The Experts below are selected from a list of 9381 Experts worldwide ranked by ideXlab platform
Shoichiro Kurata - One of the best experts on this subject based on the ideXlab platform.
-
Nuclear Lamin is required for Winged Eye-mediated transdetermination of Drosophila Imaginal Disc.
Genes to Cells, 2018Co-Authors: Keita Masuko, Naoyuki Fuse, Kanae Komaba, Rumi Nakajima, Hirofumi Furuhashi, Eriko Numao, Shoichiro KurataAbstract:Drosophila Imaginal Discs often change their cell fate under stress conditions, and this phenomenon, called transdetermination (TD), has long been a useful model for studying cell fate plasticity during regeneration. We previously identified a chromatin-associated protein, Winged Eye (Wge), which induces eye-to-wing TD upon its over-expression in eye Imaginal Discs. However, the molecular mechanism of Wge-mediated TD remains obscure. Here, we analyzed Wge-interacting proteins and found that several heterochromatin-related proteins, including a nuclear lamina protein, Lamin (Lam), were associated with Wge protein in cultured cells. Knockdown experiments revealed that Lam is indeed required for Wge-mediated eye-to-wing TD. Moreover, Wge over-expression altered the spatial organization of genomic DNA inside the cell nuclei. Accordingly, we suggest that Wge interacts with Lam to link some genomic regions with the nuclear periphery and regulates chromatin dynamics in Imaginal Disc TD.
-
winged eye induces transdetermination of drosophila Imaginal Disc by acting in concert with a histone methyltransferase su var 3 9
Cell Reports, 2018Co-Authors: Keita Masuko, Tomonori Katsuyama, Naoyuki Fuse, Kanae Komaba, Rumi Nakajima, Hirofumi Furuhashi, Shoichiro KurataAbstract:Drosophila Imaginal Disc cells exhibit a remarkable ability to convert cell fates in response to various perturbations, a phenomenon called transdetermination (TD). We previously identified winged eye (wge) as a factor that induces eye-to-wing TD upon overexpression in eye Imaginal Discs, but the molecular mechanisms underlying TD have remained largely unclear. Here, we found that wge induces various histone modifications and enhances the methylation of Lys9 on histone H3 (H3K9), a feature of heterochromatin. A histone methyltransferase, Su(var)3-9, is required for wge-mediated H3K9 methylation and eye-to-wing TD. Su(var)3-9 is also required for classical wound-induced TD but not for normal development, suggesting its involvement in several types of Imaginal Disc TDs. Transcriptome analysis revealed that wge represses eye identity genes independently of Su(var)3-9 and activates TD-related genes by acting together with Su(var)3-9. These findings provide new insights into diverse types of chromatin regulation at progressive steps of cell-fate conversions.
-
winged eye Induces Transdetermination of Drosophila Imaginal Disc by Acting in Concert with a Histone Methyltransferase, Su(var)3-9
Elsevier, 2018Co-Authors: Keita Masuko, Tomonori Katsuyama, Naoyuki Fuse, Kanae Komaba, Rumi Nakajima, Hirofumi Furuhashi, Shoichiro KurataAbstract:Summary: Drosophila Imaginal Disc cells exhibit a remarkable ability to convert cell fates in response to various perturbations, a phenomenon called transdetermination (TD). We previously identified winged eye (wge) as a factor that induces eye-to-wing TD upon overexpression in eye Imaginal Discs, but the molecular mechanisms underlying TD have remained largely unclear. Here, we found that wge induces various histone modifications and enhances the methylation of Lys9 on histone H3 (H3K9), a feature of heterochromatin. A histone methyltransferase, Su(var)3-9, is required for wge-mediated H3K9 methylation and eye-to-wing TD. Su(var)3-9 is also required for classical wound-induced TD but not for normal development, suggesting its involvement in several types of Imaginal Disc TDs. Transcriptome analysis revealed that wge represses eye identity genes independently of Su(var)3-9 and activates TD-related genes by acting together with Su(var)3-9. These findings provide new insights into diverse types of chromatin regulation at progressive steps of cell-fate conversions. : Drosophila Imaginal Discs switch Disc identity by a process known as transdetermination. Masuko et al. demonstrate that expression of the winged eye gene induces transdetermination through histone modifications such as H3K9-methylation. winged eye regulates expression of transdetermination-related genes via a histone methyltransferase, Su(var)3-9. Keywords: Drosophila, Imaginal Disc, transdetermination, heterochromatin, cell fate, winged eye, reprogramming, Su(var)3-
Tomonori Katsuyama - One of the best experts on this subject based on the ideXlab platform.
-
winged eye induces transdetermination of drosophila Imaginal Disc by acting in concert with a histone methyltransferase su var 3 9
Cell Reports, 2018Co-Authors: Keita Masuko, Tomonori Katsuyama, Naoyuki Fuse, Kanae Komaba, Rumi Nakajima, Hirofumi Furuhashi, Shoichiro KurataAbstract:Drosophila Imaginal Disc cells exhibit a remarkable ability to convert cell fates in response to various perturbations, a phenomenon called transdetermination (TD). We previously identified winged eye (wge) as a factor that induces eye-to-wing TD upon overexpression in eye Imaginal Discs, but the molecular mechanisms underlying TD have remained largely unclear. Here, we found that wge induces various histone modifications and enhances the methylation of Lys9 on histone H3 (H3K9), a feature of heterochromatin. A histone methyltransferase, Su(var)3-9, is required for wge-mediated H3K9 methylation and eye-to-wing TD. Su(var)3-9 is also required for classical wound-induced TD but not for normal development, suggesting its involvement in several types of Imaginal Disc TDs. Transcriptome analysis revealed that wge represses eye identity genes independently of Su(var)3-9 and activates TD-related genes by acting together with Su(var)3-9. These findings provide new insights into diverse types of chromatin regulation at progressive steps of cell-fate conversions.
-
winged eye Induces Transdetermination of Drosophila Imaginal Disc by Acting in Concert with a Histone Methyltransferase, Su(var)3-9
Elsevier, 2018Co-Authors: Keita Masuko, Tomonori Katsuyama, Naoyuki Fuse, Kanae Komaba, Rumi Nakajima, Hirofumi Furuhashi, Shoichiro KurataAbstract:Summary: Drosophila Imaginal Disc cells exhibit a remarkable ability to convert cell fates in response to various perturbations, a phenomenon called transdetermination (TD). We previously identified winged eye (wge) as a factor that induces eye-to-wing TD upon overexpression in eye Imaginal Discs, but the molecular mechanisms underlying TD have remained largely unclear. Here, we found that wge induces various histone modifications and enhances the methylation of Lys9 on histone H3 (H3K9), a feature of heterochromatin. A histone methyltransferase, Su(var)3-9, is required for wge-mediated H3K9 methylation and eye-to-wing TD. Su(var)3-9 is also required for classical wound-induced TD but not for normal development, suggesting its involvement in several types of Imaginal Disc TDs. Transcriptome analysis revealed that wge represses eye identity genes independently of Su(var)3-9 and activates TD-related genes by acting together with Su(var)3-9. These findings provide new insights into diverse types of chromatin regulation at progressive steps of cell-fate conversions. : Drosophila Imaginal Discs switch Disc identity by a process known as transdetermination. Masuko et al. demonstrate that expression of the winged eye gene induces transdetermination through histone modifications such as H3K9-methylation. winged eye regulates expression of transdetermination-related genes via a histone methyltransferase, Su(var)3-9. Keywords: Drosophila, Imaginal Disc, transdetermination, heterochromatin, cell fate, winged eye, reprogramming, Su(var)3-
-
Imaginal Disc Transplantation in Drosophila
Methods in Molecular Biology, 2016Co-Authors: Tomonori Katsuyama, Renato ParoAbstract:Since Ephrussi and Beadle introduced Imaginal Disc transplantation to Drosophila research in 1936, the method played an important part towards a better understanding of Disc patterning, tissue regeneration, and reprogramming phenomena like transdetermination. Despite increasing usage of high-throughput approaches towards solving biological problems this classical manual method is still in use for studying Disc development in a semi-physiological context. Here we describe in detail a protocol and provide recommendations on the procedure in particular for analyzing the regenerative potential of Imaginal disks. The steps consist of Disc dissection and fragmentation, transplantation into the larval or adult abdomen, and the recovery of implants from the host abdomen. Additionally, we also describe how to make the special transplantation needle from a glass capillary.
-
tissue nonautonomous effects of fat body methionine metabolism on Imaginal Disc repair in drosophila
Proceedings of the National Academy of Sciences of the United States of America, 2016Co-Authors: Soshiro Kashio, Tomonori Katsuyama, Fumiaki Obata, Liu Zhang, Takahiro Chihara, Masayuki MiuraAbstract:Regulatory mechanisms for tissue repair and regeneration within damaged tissue have been extensively studied. However, the systemic regulation of tissue repair remains poorly understood. To elucidate tissue nonautonomous control of repair process, it is essential to induce local damage, independent of genetic manipulations in uninjured parts of the body. Herein, we develop a system in Drosophila for spatiotemporal tissue injury using a temperature-sensitive form of diphtheria toxin A domain driven by the Q system to study factors contributing to Imaginal Disc repair. Using this technique, we demonstrate that methionine metabolism in the fat body, a counterpart of mammalian liver and adipose tissue, supports the repair processes of wing Discs. Local injury to wing Discs decreases methionine and S-adenosylmethionine, whereas it increases S-adenosylhomocysteine in the fat body. Fat body-specific genetic manipulation of methionine metabolism results in defective Disc repair but does not affect normal wing development. Our data indicate the contribution of tissue interactions to tissue repair in Drosophila, as local damage to wing Discs influences fat body metabolism, and proper control of methionine metabolism in the fat body, in turn, affects wing regeneration.
Thomas B Kornberg - One of the best experts on this subject based on the ideXlab platform.
-
hedgehog produced by the drosophila wing Imaginal Disc induces distinct responses in three target tissues
Development, 2020Co-Authors: Ryo Hatori, Thomas B KornbergAbstract:Hedgehog (Hh) is an evolutionarily conserved signaling protein that has essential roles in animal development and homeostasis. We investigated Hh signaling in the region of the Drosophila wing Imaginal Disc that produces Hh and is near the tracheal air sac primordium (ASP) and myoblasts. Hh distributes in concentration gradients in the anterior compartment of the wing Disc, ASP, and myoblasts, and activates genes in each tissue. Some targets of Hh signal transduction are common to the Disc, ASP, and myoblasts, whereas others are tissue-specific. Signaling in the three tissues is cytoneme-mediated and cytoneme-dependent. Some ASP cells project cytonemes that receive both Hh and Branchless (Bnl, a Drosophila FGF), and some targets regulated by Hh signaling in the ASP are also dependent on Bnl signal transduction. We conclude that the single source of Hh in the wing Disc regulates cell type-specific responses in three Discreet target tissues.
-
hedgehog produced by the drosophila wing Imaginal Disc induces distinct expression responses in three target tissues
bioRxiv, 2020Co-Authors: Ryo Hatori, Thomas B KornbergAbstract:Hedgehog (Hh) is an evolutionarily conserved signaling protein that has essential roles in animal development and homeostasis. We investigated Hh signaling in the region of the Drosophila wing Imaginal Disc that produces Hh and is near the tracheal air sac primordium (ASP) and myoblasts. Hh distributes in concentration gradients in the wing Disc anterior compartment, ASP, and myoblasts and activates different sets of genes in each tissue. Some transcriptional targets of Hh signal transduction are common to the Disc, ASP, and myoblasts, whereas others are tissue-specific. Signaling in the three tissues is cytoneme-mediated and cytoneme-dependent. We conclude that a single source of Hh in the wing Disc regulates cell type-specific responses in three Discreet target tissues.
-
myoblast cytonemes mediate wg signaling from the wing Imaginal Disc and delta notch signaling to the air sac primordium
eLife, 2015Co-Authors: Hai Huang, Thomas B KornbergAbstract:The flight muscles, dorsal air sacs, wing blades, and thoracic cuticle of the Drosophila adult function in concert, and their progenitor cells develop together in the wing Imaginal Disc. The wing Disc orchestrates dorsal air sac development by producing decapentaplegic and fibroblast growth factor that travel via specific cytonemes in order to signal to the air sac primordium (ASP). Here, we report that cytonemes also link flight muscle progenitors (myoblasts) to Disc cells and to the ASP, enabling myoblasts to relay signaling between the Disc and the ASP. Frizzled (Fz)-containing myoblast cytonemes take up Wingless (Wg) from the Disc, and Delta (Dl)-containing myoblast cytonemes contribute to Notch activation in the ASP. Wg signaling negatively regulates Dl expression in the myoblasts. These results reveal an essential role for cytonemes in Wg and Notch signaling and for a signal relay system in the myoblasts.
-
regulation of drosophila matrix metalloprotease mmp2 is essential for wing Imaginal Disc trachea association and air sac tubulogenesis
Developmental Biology, 2009Co-Authors: Arjun Guha, Li Lin, Thomas B KornbergAbstract:The Drosophila Dorsal Air Sac Primordium (ASP) is a tracheal tube that grows toward Branchless FGF-expressing cells in the wing Imaginal Disc. We show that the ASP arises from a tracheal branch that invades the basal lamina of the Disc to juxtapose directly with Disc cells. We examined the role of matrix metalloproteases (Mmps), and found that reducing Mmp2 activity perturbed Disc-trachea association, altered peritracheal distributions of collagen IV and Perlecan, misregulated ASP growth, and abrogated development of the dorsal air sacs. Whereas the function of the membrane-tethered Mmp2 in the ASP is non-cell autonomous we find that it may have distinct tissue-specific roles in the ASP and Disc. These findings demonstrate a critical role for Mmp2 in tubulogenesis post-induction, and implicate Mmp2 in regulating dynamic and essential changes to the extracellular matrix.
-
regulation of cellular plasticity in drosophila Imaginal Disc cells by the polycomb group trithorax group and lama genes
Development, 2005Co-Authors: Ansgar Klebes, Gerold Schubiger, Anne Sustar, Katherina Kechris, Hao Li, Thomas B KornbergAbstract:Drosophila Imaginal Disc cells can switch fates by transdetermining from one determined state to another. We analyzed the expression profiles of cells induced by ectopic Wingless expression to transdetermine from leg to wing by dissecting transdetermined cells and hybridizing probes generated by linear RNA amplification to DNA microarrays. Changes in expression levels implicated a number of genes: lamina ancestor , CG12534 (a gene orthologous to mouse augmenter of liver regeneration), Notch pathway members, and the Polycomb and trithorax groups of chromatin regulators. Functional tests revealed that transdetermination was significantly affected in mutants for lama and seven different PcG and trxG genes. These results validate our methods for expression profiling as a way to analyze developmental programs, and show that modifications to chromatin structure are key to changes in cell fate. Our findings are likely to be relevant to the mechanisms that lead to disease when homologs of Wingless are expressed at abnormal levels and to the manifestation of pluripotency of stem cells.
Keita Masuko - One of the best experts on this subject based on the ideXlab platform.
-
Nuclear Lamin is required for Winged Eye-mediated transdetermination of Drosophila Imaginal Disc.
Genes to Cells, 2018Co-Authors: Keita Masuko, Naoyuki Fuse, Kanae Komaba, Rumi Nakajima, Hirofumi Furuhashi, Eriko Numao, Shoichiro KurataAbstract:Drosophila Imaginal Discs often change their cell fate under stress conditions, and this phenomenon, called transdetermination (TD), has long been a useful model for studying cell fate plasticity during regeneration. We previously identified a chromatin-associated protein, Winged Eye (Wge), which induces eye-to-wing TD upon its over-expression in eye Imaginal Discs. However, the molecular mechanism of Wge-mediated TD remains obscure. Here, we analyzed Wge-interacting proteins and found that several heterochromatin-related proteins, including a nuclear lamina protein, Lamin (Lam), were associated with Wge protein in cultured cells. Knockdown experiments revealed that Lam is indeed required for Wge-mediated eye-to-wing TD. Moreover, Wge over-expression altered the spatial organization of genomic DNA inside the cell nuclei. Accordingly, we suggest that Wge interacts with Lam to link some genomic regions with the nuclear periphery and regulates chromatin dynamics in Imaginal Disc TD.
-
winged eye induces transdetermination of drosophila Imaginal Disc by acting in concert with a histone methyltransferase su var 3 9
Cell Reports, 2018Co-Authors: Keita Masuko, Tomonori Katsuyama, Naoyuki Fuse, Kanae Komaba, Rumi Nakajima, Hirofumi Furuhashi, Shoichiro KurataAbstract:Drosophila Imaginal Disc cells exhibit a remarkable ability to convert cell fates in response to various perturbations, a phenomenon called transdetermination (TD). We previously identified winged eye (wge) as a factor that induces eye-to-wing TD upon overexpression in eye Imaginal Discs, but the molecular mechanisms underlying TD have remained largely unclear. Here, we found that wge induces various histone modifications and enhances the methylation of Lys9 on histone H3 (H3K9), a feature of heterochromatin. A histone methyltransferase, Su(var)3-9, is required for wge-mediated H3K9 methylation and eye-to-wing TD. Su(var)3-9 is also required for classical wound-induced TD but not for normal development, suggesting its involvement in several types of Imaginal Disc TDs. Transcriptome analysis revealed that wge represses eye identity genes independently of Su(var)3-9 and activates TD-related genes by acting together with Su(var)3-9. These findings provide new insights into diverse types of chromatin regulation at progressive steps of cell-fate conversions.
-
winged eye Induces Transdetermination of Drosophila Imaginal Disc by Acting in Concert with a Histone Methyltransferase, Su(var)3-9
Elsevier, 2018Co-Authors: Keita Masuko, Tomonori Katsuyama, Naoyuki Fuse, Kanae Komaba, Rumi Nakajima, Hirofumi Furuhashi, Shoichiro KurataAbstract:Summary: Drosophila Imaginal Disc cells exhibit a remarkable ability to convert cell fates in response to various perturbations, a phenomenon called transdetermination (TD). We previously identified winged eye (wge) as a factor that induces eye-to-wing TD upon overexpression in eye Imaginal Discs, but the molecular mechanisms underlying TD have remained largely unclear. Here, we found that wge induces various histone modifications and enhances the methylation of Lys9 on histone H3 (H3K9), a feature of heterochromatin. A histone methyltransferase, Su(var)3-9, is required for wge-mediated H3K9 methylation and eye-to-wing TD. Su(var)3-9 is also required for classical wound-induced TD but not for normal development, suggesting its involvement in several types of Imaginal Disc TDs. Transcriptome analysis revealed that wge represses eye identity genes independently of Su(var)3-9 and activates TD-related genes by acting together with Su(var)3-9. These findings provide new insights into diverse types of chromatin regulation at progressive steps of cell-fate conversions. : Drosophila Imaginal Discs switch Disc identity by a process known as transdetermination. Masuko et al. demonstrate that expression of the winged eye gene induces transdetermination through histone modifications such as H3K9-methylation. winged eye regulates expression of transdetermination-related genes via a histone methyltransferase, Su(var)3-9. Keywords: Drosophila, Imaginal Disc, transdetermination, heterochromatin, cell fate, winged eye, reprogramming, Su(var)3-
Naoyuki Fuse - One of the best experts on this subject based on the ideXlab platform.
-
Nuclear Lamin is required for Winged Eye-mediated transdetermination of Drosophila Imaginal Disc.
Genes to Cells, 2018Co-Authors: Keita Masuko, Naoyuki Fuse, Kanae Komaba, Rumi Nakajima, Hirofumi Furuhashi, Eriko Numao, Shoichiro KurataAbstract:Drosophila Imaginal Discs often change their cell fate under stress conditions, and this phenomenon, called transdetermination (TD), has long been a useful model for studying cell fate plasticity during regeneration. We previously identified a chromatin-associated protein, Winged Eye (Wge), which induces eye-to-wing TD upon its over-expression in eye Imaginal Discs. However, the molecular mechanism of Wge-mediated TD remains obscure. Here, we analyzed Wge-interacting proteins and found that several heterochromatin-related proteins, including a nuclear lamina protein, Lamin (Lam), were associated with Wge protein in cultured cells. Knockdown experiments revealed that Lam is indeed required for Wge-mediated eye-to-wing TD. Moreover, Wge over-expression altered the spatial organization of genomic DNA inside the cell nuclei. Accordingly, we suggest that Wge interacts with Lam to link some genomic regions with the nuclear periphery and regulates chromatin dynamics in Imaginal Disc TD.
-
winged eye induces transdetermination of drosophila Imaginal Disc by acting in concert with a histone methyltransferase su var 3 9
Cell Reports, 2018Co-Authors: Keita Masuko, Tomonori Katsuyama, Naoyuki Fuse, Kanae Komaba, Rumi Nakajima, Hirofumi Furuhashi, Shoichiro KurataAbstract:Drosophila Imaginal Disc cells exhibit a remarkable ability to convert cell fates in response to various perturbations, a phenomenon called transdetermination (TD). We previously identified winged eye (wge) as a factor that induces eye-to-wing TD upon overexpression in eye Imaginal Discs, but the molecular mechanisms underlying TD have remained largely unclear. Here, we found that wge induces various histone modifications and enhances the methylation of Lys9 on histone H3 (H3K9), a feature of heterochromatin. A histone methyltransferase, Su(var)3-9, is required for wge-mediated H3K9 methylation and eye-to-wing TD. Su(var)3-9 is also required for classical wound-induced TD but not for normal development, suggesting its involvement in several types of Imaginal Disc TDs. Transcriptome analysis revealed that wge represses eye identity genes independently of Su(var)3-9 and activates TD-related genes by acting together with Su(var)3-9. These findings provide new insights into diverse types of chromatin regulation at progressive steps of cell-fate conversions.
-
winged eye Induces Transdetermination of Drosophila Imaginal Disc by Acting in Concert with a Histone Methyltransferase, Su(var)3-9
Elsevier, 2018Co-Authors: Keita Masuko, Tomonori Katsuyama, Naoyuki Fuse, Kanae Komaba, Rumi Nakajima, Hirofumi Furuhashi, Shoichiro KurataAbstract:Summary: Drosophila Imaginal Disc cells exhibit a remarkable ability to convert cell fates in response to various perturbations, a phenomenon called transdetermination (TD). We previously identified winged eye (wge) as a factor that induces eye-to-wing TD upon overexpression in eye Imaginal Discs, but the molecular mechanisms underlying TD have remained largely unclear. Here, we found that wge induces various histone modifications and enhances the methylation of Lys9 on histone H3 (H3K9), a feature of heterochromatin. A histone methyltransferase, Su(var)3-9, is required for wge-mediated H3K9 methylation and eye-to-wing TD. Su(var)3-9 is also required for classical wound-induced TD but not for normal development, suggesting its involvement in several types of Imaginal Disc TDs. Transcriptome analysis revealed that wge represses eye identity genes independently of Su(var)3-9 and activates TD-related genes by acting together with Su(var)3-9. These findings provide new insights into diverse types of chromatin regulation at progressive steps of cell-fate conversions. : Drosophila Imaginal Discs switch Disc identity by a process known as transdetermination. Masuko et al. demonstrate that expression of the winged eye gene induces transdetermination through histone modifications such as H3K9-methylation. winged eye regulates expression of transdetermination-related genes via a histone methyltransferase, Su(var)3-9. Keywords: Drosophila, Imaginal Disc, transdetermination, heterochromatin, cell fate, winged eye, reprogramming, Su(var)3-