Hymenium

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Rosa Mara Borges Da Silveira - One of the best experts on this subject based on the ideXlab platform.

Takashi Yajima - One of the best experts on this subject based on the ideXlab platform.

  • Pileus differentiation and pileus-specific protein expression in Flammulina velutipes.
    Fungal genetics and biology : FG & B, 2006
    Co-Authors: Yuichi Sakamoto, Akira Ando, Yutaka Tamai, Takashi Yajima
    Abstract:

    Fruiting bodies of Flammulina velutipes formed under complete darkness had a poorly developed pileus on top (pinhead fruiting body), and lacked a Hymenium. Upon light stimulation, the pileus immediately began to develop on the apical region of the pinhead fruiting body. Swelling of the apical region caused by cell division was observed 2 days after light treatment; at day 4, the junction fracture between the pileus and stipe, and formation of the Hymenium primordia were observed; at 6 days, gills were observed. We identified a cell wall-associated protein (PSH) that was specifically induced in the pileus, but not in the stipe, following light treatment of the pinhead fruiting body. Cloning and sequence analysis of the gene encoding PSH (psh) revealed a motif in the C-terminal region of the predicted amino acid sequence that was similar to hydrophobin. The level of transcription of psh was low in the stipe, but it was expressed at a high level in the pileus of the normal fruiting body. Transcription was also low in pinhead fruiting bodies, but increased after light treatment. These results indicate that psh is specifically expressed during pileus differentiation induced by light stimulation.

Vagner G. Cortez - One of the best experts on this subject based on the ideXlab platform.

Yuichi Sakamoto - One of the best experts on this subject based on the ideXlab platform.

  • Pileus differentiation and pileus-specific protein expression in Flammulina velutipes.
    Fungal genetics and biology : FG & B, 2006
    Co-Authors: Yuichi Sakamoto, Akira Ando, Yutaka Tamai, Takashi Yajima
    Abstract:

    Fruiting bodies of Flammulina velutipes formed under complete darkness had a poorly developed pileus on top (pinhead fruiting body), and lacked a Hymenium. Upon light stimulation, the pileus immediately began to develop on the apical region of the pinhead fruiting body. Swelling of the apical region caused by cell division was observed 2 days after light treatment; at day 4, the junction fracture between the pileus and stipe, and formation of the Hymenium primordia were observed; at 6 days, gills were observed. We identified a cell wall-associated protein (PSH) that was specifically induced in the pileus, but not in the stipe, following light treatment of the pinhead fruiting body. Cloning and sequence analysis of the gene encoding PSH (psh) revealed a motif in the C-terminal region of the predicted amino acid sequence that was similar to hydrophobin. The level of transcription of psh was low in the stipe, but it was expressed at a high level in the pileus of the normal fruiting body. Transcription was also low in pinhead fruiting bodies, but increased after light treatment. These results indicate that psh is specifically expressed during pileus differentiation induced by light stimulation.

Hibbett David - One of the best experts on this subject based on the ideXlab platform.