Immobilised Cell

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The Experts below are selected from a list of 3123 Experts worldwide ranked by ideXlab platform

R Borja - One of the best experts on this subject based on the ideXlab platform.

John F Kennedy - One of the best experts on this subject based on the ideXlab platform.

  • use of Immobilised biocatalysts in the processing of cheese whey
    International Journal of Biological Macromolecules, 2009
    Co-Authors: Maria R Kosseva, Parmjit S Panesar, Gurpreet Kaur, John F Kennedy
    Abstract:

    Abstract Food processing industry operations need to comply with increasingly more stringent environmental regulations related to the disposal or utilisation of by-products and wastes. These include growing restrictions on land spraying with agro-industrial wastes, and on disposal within landfill operations, and the requirements to produce end products that are stabilised and hygienic. Much of the material generated as wastes by the dairy processing industries contains components that could be utilised as substrates and nutrients in a variety of microbial/enzymatic processes, to give rise to added-value products. A good example of a waste that has received considerable attention as a source of added-value products is cheese whey. The carbohydrate reservoir of lactose (4–5%) in whey and the presence of other essential nutrients make it a good natural medium for the growth of microorganisms and a potential substrate for bioprocessing through microbial fermentation. Immobilised Cell and enzyme technology has also been applied to whey bioconversion processes to improve the economics of such processes. This review focuses upon the elaboration of a range of immobilisation techniques that have been applied to produce valuable whey-based products. A comprehensive literature survey is also provided to illustrate numerous immobilisation procedures with particular emphasis upon lactose hydrolysis, and ethanol and lactic acid production using Immobilised biocatalysts.

  • bioutilisation of whey for lactic acid production
    Food Chemistry, 2007
    Co-Authors: Parmjit S Panesar, John F Kennedy, Dina N Gandhi, Katarzyma Bunko
    Abstract:

    The disposal of whey, the liquid remaining after the separation of milk fat and casein from whole milk, is a major problem for the dairy industry, which demands simple and economical solutions. The bioconversion of lactose present in whey to valuable products has been actively explored. Since whey and whey permeates contain significant quantities of lactose, an interesting way to upgrade this effluent could be as a substrate for fermentation. Production of lactic acid through lactic acid bacteria could be a processing route for whey lactose and various attempts have been made in this direction. Immobilised Cell technology has also been applied to whey fermentation processes, to improve the economics of the process. A fermentative means of lactic acid production has advantages over chemical synthesis, as desirable optically pure lactic acid could be produced, and the demand for optically pure lactic acid has increased considerably because of its use in the production of poly(lactic acid), a biodegradable polymer, and other industrial applications. This review focuses on the various biotechnological techniques that have used whey for the production of lactic acid.

V Alonso - One of the best experts on this subject based on the ideXlab platform.

Parmjit S Panesar - One of the best experts on this subject based on the ideXlab platform.

  • use of Immobilised biocatalysts in the processing of cheese whey
    International Journal of Biological Macromolecules, 2009
    Co-Authors: Maria R Kosseva, Parmjit S Panesar, Gurpreet Kaur, John F Kennedy
    Abstract:

    Abstract Food processing industry operations need to comply with increasingly more stringent environmental regulations related to the disposal or utilisation of by-products and wastes. These include growing restrictions on land spraying with agro-industrial wastes, and on disposal within landfill operations, and the requirements to produce end products that are stabilised and hygienic. Much of the material generated as wastes by the dairy processing industries contains components that could be utilised as substrates and nutrients in a variety of microbial/enzymatic processes, to give rise to added-value products. A good example of a waste that has received considerable attention as a source of added-value products is cheese whey. The carbohydrate reservoir of lactose (4–5%) in whey and the presence of other essential nutrients make it a good natural medium for the growth of microorganisms and a potential substrate for bioprocessing through microbial fermentation. Immobilised Cell and enzyme technology has also been applied to whey bioconversion processes to improve the economics of such processes. This review focuses upon the elaboration of a range of immobilisation techniques that have been applied to produce valuable whey-based products. A comprehensive literature survey is also provided to illustrate numerous immobilisation procedures with particular emphasis upon lactose hydrolysis, and ethanol and lactic acid production using Immobilised biocatalysts.

  • bioutilisation of whey for lactic acid production
    Food Chemistry, 2007
    Co-Authors: Parmjit S Panesar, John F Kennedy, Dina N Gandhi, Katarzyma Bunko
    Abstract:

    The disposal of whey, the liquid remaining after the separation of milk fat and casein from whole milk, is a major problem for the dairy industry, which demands simple and economical solutions. The bioconversion of lactose present in whey to valuable products has been actively explored. Since whey and whey permeates contain significant quantities of lactose, an interesting way to upgrade this effluent could be as a substrate for fermentation. Production of lactic acid through lactic acid bacteria could be a processing route for whey lactose and various attempts have been made in this direction. Immobilised Cell technology has also been applied to whey fermentation processes, to improve the economics of the process. A fermentative means of lactic acid production has advantages over chemical synthesis, as desirable optically pure lactic acid could be produced, and the demand for optically pure lactic acid has increased considerably because of its use in the production of poly(lactic acid), a biodegradable polymer, and other industrial applications. This review focuses on the various biotechnological techniques that have used whey for the production of lactic acid.

Mauro Tomassetti - One of the best experts on this subject based on the ideXlab platform.

  • Toxicity order of cholanic acids using an Immobilised Cell biosensor
    Journal of Pharmaceutical and Biomedical Analysis, 1996
    Co-Authors: Luigi Campanella, D. Mastrofini, Gabriele Favero, Mauro Tomassetti
    Abstract:

    There is considerable published evidence of the use of Cells of various species to evaluate the toxicity of numerous compounds, many of pharmaceutical interest. The coupling of Cell colonies with a suitable transduction device has led to the development in recent years of toxicity biosensors based on the alteration of a process or a Cell metabolic function by the toxic substance under examination. A biosensor based on Immobilised yeast Cells (Saccharomyces cerevisiae) has been developed recently in this department for the purpose of performing a rapid toxicity test in aqueous environmental matrices. This biosensor has now been used in the toxicity screening of a number of sodium salts of conjugated and free cholanic acids. The “toxicity degree” scale, which was found by placing in decreasing order the values of the slopes of the straight lines obtained by quantifying changes in the behaviour of the respirometric curve, plotted before and after incubation, using known concentrations of cholanic acid sodium salts, was: deoxycholic acid > chenodeoxycholic acid > ursodeoxycholic acid > cholic acid, for free cholanic acids; and glycodeoxycholic acid > glycochenodeoxycholic acid > glycocholic acid, for glycocholanic acids. These values are in good agreement with published toxicity data obtained in vitro. This sensor can thus be considered to provide a valid instrument for the preliminary evaluation of the toxicity of organic compounds or drugs.