegong Wu, Hui WangAbstract:Ticks are blood-sucking arthropods that can transmit diseases to humans and animals. These arthropods are the second most important vectors of pathogens. MicroRNAs are a class of conserved small noncoding RNAs that play regulatory roles in gene expression at the post-transcriptional level. Molting is an important biological process in arthropods. Research on the molting process is important for understanding tick physiology and control. Dual-luciferase reporter assays were used to assess the role of miRNA let-7 in ecdysteroid receptor (ECR) biology. The expression levels of ECR and let-7 were measured by real-time qPCR before and after tick molting. To explore the function of let-7 and ECR, we performed overexpression and knocking down of let-7 and RNAi of ECR in tick nymphs. The biological function of let-7 in molting was explored by injecting nymphs, ten days after engorgement, with let-7 agomir for overexpression and let-7 antagomir for knocking down. The rate of molting was then determined. ECR dsRNA was injected into ticks to evaluate the function of ECR by gene silencing. The expression of ECR and let-7 was measured using RT-qPCR. All data were analyzed using GraphPad Prism v.6. The results of the luciferase assay using a eukaryotic expression system revealed that ECR was a natural target of let-7. Let-7 overexpressed by agomir affected the rate of molting (P < 0.01) and the period of molting (P < 0.01). Let-7 antagomir for knockdown affected the period of molting (P < 0.01), but there was no effect on the rate of molting (P = 0.27). ECR dsRNA gene silencing significantly affected the rate of molting (P < 0.05). This study demonstrated that let-7 can regulate the expression of ECR and that let-7 can affect molting in ticks. Our results help to understand the regulation of let-7 by 20-hydroxyecdysone (20E) and will provide a reference for functional analysis studies of microRNAs in ticks.
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MicroRNA let-7 regulates the expression of ecdysteroid receptor (ECR) in Hyalomma asiaticum (Acari: Ixodidae) ticks
Parasites & Vectors, 2019Co-Authors: Feng Wu, Ze Chen, Ronghai Xiao, Zhiqiang QuAbstract:Ticks are blood-sucking arthropods that can transmit diseases to humans and animals. These arthropods are the second most important vectors of pathogens. MicroRNAs are a class of conserved small noncoding RNAs that play regulatory roles in gene expression at the post-transcriptional level. Molting is an important biological process in arthropods. Research on the molting process is important for understanding tick physiology and control. Dual-luciferase reporter assays were used to assess the role of miRNA let-7 in ecdysteroid receptor (ECR) biology. The expression levels of ECR and let-7 were measured by real-time qPCR before and after tick molting. To explore the function of let-7 and ECR, we performed overexpression and knocking down of let-7 and RNAi of ECR in tick nymphs. The biological function of let-7 in molting was explored by injecting nymphs, ten days after engorgement, with let-7 agomir for overexpression and let-7 antagomir for knocking down. The rate of molting was then determined. ECR dsRNA was injected into ticks to evaluate the function of ECR by gene silencing. The expression of ECR and let-7 was measured using RT-qPCR. All data were analyzed using GraphPad Prism v.6. The results of the luciferase assay using a eukaryotic expression system revealed that ECR was a natural target of let-7. Let-7 overexpressed by agomir affected the rate of molting (P
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morphological characteristics of normal and gynandromorphic Hyalomma asiaticum schulze and schlottke 1930
Korean Journal of Parasitology, 2015Co-Authors: Ze Chen, Youquan Li, Yonghong Hu, Kai LiAbstract:Gynandromorphic ticks are extremely rare, and often attract parasitologists’ attention. During our examination of tick specimens, an engorged gynandromorph of Hyalomma asiaticum was noticed. This is the first record of gynandromorphic ticks from China. In this study, several important morphological structures of normal and gynandromorphic H. asiaticum were analyzed. Comparing to the normal H. asiaticum, the gynandromorphic specimen was a typical bipartite protogynander. Its right side showed normal female characteristics, whereas the left side had normal male traits. Different from other gynandromorphic ticks containing 1 anus, this tick reported here had 2 complete anuses, and the anus of the male part had a single adanal plate.
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Ticks (Acari: Ixodoidea: Argasidae, Ixodidae) of China
Experimental and Applied Acarology, 2010Co-Authors: Ze Chen, Xiaojun Yang, Fengju Bu, Xiaohong Yang, Xiaolong YangAbstract:This paper presents results of an investigation and listing of tick species found in China during a survey in all 28 provinces. This will be a step towards a definitive list of tick species and their distribution. To date, the tick fauna of this area consists of 117 species in the following families: Argasidae-Argas (7 species), Carios (4 species) and Ornithodoros (2 species); Ixodidae-Amblyomma (8 species), Anomalohimalaya (2 species), Dermacentor (12 species), Haemaphysalis (44 species), Hyalomma (6 species), Ixodes (24 species) and Rhipicephalus (8 species). Some well known ticks carrying and transmitting many infectious agents to man and domestic animals are also found in China. These include Ixodes persulcatus, Haemaphysalis longicornis, Rhipicephalus sanguineus, R. (Boophilus) microplus and Hyalomma asiaticum. It is worth mentioning that Ixodes rangtangensis Teng and Haemaphysalis xinjiangensis Teng should be relegated to a synonym of I. moschiferi and Hae. danieli, respectively. The distribution of ticks over the provinces of China is also discussed. The information on ticks in some areas such as Henan is not exhaustive.
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the life cycle of Hyalomma asiaticum kozlovi olenev 1931 acari ixodidae under laboratory conditions
Veterinary Parasitology, 2009Co-Authors: Ze Chen, Zhijun Yu, Xiaojun Yang, Hongyuan ZhengAbstract:Abstract The developmental stages in the life cycle of Hyalomma asiaticum kozlovi were investigated under laboratory conditions. The larval, nymphal and adult ticks were all fed on rabbits at 25–27 °C, 50% relative humidity (RH) and exposed to daylight. All free-living stages were maintained in an incubator at 26 ± 1 °C, 70% RH and daylight conditions. The life cycle of H. asiaticum kozlovi was completed in an average period of 151.6 days (range 104–190). The average developmental periods were 38.8 days for egg incubation, 2.1, 5.8 and 10.7 days for larval prefeeding, feeding and premoulting, and 3.3, 6.0 and 26.0 days for the same stages of nymphs, respectively. The average times for prefeeding, feeding, preoviposition and oviposition of female adult ticks were 1.4, 10.1, 20.9, and 26.5 days, respectively. The results confirmed the significant correlation between the weight of the engorged female and the amount of the egg mass laid ( r = 0.9641, p
Hongyuan Zheng - One of the best experts on this subject based on the ideXlab platform.
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the life cycle of Hyalomma asiaticum kozlovi olenev 1931 acari ixodidae under laboratory conditions
Veterinary Parasitology, 2009Co-Authors: Ze Chen, Zhijun Yu, Xiaojun Yang, Hongyuan ZhengAbstract:Abstract The developmental stages in the life cycle of Hyalomma asiaticum kozlovi were investigated under laboratory conditions. The larval, nymphal and adult ticks were all fed on rabbits at 25–27 °C, 50% relative humidity (RH) and exposed to daylight. All free-living stages were maintained in an incubator at 26 ± 1 °C, 70% RH and daylight conditions. The life cycle of H. asiaticum kozlovi was completed in an average period of 151.6 days (range 104–190). The average developmental periods were 38.8 days for egg incubation, 2.1, 5.8 and 10.7 days for larval prefeeding, feeding and premoulting, and 3.3, 6.0 and 26.0 days for the same stages of nymphs, respectively. The average times for prefeeding, feeding, preoviposition and oviposition of female adult ticks were 1.4, 10.1, 20.9, and 26.5 days, respectively. The results confirmed the significant correlation between the weight of the engorged female and the amount of the egg mass laid ( r = 0.9641, p
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The life cycle of Hyalomma asiaticum kozlovi Olenev, 1931 (Acari: Ixodidae) under laboratory conditions.
Veterinary parasitology, 2008Co-Authors: Ze Chen, Zhijun Yu, Xiaojun Yang, Hongyuan ZhengAbstract:The developmental stages in the life cycle of Hyalomma asiaticum kozlovi were investigated under laboratory conditions. The larval, nymphal and adult ticks were all fed on rabbits at 25-27 degrees C, 50% relative humidity (RH) and exposed to daylight. All free-living stages were maintained in an incubator at 26+/-1 degrees C, 70% RH and daylight conditions. The life cycle of H. asiaticum kozlovi was completed in an average period of 151.6 days (range 104-190). The average developmental periods were 38.8 days for egg incubation, 2.1, 5.8 and 10.7 days for larval prefeeding, feeding and premoulting, and 3.3, 6.0 and 26.0 days for the same stages of nymphs, respectively. The average times for prefeeding, feeding, preoviposition and oviposition of female adult ticks were 1.4, 10.1, 20.9, and 26.5 days, respectively. The results confirmed the significant correlation between the weight of the engorged female and the amount of the egg mass laid (r=0.9641, p
Da Yu - One of the best experts on this subject based on the ideXlab platform.
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a tick b cell inhibitory protein from salivary glands of the hard tick Hyalomma asiaticum asiaticum
Biochemical and Biophysical Research Communications, 2006Co-Authors: Da Yu, Jiangguo Liang, Haining Yu, Haifeng Wu, Chunhua XuAbstract:Some studies done to date suggest that B-cell inhibitory factor occurred in tick saliva. In this study, a novel protein having B-cell inhibitory activity was purified and characterized from the salivary glands of the hard tick, Hyalomma asiaticum asiaticum. This protein was named B-cell inhibitory factor (BIF). The cDNA encoding BIF was cloned by cDNA library screening. The predicted protein from the cDNA sequence is composed of 138 amino acids including the mature BIF. No similarity was found by Blast search. The lipopolysaccharide-induced B-cell proliferation was inhibited by BIF. This is the first report of the identification and characterization of B-cell inhibitory protein from tick. The current study facilitates the study of identifying the interaction among tick, Borrelia burgdorferi, the causative agent of Lyme disease, and host.
Hui Wang - One of the best experts on this subject based on the ideXlab platform.
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microrna let 7 regulates the expression of ecdysteroid receptor ecr in Hyalomma asiaticum acari ixodidae ticks
Parasites & Vectors, 2019Co-Authors: Feng Wu, Ze Chen, Ronghai Xiao, Zhiqiang Qu, Zegong Wu, Hui WangAbstract:Ticks are blood-sucking arthropods that can transmit diseases to humans and animals. These arthropods are the second most important vectors of pathogens. MicroRNAs are a class of conserved small noncoding RNAs that play regulatory roles in gene expression at the post-transcriptional level. Molting is an important biological process in arthropods. Research on the molting process is important for understanding tick physiology and control. Dual-luciferase reporter assays were used to assess the role of miRNA let-7 in ecdysteroid receptor (ECR) biology. The expression levels of ECR and let-7 were measured by real-time qPCR before and after tick molting. To explore the function of let-7 and ECR, we performed overexpression and knocking down of let-7 and RNAi of ECR in tick nymphs. The biological function of let-7 in molting was explored by injecting nymphs, ten days after engorgement, with let-7 agomir for overexpression and let-7 antagomir for knocking down. The rate of molting was then determined. ECR dsRNA was injected into ticks to evaluate the function of ECR by gene silencing. The expression of ECR and let-7 was measured using RT-qPCR. All data were analyzed using GraphPad Prism v.6. The results of the luciferase assay using a eukaryotic expression system revealed that ECR was a natural target of let-7. Let-7 overexpressed by agomir affected the rate of molting (P < 0.01) and the period of molting (P < 0.01). Let-7 antagomir for knockdown affected the period of molting (P < 0.01), but there was no effect on the rate of molting (P = 0.27). ECR dsRNA gene silencing significantly affected the rate of molting (P < 0.05). This study demonstrated that let-7 can regulate the expression of ECR and that let-7 can affect molting in ticks. Our results help to understand the regulation of let-7 by 20-hydroxyecdysone (20E) and will provide a reference for functional analysis studies of microRNAs in ticks.
Zhijun Yu - One of the best experts on this subject based on the ideXlab platform.
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Antibacterial Activity of Eggs and Egg Wax Covering of Selected Ixodid (Acari: Ixodidae) Ticks
Journal of Entomological Science, 2017Co-Authors: Xiaolong Yang, Jie Chen, Shiyong Zhai, Zhijun YuAbstract:Abstract Antimicrobial activity of eggs and the wax covering of eggs has been observed in several tick (Acari) species, but any antimicrobial activity associated with ixodid (Acari: Ixodidae) tick eggs is unknown. The antimicrobial activity associated with eggs, wax extracts from eggs, and eggs from which wax was removed of the ixodids Haemaphysalis longicornis Neumann (parthenogenetic and bisexual populations), Haemaphysalis doenitzi Warburton et Nuttal, Dermacentor silvarum Olenev, and Hyalomma asiaticum Schulze and Schlottke was assessed using an in vitro agar diffusion assay. Eggs of D. silvarum and Hy. asiaticum placed on the agar culture inhibited the growth of gram-negative bacteria but had no activity against gram-positive bacteria. Eggs of H. longicornis (parthenogenesis and bisexual population) and H. doenitzi inhibited the growth of gram-positive bacteria, but not gram-negative bacteria. All eggs from which the wax had been extracted had no activity against either gram-positive or gram-negative...
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the life cycle of Hyalomma asiaticum kozlovi olenev 1931 acari ixodidae under laboratory conditions
Veterinary Parasitology, 2009Co-Authors: Ze Chen, Zhijun Yu, Xiaojun Yang, Hongyuan ZhengAbstract:Abstract The developmental stages in the life cycle of Hyalomma asiaticum kozlovi were investigated under laboratory conditions. The larval, nymphal and adult ticks were all fed on rabbits at 25–27 °C, 50% relative humidity (RH) and exposed to daylight. All free-living stages were maintained in an incubator at 26 ± 1 °C, 70% RH and daylight conditions. The life cycle of H. asiaticum kozlovi was completed in an average period of 151.6 days (range 104–190). The average developmental periods were 38.8 days for egg incubation, 2.1, 5.8 and 10.7 days for larval prefeeding, feeding and premoulting, and 3.3, 6.0 and 26.0 days for the same stages of nymphs, respectively. The average times for prefeeding, feeding, preoviposition and oviposition of female adult ticks were 1.4, 10.1, 20.9, and 26.5 days, respectively. The results confirmed the significant correlation between the weight of the engorged female and the amount of the egg mass laid ( r = 0.9641, p
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The life cycle of Hyalomma asiaticum kozlovi Olenev, 1931 (Acari: Ixodidae) under laboratory conditions.
Veterinary parasitology, 2008Co-Authors: Ze Chen, Zhijun Yu, Xiaojun Yang, Hongyuan ZhengAbstract:The developmental stages in the life cycle of Hyalomma asiaticum kozlovi were investigated under laboratory conditions. The larval, nymphal and adult ticks were all fed on rabbits at 25-27 degrees C, 50% relative humidity (RH) and exposed to daylight. All free-living stages were maintained in an incubator at 26+/-1 degrees C, 70% RH and daylight conditions. The life cycle of H. asiaticum kozlovi was completed in an average period of 151.6 days (range 104-190). The average developmental periods were 38.8 days for egg incubation, 2.1, 5.8 and 10.7 days for larval prefeeding, feeding and premoulting, and 3.3, 6.0 and 26.0 days for the same stages of nymphs, respectively. The average times for prefeeding, feeding, preoviposition and oviposition of female adult ticks were 1.4, 10.1, 20.9, and 26.5 days, respectively. The results confirmed the significant correlation between the weight of the engorged female and the amount of the egg mass laid (r=0.9641, p
