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Hoon H Sunwoo - One of the best experts on this subject based on the ideXlab platform.
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in vitro and in vivo binding activitY of chicken egg Yolk Immunoglobulin Y igY against gliadin in food matrix
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Naiyana Gujral, Raimar Löbenberg, Mavanur R. Suresh, Hoon H SunwooAbstract:Chicken egg Yolk Immunoglobulin Y (IgY) is a promising alternative for the prevention of enteric gliadin absorption, the predisposing factor of celiac disease (CD). IgY antibodY was produced from the egg Yolk of Single Comb White Leghorn chickens during the immunization period for the development of an oral immunotherapeutic agent. Here, we report the potential use of spraY dried IgY antibodY formulation using sugar protectants (mannitol, sorbitol, or microcrYstalline cellulose powder (MCCP)). The long-term stabilitY of the spraY dried egg Yolk powder formulated with 37.5% mannitol (EYP-M) preserved IgY antibodY activitY at 99.9%, which was significantlY higher than that with other protectants (p < 0.05). In a dissolution test, the EYP-M shows 82.4% IgY activitY after 2 h in simulated gastric fluid (SGF). A competitive ELISA at 50% inhibition (IC(50)) shows that 1.6 mg/mL EYP-M bound to 7.6 mg/mL and 10.5 mg/mL gliadin in SGF without and with food matrix conditions, respectivelY, whereas in simulated intestinal fluid, the formulation bound to 10 mg/mL gliadin, regardless of a food matrix. In-vivo studY: BALB/c mice fed with EYP-M and gliadin at a ratio of 1:5 (w/w) demonstrated that gliadin absorption in the gastrointestinal tract was minimal at <1%. Thus, EYP-M containing IgY antibodY maY be used in CD patients to eliminate the effects of ingested toxic gliadin.
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In-Vitro and In-Vivo Binding ActivitY of Chicken Egg Yolk Immunoglobulin Y (IgY) against Gliadin in Food Matrix
Journal of agricultural and food chemistry, 2012Co-Authors: Naiyana Gujral, Raimar Löbenberg, Mavanur R. Suresh, Hoon H SunwooAbstract:Chicken egg Yolk Immunoglobulin Y (IgY) is a promising alternative for the prevention of enteric gliadin absorption, the predisposing factor of celiac disease (CD). IgY antibodY was produced from the egg Yolk of Single Comb White Leghorn chickens during the immunization period for the development of an oral immunotherapeutic agent. Here, we report the potential use of spraY dried IgY antibodY formulation using sugar protectants (mannitol, sorbitol, or microcrYstalline cellulose powder (MCCP)). The long-term stabilitY of the spraY dried egg Yolk powder formulated with 37.5% mannitol (EYP-M) preserved IgY antibodY activitY at 99.9%, which was significantlY higher than that with other protectants (p < 0.05). In a dissolution test, the EYP-M shows 82.4% IgY activitY after 2 h in simulated gastric fluid (SGF). A competitive ELISA at 50% inhibition (IC(50)) shows that 1.6 mg/mL EYP-M bound to 7.6 mg/mL and 10.5 mg/mL gliadin in SGF without and with food matrix conditions, respectivelY, whereas in simulated intestinal fluid, the formulation bound to 10 mg/mL gliadin, regardless of a food matrix. In-vivo studY: BALB/c mice fed with EYP-M and gliadin at a ratio of 1:5 (w/w) demonstrated that gliadin absorption in the gastrointestinal tract was minimal at
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Growth inhibition of Clostridium perfringens vegetative cells and spores using chicken Immunoglobulin Y.
Journal of Food Safety, 2009Co-Authors: Min S. Song, Hoon H SunwooAbstract:ABSTRACT Egg Yolk antibodY (IgY) was isolated bY the water dilution method from the egg Yolk of chickens immunized with Clostridium perfringens vegetative cells and spores. Specific binding activitY of IgY against C. perfringens vegetative cells and spores remained relativelY high during the immunization period (up to 9 weeks). The titer of specific IgY against C. perfringens spores was 1.4-fold less than that of specific IgY against C. perfringens vegetable cells. The specific IgY powder (10 µg/mL) was found to inhibit the growth of C. perfringens vegetative cells or C. perfringens spores in a liquid medium. The difference of C. perfringens vegetative cell growth between the treatment and control groups was 8.9 × 106 colonY forming units (cfu)/mL at 8 h of incubation and 9.95 × 107 cfu/mL at 24 h of incubation. Significant cfu reductions in C. perfringens spores were also observed with specific IgY powder at 24 h of incubation. PRACTICAL APPLICATIONS IgY antibodY exerts an antimicrobial activitY against pathogens bY binding, immobilizing and consequentlY reducing or inhibiting the growth, replication or colonY forming abilitY of pathogenic bacteria; thus, it is proved to be a viable alternative for antibiotics and preservatives. In this studY, IgY against Clostridium perfringens can be used to replace chemical preservatives in food industries. Because IgY functions well at low temperature, it can be used to inhibit the growth of Clostridia which germinate in refrigerated storage conditions, thus preventing foodborne enterotoxicitY caused bY such bacteria. In practical applications, natural antimicrobial IgY antibodY can be applied to meat products for the improvement of food safetY.
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Detection of Escherichia coli O157:H7 using chicken Immunoglobulin Y.
Immunology letters, 2006Co-Authors: Hoon H Sunwoo, Welson W. Wang, Jeong S. SimAbstract:A sandwich ELISA technique was examined to detect Escherichia coli O157:H7 using chicken anti-E. coli O157:H7 IgY as the capture-antibodY and an anti-E. coli O157 mouse mAb conjugated with biotin as the detection antibodY. The anti-E. coli O157:H7 IgY was harvested from eggs laid bY hens (23 weeks of age, Single Comb White Leghorn) immunized with formalin-killed E. coli O157:H7. The IgY was purified bY water dilution methods and gel chromatographY on SephacrYl S-300 followed bY ammonium sulfate precipitation. The sensitivitY (CFU/ml) of sandwich ELISA for the E. coli O157:H7 was repeatedlY examined with 10 replicates of each sample and a standard curve was plotted. The sandwich ELISA can detect as low as 40 CFU/ml of E. coli O157:H7. The data suggest that chicken IgY-based sandwich ELISA provides a reliable, inexpensive and sensitive assaY for the detection of the food-borne pathogen E. coli O157:H7.
Itsuo Chiba - One of the best experts on this subject based on the ideXlab platform.
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anti cell associated glucosYltransferase Immunoglobulin Y suppression of salivarY mutans streptococci in healthY Young adults
Journal of the American Dental Association, 2011Co-Authors: Sa V Nguyen, Faustino C. Icatlo, Taku Nakano, Emiko Isogai, Kimiharu Hirose, Hiroyuki Mizugai, Hiroshi Isogai, Michiyo Kobayashisakamoto, Itsuo ChibaAbstract:ABSTRACT Background The authors evaluated the suppressive effects of lozenges containing egg Yolk antibodies (that is, Immunoglobulin Y [IgY]) against Streptococcus mutans cell-associated glucosYltransferase (CA-gtf) on oral colonization bY mutans streptococci (MS) in healthY Young adults. Methods In a five-daY double-masked placebo-controlled trial, Young adult participants self-administered lozenges containing anti–CA-gtf IgY (Ovalgen DC, GHEN, Gifu-CitY, Japan) or a placebo at prescribed times each daY. On the basis of bacterial colonY counts of saliva cultures, the authors analYzed the pretrial and posttrial differences in levels of MS and total anaerobic bacteria among participants in the treatment (anti–CA-gtf IgY) and placebo groups and a control group. Results SalivarY MS scores in participants in the treatment group decreased significantlY ( P Conclusions The results of the studY show that lozenges containing anti–CA-gtf IgY can suppress oral colonization bY MS in healthY Young adults. Clinical Implications Lozenges containing anti–CA-gtf IgY maY help reduce dental caries risk in humans.
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Anti–cell-associated glucosYltransferase Immunoglobulin Y suppression of salivarY mutans streptococci in healthY Young adults
Journal of the American Dental Association (1939), 2011Co-Authors: V. Nguyen, Faustino C. Icatlo, Taku Nakano, Emiko Isogai, Kimiharu Hirose, Hiroyuki Mizugai, Michiyo Kobayashi-sakamoto, Hiroshi Isogai, Itsuo ChibaAbstract:ABSTRACT Background The authors evaluated the suppressive effects of lozenges containing egg Yolk antibodies (that is, Immunoglobulin Y [IgY]) against Streptococcus mutans cell-associated glucosYltransferase (CA-gtf) on oral colonization bY mutans streptococci (MS) in healthY Young adults. Methods In a five-daY double-masked placebo-controlled trial, Young adult participants self-administered lozenges containing anti–CA-gtf IgY (Ovalgen DC, GHEN, Gifu-CitY, Japan) or a placebo at prescribed times each daY. On the basis of bacterial colonY counts of saliva cultures, the authors analYzed the pretrial and posttrial differences in levels of MS and total anaerobic bacteria among participants in the treatment (anti–CA-gtf IgY) and placebo groups and a control group. Results SalivarY MS scores in participants in the treatment group decreased significantlY ( P Conclusions The results of the studY show that lozenges containing anti–CA-gtf IgY can suppress oral colonization bY MS in healthY Young adults. Clinical Implications Lozenges containing anti–CA-gtf IgY maY help reduce dental caries risk in humans.
T. Hibi - One of the best experts on this subject based on the ideXlab platform.
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Effect of dietarY anti-urease Immunoglobulin Y on Helicobacter pYlori infection in Mongolian gerbils.
Helicobacter, 2005Co-Authors: Sachiko Nomura, Hidekazu Suzuki, Tatsuhiro Masaoka, Hiromasa Ishii, Masaki Kitajima, K. Nomoto, Kumiko Kurabayashi, T. HibiAbstract:Background and aim. Helicobacter pYlori is known to be a major pathogenic factor in the development of gastritis, peptic ulcer disease and gastric cancer. RecentlY, chicken egg Yolk Immunoglobulin Y (IgY) has been recognized as an inexpensive antibodY source for passive immunization against gastrointestinal infections. The present studY was designed to investigate the effect of anti-urease IgY on H. pYlori infection in Mongolian gerbils. Methods. H. pYlori-infected Mongolian gerbils were administered a diet containing anti-urease IgY, with or without famotidine (F). After 10 weeks, bacterial culture and measurement of the gastric mucosal mYeloperoxidase (MPO) activitY were performed. In a second experiment, another group of gerbils was started on a diet containing F + IgY a week prior to H. pYlori inoculation. After 9 weeks, these animals were examined. Results. In the H. pYlori-infected gerbils, there were no significant differences in the level of H. pYlori colonization among the different dietarY and control groups. However, the MPO activitY was significantlY decreased in the H. pYlori group administered the F + IgY diet compared with that in the H. pYlori group administered the IgY, F, or control diet. Furthermore, in the gerbils administered the F + IgY diet prior to the bacterial inoculation, inhibition of H. pYlori colonization and suppression of the elevated gastric mucosal MPO activitY were observed. Conclusions. Oral administration of urease-specific IgY not onlY inhibited H. pYlori disease activitY in H. pYlori-infected gerbils, but also prevented H. pYlori colonization in those not Yet infected. These encouraging results maY pave the waY for a novel therapeutic and prophYlactic approach in the management of H. pYlori-associated gastroduodenal disease.
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Effect of dietarY anti-Helicobacter pYlori-urease Immunoglobulin Y on Helicobacter pYlori infection
Alimentary Pharmacology & Therapeutics, 2004Co-Authors: Hidekazu Suzuki, Sachiko Nomura, Tatsuhiro Masaoka, H. Goshima, N. Kamata, Y. Kodama, Hiromasa Ishii, Masaki Kitajima, K. Nomoto, T. HibiAbstract:SUMMARY RecentlY, chicken egg Yolk was recognized as an inexpensive antibodY source, and the therapeutic usefulness of egg Yolk Immunoglobulin Y (IgY) in oral passive immunization has been investigated. Although multiple antibiotic treatments eradicate most Helicobacter pYlori (H. pYlori) infections, therapY fails in 10–15% of cases due to the development of drug resistance. ConsequentlY, it is important that new, more broadlY based therapies for the treatment of H. pYlori infection should be identified. The present studY evaluated the effect, on H. pYlori infection, of IgY prepared from egg Yolk of hens immunized with H. pYlori urease (anti-HpU IgY). Seventeen asYmptomatic volunteers diagnosed as H. pYlori-positive bY the 13 C-urea breath test (UBT) were orallY administered anti-HpU IgY for 4 weeks. Four weeks later, UBT values were significantlY decreased although no case showed H. pYlori eradication. An H. pYlori-positive 53-Year-old female gastritis patient administered anti-HpU IgY plus lansoprazole for 8 weeks showed a decrease in serum pepsinogen (PG) I and UBT values as well as an increase in the PG I/II ratio. In conclusion, anti-HpU IgY maY mitigate H. pYlori-associated gastritis and partiallY attenuate gastric urease activitY. Furthermore, anti-HpU IgY combined with antacids appears to ameliorate gastric inflammation. These encouraging results maY represent a novel approach to the management of H. pYlori-associated gastroduodenal disease.
Naiyana Gujral - One of the best experts on this subject based on the ideXlab platform.
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In-Vitro and In-Vivo Binding ActivitY of Chicken Egg Yolk Immunoglobulin Y (IgY) against Gliadin in Food Matrix
Journal of agricultural and food chemistry, 2012Co-Authors: Naiyana Gujral, Raimar Löbenberg, Mavanur R. Suresh, Hoon H SunwooAbstract:Chicken egg Yolk Immunoglobulin Y (IgY) is a promising alternative for the prevention of enteric gliadin absorption, the predisposing factor of celiac disease (CD). IgY antibodY was produced from the egg Yolk of Single Comb White Leghorn chickens during the immunization period for the development of an oral immunotherapeutic agent. Here, we report the potential use of spraY dried IgY antibodY formulation using sugar protectants (mannitol, sorbitol, or microcrYstalline cellulose powder (MCCP)). The long-term stabilitY of the spraY dried egg Yolk powder formulated with 37.5% mannitol (EYP-M) preserved IgY antibodY activitY at 99.9%, which was significantlY higher than that with other protectants (p < 0.05). In a dissolution test, the EYP-M shows 82.4% IgY activitY after 2 h in simulated gastric fluid (SGF). A competitive ELISA at 50% inhibition (IC(50)) shows that 1.6 mg/mL EYP-M bound to 7.6 mg/mL and 10.5 mg/mL gliadin in SGF without and with food matrix conditions, respectivelY, whereas in simulated intestinal fluid, the formulation bound to 10 mg/mL gliadin, regardless of a food matrix. In-vivo studY: BALB/c mice fed with EYP-M and gliadin at a ratio of 1:5 (w/w) demonstrated that gliadin absorption in the gastrointestinal tract was minimal at
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in vitro and in vivo binding activitY of chicken egg Yolk Immunoglobulin Y igY against gliadin in food matrix
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Naiyana Gujral, Raimar Löbenberg, Mavanur R. Suresh, Hoon H SunwooAbstract:Chicken egg Yolk Immunoglobulin Y (IgY) is a promising alternative for the prevention of enteric gliadin absorption, the predisposing factor of celiac disease (CD). IgY antibodY was produced from the egg Yolk of Single Comb White Leghorn chickens during the immunization period for the development of an oral immunotherapeutic agent. Here, we report the potential use of spraY dried IgY antibodY formulation using sugar protectants (mannitol, sorbitol, or microcrYstalline cellulose powder (MCCP)). The long-term stabilitY of the spraY dried egg Yolk powder formulated with 37.5% mannitol (EYP-M) preserved IgY antibodY activitY at 99.9%, which was significantlY higher than that with other protectants (p < 0.05). In a dissolution test, the EYP-M shows 82.4% IgY activitY after 2 h in simulated gastric fluid (SGF). A competitive ELISA at 50% inhibition (IC(50)) shows that 1.6 mg/mL EYP-M bound to 7.6 mg/mL and 10.5 mg/mL gliadin in SGF without and with food matrix conditions, respectivelY, whereas in simulated intestinal fluid, the formulation bound to 10 mg/mL gliadin, regardless of a food matrix. In-vivo studY: BALB/c mice fed with EYP-M and gliadin at a ratio of 1:5 (w/w) demonstrated that gliadin absorption in the gastrointestinal tract was minimal at <1%. Thus, EYP-M containing IgY antibodY maY be used in CD patients to eliminate the effects of ingested toxic gliadin.
Faustino C. Icatlo - One of the best experts on this subject based on the ideXlab platform.
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Randomized placebo-controlled clinical trial of Immunoglobulin Y as adjunct to standard supportive therapY for rotavirus-associated diarrhea among pediatric patients
Vaccine, 2012Co-Authors: Shofiqur Rahman, Takao Tsuji, Yoshikatsu Kodama, Faustino C. Icatlo, Kyoko Higo-moriguchi, Khaing Win Htun, Koki Taniguchi, Van Nguyen, Kouji UmedaAbstract:Abstract This studY aims to evaluate the effect of hYperimmune Immunoglobulin Y (IgY) against human rotavirus (HRV) among pediatric patients receiving standard supportive treatment for rotavirus-associated diarrhea mostlY with an enteric non-cholera co-pathogen in a hospital setting. Two natural HRV reassortant clinical strains ATCC VR 2273 and ATCC VR 2274 were used as mixed immunizing antigens in poultrY hens to generate anti-HRV IgY (Rotamix IgY). The Rotamix IgY was used in laboratorY and clinical studies against control or placebo IgY. The control or placebo IgY was prepared using tissue culture medium from mock-infected MA104 cell line as antigen for poultrY immunization. In vitro, Rotamix IgY exhibited multi-serotYpic cross neutralization activities along with sYnergistic effects against major global serotYpes G1, G2, G3, G4 and other human or animal rotavirus strains when compared with mono-specific IgY. Suckling mice (ICR strain) pre-treated orallY once with Rotamix IgY and then challenged with rotavirus 3 h later showed a significant dose-dependent reduction in frequencY ( p p n = 26 children per studY group. NinetY-two percent of patients in each of these groups were positive for co-infecting enteric non-cholera pathogen and all patients received standard supportive therapY for diarrhea. The patients were monitored for volume and duration of oral rehYdration fluid (ORF) and intravenous fluid (IVF) intake, dailY stool frequencY and overall duration of diarrhea, and frequencY and duration of rotavirus shedding. Compared to placebo IgY group, the Rotamix IgY group had statisticallY significant reduction in mean ORF intake ( p = 0.004), mean duration of intravenous fluid administration ( p = 0.03), mean duration of diarrhea from daY of admission ( p p = 0.05). Overall, our novel approach using oral Rotamix IgY for rotavirus-infected children mostlY with non-cholera enteric pathogen co-infection appears to be a promising, safe and effective adjunct to management of acute diarrhea in pediatric patients.
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anti cell associated glucosYltransferase Immunoglobulin Y suppression of salivarY mutans streptococci in healthY Young adults
Journal of the American Dental Association, 2011Co-Authors: Sa V Nguyen, Faustino C. Icatlo, Taku Nakano, Emiko Isogai, Kimiharu Hirose, Hiroyuki Mizugai, Hiroshi Isogai, Michiyo Kobayashisakamoto, Itsuo ChibaAbstract:ABSTRACT Background The authors evaluated the suppressive effects of lozenges containing egg Yolk antibodies (that is, Immunoglobulin Y [IgY]) against Streptococcus mutans cell-associated glucosYltransferase (CA-gtf) on oral colonization bY mutans streptococci (MS) in healthY Young adults. Methods In a five-daY double-masked placebo-controlled trial, Young adult participants self-administered lozenges containing anti–CA-gtf IgY (Ovalgen DC, GHEN, Gifu-CitY, Japan) or a placebo at prescribed times each daY. On the basis of bacterial colonY counts of saliva cultures, the authors analYzed the pretrial and posttrial differences in levels of MS and total anaerobic bacteria among participants in the treatment (anti–CA-gtf IgY) and placebo groups and a control group. Results SalivarY MS scores in participants in the treatment group decreased significantlY ( P Conclusions The results of the studY show that lozenges containing anti–CA-gtf IgY can suppress oral colonization bY MS in healthY Young adults. Clinical Implications Lozenges containing anti–CA-gtf IgY maY help reduce dental caries risk in humans.
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Anti–cell-associated glucosYltransferase Immunoglobulin Y suppression of salivarY mutans streptococci in healthY Young adults
Journal of the American Dental Association (1939), 2011Co-Authors: V. Nguyen, Faustino C. Icatlo, Taku Nakano, Emiko Isogai, Kimiharu Hirose, Hiroyuki Mizugai, Michiyo Kobayashi-sakamoto, Hiroshi Isogai, Itsuo ChibaAbstract:ABSTRACT Background The authors evaluated the suppressive effects of lozenges containing egg Yolk antibodies (that is, Immunoglobulin Y [IgY]) against Streptococcus mutans cell-associated glucosYltransferase (CA-gtf) on oral colonization bY mutans streptococci (MS) in healthY Young adults. Methods In a five-daY double-masked placebo-controlled trial, Young adult participants self-administered lozenges containing anti–CA-gtf IgY (Ovalgen DC, GHEN, Gifu-CitY, Japan) or a placebo at prescribed times each daY. On the basis of bacterial colonY counts of saliva cultures, the authors analYzed the pretrial and posttrial differences in levels of MS and total anaerobic bacteria among participants in the treatment (anti–CA-gtf IgY) and placebo groups and a control group. Results SalivarY MS scores in participants in the treatment group decreased significantlY ( P Conclusions The results of the studY show that lozenges containing anti–CA-gtf IgY can suppress oral colonization bY MS in healthY Young adults. Clinical Implications Lozenges containing anti–CA-gtf IgY maY help reduce dental caries risk in humans.
