The Experts below are selected from a list of 27495 Experts worldwide ranked by ideXlab platform
Kuniaki Takata - One of the best experts on this subject based on the ideXlab platform.
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Double-Label Immunoelectron Microscopy for Studying the Colocalization of Proteins in Cultured Cells
Methods in molecular biology (Clifton N.J.), 2010Co-Authors: Haruo Hagiwara, Takeo Aoki, Takeshi Suzuki, Kuniaki TakataAbstract:Multiple label Immunoelectron Microscopy localizes and detects multiple antigens in cells and tissues. In double labeling, two kinds of primary antibodies from different animal species are used after being mixed in a single solution. To distinguish the different antigens, secondary antibodies should be labeled with colloidal gold particles of different diameter. Generally, the secondary antibody that is used for detecting the antigen with lower distribution density is labeled with smaller-sized gold particles. In this chapter, double-label Immunoelectron Microscopy of gelatin-embedded cultured cells using the cryosectioning technique is described.
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pre embedding Immunoelectron Microscopy of chemically fixed mammalian tissue culture cells
Methods of Molecular Biology, 2010Co-Authors: Haruo Hagiwara, Takeo Aoki, Takeshi Suzuki, Kuniaki TakataAbstract:: Immunoelectron Microscopy is one of the best methods for detecting and localizing protein molecules in cells and tissues. Gold particles of 1.4 nm in diameter (Nanogold) conjugated with Fab' fragments easily penetrate into the cell interior and are used for pre-embedding Immunoelectron Microscopy. To obtain a contrast for the gold label, silver enhancement of the gold particles is essential. By changing the intensity of the silver enhancement, the size of the granules can be controlled. In this chapter, we described the use of Nanogold for pre-embedding Immunoelectron Microscopy of paraformaldehyde-fixed cultured cells.
Akira Sawaguchi - One of the best experts on this subject based on the ideXlab platform.
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Immunoelectron Microscopy of cryofixed freeze-substituted mammalian tissue culture cells.
Methods in molecular biology (Clifton N.J.), 2010Co-Authors: Akira SawaguchiAbstract:Mammalian tissue cultured cells are widely used in cell biology research. Immunoelectron Microscopy is a powerful technique to define the subcellular localization of targeted antigens in the cultured cells. Cryofixation is now generally accepted as the best initial fixation step, to preserve the cellular fine structures and antigenicity. This chapter covers the practical procedures for Immunoelectron Microscopy of cryofixed freeze-substituted mammalian tissue cultured cells processed by high-pressure freezing.
Haruo Hagiwara - One of the best experts on this subject based on the ideXlab platform.
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Double-Label Immunoelectron Microscopy for Studying the Colocalization of Proteins in Cultured Cells
Methods in molecular biology (Clifton N.J.), 2010Co-Authors: Haruo Hagiwara, Takeo Aoki, Takeshi Suzuki, Kuniaki TakataAbstract:Multiple label Immunoelectron Microscopy localizes and detects multiple antigens in cells and tissues. In double labeling, two kinds of primary antibodies from different animal species are used after being mixed in a single solution. To distinguish the different antigens, secondary antibodies should be labeled with colloidal gold particles of different diameter. Generally, the secondary antibody that is used for detecting the antigen with lower distribution density is labeled with smaller-sized gold particles. In this chapter, double-label Immunoelectron Microscopy of gelatin-embedded cultured cells using the cryosectioning technique is described.
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pre embedding Immunoelectron Microscopy of chemically fixed mammalian tissue culture cells
Methods of Molecular Biology, 2010Co-Authors: Haruo Hagiwara, Takeo Aoki, Takeshi Suzuki, Kuniaki TakataAbstract:: Immunoelectron Microscopy is one of the best methods for detecting and localizing protein molecules in cells and tissues. Gold particles of 1.4 nm in diameter (Nanogold) conjugated with Fab' fragments easily penetrate into the cell interior and are used for pre-embedding Immunoelectron Microscopy. To obtain a contrast for the gold label, silver enhancement of the gold particles is essential. By changing the intensity of the silver enhancement, the size of the granules can be controlled. In this chapter, we described the use of Nanogold for pre-embedding Immunoelectron Microscopy of paraformaldehyde-fixed cultured cells.
Yoshinobu Mineyuki - One of the best experts on this subject based on the ideXlab platform.
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Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues.
Methods in molecular biology (Clifton N.J.), 2016Co-Authors: Miyuki Takeuchi, Keiji Takabe, Yoshinobu MineyukiAbstract:Cryofixation and freeze-substitution techniques provide excellent preservation of plant ultrastructure. The advantage of cryofixation is not only in structural preservation, as seen in the smooth plasma membrane, but also in the speed in arresting cell activity. Immunoelectron Microscopy reveals the subcellular localization of molecules within cells. Immunolabeling in combination with cryofixation and freeze-substitution techniques provides more detailed information on the Immunoelectron-microscopic localization of molecules in the plant cell than can be obtained from chemically fixed tissues. Here, we introduce methods for Immunoelectron Microscopy of cryofixed and freeze-substituted plant tissues.
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Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues
Methods in molecular biology (Clifton N.J.), 2010Co-Authors: Miyuki Takeuchi, Keiji Takabe, Yoshinobu MineyukiAbstract:Cryofixation and freeze-substitution techniques preserve plant ultrastructure much better than conventional chemical fixation techniques. The advantage of cryofixation is not only in structural preservation, as seen in the smooth plasma membrane, but also in the speed in arresting cell activity. Immunoelectron Microscopy reveals the subcellular localization of molecules within cells. Immunolabeling in combination with cryofixation and freeze-substitution techniques provides more detailed information on the Immunoelectron-microscopic localization of molecules in the plant cell than can be obtained from chemically fixed tissues. Here, we introduce methods for Immunoelectron Microscopy of post-embedded, cryofixed plant tissues by applying an antibody to a thin plastic resin-embedded section prepared by cryofixation followed by freeze-substitution.
Takeo Aoki - One of the best experts on this subject based on the ideXlab platform.
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Double-Label Immunoelectron Microscopy for Studying the Colocalization of Proteins in Cultured Cells
Methods in molecular biology (Clifton N.J.), 2010Co-Authors: Haruo Hagiwara, Takeo Aoki, Takeshi Suzuki, Kuniaki TakataAbstract:Multiple label Immunoelectron Microscopy localizes and detects multiple antigens in cells and tissues. In double labeling, two kinds of primary antibodies from different animal species are used after being mixed in a single solution. To distinguish the different antigens, secondary antibodies should be labeled with colloidal gold particles of different diameter. Generally, the secondary antibody that is used for detecting the antigen with lower distribution density is labeled with smaller-sized gold particles. In this chapter, double-label Immunoelectron Microscopy of gelatin-embedded cultured cells using the cryosectioning technique is described.
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pre embedding Immunoelectron Microscopy of chemically fixed mammalian tissue culture cells
Methods of Molecular Biology, 2010Co-Authors: Haruo Hagiwara, Takeo Aoki, Takeshi Suzuki, Kuniaki TakataAbstract:: Immunoelectron Microscopy is one of the best methods for detecting and localizing protein molecules in cells and tissues. Gold particles of 1.4 nm in diameter (Nanogold) conjugated with Fab' fragments easily penetrate into the cell interior and are used for pre-embedding Immunoelectron Microscopy. To obtain a contrast for the gold label, silver enhancement of the gold particles is essential. By changing the intensity of the silver enhancement, the size of the granules can be controlled. In this chapter, we described the use of Nanogold for pre-embedding Immunoelectron Microscopy of paraformaldehyde-fixed cultured cells.
