The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Michael Torbenson - One of the best experts on this subject based on the ideXlab platform.
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Immunohistochemistry and special stains in medical liver pathology
Advances in Anatomic Pathology, 2017Co-Authors: Ian Clark, Michael TorbensonAbstract:Histochemical and immunostains are routinely used to evaluate medical liver biopsy specimens. The use of these special stains allows the identification of more clinically important information than is available on hematoxylin and eosin stains alone. These special stains are important for evaluating active and chronic injury and for establishing a specific diagnosis. The skillful use of these stains greatly improves patient care. Information on the use of special stains can be scattered in different sources, making the information hard to access. In this article, the use of special stains in medical liver biopsies is concisely reviewed.
David G Hicks - One of the best experts on this subject based on the ideXlab platform.
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recommendations for improved standardization of Immunohistochemistry
Applied Immunohistochemistry & Molecular Morphology, 2007Co-Authors: Neal S Goldstein, Stephen M Hewitt, Clive R Taylor, Hadi Yaziji, David G HicksAbstract:Immunohistochemistry (IHC) continues to suffer from variable consistency, poor reproducibility, quality assurance disparities, and the lack of standardization resulting in poor concordance, validation, and verification. This document lists the recommendations made by the Ad-Hoc Committee on Immunohi
Clive R Taylor - One of the best experts on this subject based on the ideXlab platform.
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recommendations for improved standardization of Immunohistochemistry
Applied Immunohistochemistry & Molecular Morphology, 2007Co-Authors: Neal S Goldstein, Stephen M Hewitt, Clive R Taylor, Hadi Yaziji, David G HicksAbstract:Immunohistochemistry (IHC) continues to suffer from variable consistency, poor reproducibility, quality assurance disparities, and the lack of standardization resulting in poor concordance, validation, and verification. This document lists the recommendations made by the Ad-Hoc Committee on Immunohi
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antigen retrieval Immunohistochemistry past present and future
Journal of Histochemistry and Cytochemistry, 1997Co-Authors: Shan Rong Shi, Richard J Cote, Clive R TaylorAbstract:The antigen retrieval (AR) technique, which is predominantly based on high-temperature heating of tissues, is used as a non-enzymatic pretreatment for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. It has been widely applied in pathology and analytical morphology. The existence of a growing body of literature on the AR technique raises a number of interesting issues for the further development of AR. These issues include the use of a "test battery" and the concept of "maximal retrieval" applied to the selection of optimal test protocols for the standardization of AR.
Ian Clark - One of the best experts on this subject based on the ideXlab platform.
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Immunohistochemistry and special stains in medical liver pathology
Advances in Anatomic Pathology, 2017Co-Authors: Ian Clark, Michael TorbensonAbstract:Histochemical and immunostains are routinely used to evaluate medical liver biopsy specimens. The use of these special stains allows the identification of more clinically important information than is available on hematoxylin and eosin stains alone. These special stains are important for evaluating active and chronic injury and for establishing a specific diagnosis. The skillful use of these stains greatly improves patient care. Information on the use of special stains can be scattered in different sources, making the information hard to access. In this article, the use of special stains in medical liver biopsies is concisely reviewed.
Muhammad T Idrees - One of the best experts on this subject based on the ideXlab platform.
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frequent tmprss2 erg rearrangement in prostatic small cell carcinoma detected by fluorescence in situ hybridization the superiority of fluorescence in situ hybridization over erg Immunohistochemistry
Human Pathology, 2013Co-Authors: Lindsay A Schelling, Sean R Williamson, Shaobo Zhang, Jorge L Yao, Mingsheng Wang, Jiaoti Huang, Rodolfo Montironi, Antonio Lopezbeltran, Robert E Emerson, Muhammad T IdreesAbstract:Small cell carcinoma of the prostate is both morphologically and immunohistochemically similar to small cell carcinoma of other organs such as the urinary bladder or lung. TMPRSS2-ERG gene fusion appears to be a highly specific alteration in prostatic carcinoma that is frequently shared by small cell carcinoma. In adenocarcinoma, Immunohistochemistry for the ERG protein product has been reported to correlate well with the presence of the gene fusion, although in prostatic small cell carcinoma, this relationship is not completely understood. We evaluated 54 cases of small cell carcinoma of the prostate and compared TMPRSS2-ERG gene fusion status by fluorescence in situ hybridization (FISH) to immunohistochemical staining with antibody to ERG. Of 54 cases of prostatic small cell carcinoma, 26 (48%) were positive for TMPRSS2-ERG gene fusion by FISH and 12 (22%) showed overexpression of ERG protein by Immunohistochemistry. Of the 26 cases positive by FISH, 11 were also positive for ERG protein by Immunohistochemistry. One tumor was positive by Immunohistochemistry but negative by FISH. Urinary bladder small cell carcinoma (n = 25) showed negative results by both methods; however, 2 of 14 small cell carcinomas of other organs (lung, head, and neck) showed positive Immunohistochemistry but negative FISH. Positive staining for ERG by Immunohistochemistry is present in a subset of prostatic small cell carcinomas and correlates with the presence of TMPRSS2-ERG gene fusion. Therefore, it may be useful in confirming prostatic origin when molecular testing is not accessible. However, sensitivity and specificity of ERG Immunohistochemistry in small cell carcinoma are decreased compared to FISH.
