The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform
Aine L Plant - One of the best experts on this subject based on the ideXlab platform.
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Insect Attack and wounding induce traumatic resin duct development and gene expression of pinene synthase in sitka spruce
Plant Physiology, 2003Co-Authors: Ashley Byun S Mckay, William L Hunter, Kimberleyann Godard, Shawn X Wang, Diane M Martin, Jorg Bohlmann, Aine L PlantAbstract:Conifers possess inducible terpenoid defense systems. These systems are associated with the formation of traumatic resin ducts (TRD) and are underpinned by enhanced gene expression and activity of terpene synthases (TPS), enzymes responsible for oleoresin formation. We first determined that Sitka spruce (Picea sitchensis [Bong.] Carriere) had the capacity for TRD formation by mechanically wounding representative trees. We then proceeded to investigate whether the white pine weevil (Pissodes strobi Peck.), a stem-boring Insect, can influence the expression of genes encoding monoterpene synthases (mono-tps) in Sitka spruce. We went on to compare this response with the effects of a simulated Insect Attack by drill wounding. A significant increase in mono-tps transcript level was observed in the leaders of lateral branches of weevil-Attacked and mechanically wounded trees. In this study, weevils induced a more rapid enhancement of mono-tps gene expression. A full-length Sitka spruce mono-tps cDNA (PsTPS2) was isolated, expressed in Escherichia coli, and functionally identified as (—)-pinene synthase. The recombinant (—)-pinene synthase catalyzes the formation of (—)-α-pinene and (—)-β-pinene, both of which are known constituents of stem oleoresin in Sitka spruce and increase in abundance after weevil Attack. These data suggest that increased (—)-pinene synthase gene expression is an important element of the direct defense system deployed in Sitka spruce after Insect Attack.
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Insect Attack and Wounding Induce Traumatic Resin Duct Development and Gene Expression of (—)-Pinene Synthase in Sitka Spruce
Plant Physiology, 2003Co-Authors: S. Ashley Byun Mckay, William L Hunter, Kimberleyann Godard, Shawn X Wang, Diane M Martin, Jorg Bohlmann, Aine L PlantAbstract:Conifers possess inducible terpenoid defense systems. These systems are associated with the formation of traumatic resin ducts (TRD) and are underpinned by enhanced gene expression and activity of terpene synthases (TPS), enzymes responsible for oleoresin formation. We first determined that Sitka spruce (Picea sitchensis [Bong.] Carriere) had the capacity for TRD formation by mechanically wounding representative trees. We then proceeded to investigate whether the white pine weevil (Pissodes strobi Peck.), a stem-boring Insect, can influence the expression of genes encoding monoterpene synthases (mono-tps) in Sitka spruce. We went on to compare this response with the effects of a simulated Insect Attack by drill wounding. A significant increase in mono-tps transcript level was observed in the leaders of lateral branches of weevil-Attacked and mechanically wounded trees. In this study, weevils induced a more rapid enhancement of mono-tps gene expression. A full-length Sitka spruce mono-tps cDNA (PsTPS2) was isolated, expressed in Escherichia coli, and functionally identified as (—)-pinene synthase. The recombinant (—)-pinene synthase catalyzes the formation of (—)-α-pinene and (—)-β-pinene, both of which are known constituents of stem oleoresin in Sitka spruce and increase in abundance after weevil Attack. These data suggest that increased (—)-pinene synthase gene expression is an important element of the direct defense system deployed in Sitka spruce after Insect Attack.
Corne M J Pieterse - One of the best experts on this subject based on the ideXlab platform.
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The rhizosphere microbiome and plant health
Trends in Plant Science, 2012Co-Authors: Roeland L Berendsen, Corne M J Pieterse, Peter A H M BakkerAbstract:The diversity of microbes associated with plant roots is enormous, in the order of tens of thousands of species. This complex plant-associated microbial community, also referred to as the second genome of the plant, is crucial for plant health. Recent advances in plant-microbe interactions research revealed that plants are able to shape their rhizosphere microbiome, as evidenced by the fact that different plant species host specific microbial communities when grown on the same soil. In this review, we discuss evidence that upon pathogen or Insect Attack, plants are able to recruit protective microorganisms, and enhance microbial activity to suppress pathogens in the rhizosphere. A comprehensive understanding of the mechanisms that govern selection and activity of microbial communities by plant roots will provide new opportunities to increase crop production. © 2012 Elsevier Ltd.
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signal signature and transcriptome changes of arabidopsis during pathogen and Insect Attack
Molecular Plant-microbe Interactions, 2005Co-Authors: V R Van Oosten, J A Van Pelt, L C Van Loon, Marcel Dicke, Remco M P Van Poecke, Maria J Pozo, Martin J Mueller, A J Buchala, Jeanpierre Metraux, Corne M J PieterseAbstract:Plant defenses against pathogens and Insects are regulated differentially by cross-communicating signaling pathways in which salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) play key roles. To understand how plants integrate pathogen- and Insect-induced signals into specific defense responses, we monitored the dynamics of SA, JA, and ET signaling in Arabidopsis after Attack by a set of microbial pathogens and herbivorous Insects with different modes of Attack. Arabidopsis plants were exposed to a pathogenic leaf bacterium (Pseudomonas syringae pv. tomato), a pathogenic leaf fungus (Alternaria brassicicola), tissuechewing caterpillars (Pieris rapae), cell-content-feeding thrips (Frankliniella occidentalis), or phloem-feeding aphids (Myzus persicae). Monitoring the signal signature in each plant-Attacker combination showed that the kinetics of SA, JA, and ET production varies greatly in both quantity and timing. Analysis of global gene expression profiles demonstrated that the signal signature characteristic of each Arabidopsis-Attacker combination is orchestrated into a surprisingly complex set of transcriptional alterations in which, in all cases, stress-related genes are overrepresented. Comparison of the transcript profiles revealed that consistent changes induced by pathogens and Insects with very different modes of Attack can show considerable overlap. Of all consistent changes induced by A. brassicicola, Pieris rapae, and F. occidentalis, more than 50% also were induced consistently by P. syringae. Notably, although these four Attackers all stimulated JA biosynthesis, the majority of the changes in JA-responsive gene expression were Attacker specific. All together, our study shows that SA, JA, and ET play a primary role in the orchestration of the plant’s defense response, but other regulatory mechanisms, such as pathway cross-talk or additional Attacker-induced signals, eventually shape the highly complex Attacker-specific defense response.
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signal signature of arabidopsis induced upon pathogen and Insect Attack
Biology of Plant-Microbe Interactions Vol. 4, 2004Co-Authors: V R Van Oosten, J A Van Pelt, L C Van Loon, Marcel Dicke, Corne M J PieterseAbstract:Three plant signaling molecules play a dominant role in the regulation of defences in a number of plant-Attacker model systems: salicylic acid (SA), jasmonic acid (JA) and ethylene (ET). In this study, the roles of these compounds were determined in the induced defense responses of Arabidopsis thaliana upon Attack by a set of microbial pathogens and herbivorous Insects. The production of SA, JA and ET was activated in different combinations depending on the Attacker encountered resulting in a specific signal signature. Analysis of the expression of SA-,JA-,and ET responsive marker genes showed that the signal signature nicely correlates with the expression of the marker genes in each plant-Attacker interaction. We hypothesize that the specific signal signature is involved in the activation of an optimal mix of defenses to counteract the intruder.
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signal signature in induced defense of arabidopsis upon pathogen and Insect Attack
Biology of plant-microbe interactions, 2004Co-Authors: V R Van Oosten, J A Van Pelt, L C Van Loon, Marcel Dicke, Remco M P Van Poecke, Corne M J PieterseAbstract:Three plant signaling molecules play a dominant role in the regulation of defenses in a number of plant-Attacker model systems: salicylic acid (SA), jasmonic acid (JA) and ethylene (ET). In this study, the roles of these signaling compounds were determined in the induced defense responses of Arabidopsis thaliana upon Attack by a set of microbial pathogens and herbivorous Insects. The production of SA, JA and ET was activated in different combinations depending on the Attacker encountered resulting in a specific signal signature. Analysis of the expression of SA-, JA-, and ET- responsive marker genes showed that the signal signature nicely correlates with the expression of the marker genes in each plant-Attacker interaction. We hypothesize that the specific signal signature is involved in the activation of an optimal mix of defenses to counteract the intruder.
Ashley Byun S Mckay - One of the best experts on this subject based on the ideXlab platform.
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Insect Attack and wounding induce traumatic resin duct development and gene expression of pinene synthase in sitka spruce
Plant Physiology, 2003Co-Authors: Ashley Byun S Mckay, William L Hunter, Kimberleyann Godard, Shawn X Wang, Diane M Martin, Jorg Bohlmann, Aine L PlantAbstract:Conifers possess inducible terpenoid defense systems. These systems are associated with the formation of traumatic resin ducts (TRD) and are underpinned by enhanced gene expression and activity of terpene synthases (TPS), enzymes responsible for oleoresin formation. We first determined that Sitka spruce (Picea sitchensis [Bong.] Carriere) had the capacity for TRD formation by mechanically wounding representative trees. We then proceeded to investigate whether the white pine weevil (Pissodes strobi Peck.), a stem-boring Insect, can influence the expression of genes encoding monoterpene synthases (mono-tps) in Sitka spruce. We went on to compare this response with the effects of a simulated Insect Attack by drill wounding. A significant increase in mono-tps transcript level was observed in the leaders of lateral branches of weevil-Attacked and mechanically wounded trees. In this study, weevils induced a more rapid enhancement of mono-tps gene expression. A full-length Sitka spruce mono-tps cDNA (PsTPS2) was isolated, expressed in Escherichia coli, and functionally identified as (—)-pinene synthase. The recombinant (—)-pinene synthase catalyzes the formation of (—)-α-pinene and (—)-β-pinene, both of which are known constituents of stem oleoresin in Sitka spruce and increase in abundance after weevil Attack. These data suggest that increased (—)-pinene synthase gene expression is an important element of the direct defense system deployed in Sitka spruce after Insect Attack.
Shawn X Wang - One of the best experts on this subject based on the ideXlab platform.
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Insect Attack and wounding induce traumatic resin duct development and gene expression of pinene synthase in sitka spruce
Plant Physiology, 2003Co-Authors: Ashley Byun S Mckay, William L Hunter, Kimberleyann Godard, Shawn X Wang, Diane M Martin, Jorg Bohlmann, Aine L PlantAbstract:Conifers possess inducible terpenoid defense systems. These systems are associated with the formation of traumatic resin ducts (TRD) and are underpinned by enhanced gene expression and activity of terpene synthases (TPS), enzymes responsible for oleoresin formation. We first determined that Sitka spruce (Picea sitchensis [Bong.] Carriere) had the capacity for TRD formation by mechanically wounding representative trees. We then proceeded to investigate whether the white pine weevil (Pissodes strobi Peck.), a stem-boring Insect, can influence the expression of genes encoding monoterpene synthases (mono-tps) in Sitka spruce. We went on to compare this response with the effects of a simulated Insect Attack by drill wounding. A significant increase in mono-tps transcript level was observed in the leaders of lateral branches of weevil-Attacked and mechanically wounded trees. In this study, weevils induced a more rapid enhancement of mono-tps gene expression. A full-length Sitka spruce mono-tps cDNA (PsTPS2) was isolated, expressed in Escherichia coli, and functionally identified as (—)-pinene synthase. The recombinant (—)-pinene synthase catalyzes the formation of (—)-α-pinene and (—)-β-pinene, both of which are known constituents of stem oleoresin in Sitka spruce and increase in abundance after weevil Attack. These data suggest that increased (—)-pinene synthase gene expression is an important element of the direct defense system deployed in Sitka spruce after Insect Attack.
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Insect Attack and Wounding Induce Traumatic Resin Duct Development and Gene Expression of (—)-Pinene Synthase in Sitka Spruce
Plant Physiology, 2003Co-Authors: S. Ashley Byun Mckay, William L Hunter, Kimberleyann Godard, Shawn X Wang, Diane M Martin, Jorg Bohlmann, Aine L PlantAbstract:Conifers possess inducible terpenoid defense systems. These systems are associated with the formation of traumatic resin ducts (TRD) and are underpinned by enhanced gene expression and activity of terpene synthases (TPS), enzymes responsible for oleoresin formation. We first determined that Sitka spruce (Picea sitchensis [Bong.] Carriere) had the capacity for TRD formation by mechanically wounding representative trees. We then proceeded to investigate whether the white pine weevil (Pissodes strobi Peck.), a stem-boring Insect, can influence the expression of genes encoding monoterpene synthases (mono-tps) in Sitka spruce. We went on to compare this response with the effects of a simulated Insect Attack by drill wounding. A significant increase in mono-tps transcript level was observed in the leaders of lateral branches of weevil-Attacked and mechanically wounded trees. In this study, weevils induced a more rapid enhancement of mono-tps gene expression. A full-length Sitka spruce mono-tps cDNA (PsTPS2) was isolated, expressed in Escherichia coli, and functionally identified as (—)-pinene synthase. The recombinant (—)-pinene synthase catalyzes the formation of (—)-α-pinene and (—)-β-pinene, both of which are known constituents of stem oleoresin in Sitka spruce and increase in abundance after weevil Attack. These data suggest that increased (—)-pinene synthase gene expression is an important element of the direct defense system deployed in Sitka spruce after Insect Attack.
Kimberleyann Godard - One of the best experts on this subject based on the ideXlab platform.
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Insect Attack and wounding induce traumatic resin duct development and gene expression of pinene synthase in sitka spruce
Plant Physiology, 2003Co-Authors: Ashley Byun S Mckay, William L Hunter, Kimberleyann Godard, Shawn X Wang, Diane M Martin, Jorg Bohlmann, Aine L PlantAbstract:Conifers possess inducible terpenoid defense systems. These systems are associated with the formation of traumatic resin ducts (TRD) and are underpinned by enhanced gene expression and activity of terpene synthases (TPS), enzymes responsible for oleoresin formation. We first determined that Sitka spruce (Picea sitchensis [Bong.] Carriere) had the capacity for TRD formation by mechanically wounding representative trees. We then proceeded to investigate whether the white pine weevil (Pissodes strobi Peck.), a stem-boring Insect, can influence the expression of genes encoding monoterpene synthases (mono-tps) in Sitka spruce. We went on to compare this response with the effects of a simulated Insect Attack by drill wounding. A significant increase in mono-tps transcript level was observed in the leaders of lateral branches of weevil-Attacked and mechanically wounded trees. In this study, weevils induced a more rapid enhancement of mono-tps gene expression. A full-length Sitka spruce mono-tps cDNA (PsTPS2) was isolated, expressed in Escherichia coli, and functionally identified as (—)-pinene synthase. The recombinant (—)-pinene synthase catalyzes the formation of (—)-α-pinene and (—)-β-pinene, both of which are known constituents of stem oleoresin in Sitka spruce and increase in abundance after weevil Attack. These data suggest that increased (—)-pinene synthase gene expression is an important element of the direct defense system deployed in Sitka spruce after Insect Attack.
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Insect Attack and Wounding Induce Traumatic Resin Duct Development and Gene Expression of (—)-Pinene Synthase in Sitka Spruce
Plant Physiology, 2003Co-Authors: S. Ashley Byun Mckay, William L Hunter, Kimberleyann Godard, Shawn X Wang, Diane M Martin, Jorg Bohlmann, Aine L PlantAbstract:Conifers possess inducible terpenoid defense systems. These systems are associated with the formation of traumatic resin ducts (TRD) and are underpinned by enhanced gene expression and activity of terpene synthases (TPS), enzymes responsible for oleoresin formation. We first determined that Sitka spruce (Picea sitchensis [Bong.] Carriere) had the capacity for TRD formation by mechanically wounding representative trees. We then proceeded to investigate whether the white pine weevil (Pissodes strobi Peck.), a stem-boring Insect, can influence the expression of genes encoding monoterpene synthases (mono-tps) in Sitka spruce. We went on to compare this response with the effects of a simulated Insect Attack by drill wounding. A significant increase in mono-tps transcript level was observed in the leaders of lateral branches of weevil-Attacked and mechanically wounded trees. In this study, weevils induced a more rapid enhancement of mono-tps gene expression. A full-length Sitka spruce mono-tps cDNA (PsTPS2) was isolated, expressed in Escherichia coli, and functionally identified as (—)-pinene synthase. The recombinant (—)-pinene synthase catalyzes the formation of (—)-α-pinene and (—)-β-pinene, both of which are known constituents of stem oleoresin in Sitka spruce and increase in abundance after weevil Attack. These data suggest that increased (—)-pinene synthase gene expression is an important element of the direct defense system deployed in Sitka spruce after Insect Attack.