The Experts below are selected from a list of 132 Experts worldwide ranked by ideXlab platform
F A Greco - One of the best experts on this subject based on the ideXlab platform.
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poorly differentiated carcinoma of unknown primary site clinical usefulness of Immunoperoxidase Staining
Journal of Clinical Oncology, 1991Co-Authors: John D Hainsworth, E P Wright, David H Johnson, B W Davis, F A GrecoAbstract:To assess the clinical utility of Immunoperoxidase tumor-cell Staining in patients with poorly differentiated carcinoma of unknown primary site, we performed a battery of stains on tumors from 87 patients treated between August 1978 and April 1983. Poorly differentiated carcinoma or poorly differentiated adenocarcinoma was diagnosed on the basis of light microscopic examination, and all patients were treated before the technology of Immunoperoxidase Staining was routinely used. Therefore, results of Immunoperoxidase Staining can be correlated with clinical outcome in this group of similarly treated patients with a long median follow-up. Immunoperoxidase Staining confirmed the diagnosis of poorly differentiated carcinoma in 49 patients (56%) and yielded other diagnoses in 14 patients (16%): melanoma, eight; lymphoma, four; prostatic carcinoma, one; and yolk sac carcinoma, one. In 24 patients (28%) the Immunoperoxidase Staining pattern was inconclusive; electron microscopy was usually helpful in clarifying ...
Olli Ruuskanen - One of the best experts on this subject based on the ideXlab platform.
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rapid detection of respiratory syncytial virus and influenza a virus in cell cultures by Immunoperoxidase Staining with monoclonal antibodies
Journal of Clinical Microbiology, 1990Co-Authors: Matti Waris, Thedi Ziegler, M Kivivirta, Olli RuuskanenAbstract:Peroxidase-labeled monoclonal antibodies against respiratory syncytial virus (RSV) and influenza A virus were used for Immunoperoxidase Staining (IPS) of cell cultures inoculated with nasopharyngeal aspirates. Cells were grown in 24-well plates, and specimens were inoculated by low-speed centrifugation. Cultures were incubated for 2 days at 37 degrees C and then fixed, stained, and observed by light microscopy. IPS was compared with standard virus isolation by using cultures of human diploid fibroblasts and Vero, HEp-2, and HeLa cell lines for RSV and Madin-Darby canine kidney cells for influenza A virus; these cultures were inoculated with specimens that were previously stored at -70 degrees C. Of 40 known RSV-positive specimens, 30 were found to be positive on reinoculation by both methods, and an additional 5 specimens were found to be positive by IPS only. Of 190 specimens tested for influenza A virus, 14 were positive by IPS and in tubes, and a further 8 specimens were positive by IPS only. IPS was also compared with direct detection of viral antigens in nasopharyngeal aspirates by a time-resolved fluoroimmunoassay (TR-FIA). Fresh nasopharyngeal aspirates were inoculated into human diploid fibroblasts and Madin-Darby canine kidney cells and tested for RSV and influenza A virus, respectively, by IPS. Of 110 specimens tested for RSV, 37 were positive in total, 32 were positive by IPS, and 33 were positive by TR-FIA. Of 150 specimens tested for influenza A virus, 39 were positive in total, 35 were positive by IPS, and 34 were positive by TR-FIA. IPS of cultures inoculated by centrifugation and incubated for 2 days is a sensitive method for the diagnosis of respiratory virus infections, and 24-well plates allow for the easy processing of a large number of specimens. Images
Peng Huang - One of the best experts on this subject based on the ideXlab platform.
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Immunochemical detection of meta cells of rat organs (Immunoperoxidase Staining/metal binding protein/cadmium)
2020Co-Authors: Keith G Danielson, Peng HuangAbstract:The distribution of a heavy metal binding pro- tein, metallothionein, was studied immunocytochemically by using antimetallothionein antibody and the Immunoperoxidase Staining technique on histological sections of liver, kidney, intestine, lung, and testis from cadmium-treated rats. These tissues either accu- mulate heavy metals (e.g., liver, kidney, and testis) or are exposed to metal by ingestion or inhalation (intestine and lung). Staining for metallothionein was observed intracellularly in epithelial pa- renchymal cells of the liver and kidney; all hepatocytes and most renal tubular cells stained for the protein. Accumulation of me- tallothionein was not seen in connective tissue cells surrounding either blood vessels or renal tubules. Extracellular localization of metallothionein was also observed in the liver sinusoids and within the lumina of the renal tubules, suggesting a metal transport or excretory function for this protein. Surface columnar epithelial cells of the intestinal villi indicated the presence of metallothionein but connective tissue cells of the lamina propria were negative for the protein. The granular secretory Paneth cells of the small in- testine also stained strongly for metallothionein as did respiratory epithelial cells of the lung. In the testis, metallothionein was de- tected in the Sertoli cells and interstitial cells but not within the spermatogonia. Sertoli cells are closely associated with the devel- oping spermatogonia and appear to serve a nutritive role in sper- matogenesis. Because of the secretory, absorptive, or nutritive function of the metallothionein-localizing cells in the organs stud- ied, we suggest that metallothionein may be involved in metal stor- age or transport in addition to its commonly proposed detoxifi- cation role.
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immunochemical detection of meta cells of rat organs Immunoperoxidase Staining metal binding protein cadmium
2016Co-Authors: Keith G Danielson, Peng HuangAbstract:The distribution of a heavy metal binding pro- tein, metallothionein, was studied immunocytochemically by using antimetallothionein antibody and the Immunoperoxidase Staining technique on histological sections of liver, kidney, intestine, lung, and testis from cadmium-treated rats. These tissues either accu- mulate heavy metals (e.g., liver, kidney, and testis) or are exposed to metal by ingestion or inhalation (intestine and lung). Staining for metallothionein was observed intracellularly in epithelial pa- renchymal cells of the liver and kidney; all hepatocytes and most renal tubular cells stained for the protein. Accumulation of me- tallothionein was not seen in connective tissue cells surrounding either blood vessels or renal tubules. Extracellular localization of metallothionein was also observed in the liver sinusoids and within the lumina of the renal tubules, suggesting a metal transport or excretory function for this protein. Surface columnar epithelial cells of the intestinal villi indicated the presence of metallothionein but connective tissue cells of the lamina propria were negative for the protein. The granular secretory Paneth cells of the small in- testine also stained strongly for metallothionein as did respiratory epithelial cells of the lung. In the testis, metallothionein was de- tected in the Sertoli cells and interstitial cells but not within the spermatogonia. Sertoli cells are closely associated with the devel- oping spermatogonia and appear to serve a nutritive role in sper- matogenesis. Because of the secretory, absorptive, or nutritive function of the metallothionein-localizing cells in the organs stud- ied, we suggest that metallothionein may be involved in metal stor- age or transport in addition to its commonly proposed detoxifi- cation role.
Meihsiu Chen - One of the best experts on this subject based on the ideXlab platform.
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Immunoperoxidase Staining in the differential diagnosis of parathyroid from thyroid origin in fine needle aspirates of suspected parathyroid lesions
Acta Cytologica, 1998Co-Authors: Tienchun Chang, Chinchia Tung, Yunglien Hsiao, Meihsiu ChenAbstract:OBJECTIVE: To determine whether Immunoperoxidase Staining could be used for differential diagnosis of parathyroid from thyroid origin in fine needle aspirates of suspected parathyroid lesions. STUDY DESIGN: Immunoperoxidase Staining of parathyroid hormone and thyroglobulin was performed on aspirated smears from 10 patients with parathyroid lesions (6 of 10 patients also associated with thyroid lesions) and 10 patients with thyroid lesions. All of them had surgical tissue proof of the diagnosis. RESULTS: Aspirated smears of six patients with parathyroid lesions had strong to moderate Staining for parathyroid hormone and negative Staining for thyroglobulin. Another four patients with parathyroid lesions had equivocal Staining for parathyroid hormone and negative Staining for thyroglobulin. All aspirated smears from the 16 thyroid lesions had negative Staining for parathyroid hormone and positive Staining for thyroglobulin. CONCLUSION: Immunoperoxidase Staining of parathyroid hormone and thyroglobulin, done for each suspected parathyroid lesion, was helpful in the differential diagnosis of parathyroid vs. thyroid origin.
John D Hainsworth - One of the best experts on this subject based on the ideXlab platform.
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poorly differentiated carcinoma of unknown primary site clinical usefulness of Immunoperoxidase Staining
Journal of Clinical Oncology, 1991Co-Authors: John D Hainsworth, E P Wright, David H Johnson, B W Davis, F A GrecoAbstract:To assess the clinical utility of Immunoperoxidase tumor-cell Staining in patients with poorly differentiated carcinoma of unknown primary site, we performed a battery of stains on tumors from 87 patients treated between August 1978 and April 1983. Poorly differentiated carcinoma or poorly differentiated adenocarcinoma was diagnosed on the basis of light microscopic examination, and all patients were treated before the technology of Immunoperoxidase Staining was routinely used. Therefore, results of Immunoperoxidase Staining can be correlated with clinical outcome in this group of similarly treated patients with a long median follow-up. Immunoperoxidase Staining confirmed the diagnosis of poorly differentiated carcinoma in 49 patients (56%) and yielded other diagnoses in 14 patients (16%): melanoma, eight; lymphoma, four; prostatic carcinoma, one; and yolk sac carcinoma, one. In 24 patients (28%) the Immunoperoxidase Staining pattern was inconclusive; electron microscopy was usually helpful in clarifying ...
