The Experts below are selected from a list of 114327 Experts worldwide ranked by ideXlab platform
Hongyuan Chen - One of the best experts on this subject based on the ideXlab platform.
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gold nanoparticle colloidal carbon nanosphere Hybrid Material preparation characterization and application for an amplified electrochemical immunoassay
Advanced Functional Materials, 2008Co-Authors: Jianming Shen, Hongyuan ChenAbstract:A rapid microwave-hydrothermal method has been developed to prepare monodisperse colloidal carbon nanospheres from glucose solution, and gold nanoparticles (AuNPs) are successfully assembled on the surface of the colloidal carbon nanospheres by a self-assembly approach. The resulting AuNP/colloidal carbon nanosphere Hybrid Material (AuNP/C) has been characterized and is expected to offer a promising template for biomolecule immobilization and biosensor fabrication because of its satisfactory chemical stability and the good biocompatibility of AuNPs. Herein, as an example, it is demonstrated that the as-prepared AuNP/C Hybrid Material can be conjugated with horseradish peroxidase-labeled antibody (HRP-Ab2) to fabricate HRP-Ab2-AuNP/C bioconjugates, which can then be used as a label for the sensitive detection of protein. The amperometric immunosensor fabricated on a carbon nanotube-modified glass carbon electrode was very effective for antibody immobilization. The approach provided a linear response range between 0.01 and 250 ng mL−1 with a detection limit of 5.6 pg mL−1. The developed assay method was versatile, offered enhanced performances, and could be easily extended to other protein detection as well as DNA analysis.
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porous gold nanoparticle caco3 Hybrid Material preparation characterization and application for horseradish peroxidase assembly and direct electrochemistry
Chemistry of Materials, 2006Co-Authors: Wenyi Cai, Xiaoning Zhao, Junjie Zhu And, Hongyuan ChenAbstract:Gold nanoparticles (AuNPs) were assembled on the surface of porous calcium carbonate microspheres (CaCO3) in a neutral aqueous solution through electrostatic interaction. The resulting gold-nanoparticle−CaCO3 Hybrid Material (AuNP−CaCO3) was characterized and expected to offer a promising template for enzyme immobilization and biosensor fabrication because of its satisfying biocompatibility and improved properties. It was conjugated with horseradish peroxidase (HRP) to fabricate HRP−AuNP−CaCO3 bioconjugates, which were then embedded into a silica sol−gel matrix to construct a novel biosensor. Because of the synergic effect of CaCO3 microspheres and AuNPs, direct electron transfer of HRP was observed at this biosensor. The biosensor exhibited a fast amperometric response to H2O2, with a linear range of 4.0 × 10-5 to 8.0 × 10-3 mol/L. The detection limit was 1.0 × 10-6 mol/L based on S/N = 3.
Rongjing Cui - One of the best experts on this subject based on the ideXlab platform.
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fabrication of a novel electrochemical immunosensor based on the gold nanoparticles colloidal carbon nanosphere Hybrid Material
Electrochimica Acta, 2010Co-Authors: Rongjing Cui, Junjie ZhuAbstract:The gold nanoparticles/colloidal carbon sphere Hybrid Material was used for the immobilization of protein, and was developed in biosensing. The Hybrid Material was fabricated by the assembly of gold nanoparticles onto the surface of colloidal carbon spheres, which constructed a 3D antibody immobilization matrix on the glass carbon electrode and made the immobilized biomolecules hold high stability and bioactivity. After the sandwich-type immunoreaction, the formed HRP-labeled immunoconjugate showed good enzymatic activity for the oxidation of o-phenylenediamine by H2O2. The approach provided a linear response range between 5 and 250 ng/mL with a detection limit of 1.8 ng/mL. The immunosensor showed good precision, acceptable stability and reproducibility and could be used for the detection of human IgG in real samples, which provided a potential alternative tool for the detection of protein in clinical laboratory.
Junjie Zhu - One of the best experts on this subject based on the ideXlab platform.
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fabrication of a novel electrochemical immunosensor based on the gold nanoparticles colloidal carbon nanosphere Hybrid Material
Electrochimica Acta, 2010Co-Authors: Rongjing Cui, Junjie ZhuAbstract:The gold nanoparticles/colloidal carbon sphere Hybrid Material was used for the immobilization of protein, and was developed in biosensing. The Hybrid Material was fabricated by the assembly of gold nanoparticles onto the surface of colloidal carbon spheres, which constructed a 3D antibody immobilization matrix on the glass carbon electrode and made the immobilized biomolecules hold high stability and bioactivity. After the sandwich-type immunoreaction, the formed HRP-labeled immunoconjugate showed good enzymatic activity for the oxidation of o-phenylenediamine by H2O2. The approach provided a linear response range between 5 and 250 ng/mL with a detection limit of 1.8 ng/mL. The immunosensor showed good precision, acceptable stability and reproducibility and could be used for the detection of human IgG in real samples, which provided a potential alternative tool for the detection of protein in clinical laboratory.
Zhichao Pei - One of the best experts on this subject based on the ideXlab platform.
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a supramolecular Hybrid Material constructed from pillar 6 arene based host guest complexation and zif 8 for targeted drug delivery
Chemical Communications, 2018Co-Authors: Kui Yang, Ke Yang, Shuang Chao, Jia Wen, Yuxin Pei, Zhichao PeiAbstract:A novel supramolecular Hybrid Material ZIF-8@DOX@WP6@G constructed from the host-guest complexation between carboxylated pillar[6]arene (WP6) and a galactose derivative (G), and doxorubicin (DOX)-loaded ZIF-8 has been synthesized for targeted drug delivery. The results showed that ZIF-8@DOX@WP6@G not only maintained the pH-sensitive drug release properties of ZIF-8 but also exhibited excellent water dispersibility and selective toxicity for hepatoma cancer cells due to the assembly of WP6 and G. The strategy used in this study opens up a new avenue for constructing multifunctional supramolecular Hybrid Materials for therapeutic applications in cancer treatment.
Jianming Shen - One of the best experts on this subject based on the ideXlab platform.
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gold nanoparticle colloidal carbon nanosphere Hybrid Material preparation characterization and application for an amplified electrochemical immunoassay
Advanced Functional Materials, 2008Co-Authors: Jianming Shen, Hongyuan ChenAbstract:A rapid microwave-hydrothermal method has been developed to prepare monodisperse colloidal carbon nanospheres from glucose solution, and gold nanoparticles (AuNPs) are successfully assembled on the surface of the colloidal carbon nanospheres by a self-assembly approach. The resulting AuNP/colloidal carbon nanosphere Hybrid Material (AuNP/C) has been characterized and is expected to offer a promising template for biomolecule immobilization and biosensor fabrication because of its satisfactory chemical stability and the good biocompatibility of AuNPs. Herein, as an example, it is demonstrated that the as-prepared AuNP/C Hybrid Material can be conjugated with horseradish peroxidase-labeled antibody (HRP-Ab2) to fabricate HRP-Ab2-AuNP/C bioconjugates, which can then be used as a label for the sensitive detection of protein. The amperometric immunosensor fabricated on a carbon nanotube-modified glass carbon electrode was very effective for antibody immobilization. The approach provided a linear response range between 0.01 and 250 ng mL−1 with a detection limit of 5.6 pg mL−1. The developed assay method was versatile, offered enhanced performances, and could be easily extended to other protein detection as well as DNA analysis.
