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Casamayor, Emilio O. - One of the best experts on this subject based on the ideXlab platform.
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Ecological and metabolic thresholds in the bacterial, protist and fungal microbiome of ephemeral Saline Lakes (Monegros Desert, Spain)
'Springer Science and Business Media LLC', 2021Co-Authors: Menéndez-serra Mateu, Triadó-margarit Xavier, Casamayor, Emilio O.Abstract:We studied the 16S and 18S rRNA genes of the bacterial, protist, and fungal microbiomes of 131 samples collected in 14 ephemeral small Inland Lakes located in the endorheic area of the Monegros Desert (NE Spain). The sampling covered different temporal flooding/desiccation cycles that created natural salinity gradients between 0.1% (w/v) and salt saturation. We aimed to test the hypothesis of a lack of competitive advantage for microorganisms using the “salt-in” strategy in highly fluctuating hyperSaline environments where temperature and salinity transitions widely vary within short time periods, as in ephemeral Inland Lakes. Overall, 5653 bacterial zOTUs and 2658 eukaryal zOTUs were detected heterogeneously distributed with signif- icant variations on taxonomy and general energy-yielding metabolisms and trophic strategies along the gradient. We observed a more diverse bacterial assembly than initially expected at extreme salinities and a lack of dominance of a few “salt-in” organisms. Microbial thresholds were unveiled for these highly fluctuating hyperSaline environments with high selective pressures. We conclude that the extremely high dynamism observed in the ephemeral Lakes of Monegros may have given a competitive advantage for more versatile ("salt-out") organisms compared to those better adapted to stable high salinities usually more common in solar salterns. Ephemeral Inland Saline Lakes offered a well-suited natural framework for highly detailed evolutionary and ecological studies.Funding was provided by grant BRIDGES CGL2015-69043-P and INTERACTOMA RTI2018-101205-B-I00 fromthe SpanishAgency of Research (AEI-MICINN) and European funding (ERDF) to EOC. MMS was funded by the Spanish FPI PhD scholarships program (BRIDGES-MINECO).Peer reviewe
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Ecological and Metabolic Thresholds in the Bacterial, Protist, and FungalMicrobiome of Ephemeral Saline Lakes (Monegros Desert, Spain)
'Springer Science and Business Media LLC', 2021Co-Authors: Menéndez-serra Mateu, Triadó-margarit Xavier, Casamayor, Emilio O.Abstract:Este artículo contiene 12 páginas, 5 figuras.We studied the 16S and 18S rRNA genes of the bacterial, protist, and fungal microbiomes of 131 samples collected in 14 ephemeral small Inland Lakes located in the endorheic area of the Monegros Desert (NE Spain). The sampling covered different temporal flooding/desiccation cycles that created natural salinity gradients between 0.1% (w/v) and salt saturation.We aimed to test the hypothesis of a lack of competitive advantage for microorganisms using the “salt-in” strategy in highly fluctuating hyperSaline environments where temperature and salinity transitions widely vary within short time periods, as in ephemeral Inland Lakes. Overall, 5653 bacterial zOTUs and 2658 eukaryal zOTUs were detected heterogeneously distributed with significant variations on taxonomy and general energy-yielding metabolisms and trophic strategies along the gradient. We observed a more diverse bacterial assembly than initially expected at extreme salinities and a lack of dominance of a few “salt-in” organisms. Microbial thresholds were unveiled for these highly fluctuating hyperSaline environments with high selective pressures. We conclude that the extremely high dynamism observed in the ephemeral Lakes of Monegros may have given a competitive advantage for more versatile ("salt-out") organisms compared to those better adapted to stable high salinities usually more common in solar salterns. Ephemeral Inland Saline Lakes offered a well-suited natural framework for highly detailed evolutionary and ecological studies.Funding was provided by grant BRIDGES CGL2015-69043-P and INTERACTOMA RTI2018-101205-B-I00 from the SpanishAgency of Research (AEI-MICINN) and European funding (ERDF) to EOC. MMS was funded by the Spanish FPI PhD scholarships program (BRIDGES-MINECO).Peer reviewe
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Microbial biodiversity in Saline shallow Lakes of the Monegros Desert, Spain
'Wiley', 2013Co-Authors: Casamayor, Emilio O., Triadó-margarit Xavier, Castañeda Del Álamo, CarmenAbstract:16 páginas, 2 tablas, 8 figuras.The Monegros desert contains one of the largest sets of Inland Saline Lakes in Europe constituting a threatened landscape of great scientific and ecological value with large number of reported endemisms. We analyzed bacteria, archaea and microbial eukaryotes from 11 Saline Lakes in winter and spring by rRNA gene fingerprinting and sequencing covering large salinity (2.7-22.1%) and temperature ranges (1.5-35.3°C). The highest ecological diversity (Shannon-Weaver index) was found in protists and the lowest in Archaea. Eukaryotes showed higher ecological diversity at intermediate salinities, whereas Bacteria and Archaea did not. The genetic diversity was broad and with remarkable novelty. The highest novelty was found in Archaea at the lowest Saline concentrations, whereas for bacteria and protists no differences were observed along the gradient. Euryarchaeota of the enigmatic group DHVEG-6 and phylotypes distantly related to well-known haloarchaea were present in several sites. Recurrent presence of bacterial phylotypes distantly related to Psychroflexus and Cryomorphaceae initially isolated from polar marine habitats, was observed. Saline Lakes contained chlorophyta, among other new groups, substantially different from green algae previously reported in marine or freshwater. The great scientific and ecological value found for macroorganisms can be extended to the idiosyncratic microbes inhabiting such unique habitat in Europe.This research was granted by projects MONEGROS CGL2004-22780-E and DARKNESS CGL2012-32747 to EOC from the Spanish Ministerio de Economía y Competitividad (MINECO), and grants 2012/GA LC 036 from the Gobierno de Aragón-La Caixa, and AGL2012- 40100 from MINECO.Peer reviewe
Mario Garciaparis - One of the best experts on this subject based on the ideXlab platform.
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phylogenetic relationships and biogeography of midwife toads discoglossidae alytes
Journal of Biogeography, 2004Co-Authors: Inigo Martinezsolano, Helena Goncalves, J W Arntzen, Mario GarciaparisAbstract:Aim To devise a robust phylogenetic hypothesis for midwife toads (Alytes: Anura: Discoglossidae) and to discuss its implications for the reconstruction of the biogeographical history of the group. Location Western Palearctic. Methods Analysis of sequences of mitochondrial DNA (cytochrome b and 16S RNA, 861 bp) and 29 characters of cranial osteology of all species and subspecies within Alytes. Results Phylogenetic analyses support a sister group relationship between Alytes dickhilleni and A. muletensis, and of this clade with A. maurus. The monophyly of A. obstetricans is controversial; in particular, the phylogenetic position of A. obstetricans almogavarii is uncertain. The estimated dates for the cladogenetic events within Alytes are congruent with those derived from independent analyses (allozymes), except for the differentiation of A. o. almogavarii and the split between A. dickhilleni and A. muletensis. Main conclusions The phylogeny based on the analysis of morphological and mtDNA data differs from previous hypotheses in the positions of A. o. almogavarii and A. maurus. Events associated with the radiation of Alytes are the formation of large Inland Saline Lakes in Iberia c. 16 Ma and the 11 ° C dramatic decrease in average annual temperature during the Middle–Late Badenian transition c. 14–13.5 Ma (A.cisternasii vs. the ancestor of the remaining clades), the structuring of the Neo-Pyrenees and the reopening of the Betic Strait c. 10–8 Ma(A. o. almogavarii vs. A. obstetricans and vs. the subgenus Baleaphryne), the opening of the Gibraltar Strait at the end of the Messinian Salinity Crisis at c. 5.3 Ma (A. maurus vs. the ancestor of the A. dickhilleni A. muletensis clade) and a transmarine colonization event at c. 3 Ma (the ancestor of A. muletensis vs. A. dickhilleni). Following the new hypothesis, A. maurus, previously considered a subspecies of A. obstetricans, deserves species status. Second, A. o. almogavarii is a well-differentiated lineage that was isolated from other A. obstetricans more than c. 5 Ma, but later lost its genetic and specific identity following secondary contact, hybridization and introgression with the main stock. The presence of a marked morphological and genetic diversity within A. obstetricans renders reconstruction of the evolutionary history of the genus more complicated than previously appreciated.
Helena Goncalves - One of the best experts on this subject based on the ideXlab platform.
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phylogenetic relationships and biogeography of midwife toads discoglossidae alytes
Journal of Biogeography, 2004Co-Authors: Inigo Martinezsolano, Helena Goncalves, J W Arntzen, Mario GarciaparisAbstract:Aim To devise a robust phylogenetic hypothesis for midwife toads (Alytes: Anura: Discoglossidae) and to discuss its implications for the reconstruction of the biogeographical history of the group. Location Western Palearctic. Methods Analysis of sequences of mitochondrial DNA (cytochrome b and 16S RNA, 861 bp) and 29 characters of cranial osteology of all species and subspecies within Alytes. Results Phylogenetic analyses support a sister group relationship between Alytes dickhilleni and A. muletensis, and of this clade with A. maurus. The monophyly of A. obstetricans is controversial; in particular, the phylogenetic position of A. obstetricans almogavarii is uncertain. The estimated dates for the cladogenetic events within Alytes are congruent with those derived from independent analyses (allozymes), except for the differentiation of A. o. almogavarii and the split between A. dickhilleni and A. muletensis. Main conclusions The phylogeny based on the analysis of morphological and mtDNA data differs from previous hypotheses in the positions of A. o. almogavarii and A. maurus. Events associated with the radiation of Alytes are the formation of large Inland Saline Lakes in Iberia c. 16 Ma and the 11 ° C dramatic decrease in average annual temperature during the Middle–Late Badenian transition c. 14–13.5 Ma (A.cisternasii vs. the ancestor of the remaining clades), the structuring of the Neo-Pyrenees and the reopening of the Betic Strait c. 10–8 Ma(A. o. almogavarii vs. A. obstetricans and vs. the subgenus Baleaphryne), the opening of the Gibraltar Strait at the end of the Messinian Salinity Crisis at c. 5.3 Ma (A. maurus vs. the ancestor of the A. dickhilleni A. muletensis clade) and a transmarine colonization event at c. 3 Ma (the ancestor of A. muletensis vs. A. dickhilleni). Following the new hypothesis, A. maurus, previously considered a subspecies of A. obstetricans, deserves species status. Second, A. o. almogavarii is a well-differentiated lineage that was isolated from other A. obstetricans more than c. 5 Ma, but later lost its genetic and specific identity following secondary contact, hybridization and introgression with the main stock. The presence of a marked morphological and genetic diversity within A. obstetricans renders reconstruction of the evolutionary history of the genus more complicated than previously appreciated.
Hairong Duan - One of the best experts on this subject based on the ideXlab platform.
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High Genetic Diversity and Novelty in Eukaryotic Plankton Assemblages Inhabiting Saline Lakes in the Qaidam Basin
2016Co-Authors: Jiali Wang, Fang Wang, Limin Chu, Hao Wang, Zhiping Zhong, Zhipei Liu, Jianyong Gao, Hairong DuanAbstract:Saline Lakes are intriguing ecosystems harboring extremely productive microbial communities in spite of their extreme environmental conditions. We performed a comprehensive analysis of the genetic diversity (18S rRNA gene) of the planktonic microbial eukaryotes (nano- and picoeukaryotes) in six different Inland Saline Lakes located in the Qaidam Basin. The novelty level are high, with about 11.23 % of the whole dataset showing,90 % identity to any previously reported sequence in GenBank. At least 4 operational taxonomic units (OTUs) in mesoSaline Lakes, while up to eighteen OTUs in hyperSaline Lakes show very low CCM and CEM scores, indicating that these sequences are highly distantly related to any existing sequence. Most of the 18S rRNA gene sequence reads obtained in investigated mesoSaline Lakes is closely related to Holozoa group (48.13%), whereas Stramenopiles (26.65%) and Alveolates (10.84%) are the next most common groups. HyperSaline Lakes in the Qaidam Basin are also dominated by Holozoa group, accounting for 26.65 % of the total number of sequence reads. Notably, Chlorophyta group are only found in high abundance in Lake Gasikule (28.00%), whereas less represented in other hyperSaline Lakes such as Gahai (0.50%) and Xiaochaidan (1.15%). Further analysis show that the compositions of planktonic eukaryotic assemblages are also most variable between different sampling sites in the same lake. Out of the parameters, four show significant correlation to this CCA: altitude, calcium, sodium and potassium concentrations. Overall, this study shows important gaps in the current knowledge about planktonic microbial eukaryote
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High Genetic Diversity and Novelty in Eukaryotic Plankton Assemblages Inhabiting Saline Lakes in the Qaidam Basin
2014Co-Authors: Jiali Wang, Fang Wang, Limin Chu, Hao Wang, Zhiping Zhong, Zhipei Liu, Jianyong Gao, Hairong DuanAbstract:Saline Lakes are intriguing ecosystems harboring extremely productive microbial communities in spite of their extreme environmental conditions. We performed a comprehensive analysis of the genetic diversity (18S rRNA gene) of the planktonic microbial eukaryotes (nano- and picoeukaryotes) in six different Inland Saline Lakes located in the Qaidam Basin. The novelty level are high, with about 11.23% of the whole dataset showing
Inigo Martinezsolano - One of the best experts on this subject based on the ideXlab platform.
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phylogenetic relationships and biogeography of midwife toads discoglossidae alytes
Journal of Biogeography, 2004Co-Authors: Inigo Martinezsolano, Helena Goncalves, J W Arntzen, Mario GarciaparisAbstract:Aim To devise a robust phylogenetic hypothesis for midwife toads (Alytes: Anura: Discoglossidae) and to discuss its implications for the reconstruction of the biogeographical history of the group. Location Western Palearctic. Methods Analysis of sequences of mitochondrial DNA (cytochrome b and 16S RNA, 861 bp) and 29 characters of cranial osteology of all species and subspecies within Alytes. Results Phylogenetic analyses support a sister group relationship between Alytes dickhilleni and A. muletensis, and of this clade with A. maurus. The monophyly of A. obstetricans is controversial; in particular, the phylogenetic position of A. obstetricans almogavarii is uncertain. The estimated dates for the cladogenetic events within Alytes are congruent with those derived from independent analyses (allozymes), except for the differentiation of A. o. almogavarii and the split between A. dickhilleni and A. muletensis. Main conclusions The phylogeny based on the analysis of morphological and mtDNA data differs from previous hypotheses in the positions of A. o. almogavarii and A. maurus. Events associated with the radiation of Alytes are the formation of large Inland Saline Lakes in Iberia c. 16 Ma and the 11 ° C dramatic decrease in average annual temperature during the Middle–Late Badenian transition c. 14–13.5 Ma (A.cisternasii vs. the ancestor of the remaining clades), the structuring of the Neo-Pyrenees and the reopening of the Betic Strait c. 10–8 Ma(A. o. almogavarii vs. A. obstetricans and vs. the subgenus Baleaphryne), the opening of the Gibraltar Strait at the end of the Messinian Salinity Crisis at c. 5.3 Ma (A. maurus vs. the ancestor of the A. dickhilleni A. muletensis clade) and a transmarine colonization event at c. 3 Ma (the ancestor of A. muletensis vs. A. dickhilleni). Following the new hypothesis, A. maurus, previously considered a subspecies of A. obstetricans, deserves species status. Second, A. o. almogavarii is a well-differentiated lineage that was isolated from other A. obstetricans more than c. 5 Ma, but later lost its genetic and specific identity following secondary contact, hybridization and introgression with the main stock. The presence of a marked morphological and genetic diversity within A. obstetricans renders reconstruction of the evolutionary history of the genus more complicated than previously appreciated.