The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform
Aashish K Bansal - One of the best experts on this subject based on the ideXlab platform.
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Impression Cytology-proven corneal stem cell deficiency in patients after surgeries involving the limbus.
Cornea, 2001Co-Authors: Mittanamalli S Sridhar, Geeta K Vemuganti, Aashish K BansalAbstract:PURPOSE: To report three cases of limbal stem cell deficiency (confirmed by Impression Cytology) that followed multiple pterygium surgeries and therapeutic penetrating keratoplasty. METHODS: The first case, after multiple pterygium surgeries, presented with corneal scarring and thickened epithelium with vascularization sparing the central cornea in the right eye and involving the entire cornea in the left eye. The second case presented with superficial scarring and extensive vascularization after failed therapeutic graft performed for a large perforated corneal ulcer. The third case was a clear graft performed for a progressing fungal ulcer with signs of conjunctivalization inferotemporally. Limbal stem cell deficiency was clinically suspected in all of these cases. RESULTS: Goblet cells with mucin globules were found on the corneal surface by Impression Cytology in all three cases. CONCLUSIONS: We report three cases of limbal stem cell deficiency (proven by Impression Cytology) that followed multiple pterygium surgeries and therapeutic penetrating keratoplasty. Surgical insult to the limbus is the predisposing factor for stem cell damage in these cases. Involvement of the limbus by infection and use of intensive medications are probable contributing factors for stem cell damage in cases of therapeutic penetrating keratoplasty.
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Impression Cytology-proven corneal stem cell deficiency in patients after surgeries involving the limbus.
Cornea, 2001Co-Authors: Mittanamalli S Sridhar, Geeta K Vemuganti, Aashish K BansalAbstract:PURPOSE: To report three cases of limbal stem cell deficiency (confirmed by Impression Cytology) that followed multiple pterygium surgeries and therapeutic penetrating keratoplasty. METHODS: The first case, after multiple pterygium surgeries, presented with corneal scarring and thickened epithelium with vascularization sparing the central cornea in the right eye and involving the entire cornea in the left eye. The second case presented with superficial scarring and extensive vascularization after failed therapeutic graft performed for a large perforated corneal ulcer. The third case was a clear graft performed for a progressing fungal ulcer with signs of conjunctivalization inferotemporally. Limbal stem cell deficiency was clinically suspected in all of these cases. RESULTS: Goblet cells with mucin globules were found on the corneal surface by Impression Cytology in all three cases. CONCLUSIONS: We report three cases of limbal stem cell deficiency (proven by Impression Cytology) that followed multiple pterygium surgeries and therapeutic penetrating keratoplasty. Surgical insult to the limbus is the predisposing factor for stem cell damage in these cases. Involvement of the limbus by infection and use of intensive medications are probable contributing factors for stem cell damage in cases of therapeutic penetrating keratoplasty.
Mittanamalli S Sridhar - One of the best experts on this subject based on the ideXlab platform.
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Impression Cytology-proven corneal stem cell deficiency in patients after surgeries involving the limbus.
Cornea, 2001Co-Authors: Mittanamalli S Sridhar, Geeta K Vemuganti, Aashish K BansalAbstract:PURPOSE: To report three cases of limbal stem cell deficiency (confirmed by Impression Cytology) that followed multiple pterygium surgeries and therapeutic penetrating keratoplasty. METHODS: The first case, after multiple pterygium surgeries, presented with corneal scarring and thickened epithelium with vascularization sparing the central cornea in the right eye and involving the entire cornea in the left eye. The second case presented with superficial scarring and extensive vascularization after failed therapeutic graft performed for a large perforated corneal ulcer. The third case was a clear graft performed for a progressing fungal ulcer with signs of conjunctivalization inferotemporally. Limbal stem cell deficiency was clinically suspected in all of these cases. RESULTS: Goblet cells with mucin globules were found on the corneal surface by Impression Cytology in all three cases. CONCLUSIONS: We report three cases of limbal stem cell deficiency (proven by Impression Cytology) that followed multiple pterygium surgeries and therapeutic penetrating keratoplasty. Surgical insult to the limbus is the predisposing factor for stem cell damage in these cases. Involvement of the limbus by infection and use of intensive medications are probable contributing factors for stem cell damage in cases of therapeutic penetrating keratoplasty.
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Impression Cytology-proven corneal stem cell deficiency in patients after surgeries involving the limbus.
Cornea, 2001Co-Authors: Mittanamalli S Sridhar, Geeta K Vemuganti, Aashish K BansalAbstract:PURPOSE: To report three cases of limbal stem cell deficiency (confirmed by Impression Cytology) that followed multiple pterygium surgeries and therapeutic penetrating keratoplasty. METHODS: The first case, after multiple pterygium surgeries, presented with corneal scarring and thickened epithelium with vascularization sparing the central cornea in the right eye and involving the entire cornea in the left eye. The second case presented with superficial scarring and extensive vascularization after failed therapeutic graft performed for a large perforated corneal ulcer. The third case was a clear graft performed for a progressing fungal ulcer with signs of conjunctivalization inferotemporally. Limbal stem cell deficiency was clinically suspected in all of these cases. RESULTS: Goblet cells with mucin globules were found on the corneal surface by Impression Cytology in all three cases. CONCLUSIONS: We report three cases of limbal stem cell deficiency (proven by Impression Cytology) that followed multiple pterygium surgeries and therapeutic penetrating keratoplasty. Surgical insult to the limbus is the predisposing factor for stem cell damage in these cases. Involvement of the limbus by infection and use of intensive medications are probable contributing factors for stem cell damage in cases of therapeutic penetrating keratoplasty.
Kazuo Tsubota - One of the best experts on this subject based on the ideXlab platform.
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In vivo evaluation of superior limbic keratoconjunctivitis using laser scanning confocal microscopy and conjunctival Impression Cytology.
Investigative ophthalmology & visual science, 2010Co-Authors: Takashi Kojima, Yukihiro Matsumoto, Osama M. A. Ibrahim, Enrique Adan Sato, Murat Dogru, Kazuo TsubotaAbstract:PURPOSE To investigate the cytologic findings of superior bulbar conjunctiva in superior limbic keratoconjunctivitis (SLK) using laser scanning confocal microscopy and Impression Cytology in a prospective controlled study. METHODS Twenty-one eyes of 11 SLK patients (9 women, 2 men; mean age, 49.3 +/- 17.9 years) and 18 eyes of 9 control subjects (6 women, 3 men; mean age, 46.4 +/- 8.7 years) underwent tear function tests including vital stainings, Schirmer test, tear clearance test, digital confocal laser scanning microscopy, and conjunctival Impression Cytology. After confocal microscopy and Impression Cytology images were obtained, the mean individual epithelial cell area (MIECA), nucleocytoplasmic (N/C) ratio, and inflammatory cell density were analyzed. The correlation between confocal microscopy and Impression Cytology parameters was investigated. RESULTS The MIECA of SLK patients and control subjects in confocal microscopy was 786.54 +/- 463.88 microm(2) and 311.50 +/- 78.30 microm(2), respectively. The mean N/C ratio was 0.356 +/- 0.090 and 0.490 +/- 0.038, respectively. The MIECA and N/C ratio in Impression Cytology showed significant correlation with the corresponding confocal microscopy parameters (MIECA, P = 0.0028; N/C, P = 0.0051). The inflammatory cell density in confocal microscopy significantly correlated with superior bulbar conjunctival Rose-Bengal scores (P = 0.0264). CONCLUSIONS Laser scanning confocal microscopy seems to be an efficient noninvasive tool in the evaluation of phenotypic alterations of the conjunctival epithelium in SLK and may serve as an alternative for Impression Cytology. N/C ratio and inflammatory cell density appear to be two new promising parameters of in vivo confocal microscopy in the assessment of ocular surface disease in SLK.
G L Cennamo - One of the best experts on this subject based on the ideXlab platform.
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scanning electron microscopy applied to Impression Cytology for conjunctival damage from glaucoma therapy
Cornea, 2013Co-Authors: G L Cennamo, R Forte, S Del Prete, Domenico CardoneAbstract:PURPOSE To apply scanning electron microscopy to Impression Cytology (IC) to evaluate conjunctival damage in patients undergoing topical glaucoma therapy. METHODS All patients undergoing glaucoma therapy and without ocular surface disorders between September 2012 and January 2013 were enrolled. An age- and gender-matched group without glaucoma served as the control group. Conjunctival epithelium was evaluated with the ferning test (FT), Impression Cytology with light optic microscopy (ICOM), and Impression Cytology with scanning electron microscopy (ICSEM). RESULTS Twenty patients (40 eyes; 11 men and 9 women, mean age 59.9 ± 11 years) constituted the treated group. The mean duration of glaucoma therapy was 25.5 ± 13.8 months (range, 6-48 months). The mean FT, ICOM, and ICSEM grades were 2.52 ± 0.5, 2.52 ± 0.6, and 2.55 ± 0.7, respectively. Treatment duration was not significantly correlated with FT/IC grade (P = 0.1), whereas it was significantly correlated with microvilli count at ICSEM (P = 0.01). The mean FT, ICOM, and ICSEM grades were significantly lower in the control group (40 eyes; 11 men and 9 women, mean age 61.1 ± 7.12 years) than in the treated group (1.22 ± 0.4, 1.25 ± 0.4 and 1.15 ± 0.3, respectively, P < 0.001). CONCLUSIONS The FT, ICOM, and ICSEM grades were lower in eyes undergoing glaucoma therapy than in control eyes. Treatment duration was significantly associated with a reduced microvilli count at ICSEM, but not with FT or ICOM grades. Reduction of microvilli could be the first sign of cellular damage during chronic glaucoma therapy.
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Impression Cytology with scanning electron microscopy: a new method in the study of conjunctival microvilli
Eye, 2008Co-Authors: G L Cennamo, A Del Prete, R Forte, G Cafiero, S Del Prete, D MarascoAbstract:Purpose Recent studies used Impression Cytology with scanning electron microscopy (SEM) to study the conjunctival surface of bovine eyes and normal human eyes. The purpose of this study was to evaluate the use Impression Cytology and SEM (ICSEM) in patients affected by tear film abnormalities. Methods Forty-five patients were divided into three groups according to mild, moderate or severe subjective sensation of dry eye. Fifteen asymptomatic subjects served as control group. In all patients the tear film was evaluated with break-up time (BUT), Schirmer's, and Ferning test, whereas conjunctival epithelium was evaluated with Impression Cytology and optic microscopy (ICOM), and ICSEM. The Spearman rank correlation test was used to compare the outcome of these examinations with the subjective sensation of dry eye in each group, and to identify correlations among the five tests. Results ICSEM findings highly correlated with subjective dry eye sensation (Spearman correlation coefficient, 796; P
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Impression Cytology with scanning electron microscopy: a new method in the study of conjunctival microvilli.
Eye (London England), 2007Co-Authors: G L Cennamo, A Del Prete, R Forte, G Cafiero, S Del Prete, D MarascoAbstract:Recent studies used Impression Cytology with scanning electron microscopy (SEM) to study the conjunctival surface of bovine eyes and normal human eyes. The purpose of this study was to evaluate the use Impression Cytology and SEM (ICSEM) in patients affected by tear film abnormalities. Forty-five patients were divided into three groups according to mild, moderate or severe subjective sensation of dry eye. Fifteen asymptomatic subjects served as control group. In all patients the tear film was evaluated with break-up time (BUT), Schirmer's, and Ferning test, whereas conjunctival epithelium was evaluated with Impression Cytology and optic microscopy (ICOM), and ICSEM. The Spearman rank correlation test was used to compare the outcome of these examinations with the subjective sensation of dry eye in each group, and to identify correlations among the five tests. ICSEM findings highly correlated with subjective dry eye sensation (Spearman correlation coefficient, 796; P
Geeta K Vemuganti - One of the best experts on this subject based on the ideXlab platform.
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Impression Cytology-proven corneal stem cell deficiency in patients after surgeries involving the limbus.
Cornea, 2001Co-Authors: Mittanamalli S Sridhar, Geeta K Vemuganti, Aashish K BansalAbstract:PURPOSE: To report three cases of limbal stem cell deficiency (confirmed by Impression Cytology) that followed multiple pterygium surgeries and therapeutic penetrating keratoplasty. METHODS: The first case, after multiple pterygium surgeries, presented with corneal scarring and thickened epithelium with vascularization sparing the central cornea in the right eye and involving the entire cornea in the left eye. The second case presented with superficial scarring and extensive vascularization after failed therapeutic graft performed for a large perforated corneal ulcer. The third case was a clear graft performed for a progressing fungal ulcer with signs of conjunctivalization inferotemporally. Limbal stem cell deficiency was clinically suspected in all of these cases. RESULTS: Goblet cells with mucin globules were found on the corneal surface by Impression Cytology in all three cases. CONCLUSIONS: We report three cases of limbal stem cell deficiency (proven by Impression Cytology) that followed multiple pterygium surgeries and therapeutic penetrating keratoplasty. Surgical insult to the limbus is the predisposing factor for stem cell damage in these cases. Involvement of the limbus by infection and use of intensive medications are probable contributing factors for stem cell damage in cases of therapeutic penetrating keratoplasty.
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Impression Cytology-proven corneal stem cell deficiency in patients after surgeries involving the limbus.
Cornea, 2001Co-Authors: Mittanamalli S Sridhar, Geeta K Vemuganti, Aashish K BansalAbstract:PURPOSE: To report three cases of limbal stem cell deficiency (confirmed by Impression Cytology) that followed multiple pterygium surgeries and therapeutic penetrating keratoplasty. METHODS: The first case, after multiple pterygium surgeries, presented with corneal scarring and thickened epithelium with vascularization sparing the central cornea in the right eye and involving the entire cornea in the left eye. The second case presented with superficial scarring and extensive vascularization after failed therapeutic graft performed for a large perforated corneal ulcer. The third case was a clear graft performed for a progressing fungal ulcer with signs of conjunctivalization inferotemporally. Limbal stem cell deficiency was clinically suspected in all of these cases. RESULTS: Goblet cells with mucin globules were found on the corneal surface by Impression Cytology in all three cases. CONCLUSIONS: We report three cases of limbal stem cell deficiency (proven by Impression Cytology) that followed multiple pterygium surgeries and therapeutic penetrating keratoplasty. Surgical insult to the limbus is the predisposing factor for stem cell damage in these cases. Involvement of the limbus by infection and use of intensive medications are probable contributing factors for stem cell damage in cases of therapeutic penetrating keratoplasty.
