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Mary T. Fletcher - One of the best experts on this subject based on the ideXlab platform.
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Seasonal and Species Variation of the Hepatotoxin Indospicine in Australian Indigofera Legumes As Measured by UPLC-MS/MS.
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Eddie T. T. Tan, Christopher M. Materne, Richard Silcock, Bruce R. D'arcy, Rafat Al Jassim, Mary T. FletcherAbstract:Livestock industries have maintained a keen interest in pasture legumes because of the high protein content and nutritive value. Leguminous Indigofera plant species have been considered as having high feeding values to be utilized as pasture, but the occurrence of the toxic constituent indospicine in some species has restricted this utility. Indospicine has caused both primary and secondary hepatotoxicosis and also reproductive losses, but has only previously been determined in a small number of Indigofera species. This paper validates a high-throughput ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method to determine the indospicine content of various Indigofera species found in Australian pasture. Twelve species of Indigofera together with Indigastrum parviflorum plants were collected and analyzed. Of the 84 samples analyzed, *I. spicata (the asterisk indicates a naturalized species) contained the highest indospicine level (1003 ± 328 mg/kg DM, n = 4) followed by I. linnae...
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seasonal and species variation of the hepatotoxin indospicine in australian Indigofera legumes as measured by uplc ms ms
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Eddie T. T. Tan, Christopher M. Materne, Richard Silcock, Rafat Al Jassim, B R Darcy, Mary T. FletcherAbstract:Livestock industries have maintained a keen interest in pasture legumes because of the high protein content and nutritive value. Leguminous Indigofera plant species have been considered as having high feeding values to be utilized as pasture, but the occurrence of the toxic constituent indospicine in some species has restricted this utility. Indospicine has caused both primary and secondary hepatotoxicosis and also reproductive losses, but has only previously been determined in a small number of Indigofera species. This paper validates a high-throughput ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method to determine the indospicine content of various Indigofera species found in Australian pasture. Twelve species of Indigofera together with Indigastrum parviflorum plants were collected and analyzed. Of the 84 samples analyzed, *I. spicata (the asterisk indicates a naturalized species) contained the highest indospicine level (1003 ± 328 mg/kg DM, n = 4) followed by I. linnae...
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Accumulation, Persistence, and Effects of Indospicine Residues in Camels Fed Indigofera Plant.
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Eddie T. T. Tan, Bruce R. D'arcy, Rafat Al Jassim, A. Judy Cawdell-smith, Selina M. Ossedryver, Mary T. FletcherAbstract:Indospicine (l-2-amino-6-amidinohexanoic acid) is a natural hepatotoxin found in all parts of some Indigofera plants such as Indigofera linnaei and Indigofera spicata. Several studies have documented a susceptibility to this hepatotoxin in different species of animals, including cattle, sheep, dogs, and rats, which are associated with mild to severe liver disease after prolonged ingestion. However, there is little published data on the effects of this hepatotoxin in camels, even though Indigofera plants are known to be palatable to camels in central Australia. The secondary poisoning of dogs after prolonged dietary exposure to residual indospicine in camel muscle has raised additional food safety concerns. In this study, a feeding experiment was conducted to investigate the in vivo accumulation, excretion, distribution, and histopathological effects of dietary indospicine on camels. Six young camels (2-4 years old), weighing 270-390 kg, were fed daily a roughage diet consisting of Rhodes grass hay and lucerne chaff, supplemented with Indigofera and steam-flaked barley. Indigofera (I. spicata) was offered at 597 mg DM/kg body weight (bw)/day, designed to deliver 337 μg indospicine/kg bw/day, and fed for a period of 32 days. Blood and muscle biopsies were collected over the period of the study. Concentrations of indospicine in the plasma and muscle biopsy samples were quantitated by validated ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The highest concentrations in plasma (1.01 mg/L) and muscle (2.63 mg/kg fresh weight (fw)) were found at necropsy (day 33). Other tissues were also collected at necropsy, and analysis showed ubiquitous distribution of indospicine, with the highest indospicine accumulation detected in the pancreas (4.86 ± 0.56 mg/kg fw) and liver (3.60 ± 1.34 mg/kg fw), followed by the muscle, heart, and kidney. Histopathological examination of liver tissue showed multiple small foci of predominantly mononuclear inflammatory cells. After cessation of Indigofera intake, indospicine present in plasma in the remaining three camels had a longer terminal elimination half-life (18.6 days) than muscle (15.9 days), and both demonstrated monoexponential decreases.
Eddie T. T. Tan - One of the best experts on this subject based on the ideXlab platform.
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Seasonal and Species Variation of the Hepatotoxin Indospicine in Australian Indigofera Legumes As Measured by UPLC-MS/MS.
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Eddie T. T. Tan, Christopher M. Materne, Richard Silcock, Bruce R. D'arcy, Rafat Al Jassim, Mary T. FletcherAbstract:Livestock industries have maintained a keen interest in pasture legumes because of the high protein content and nutritive value. Leguminous Indigofera plant species have been considered as having high feeding values to be utilized as pasture, but the occurrence of the toxic constituent indospicine in some species has restricted this utility. Indospicine has caused both primary and secondary hepatotoxicosis and also reproductive losses, but has only previously been determined in a small number of Indigofera species. This paper validates a high-throughput ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method to determine the indospicine content of various Indigofera species found in Australian pasture. Twelve species of Indigofera together with Indigastrum parviflorum plants were collected and analyzed. Of the 84 samples analyzed, *I. spicata (the asterisk indicates a naturalized species) contained the highest indospicine level (1003 ± 328 mg/kg DM, n = 4) followed by I. linnae...
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seasonal and species variation of the hepatotoxin indospicine in australian Indigofera legumes as measured by uplc ms ms
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Eddie T. T. Tan, Christopher M. Materne, Richard Silcock, Rafat Al Jassim, B R Darcy, Mary T. FletcherAbstract:Livestock industries have maintained a keen interest in pasture legumes because of the high protein content and nutritive value. Leguminous Indigofera plant species have been considered as having high feeding values to be utilized as pasture, but the occurrence of the toxic constituent indospicine in some species has restricted this utility. Indospicine has caused both primary and secondary hepatotoxicosis and also reproductive losses, but has only previously been determined in a small number of Indigofera species. This paper validates a high-throughput ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method to determine the indospicine content of various Indigofera species found in Australian pasture. Twelve species of Indigofera together with Indigastrum parviflorum plants were collected and analyzed. Of the 84 samples analyzed, *I. spicata (the asterisk indicates a naturalized species) contained the highest indospicine level (1003 ± 328 mg/kg DM, n = 4) followed by I. linnae...
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Accumulation, Persistence, and Effects of Indospicine Residues in Camels Fed Indigofera Plant.
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Eddie T. T. Tan, Bruce R. D'arcy, Rafat Al Jassim, A. Judy Cawdell-smith, Selina M. Ossedryver, Mary T. FletcherAbstract:Indospicine (l-2-amino-6-amidinohexanoic acid) is a natural hepatotoxin found in all parts of some Indigofera plants such as Indigofera linnaei and Indigofera spicata. Several studies have documented a susceptibility to this hepatotoxin in different species of animals, including cattle, sheep, dogs, and rats, which are associated with mild to severe liver disease after prolonged ingestion. However, there is little published data on the effects of this hepatotoxin in camels, even though Indigofera plants are known to be palatable to camels in central Australia. The secondary poisoning of dogs after prolonged dietary exposure to residual indospicine in camel muscle has raised additional food safety concerns. In this study, a feeding experiment was conducted to investigate the in vivo accumulation, excretion, distribution, and histopathological effects of dietary indospicine on camels. Six young camels (2-4 years old), weighing 270-390 kg, were fed daily a roughage diet consisting of Rhodes grass hay and lucerne chaff, supplemented with Indigofera and steam-flaked barley. Indigofera (I. spicata) was offered at 597 mg DM/kg body weight (bw)/day, designed to deliver 337 μg indospicine/kg bw/day, and fed for a period of 32 days. Blood and muscle biopsies were collected over the period of the study. Concentrations of indospicine in the plasma and muscle biopsy samples were quantitated by validated ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The highest concentrations in plasma (1.01 mg/L) and muscle (2.63 mg/kg fresh weight (fw)) were found at necropsy (day 33). Other tissues were also collected at necropsy, and analysis showed ubiquitous distribution of indospicine, with the highest indospicine accumulation detected in the pancreas (4.86 ± 0.56 mg/kg fw) and liver (3.60 ± 1.34 mg/kg fw), followed by the muscle, heart, and kidney. Histopathological examination of liver tissue showed multiple small foci of predominantly mononuclear inflammatory cells. After cessation of Indigofera intake, indospicine present in plasma in the remaining three camels had a longer terminal elimination half-life (18.6 days) than muscle (15.9 days), and both demonstrated monoexponential decreases.
Ali Bain - One of the best experts on this subject based on the ideXlab platform.
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PENGARUH PENAMBAHAN TEPUNG DAUN Indigofera ZOLLINGERIANA DALAM RANSUM TERHADAP KUALITAS INTERNAL TELUR AYAM ARAB
Jurnal Ilmu dan Teknologi Peternakan Tropis, 2018Co-Authors: Asis Surajat, Muhammad Amrullah Pagala, Ali BainAbstract:ABSTRAKPenelitian bertujuan untuk mengetahui pengaruh tepung daun Indigofera (Indigofera zollingeriana) yang diberikan dalam pakan terhadap kualitas telur ayam arab. Penelitian didesain menggunakan Rancangan Acak Lengkap (RAL) dengan 4 (empat) perlakuan dan 4 (empat) ulangan. Perlakuan penelitian terdiri atas: P0 = Ransum mengandung 0% Tepung daun Indigofera, P1 = Ransum mengandung 10% Tepung daun Indigofera, P2 = Ransum mengandung 15% Tepung daun Indigofera, dan P3 = Ransum mengandung 20% Tepung daun Indigofera. Peubah yang diukur dan diamati meliputi berat kuning telur, berat putih telur, berat kerabang telur, warna kuning telur, kadar lemak, kadar protein, dan kadar kolesterol. Data yang didapatkan dianalisis menggunakan analisis ragam (ANOVA) berdasarkan RAL menggunakan aplikasi SPSS. Jika perlakuan berpengaruh nyata maka dilakukan uji lanjut dengan Uji Duncan Multiple Range Test. Berdasarkan hasil penelitian menunjukkan bahwa penambahan tepung daun Indigofera dalam pakan ayam arab, dapat mempengaruhi berat kuning telur, berat putih telur, berat kerabang telur, dan warna kuning telur ayam arab. Kata kunci :Indigofera zollingeriana, Kuning Telur, Putih Telur, dan Ayam Arab.
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PENGARUH PENAMBAHAN TEPUNG DAUN Indigofera ZOLLINGERIANA DALAM RANSUM TERHADAP PRODUKSI DAN BERAT TELUR AYAM ARAB
Jurnal Ilmu dan Teknologi Peternakan Tropis, 2018Co-Authors: Muhammad Amrullah Pagala, Ali Bain, Asis SurajatAbstract:This study aimed to evaluate the effect of using leaf flour of Indigofera zollingeriana which was added to the ration on the productivity and quality of Arab chicken eggs. This research conducted in March to May of 2018. This research conducted at the Permata Poultry Farm Group in Wua Wua Village, Kendari. This research used a completely randomized design with four (4) treatments and four (4) replications. The experimental ration which was tested consisted of: P0 = The Ration contained 0% Indigofera leaf flour, P1 = ration containied 10% Indigofera leaf flour, P2 = Ration contained 15% Indigofera leaf flour, and P3 = Ration contained 20% Indigofera leaf flour. The parameters were observed feed consumption, egg weight, feed conversion, daily egg production. The research data were analyzed by using Analysis of Variance (ANOVA) based on RAL in using Statistical Product and Service Solutions (SPSS) software. If the treatment has a significant effect, further testing will be carried out with the Duncan Multiple Range Test. The results of this study showed that the addition leaf flour of Indigofera zollingeriana in Arab chicken rations affected the feed conversion and egg weight of Arabic chicken, however Indigofera leaf flour did not affect the feed consumption and egg production of Arab chickenKeywords: Indigofera Zollingeriana, Feed conversion, Egg Production, Arabic Chicken.
Asis Surajat - One of the best experts on this subject based on the ideXlab platform.
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PENGARUH PENAMBAHAN TEPUNG DAUN Indigofera ZOLLINGERIANA DALAM RANSUM TERHADAP KUALITAS INTERNAL TELUR AYAM ARAB
Jurnal Ilmu dan Teknologi Peternakan Tropis, 2018Co-Authors: Asis Surajat, Muhammad Amrullah Pagala, Ali BainAbstract:ABSTRAKPenelitian bertujuan untuk mengetahui pengaruh tepung daun Indigofera (Indigofera zollingeriana) yang diberikan dalam pakan terhadap kualitas telur ayam arab. Penelitian didesain menggunakan Rancangan Acak Lengkap (RAL) dengan 4 (empat) perlakuan dan 4 (empat) ulangan. Perlakuan penelitian terdiri atas: P0 = Ransum mengandung 0% Tepung daun Indigofera, P1 = Ransum mengandung 10% Tepung daun Indigofera, P2 = Ransum mengandung 15% Tepung daun Indigofera, dan P3 = Ransum mengandung 20% Tepung daun Indigofera. Peubah yang diukur dan diamati meliputi berat kuning telur, berat putih telur, berat kerabang telur, warna kuning telur, kadar lemak, kadar protein, dan kadar kolesterol. Data yang didapatkan dianalisis menggunakan analisis ragam (ANOVA) berdasarkan RAL menggunakan aplikasi SPSS. Jika perlakuan berpengaruh nyata maka dilakukan uji lanjut dengan Uji Duncan Multiple Range Test. Berdasarkan hasil penelitian menunjukkan bahwa penambahan tepung daun Indigofera dalam pakan ayam arab, dapat mempengaruhi berat kuning telur, berat putih telur, berat kerabang telur, dan warna kuning telur ayam arab. Kata kunci :Indigofera zollingeriana, Kuning Telur, Putih Telur, dan Ayam Arab.
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PENGARUH PENAMBAHAN TEPUNG DAUN Indigofera ZOLLINGERIANA DALAM RANSUM TERHADAP PRODUKSI DAN BERAT TELUR AYAM ARAB
Jurnal Ilmu dan Teknologi Peternakan Tropis, 2018Co-Authors: Muhammad Amrullah Pagala, Ali Bain, Asis SurajatAbstract:This study aimed to evaluate the effect of using leaf flour of Indigofera zollingeriana which was added to the ration on the productivity and quality of Arab chicken eggs. This research conducted in March to May of 2018. This research conducted at the Permata Poultry Farm Group in Wua Wua Village, Kendari. This research used a completely randomized design with four (4) treatments and four (4) replications. The experimental ration which was tested consisted of: P0 = The Ration contained 0% Indigofera leaf flour, P1 = ration containied 10% Indigofera leaf flour, P2 = Ration contained 15% Indigofera leaf flour, and P3 = Ration contained 20% Indigofera leaf flour. The parameters were observed feed consumption, egg weight, feed conversion, daily egg production. The research data were analyzed by using Analysis of Variance (ANOVA) based on RAL in using Statistical Product and Service Solutions (SPSS) software. If the treatment has a significant effect, further testing will be carried out with the Duncan Multiple Range Test. The results of this study showed that the addition leaf flour of Indigofera zollingeriana in Arab chicken rations affected the feed conversion and egg weight of Arabic chicken, however Indigofera leaf flour did not affect the feed consumption and egg production of Arab chickenKeywords: Indigofera Zollingeriana, Feed conversion, Egg Production, Arabic Chicken.
Aldila Din Pangawikan - One of the best experts on this subject based on the ideXlab platform.
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Pemanfaatan daun Indigofera zollingeriana sebagai bahan pakan ikan patin (Pangasius sp.)
DEPIK, 2019Co-Authors: Retno Cahya Mukti, Danang Yonarta, Aldila Din PangawikanAbstract:Abstract. The feed is one of the components that support the growth and survival in fish culture. One of the alternative feed ingredients which could use as other feed ingredient was Indigofera zollingeriana leaves. The aims of this research was to determine the use of Indigofera zollingeriana leaves on the growth performance of Patin (Pangasius sp.). This research consisted of five treatments and three replications. Indigofera zollingeriana leaves were used in the diet amount of P0 (0%) (without Indigofera zollingeriana leaves addition ), P1 (10% Indigofera zollingeriana leaves addition), P2 (20% Indigofera zollingeriana leaves addition), P3 (30% Indigofera zollingeriana leaves addition), and P4 (40% Indigofera zollingeriana leaves addition). The result showed that the use of Indigofera zollingeriana leaves had a significant effect on the fish growth performance. The use of 20% zollingeriana Indigofera produced the best results of the absolute weight of 2.15 g, 1.00% daily growth rate and feed efficiency of 44.60%. Keywords: Patin, feed ingredient, Indigofera zollingeriana leave Abstrak. Pakan merupakan komponen yang menunjang pertumbuhan dan kelangsungan hidup dalam budidaya ikan. Salah satu bahan baku alternatif yang dapat digunakan sebagai bahan pakan ikan yaitu daun Indigofera zollingeriana. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan daun Indigofera zollingeriana terhadap kinerja pertumbuhan pada ikan patin. Penelitian ini terdiri dari lima perlakuan dan tiga ulangan. Perlakuan penggunaan daun Indigofera zollingeriana terdiri dari P0(0%) (tanpa penambahan daun Indigofera zollingeriana), P1 (penambahan daun Indigofera zollingeriana 10%), P2 (penambahan daun Indigofera zollingeriana 20%), P3 (penambahan daun Indigofera zollingeriana 30%), dan P4 (penambahan daun Indigofera zollingeriana 40%). Hasil penelitian menunjukkan bahwa penambahan daun Indigofera zollingeriana menunjukkan kinerja pertumbuhan pada ikan patin. Penambahan Indigofera zollingeriana sebanyak 20% menghasilkan hasil terbaik yaitu pertumbuhan bobot mutlak sebesar 2,15 g, laju pertumbuhan harian 1,00% serta efisiensi pakan sebesar 44,60%. Kata Kunci: Bahan pakan, daun Indigofera zollingeriana, ikan patin
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Pemanfaatan daun Indigofera zollingeriana sebagai bahan pakan ikan patin (Pangasius sp.)
Syiah Kuala University, 2019Co-Authors: Retno Cahya Mukti, Danang Yonarta, Aldila Din PangawikanAbstract:Abstract. Feed is one of the components that support the growth and survival in fish culture. One of the alternative feed ingredients which could use as other feed ingredient was Indigofera zollingeriana leaves. The aims of this research was to determine the use of Indigofera zollingeriana leaves on the growth performance to catfish. This research are consists of five treatments and three replications. The applications are using Indigofera zollingeriana leaves in the diet amount of P0 (0% without Indigofera zollingeriana leaves addition), P1 (10% Indigofera zollingeriana leaves addition), P2 (20% Indigofera zollingeriana leaves addition), P3 (30% Indigofera zollingeriana leaves addition), and P4 (40% Indigofera zollingeriana leaves addition). The result of this study was the use of Indigofera zollingeriana leaves has an effect to the catfish growth performance. The use of 20% zollingeriana Indigofera produce the best results is the growth of the absolute weight of 2.15 g, 1.00% daily growth rate and feed efficiency of 44.60%. Keywords: Catfish, feed ingredient, Indigofera zollingeriana leav
