The Experts below are selected from a list of 14922 Experts worldwide ranked by ideXlab platform
Richard W Gross - One of the best experts on this subject based on the ideXlab platform.
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quantitative analysis and molecular species fingerprinting of triacylglyceride molecular species directly from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry
Analytical Biochemistry, 2001Co-Authors: Richard W GrossAbstract:Abstract Herein we describe a rapid, simple, and reliable method for the quantitative analysis and molecular species fingerprinting of triacylglycerides (Tag) directly from chloroform extracts of biological samples. Previous attempts at direct Tag quantitation by positive-ion electrospray ionization mass spectrometry (ESI/MS) were confounded by the presence of overlapping peaks from choline glycerophospholipids requiring chromatographic separation of lipid extracts prior to ESI/MS analyses. By exploiting the rapid loss of phosphocholine from choline glycerophospholipids, in conjunction with neutral-loss scanning for Individual fatty acids, overlapping peaks in the ESI mass spectrum were deconvoluted generating a detailed molecular species fingerprint of Individual Tag molecular species directly from chloroform extracts of biological samples. This method readily detects as little as 0.1 pmol of each Tag molecular species from chloroform extracts and is linear over a 1000-fold dynamic range. The sensitivity of Individual Tag molecular species to ESI/MS/MS analyses correlated with the unsaturation index and inversely correlated with total aliphatic chain length of Tag. An algorithm was developed which identifies sensitivity factors, thereby allowing the rapid quantitation and molecular species fingerprinting of Tag molecular species directly from chloroform extracts of biological samples.
Shawn C Owen - One of the best experts on this subject based on the ideXlab platform.
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split enzyme fragment as a single affinity Tag that enables protein expression purification and functional assays
Biotechnology and Bioengineering, 2019Co-Authors: Sun Jin Kim, Samuel T Hatch, Andrew S Dixon, Shawn C OwenAbstract:Expressing, isolating, and characterizing recombinant proteins is crucial to many disciplines within the biological sciences. Different molecular Tagging technologies have been developed to enable each Individual step of protein production, from expression through purification and characterization. Monitoring the entire production process requires multiple Tags or molecular interactions, because no Individual Tag has provided the comprehensive breadth of utility. An ideal molecular Tag is small and does not interrupt expression, solubility, folding or function of the protein being purified and can be used throughout the production process. We adapted and integrated a split-luciferase system (NanoBiT®, Promega ®) to perform the range of techniques essential to protein production. We developed a simple method to monitor protein expression in real time to optimize expression conditions. We constructed a novel affinity chromatography system using the split-luciferase system to enable purification. We adapted western blot analysis, enzyme-linked immunosorbent assay, and cell-based bioassay to characterize the expressed proteins. Our results demonstrate that a single-Tag can fulfill all aspects needed throughout protein production.
Sun Jin Kim - One of the best experts on this subject based on the ideXlab platform.
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split enzyme fragment as a single affinity Tag that enables protein expression purification and functional assays
Biotechnology and Bioengineering, 2019Co-Authors: Sun Jin Kim, Samuel T Hatch, Andrew S Dixon, Shawn C OwenAbstract:Expressing, isolating, and characterizing recombinant proteins is crucial to many disciplines within the biological sciences. Different molecular Tagging technologies have been developed to enable each Individual step of protein production, from expression through purification and characterization. Monitoring the entire production process requires multiple Tags or molecular interactions, because no Individual Tag has provided the comprehensive breadth of utility. An ideal molecular Tag is small and does not interrupt expression, solubility, folding or function of the protein being purified and can be used throughout the production process. We adapted and integrated a split-luciferase system (NanoBiT®, Promega ®) to perform the range of techniques essential to protein production. We developed a simple method to monitor protein expression in real time to optimize expression conditions. We constructed a novel affinity chromatography system using the split-luciferase system to enable purification. We adapted western blot analysis, enzyme-linked immunosorbent assay, and cell-based bioassay to characterize the expressed proteins. Our results demonstrate that a single-Tag can fulfill all aspects needed throughout protein production.
Adwitiya Jain - One of the best experts on this subject based on the ideXlab platform.
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location estimation in a 3d environment using rfid Tags mutliple Tag readers with known location and concept of trilateration is used to estimate the physical location of an Individual Tag
2008Co-Authors: Adwitiya JainAbstract:RFID Tag location in a 3D environment is investigated. The location of the Tag with unknown coordinates can be estimated with certain accuracy. However, accuracy can be improved using the knowledge based on measurement of additional reference Tags with known location. This book studies the mathematical formulation and practical realization of location sensing using RFID Tags. Deviating from the standard use of RFID technology which employs one Tag reader to identify presence of Tag, multiple Tag reader with known location are used to estimate the physical location of an Individual Tag, with/without the help of few reference Tag with known location. Additionally, concept of trilateration has also been extended for tracking the Tag on unknown location without use of reference Tags. It was demonstrated that both approaches were proved to be cost-effective technique and estimation of the location of a specific Tag has been achieve with sufficient accuracy.
Samuel T Hatch - One of the best experts on this subject based on the ideXlab platform.
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split enzyme fragment as a single affinity Tag that enables protein expression purification and functional assays
Biotechnology and Bioengineering, 2019Co-Authors: Sun Jin Kim, Samuel T Hatch, Andrew S Dixon, Shawn C OwenAbstract:Expressing, isolating, and characterizing recombinant proteins is crucial to many disciplines within the biological sciences. Different molecular Tagging technologies have been developed to enable each Individual step of protein production, from expression through purification and characterization. Monitoring the entire production process requires multiple Tags or molecular interactions, because no Individual Tag has provided the comprehensive breadth of utility. An ideal molecular Tag is small and does not interrupt expression, solubility, folding or function of the protein being purified and can be used throughout the production process. We adapted and integrated a split-luciferase system (NanoBiT®, Promega ®) to perform the range of techniques essential to protein production. We developed a simple method to monitor protein expression in real time to optimize expression conditions. We constructed a novel affinity chromatography system using the split-luciferase system to enable purification. We adapted western blot analysis, enzyme-linked immunosorbent assay, and cell-based bioassay to characterize the expressed proteins. Our results demonstrate that a single-Tag can fulfill all aspects needed throughout protein production.