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Larry A. Hanson - One of the best experts on this subject based on the ideXlab platform.
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Complete Genome Sequence of an Ictalurid Herpesvirus 1 Strain Isolated from Blue Catfish (Ictalurus furcatus).
Microbiology Resource Announcements, 2019Co-Authors: Kuttichantran Subramaniam, Andrew J. Davison, Thomas B Waltzek, Arun Venugopalan, Matt J. Griffin, Lorelei Ford, Larry A. HansonAbstract:The complete genome sequence of an alloHerpesvirus isolated from blue catfish (Ictalurus furcatus) is reported. Genomic analyses revealed that this virus is a distinct strain of Ictalurid Herpesvirus 1, the first strain of which was isolated previously from a channel catfish (Ictalurus punctatus).
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AlloHerpesviruses of Fish
Aquaculture Virology, 2016Co-Authors: Larry A. Hanson, Andor Doszpoly, S.j. Van Beurden, P. H. De Oliveira Viadanna, Thomas B WaltzekAbstract:The family Alloherpesviridae includes Herpesviruses of fish and amphibians. This group of viruses are phylogenetically distant from the better characterized Herpesviruses found in birds and mammals. However, many biological and structural properties are conserved in the order Herpesvirales. The known alloHerpesviruses typically exhibit host specificity which is a biological feature shared by nearly all Herpesviruses. Of the alloHerpesviruses of fish at least 11 cause significant economic losses to aquaculture. These include: Ictalurid Herpesvirus 1 and 2 (IcHV1, 2) impacting catfish aquaculture; Cyprinid Herpesvirus 1, 2 and 3 (CyHV1-3) impacting carp and goldfish aquaculture; Anguillid Herpesvirus 1 (AngHV1) impacting eel aquaculture; Acipenserid Herpesvirus 1 and 2 (AciHV1, 2) impacting sturgeon aquaculture; and Salmonid Herpesvirus 2, 3 and 4 (SalHV2-4) impacting salmon and trout aquaculture. The most notable impact has been koi Herpesvirus disease caused by Cyprinid Herpesvirus 3. This virus has caused devastating losses worldwide in all strains of common carp and is an OIE notifiable pathogen.
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An over-lapping bacterial artificial chromosome system that generates vectorless progeny for channel catfish Herpesvirus
2015Co-Authors: Dusan Kunec, Larry A. Hanson, I. F. Nieuwenhuizen, Ra Van Haren, Shane C. BurgessAbstract:Herpesviruses are important pathogens of humans and other animals. Herpesvirus infectious clones that can reconstitute phenotypically wild-type (wt) virus are extremely valuable tools for elucidating the roles of specific genes in virus pathophysiology as well as for making vaccines. Ictalurid Herpesvirus 1 (channel catfish Herpesvirus [CCV]) is economically very important and is the best characterized of the Herpesviruses that occur primarily in bony fish and amphibians. Here, we describe the cloning of the hitherto recalcitrant CCV genome as three overlapping subgenomic bacterial artificial chromosomes (BACs). These clones allowed us to regenerate vectorless wt CCVs with a phenotype that is indistinguishable from that of the wt CCV from which the BACs were derived. To test the recombinogenic systems, we next used the overlapping BACs to construct a full-length CCV BAC by replacing the CCV ORF5 with the BAC cassette and cotransfecting CCO cells. The viral progeny that we used to transform Escherichia coli and the resulting BAC had only one of the 18-kb terminal repeated regions. Both systems suggest that one of the terminal repeat regions is lost during the replicative stage of the CCV life cycle. We also demonstrated the feasibility of introducing a targeted mutation into the CCV BAC infectious clone by constructing a CCV ORF12 deletion mutant and showed that ORF12 encodes a nonessential protein for virus replication. This is the first report of the generation of an infectious BAC clone of a member of the fish and amphibian Herpesviruses and its use to generate recombinants
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Catfish Virus (Ictalurid Herpesvirus 1) Temporal Gene Regulation of the Channel
2013Co-Authors: Suming Huang, Larry A. HansonAbstract:To identify promoter regions that impart differential temporal regulation of channel catfish virus (CCV)genes, the transcriptional kinetics of an immediate-early gene and prospective early and late genes were char-acterized. A cDNA clone, designated IE3C, representing a third immediate-early transcript was identified. The5
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Herpesviruses that infect fish.
Viruses, 2011Co-Authors: Larry A. Hanson, Arnon Dishon, Moshe KotlerAbstract:Herpesviruses are host specific pathogens that are widespread among vertebrates. Genome sequence data demonstrate that most Herpesviruses of fish and amphibians are grouped together (family Alloherpesviridae) and are distantly related to Herpesviruses of reptiles, birds and mammals (family Herpesviridae). Yet, many of the biological processes of members of the order Herpesvirales are similar. Among the conserved characteristics are the virion structure, replication process, the ability to establish long term latency and the manipulation of the host immune response. Many of the similar processes may be due to convergent evolution. This overview of identified Herpesviruses of fish discusses the diseases that alloHerpesviruses cause, the biology of these viruses and the host-pathogen interactions. Much of our knowledge on the biology of Alloherpesvirdae is derived from research with two species: Ictalurid Herpesvirus 1 (channel catfish virus) and Cyprinid Herpesvirus 3 (koi Herpesvirus).
Andrew J. Davison - One of the best experts on this subject based on the ideXlab platform.
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Complete Genome Sequence of an Ictalurid Herpesvirus 1 Strain Isolated from Blue Catfish (Ictalurus furcatus).
Microbiology Resource Announcements, 2019Co-Authors: Kuttichantran Subramaniam, Andrew J. Davison, Thomas B Waltzek, Arun Venugopalan, Matt J. Griffin, Lorelei Ford, Larry A. HansonAbstract:The complete genome sequence of an alloHerpesvirus isolated from blue catfish (Ictalurus furcatus) is reported. Genomic analyses revealed that this virus is a distinct strain of Ictalurid Herpesvirus 1, the first strain of which was isolated previously from a channel catfish (Ictalurus punctatus).
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Genome sequences of three koi Herpesvirus isolates representing the expanding distribution of an emerging disease threatening koi and common carp worldwide
Journal of Virology, 2007Co-Authors: Takashi Aoki, Ikuo Hirono, Andrew J. Davison, Hideo Fukuda, Thomas B Waltzek, Ken Kurokawa, Ronen Nahary, Avi Eldar, Herve Bercovier, Ronald P HedrickAbstract:Since the mid-1990s, lethal infections of koi Herpesvirus (KHV) have been spreading, threatening the worldwide production of common carp and koi (both Cyprinus carpio). The complete genome sequences of three KHV strains from Japan, the United States, and Israel revealed a 295-kbp genome containing a 22-kbp terminal direct repeat. The finding that 15 KHV genes have clear homologs in the distantly related channel catfish virus (Ictalurid Herpesvirus 1) confirms the proposed place of KHV in the family Herpesviridae, specifically in the branch with fish and amphibian hosts. KHV thus has the largest genome reported to date for this family. The three strains were interpreted as having arisen from a wild-type parent encoding 156 unique protein-coding genes, 8 of which are duplicated in the terminal repeat. In each strain, four to seven genes from among a set of nine are fragmented by frameshifts likely to render the encoded proteins nonfunctional. Six of the affected genes encode predicted membrane glycoproteins. Frameshifts or other mutations close to the 3' ends of coding sequences were identified in a further six genes. The conclusion that at least some of these mutations occurred in vivo prompts the hypothesis that loss of gene functions might be associated with emergence of the disease and provides a basis for further investigations into the molecular epidemiology of the virus.
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Genome sequences of two frog Herpesviruses
Journal of General Virology, 2006Co-Authors: Andrew J. Davison, Charles Cunningham, Walter Sauerbier, Robert G. MckinnellAbstract:The sequences of two frog Herpesviruses, Ranid Herpesvirus 1 and Ranid Herpesvirus 2, were determined. They are respectively 220 859 and 231 801 bp in size and contain 132 and 147 predicted genes. The genomes are related most closely in the central regions, where 40 genes are conserved convincingly. Nineteen of these genes are also conserved in a fish Herpesvirus, Ictalurid Herpesvirus 1. The terminal regions of the genomes are largely not conserved and contain many of the 15 families of related genes present in each genome. The frog Herpesviruses are unique among sequenced Herpesviruses in that the three exons of the gene encoding the putative ATPase subunit of terminase are not specified by the same DNA strand and in that they encode a putative DNA (cytosine-5-)-methyltransferase and have extensively methylated genomes.
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Correspondence
2006Co-Authors: Walter Sauerbier, Charles Cunningham, Robert G. Mckinnell, Andrew J. DavisonAbstract:The sequences of two frog Herpesviruses, Ranid Herpesvirus 1 and Ranid Herpesvirus 2, were determined. They are respectively 220859 and 231801 bp in size and contain 132 and 147 predicted genes. The genomes are related most closely in the central regions, where 40 genes are conserved convincingly. Nineteen of these genes are also conserved in a fish Herpesvirus, Ictalurid Herpesvirus 1. The terminal regions of the genomes are largely not conserved and contain many of the 15 families of related genes present in each genome. The frog Herpesviruses are unique among sequenced Herpesviruses in that the three exons of the gene encoding the putative ATPase subunit of terminase are not specified by the same DNA strand and in that they encode a putative DNA (cytosine-5-)-methyltransferase and have extensively methylated genomes. Herpesviruses are defined morphologically by a T=16 icosahedral capsid and fall phylogenetically into three tenuously related groups corresponding to host class: those infecting Mammalia, Aves and Reptilia, those infecting Amphibia and Osteichthyes (bony fish) and a single virus that infects an invertebrate class, Bivalvia (Davison et al., 2005a; McGeoch et al., 2006). Two amphibian Herpesviruses have been classified: Ranid Herpesvirus 1 (RaHV-1; Lucke ́ tumour Herpesvirus) and Ranid Herpesvirus 2 (RaHV-2; frog virus 4). Analysis of 40 kbp of the genome has shown that RaHV-1 is related to Ictalurid Herpesvirus 1 (IcHV-1; channel catfish virus) (Davison, 1992; Davison et al., 1999). No sequence data are available for RaHV-2. The properties of RaHV-1 and RaHV-2 have been reviewed by Granoff (1999). RaHV-1 is the causative agent of a renal adenocarcinoma occurring in the leopard frog, Rana pipien
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Koi Herpesvirus represents a third cyprinid Herpesvirus (CyHV-3) in the family Herpesviridae
Journal of General Virology, 2005Co-Authors: Thomas B Waltzek, Larry A. Hanson, Garry O. Kelley, Ikuo Hirono, Andrew J. Davison, Hideo Fukuda, David M Stone, Takashi Aoki, Ronald P HedrickAbstract:The sequences of four complete genes were analysed in order to determine the relatedness of koi Herpesvirus (KHV) to three fish viruses in the family Herpesviridae: carp pox Herpesvirus (Cyprinid Herpesvirus 1, CyHV-1), haematopoietic necrosis Herpesvirus of goldfish (Cyprinid Herpesvirus 2, CyHV-2) and channel catfish virus (Ictalurid Herpesvirus 1, IcHV-1). The genes were predicted to encode a helicase, an intercapsomeric triplex protein, the DNA polymerase and the major capsid protein. The results showed that KHV is related closely to CyHV-1 and CyHV-2, and that the three cyprinid viruses are related, albeit more distantly, to IcHV-1. Twelve KHV isolates from four diverse geographical areas yielded identical sequences for a region of the DNA polymerase gene. These findings, with previously published morphological and biological data, indicate that KHV should join the group of related lower-vertebrate viruses in the family Herpesviridae under the formal designation Cyprinid Herpesvirus 3 (CyHV-3).
Robert G. Mckinnell - One of the best experts on this subject based on the ideXlab platform.
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Genome sequences of two frog Herpesviruses
Journal of General Virology, 2006Co-Authors: Andrew J. Davison, Charles Cunningham, Walter Sauerbier, Robert G. MckinnellAbstract:The sequences of two frog Herpesviruses, Ranid Herpesvirus 1 and Ranid Herpesvirus 2, were determined. They are respectively 220 859 and 231 801 bp in size and contain 132 and 147 predicted genes. The genomes are related most closely in the central regions, where 40 genes are conserved convincingly. Nineteen of these genes are also conserved in a fish Herpesvirus, Ictalurid Herpesvirus 1. The terminal regions of the genomes are largely not conserved and contain many of the 15 families of related genes present in each genome. The frog Herpesviruses are unique among sequenced Herpesviruses in that the three exons of the gene encoding the putative ATPase subunit of terminase are not specified by the same DNA strand and in that they encode a putative DNA (cytosine-5-)-methyltransferase and have extensively methylated genomes.
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Correspondence
2006Co-Authors: Walter Sauerbier, Charles Cunningham, Robert G. Mckinnell, Andrew J. DavisonAbstract:The sequences of two frog Herpesviruses, Ranid Herpesvirus 1 and Ranid Herpesvirus 2, were determined. They are respectively 220859 and 231801 bp in size and contain 132 and 147 predicted genes. The genomes are related most closely in the central regions, where 40 genes are conserved convincingly. Nineteen of these genes are also conserved in a fish Herpesvirus, Ictalurid Herpesvirus 1. The terminal regions of the genomes are largely not conserved and contain many of the 15 families of related genes present in each genome. The frog Herpesviruses are unique among sequenced Herpesviruses in that the three exons of the gene encoding the putative ATPase subunit of terminase are not specified by the same DNA strand and in that they encode a putative DNA (cytosine-5-)-methyltransferase and have extensively methylated genomes. Herpesviruses are defined morphologically by a T=16 icosahedral capsid and fall phylogenetically into three tenuously related groups corresponding to host class: those infecting Mammalia, Aves and Reptilia, those infecting Amphibia and Osteichthyes (bony fish) and a single virus that infects an invertebrate class, Bivalvia (Davison et al., 2005a; McGeoch et al., 2006). Two amphibian Herpesviruses have been classified: Ranid Herpesvirus 1 (RaHV-1; Lucke ́ tumour Herpesvirus) and Ranid Herpesvirus 2 (RaHV-2; frog virus 4). Analysis of 40 kbp of the genome has shown that RaHV-1 is related to Ictalurid Herpesvirus 1 (IcHV-1; channel catfish virus) (Davison, 1992; Davison et al., 1999). No sequence data are available for RaHV-2. The properties of RaHV-1 and RaHV-2 have been reviewed by Granoff (1999). RaHV-1 is the causative agent of a renal adenocarcinoma occurring in the leopard frog, Rana pipien
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Genomic studies of the Lucké tumor Herpesvirus (RaHV-1)
Journal of Cancer Research and Clinical Oncology, 1999Co-Authors: Andrew J. Davison, Walter Sauerbier, Aidan Dolan, Clare Addison, Robert G. MckinnellAbstract:Ranid Herpesvirus 1 (RaHV-1) is the etiological agent of the Lucké renal adenocarcinoma of the North American leopard frog Rana pipiens. Construction of cosmid libraries containing RaHV-1 DNA inserts allowed the derivation of a Bam HI map for the viral genome. Summation of fragment sizes indicates that the genome is 217 kbp in size, a value in accordance with the most recent published estimate (220 kbp) obtained by field-inversion gel electrophoresis. The DNA sequence of the 39,757-bp insert in 1 cosmid (cos54) was determined and was predicted to contain 21 complete and 3 partial genes. In all, 12 genes have distant counterparts in a fish Herpesvirus (Ictalurid Herpesvirus 1) and are present in 2 blocks, 1 of which is relatively inverted. This indicates that RaHV-1 belongs to the fish virus lineage of the Herpesvirus family rather than to the lineage populated by mammalian and avian viruses. The remainding 12 genes in cos54 lack counterparts in any other Herpesvirus. One of these encodes a putative DNA (cytosine-5) methyltransferase. This raises the possibility that biological processes induced in the host by RaHV-1 might involve methylation of cellular DNA by the viral enzyme.
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Genomic studies of the Lucké tumor Herpesvirus (RaHV-1)
Journal of Cancer Research and Clinical Oncology, 1999Co-Authors: Andrew J. Davison, Walter Sauerbier, Aidan Dolan, Clare Addison, Robert G. MckinnellAbstract:Ranid Herpesvirus 1 (RaHV-1) is the etiological agent of the Lucke renal adenocarcinoma of the North American leopard frog Rana pipiens. Construction of cosmid libraries containing RaHV-1 DNA inserts allowed the derivation of a BamHI map for the viral genome. Summation of fragment sizes indicates that the genome is 217 kbp in size, a value in accordance with the most recent published estimate (220 kbp) obtained by field-inversion gel electrophoresis. The DNA sequence of the 39,757-bp insert in 1 cosmid (cos54) was determined and was predicted to contain 21 complete and 3 partial genes. In all, 12 genes have distant counterparts in a fish Herpesvirus (Ictalurid Herpesvirus 1) and are present in 2 blocks, 1 of which is relatively inverted. This indicates that RaHV-1 belongs to the fish virus lineage of the Herpesvirus family rather than to the lineage populated by mammalian and avian viruses. The remainding 12 genes in cos54 lack counterparts in any other Herpesvirus. One of these encodes a putative DNA (cytosine-5) methyltransferase. This raises the possibility that biological processes induced in the host by RaHV-1 might involve methylation of cellular DNA by the viral enzyme.
Ronald P Hedrick - One of the best experts on this subject based on the ideXlab platform.
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Genome sequences of three koi Herpesvirus isolates representing the expanding distribution of an emerging disease threatening koi and common carp worldwide
Journal of Virology, 2007Co-Authors: Takashi Aoki, Ikuo Hirono, Andrew J. Davison, Hideo Fukuda, Thomas B Waltzek, Ken Kurokawa, Ronen Nahary, Avi Eldar, Herve Bercovier, Ronald P HedrickAbstract:Since the mid-1990s, lethal infections of koi Herpesvirus (KHV) have been spreading, threatening the worldwide production of common carp and koi (both Cyprinus carpio). The complete genome sequences of three KHV strains from Japan, the United States, and Israel revealed a 295-kbp genome containing a 22-kbp terminal direct repeat. The finding that 15 KHV genes have clear homologs in the distantly related channel catfish virus (Ictalurid Herpesvirus 1) confirms the proposed place of KHV in the family Herpesviridae, specifically in the branch with fish and amphibian hosts. KHV thus has the largest genome reported to date for this family. The three strains were interpreted as having arisen from a wild-type parent encoding 156 unique protein-coding genes, 8 of which are duplicated in the terminal repeat. In each strain, four to seven genes from among a set of nine are fragmented by frameshifts likely to render the encoded proteins nonfunctional. Six of the affected genes encode predicted membrane glycoproteins. Frameshifts or other mutations close to the 3' ends of coding sequences were identified in a further six genes. The conclusion that at least some of these mutations occurred in vivo prompts the hypothesis that loss of gene functions might be associated with emergence of the disease and provides a basis for further investigations into the molecular epidemiology of the virus.
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Koi Herpesvirus represents a third cyprinid Herpesvirus (CyHV-3) in the family Herpesviridae
Journal of General Virology, 2005Co-Authors: Thomas B Waltzek, Larry A. Hanson, Garry O. Kelley, Ikuo Hirono, Andrew J. Davison, Hideo Fukuda, David M Stone, Takashi Aoki, Ronald P HedrickAbstract:The sequences of four complete genes were analysed in order to determine the relatedness of koi Herpesvirus (KHV) to three fish viruses in the family Herpesviridae: carp pox Herpesvirus (Cyprinid Herpesvirus 1, CyHV-1), haematopoietic necrosis Herpesvirus of goldfish (Cyprinid Herpesvirus 2, CyHV-2) and channel catfish virus (Ictalurid Herpesvirus 1, IcHV-1). The genes were predicted to encode a helicase, an intercapsomeric triplex protein, the DNA polymerase and the major capsid protein. The results showed that KHV is related closely to CyHV-1 and CyHV-2, and that the three cyprinid viruses are related, albeit more distantly, to IcHV-1. Twelve KHV isolates from four diverse geographical areas yielded identical sequences for a region of the DNA polymerase gene. These findings, with previously published morphological and biological data, indicate that KHV should join the group of related lower-vertebrate viruses in the family Herpesviridae under the formal designation Cyprinid Herpesvirus 3 (CyHV-3).
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Short Communication Koi Herpesvirus represents a third cyprinid Herpesvirus (CyHV-3) in the family Herpesviridae
2005Co-Authors: Thomas B Waltzek, Larry A. Hanson, Garry O. Kelley, Ikuo Hirono, Andrew J. Davison, Hideo Fukuda, David M Stone, Takashi Aoki, Ronald P HedrickAbstract:The sequences of four complete genes were analysed in order to determine the relatedness of koi Herpesvirus (KHV) to three fish viruses in the family Herpesviridae: carp pox Herpesvirus (Cyprinid Herpesvirus 1, CyHV-1), haematopoietic necrosis Herpesvirus of goldfish (Cyprinid Herpesvirus 2, CyHV-2) and channel catfish virus (Ictalurid Herpesvirus 1, IcHV-1). The genes were predicted to encode a helicase, an intercapsomeric triplex protein, the DNA polymerase and the major capsid protein. The results showed that KHV is related closely to CyHV-1 and CyHV-2, and that the three cyprinid viruses are related, albeit more distantly, to IcHV-1. Twelve KHV isolates from four diverse geographical areas yielded identical sequences for a region of the DNA polymerase gene. These findings, with previously published morphological and biological data, indicate that KHV should join the group of related lower-vertebrate viruses in the family Herpesviridae under the formal designation Cyprinid Herpesvirus 3 (CyHV-3).
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Concentrations of a Koi Herpesvirus (KHV) in tissues of experimentally infected Cyprinus carpio koi as assessed by real-time TaqMan PCR.
Diseases of Aquatic Organisms, 2004Co-Authors: Oren Gilad, Herve Bercovier, Susan Yun, Francisco J. Zagmutt-vergara, Christian M. Leutenegger, Ronald P HedrickAbstract:The Koi Herpesvirus (KHV) is a herpes-like virus now recognized as a worldwide cause of mortality among populations of koi Cyprinus carpio koi and common carp Cyprinus carpio carpio. Temperature is a key factor influencing virus replication both in cell culture and in the tissues of experimentally infected fish. Genomic DNA sequences were used to optimize a rapid real-time Taq- Man PCR assay to detect and quantify KHV DNA as found in the tissues of virus-exposed fish. The assay allowed analytical enumeration of target KHV genome copies ranging from 10 1 to 10 7 mole- cules as present in infected cell lines or fish tissues. The new assay was specific for KHV and did not detect DNA from 3 related herpes-like viruses found in fish, the Cyprinid Herpesvirus 1 (CyHV-1), Cyprinid Herpesvirus 2 (CyHV-2), Ictalurid Herpesvirus 1 (IcHV-1) or the KF-1 cell line used for virus growth. Concentrations of KHV DNA were evaluated in 7 different tissues of replicate groups of virus-exposed koi held at water temperatures of 13, 18, 23 and 28°C. Viral DNA was detected among virus-exposed koi at all 4 water temperatures but mortality was only observed among fish at 18, 23, and 28°C. Time and temperature and the interactions between them affected concentrations of viral DNA detected in tissues of koi exposed to KHV. Although there were no recognized patterns to viral DNA concentrations as found in different tissues over time, KHV genome copies for all tissues increased with time post virus exposure and with water temperature. The remarkably rapid and sys- temic spread of the virus was demonstrated by the presence of viral DNA in multiple tissues 1 d post virus exposure. The greatest DNA concentrations found were in the gill, kidney and spleen, with virus genome equivalents consistently from 10 8 to 10 9 per 10 6 host cells. High levels of KHV DNA were also found in the mucus, liver, gut, and brain. Koi surviving infection at 62 to 64 d post virus exposure contained lower KHV genome copies (up to 1.99 × 10 2 per 10 6 host cells) as present in gill,
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Systemic herpes-like virus in catfish Ictalurus melas (Italy) differs from Ictalurid Herpesvirus 1 (North America).
Diseases of Aquatic Organisms, 2003Co-Authors: Ronald P Hedrick, Terry S. Mcdowell, Oren Gilad, Mark A. Adkison, Giuseppe BovoAbstract:A Herpesvirus was isolated during 2 occurrences of mass mortality among adult catfish Ictalurus melas raised in different farms in northern Italy. The agent replicated in the channel catfish ovary (CCO) cell line from channel catfish I. punctatus, inducing a cytopathic effect similar to that caused by Ictalurid Herpesvirus 1 (also referred to as channel catfish Herpesvirus, CCV). The new Herpesvirus, designated I. melas Herpesvirus (IcmHV) did not react with polyclonal rabbit or mono- clonal antibodies directed to CCV in either neutralization or indirect immunofluorescence assays. The virions of IcmHV possessed a hexagonal nucleocapsid of 107 nm in diameter surrounded by an envelope with a diameter of 227 nm (n = 20) typical for members of the family Herpesviridae. Virions of IcmHV purified from infected CCO cells contained 17 polypeptides ranging in size from 17.5 to 175 kDa and most differed in molecular weight from those found for CCV. The IcmHV was also dis- tinct from CCV when compared by restriction fragment length polymorphisms (RFLP) of genomic DNA following digestions with the endonucleases Kpn I and Sac I. Lastly, the virulence of IcmHV for channel catfish fry and juveniles, respectively, was demonstrated by experimental infections induced by bath exposure or intraperitoneal injection that resulted in 78 to 96% cumulative mortality in groups of exposed fish. Preventing the introduction of this agent into geographic regions where significant channel catfish production occurs should be a high priority.
Walter Sauerbier - One of the best experts on this subject based on the ideXlab platform.
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Genome sequences of two frog Herpesviruses
Journal of General Virology, 2006Co-Authors: Andrew J. Davison, Charles Cunningham, Walter Sauerbier, Robert G. MckinnellAbstract:The sequences of two frog Herpesviruses, Ranid Herpesvirus 1 and Ranid Herpesvirus 2, were determined. They are respectively 220 859 and 231 801 bp in size and contain 132 and 147 predicted genes. The genomes are related most closely in the central regions, where 40 genes are conserved convincingly. Nineteen of these genes are also conserved in a fish Herpesvirus, Ictalurid Herpesvirus 1. The terminal regions of the genomes are largely not conserved and contain many of the 15 families of related genes present in each genome. The frog Herpesviruses are unique among sequenced Herpesviruses in that the three exons of the gene encoding the putative ATPase subunit of terminase are not specified by the same DNA strand and in that they encode a putative DNA (cytosine-5-)-methyltransferase and have extensively methylated genomes.
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Correspondence
2006Co-Authors: Walter Sauerbier, Charles Cunningham, Robert G. Mckinnell, Andrew J. DavisonAbstract:The sequences of two frog Herpesviruses, Ranid Herpesvirus 1 and Ranid Herpesvirus 2, were determined. They are respectively 220859 and 231801 bp in size and contain 132 and 147 predicted genes. The genomes are related most closely in the central regions, where 40 genes are conserved convincingly. Nineteen of these genes are also conserved in a fish Herpesvirus, Ictalurid Herpesvirus 1. The terminal regions of the genomes are largely not conserved and contain many of the 15 families of related genes present in each genome. The frog Herpesviruses are unique among sequenced Herpesviruses in that the three exons of the gene encoding the putative ATPase subunit of terminase are not specified by the same DNA strand and in that they encode a putative DNA (cytosine-5-)-methyltransferase and have extensively methylated genomes. Herpesviruses are defined morphologically by a T=16 icosahedral capsid and fall phylogenetically into three tenuously related groups corresponding to host class: those infecting Mammalia, Aves and Reptilia, those infecting Amphibia and Osteichthyes (bony fish) and a single virus that infects an invertebrate class, Bivalvia (Davison et al., 2005a; McGeoch et al., 2006). Two amphibian Herpesviruses have been classified: Ranid Herpesvirus 1 (RaHV-1; Lucke ́ tumour Herpesvirus) and Ranid Herpesvirus 2 (RaHV-2; frog virus 4). Analysis of 40 kbp of the genome has shown that RaHV-1 is related to Ictalurid Herpesvirus 1 (IcHV-1; channel catfish virus) (Davison, 1992; Davison et al., 1999). No sequence data are available for RaHV-2. The properties of RaHV-1 and RaHV-2 have been reviewed by Granoff (1999). RaHV-1 is the causative agent of a renal adenocarcinoma occurring in the leopard frog, Rana pipien
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Genomic studies of the Lucké tumor Herpesvirus (RaHV-1)
Journal of Cancer Research and Clinical Oncology, 1999Co-Authors: Andrew J. Davison, Walter Sauerbier, Aidan Dolan, Clare Addison, Robert G. MckinnellAbstract:Ranid Herpesvirus 1 (RaHV-1) is the etiological agent of the Lucké renal adenocarcinoma of the North American leopard frog Rana pipiens. Construction of cosmid libraries containing RaHV-1 DNA inserts allowed the derivation of a Bam HI map for the viral genome. Summation of fragment sizes indicates that the genome is 217 kbp in size, a value in accordance with the most recent published estimate (220 kbp) obtained by field-inversion gel electrophoresis. The DNA sequence of the 39,757-bp insert in 1 cosmid (cos54) was determined and was predicted to contain 21 complete and 3 partial genes. In all, 12 genes have distant counterparts in a fish Herpesvirus (Ictalurid Herpesvirus 1) and are present in 2 blocks, 1 of which is relatively inverted. This indicates that RaHV-1 belongs to the fish virus lineage of the Herpesvirus family rather than to the lineage populated by mammalian and avian viruses. The remainding 12 genes in cos54 lack counterparts in any other Herpesvirus. One of these encodes a putative DNA (cytosine-5) methyltransferase. This raises the possibility that biological processes induced in the host by RaHV-1 might involve methylation of cellular DNA by the viral enzyme.
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Genomic studies of the Lucké tumor Herpesvirus (RaHV-1)
Journal of Cancer Research and Clinical Oncology, 1999Co-Authors: Andrew J. Davison, Walter Sauerbier, Aidan Dolan, Clare Addison, Robert G. MckinnellAbstract:Ranid Herpesvirus 1 (RaHV-1) is the etiological agent of the Lucke renal adenocarcinoma of the North American leopard frog Rana pipiens. Construction of cosmid libraries containing RaHV-1 DNA inserts allowed the derivation of a BamHI map for the viral genome. Summation of fragment sizes indicates that the genome is 217 kbp in size, a value in accordance with the most recent published estimate (220 kbp) obtained by field-inversion gel electrophoresis. The DNA sequence of the 39,757-bp insert in 1 cosmid (cos54) was determined and was predicted to contain 21 complete and 3 partial genes. In all, 12 genes have distant counterparts in a fish Herpesvirus (Ictalurid Herpesvirus 1) and are present in 2 blocks, 1 of which is relatively inverted. This indicates that RaHV-1 belongs to the fish virus lineage of the Herpesvirus family rather than to the lineage populated by mammalian and avian viruses. The remainding 12 genes in cos54 lack counterparts in any other Herpesvirus. One of these encodes a putative DNA (cytosine-5) methyltransferase. This raises the possibility that biological processes induced in the host by RaHV-1 might involve methylation of cellular DNA by the viral enzyme.
