The Experts below are selected from a list of 3819 Experts worldwide ranked by ideXlab platform
Tomoaki Nishida - One of the best experts on this subject based on the ideXlab platform.
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role of 1 Hydroxybenzotriazole in oxidation by laccase from trametes versicolor kinetic analysis of the laccase 1 Hydroxybenzotriazole couple
Fems Microbiology Letters, 2006Co-Authors: Hirofumi Hirai, Shingo Kawai, Hiroshi Shibata, Tomoaki NishidaAbstract:In the current studies, we used Lineweaver-Burke analysis to examine the role of 1-Hydroxybenzotriazole (HBT) in the oxidation of various compounds by laccase from Trametes versicolor. At low concentrations, HBT was a competitive inhibitor of the oxidation, but at high concentrations, it was a noncompetitive inhibitor. Analysis of the oxidation of ferrocytochrome c by the laccase-HBT couple showed that increasing the concentration of ferrocytochrome c did not affect the V(max) but reduced the apparent K(m). In addition, in the manganese peroxidase-Mn(II) reaction, which is a typical oxidation system by mediator, the apparent K(m) and V(max) increased as the concentration of the substrate 2,6-dimethoxyphenol was increased. These results indicate that HBT is involved in the binding of laccase and substrates that laccase cannot oxidize alone.
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Role of 1‐Hydroxybenzotriazole in oxidation by laccase from Trametes versicolor. Kinetic analysis of the laccase‐1‐Hydroxybenzotriazole couple
Fems Microbiology Letters, 2006Co-Authors: Hirofumi Hirai, Shingo Kawai, Hiroshi Shibata, Tomoaki NishidaAbstract:In the current studies, we used Lineweaver-Burke analysis to examine the role of 1-Hydroxybenzotriazole (HBT) in the oxidation of various compounds by laccase from Trametes versicolor. At low concentrations, HBT was a competitive inhibitor of the oxidation, but at high concentrations, it was a noncompetitive inhibitor. Analysis of the oxidation of ferrocytochrome c by the laccase-HBT couple showed that increasing the concentration of ferrocytochrome c did not affect the V(max) but reduced the apparent K(m). In addition, in the manganese peroxidase-Mn(II) reaction, which is a typical oxidation system by mediator, the apparent K(m) and V(max) increased as the concentration of the substrate 2,6-dimethoxyphenol was increased. These results indicate that HBT is involved in the binding of laccase and substrates that laccase cannot oxidize alone.
Emanuele Ussia - One of the best experts on this subject based on the ideXlab platform.
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Oxidative cleavage of 1-aryl-isochroman derivatives using the Trametes villosa laccase/1-Hydroxybenzotriazole system
New Journal of Chemistry, 2016Co-Authors: Roberta Bernini, Patrizia Gentili, Fernanda Crisante, Francesca D'acunzo, Emanuele UssiaAbstract:The oxidative cleavage of the dihydropyran ring of 1-aryl-isochroman derivatives was carried out for the first time under green chemistry conditions in the presence of the Trametes villosa laccase/1-Hydroxybenzotriazole system in buffered water/1,4-dioxane and buffered water/dimethyl carbonate as reaction media. The corresponding oxidation products [2-(2-hydroxyethyl)benzophenone derivatives] were obtained in different yields depending on the substituents on phenyl and isochroman rings. These compounds are useful intermediates for the synthesis of anticancer agents and neuroprotective drugs.
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oxidative cleavage of 1 aryl isochroman derivatives using the trametes villosa laccase 1 Hydroxybenzotriazole system
New Journal of Chemistry, 2016Co-Authors: Roberta Bernini, Patrizia Gentili, Fernanda Crisante, Francesca Dacunzo, Emanuele UssiaAbstract:The oxidative cleavage of the dihydropyran ring of 1-aryl-isochroman derivatives was carried out for the first time under green chemistry conditions in the presence of the Trametes villosa laccase/1-Hydroxybenzotriazole system in buffered water/1,4-dioxane and buffered water/dimethyl carbonate as reaction media. The corresponding oxidation products [2-(2-hydroxyethyl)benzophenone derivatives] were obtained in different yields depending on the substituents on phenyl and isochroman rings. These compounds are useful intermediates for the synthesis of anticancer agents and neuroprotective drugs.
Patrizia Gentili - One of the best experts on this subject based on the ideXlab platform.
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Oxidative cleavage of 1-aryl-isochroman derivatives using the Trametes villosa laccase/1-Hydroxybenzotriazole system
New Journal of Chemistry, 2016Co-Authors: Roberta Bernini, Patrizia Gentili, Fernanda Crisante, Francesca D'acunzo, Emanuele UssiaAbstract:The oxidative cleavage of the dihydropyran ring of 1-aryl-isochroman derivatives was carried out for the first time under green chemistry conditions in the presence of the Trametes villosa laccase/1-Hydroxybenzotriazole system in buffered water/1,4-dioxane and buffered water/dimethyl carbonate as reaction media. The corresponding oxidation products [2-(2-hydroxyethyl)benzophenone derivatives] were obtained in different yields depending on the substituents on phenyl and isochroman rings. These compounds are useful intermediates for the synthesis of anticancer agents and neuroprotective drugs.
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oxidative cleavage of 1 aryl isochroman derivatives using the trametes villosa laccase 1 Hydroxybenzotriazole system
New Journal of Chemistry, 2016Co-Authors: Roberta Bernini, Patrizia Gentili, Fernanda Crisante, Francesca Dacunzo, Emanuele UssiaAbstract:The oxidative cleavage of the dihydropyran ring of 1-aryl-isochroman derivatives was carried out for the first time under green chemistry conditions in the presence of the Trametes villosa laccase/1-Hydroxybenzotriazole system in buffered water/1,4-dioxane and buffered water/dimethyl carbonate as reaction media. The corresponding oxidation products [2-(2-hydroxyethyl)benzophenone derivatives] were obtained in different yields depending on the substituents on phenyl and isochroman rings. These compounds are useful intermediates for the synthesis of anticancer agents and neuroprotective drugs.
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spectrophotometric epr and kinetic characterisation of the n o radical from 1 Hydroxybenzotriazole a key reactive species in mediated enzymatic oxidations
Chemical Communications, 2004Co-Authors: Carlo Galli, Patrizia Gentili, Osvaldo Lanzalunga, Marco Lucarini, Gian Franco PedulliAbstract:Characterisation of the aminoxyl (>N–O•) radical BTNO, generated from 1-Hydroxybenzotriazole (HBT) by the one-electron oxidant CAN (a CeIV salt), confirms BTNO as the reactive intermediate in oxidations run with the laccase/HBT system.
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Spectrophotometric, EPR and kinetic characterisation of the >N–O• radical from 1-Hydroxybenzotriazole, a key reactive species in mediated enzymatic oxidations
Chemical Communications, 2004Co-Authors: Carlo Galli, Patrizia Gentili, Osvaldo Lanzalunga, Marco Lucarini, Gian Franco PedulliAbstract:Characterisation of the aminoxyl (>N–O•) radical BTNO, generated from 1-Hydroxybenzotriazole (HBT) by the one-electron oxidant CAN (a CeIV salt), confirms BTNO as the reactive intermediate in oxidations run with the laccase/HBT system.
Hirofumi Hirai - One of the best experts on this subject based on the ideXlab platform.
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role of 1 Hydroxybenzotriazole in oxidation by laccase from trametes versicolor kinetic analysis of the laccase 1 Hydroxybenzotriazole couple
Fems Microbiology Letters, 2006Co-Authors: Hirofumi Hirai, Shingo Kawai, Hiroshi Shibata, Tomoaki NishidaAbstract:In the current studies, we used Lineweaver-Burke analysis to examine the role of 1-Hydroxybenzotriazole (HBT) in the oxidation of various compounds by laccase from Trametes versicolor. At low concentrations, HBT was a competitive inhibitor of the oxidation, but at high concentrations, it was a noncompetitive inhibitor. Analysis of the oxidation of ferrocytochrome c by the laccase-HBT couple showed that increasing the concentration of ferrocytochrome c did not affect the V(max) but reduced the apparent K(m). In addition, in the manganese peroxidase-Mn(II) reaction, which is a typical oxidation system by mediator, the apparent K(m) and V(max) increased as the concentration of the substrate 2,6-dimethoxyphenol was increased. These results indicate that HBT is involved in the binding of laccase and substrates that laccase cannot oxidize alone.
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Role of 1‐Hydroxybenzotriazole in oxidation by laccase from Trametes versicolor. Kinetic analysis of the laccase‐1‐Hydroxybenzotriazole couple
Fems Microbiology Letters, 2006Co-Authors: Hirofumi Hirai, Shingo Kawai, Hiroshi Shibata, Tomoaki NishidaAbstract:In the current studies, we used Lineweaver-Burke analysis to examine the role of 1-Hydroxybenzotriazole (HBT) in the oxidation of various compounds by laccase from Trametes versicolor. At low concentrations, HBT was a competitive inhibitor of the oxidation, but at high concentrations, it was a noncompetitive inhibitor. Analysis of the oxidation of ferrocytochrome c by the laccase-HBT couple showed that increasing the concentration of ferrocytochrome c did not affect the V(max) but reduced the apparent K(m). In addition, in the manganese peroxidase-Mn(II) reaction, which is a typical oxidation system by mediator, the apparent K(m) and V(max) increased as the concentration of the substrate 2,6-dimethoxyphenol was increased. These results indicate that HBT is involved in the binding of laccase and substrates that laccase cannot oxidize alone.
Roberta Bernini - One of the best experts on this subject based on the ideXlab platform.
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Oxidative cleavage of 1-aryl-isochroman derivatives using the Trametes villosa laccase/1-Hydroxybenzotriazole system
New Journal of Chemistry, 2016Co-Authors: Roberta Bernini, Patrizia Gentili, Fernanda Crisante, Francesca D'acunzo, Emanuele UssiaAbstract:The oxidative cleavage of the dihydropyran ring of 1-aryl-isochroman derivatives was carried out for the first time under green chemistry conditions in the presence of the Trametes villosa laccase/1-Hydroxybenzotriazole system in buffered water/1,4-dioxane and buffered water/dimethyl carbonate as reaction media. The corresponding oxidation products [2-(2-hydroxyethyl)benzophenone derivatives] were obtained in different yields depending on the substituents on phenyl and isochroman rings. These compounds are useful intermediates for the synthesis of anticancer agents and neuroprotective drugs.
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oxidative cleavage of 1 aryl isochroman derivatives using the trametes villosa laccase 1 Hydroxybenzotriazole system
New Journal of Chemistry, 2016Co-Authors: Roberta Bernini, Patrizia Gentili, Fernanda Crisante, Francesca Dacunzo, Emanuele UssiaAbstract:The oxidative cleavage of the dihydropyran ring of 1-aryl-isochroman derivatives was carried out for the first time under green chemistry conditions in the presence of the Trametes villosa laccase/1-Hydroxybenzotriazole system in buffered water/1,4-dioxane and buffered water/dimethyl carbonate as reaction media. The corresponding oxidation products [2-(2-hydroxyethyl)benzophenone derivatives] were obtained in different yields depending on the substituents on phenyl and isochroman rings. These compounds are useful intermediates for the synthesis of anticancer agents and neuroprotective drugs.
